茶色素对心肌缺血离体蟾蜍心脏活动的影响
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摘要
目的观察茶色素在心肌缺血状态下对离体蟾蜍心肌收缩力、心电图和心率的影响。方法将60只蟾蜍分为正常蟾蜍离体蛙心组(A组)和心肌缺血蟾蜍离体蛙心组(B组),A组再分为林格液组(A1组)、茶色素低剂量(200mg/L)组(A2组)和茶色素高剂量(400mg/L)组(A3组);B组再分垂体后叶素模型组(B1组)、垂体后叶素+茶色素低剂量(200mg/L)组(B2组)和垂体后叶素+茶色素高剂量(400mg/L)组(B3组)。应用BL-420S生物机能实验系统记录蟾蜍离体心肌收缩力和心电图变化曲线。结果与A1组比较,A3组引起心肌收缩力明显增加(P<0.05),A2组差异无统计学意义(P>0.05);心电图QRS峰峰值和心率差异无统计学意义(P>0.05)。与A1组比较,B1组心肌收缩力、心电图QRS峰峰值和心率均明显下降(P<0.05);与B1组比较,B2、B3组心肌收缩力、心电图QRS峰峰值和心率均明显上升(P<0.05)。结论茶色素能明显改善心肌缺血引起离体蟾蜍心脏功能的下降。
Objective To observe the influence of tea pigment on myocardial contractility,electrocardiogram(ECG)and heart rate in exsomatized toads under the condition of myocardial ischemia.Methods Sixty toads were divided into the normal exsomatized toad heart group(A)and myocardial ischemia toad heart(B).Then the group A was re-divided into the Ringer′s solution group(A1),tea pigment low dose(200 mg/L)group(A2)and the high dose(400 mg/L)group(A3);the group B was re-divided into the pituitrin model group(B1),pituitrin+tea pigment low dose(200 mg/L)group(B2)and high dose(400 mg/L)group(B3).The BL-420 Sbiological function experiment system was used to record the myocardial contractile force and ECG change curve of exsomatized toad.Results Compared with the group A1,the myocardial contractility in the group A3 was obviously increased(P<0.05),the difference in the group A2 had no statistical significance(P>0.05);the QRS peak value of ECG and heart rate had no statistically significant difference(P>0.05);compared with the group A1,the myocardial contractility,ECG QRS peak value and heart rate in the group B1 were significantly decreased(P<0.05);compared with the group B1,the myocardial contractility,ECG QRS peak value and heart rate in the group B2 and B3 were significantly increased(P<0.05).Conclusion Tea pigment can obviously improve the decrease of the exsomatized toad cardiac activity caused by myocardial ischemia.
Objective To observe the influence of tea pigment on myocardial contractility,electrocardiogram(ECG)and heart rate in exsomatized toads under the condition of myocardial ischemia.Methods Sixty toads were divided into the normal exsomatized toad heart group(A)and myocardial ischemia toad heart(B).Then the group A was re-divided into the Ringer′s solution group(A1),tea pigment low dose(200 mg/L)group(A2)and the high dose(400 mg/L)group(A3);the group B was re-divided into the pituitrin model group(B1),pituitrin+tea pigment low dose(200 mg/L)group(B2)and high dose(400 mg/L)group(B3).The BL-420 Sbiological function experiment system was used to record the myocardial contractile force and ECG change curve of exsomatized toad.Results Compared with the group A1,the myocardial contractility in the group A3 was obviously increased(P<0.05),the difference in the group A2 had no statistical significance(P>0.05);the QRS peak value of ECG and heart rate had no statistically significant difference(P>0.05);compared with the group A1,the myocardial contractility,ECG QRS peak value and heart rate in the group B1 were significantly decreased(P<0.05);compared with the group B1,the myocardial contractility,ECG QRS peak value and heart rate in the group B2 and B3 were significantly increased(P<0.05).Conclusion Tea pigment can obviously improve the decrease of the exsomatized toad cardiac activity caused by myocardial ischemia.
引文
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[13]李春美,谢笔钧,姚平.茶色素体外清除氧自由基及对老龄小鼠脂质过氧化作用的影响[J].中国药理学通报,2001,17(2):233-234.
[14]杜荣增,任雨笙,黄高忠,等.茶色素对高血压患者红细胞超氧化物歧化酶和血浆总抗氧化能力的影响[J].现代中西医结合杂志,2004,13(1):13-15.
[15]赵敏,姚优修,李婷,等.茶色素胶囊对兔离体血管平滑肌张力的影响[J].中西医结合心脑血管病杂志,2009,7(12):1448-1449.
[2]卢素芳,王胜鹏.茶色素及其生物活性功能研究[J].湖北农业科学,2015,54(24):6117-6119.
[3]王建枝,殷莲化,吴立玲,等.病理生理学[M].8版.北京:人民卫生出版社,2013:159-161.
[4]董帅,唐泽耀,张冬梅,等.茶色素在低钙及高钙浓度下对离体蟾蜍心肌收缩力的影响[J].中药新药与临床药理,2013,24(6):561-564.
[5]汪洋,唐泽耀,王珂萌,等.茶色素对离体蟾蜍心肌收缩力的保护作用[J].中国实验方剂学杂志,2015,21(12):90-94.
[6]刘克敏,孙艺平,王珂萌,等.鹿茸的心肌保护作用与M受体的相关性研究[J].重庆医学,2016,45(31):4404-4406.
[7]刘展,马红萍,彭聿平.垂体后叶素诱发离体蛙心心肌缺血实验模型[J].交通医学,2010,24(5):466-467.
[8]崔艳秋,王俊雅,张冬梅,等.蛙心灌流模型的改进[J].首都医科大学学报,2005,26(2):229-231.
[9]罗永明,李诒光.茶色素的化学成分研究[J].中草药,2002,33(12):1066-1067.
[10]邵翔,杨浩,俞国华.茶提取物在心血管疾病中的应用[J].现代中西医结合杂志,2005,14(14):1917-1919.
[11]施新献.现代医学实用动物学[M].北京:人民军医出版社,2000:442-444.
[12]简文亭,简道林,马会改.迷走神经刺激在心肌缺血再灌注损伤中的研究进展[J].广东医学,2013,34(12):1938-1940.
[13]李春美,谢笔钧,姚平.茶色素体外清除氧自由基及对老龄小鼠脂质过氧化作用的影响[J].中国药理学通报,2001,17(2):233-234.
[14]杜荣增,任雨笙,黄高忠,等.茶色素对高血压患者红细胞超氧化物歧化酶和血浆总抗氧化能力的影响[J].现代中西医结合杂志,2004,13(1):13-15.
[15]赵敏,姚优修,李婷,等.茶色素胶囊对兔离体血管平滑肌张力的影响[J].中西医结合心脑血管病杂志,2009,7(12):1448-1449.