鱼腥草酚类物质累积对土壤环境因子的响应
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摘要
为探索不同来源野生鱼腥草酚类物质累积对土壤环境因子的响应,提高鱼腥草的品质。通过野外采样,分析了贵州贵定、惠水及丹寨3县11个样地的鱼腥草叶片酚类物质累积与土壤环境因子的相关性。结果显示不同来源鱼腥草酚类物质的累积量差异明显,叶片绿原酸、芦丁、异槲皮苷、阿福豆苷、槲皮苷的累积量变化分别在37. 5～267. 7μg/g、67. 5～329. 1μg/g、61. 4～287. 8μg/g、363. 5～1 073. 8μg/g、382. 6～876. 9μg/g之间;土壤养分、p H、酶活性也因采样地的不同而存在差异;影响鱼腥草酚类物质累积的主要因子有土壤有机质、全氮及全钾含量,其次为土壤p H、蔗糖酶活性及全磷、速效钾含量。该结果可为鱼腥草的栽培地选择及提高品质提供参考。
Plant and soil samples of wild Houttuynia cordata Thunb. were collected from 11 different sites( Guiding country,Huishui country and Danzhai country,in Guizhou province) and correlations of H. cordata phenolics and soil environmental factors were analyzed. The aim was to explore response of accumulation of H. cordata phenolics to soil environmental factors. The results showed that accumulative contents of H. cordata phenolics from different sites were significantly different. The ranges about contents of chlorogenic acid,rutin,afzelin,quercitrin,and isoquercitrin of H. cordata leaves were 37. 5 ～ 267. 7 μg/g,67. 5 ～ 329. 1 μg/g,61. 4 ～ 287. 8 μg/g,363. 5 ～ 1 073. 8 μg/g,382. 6～ 876. 9 μg/g,respectively. Soil nutrient contents,pH,and enzyme activities were significantly different with various sampling sites. The main factors,which influenced accumulation of H. cordata phenolics,were the contents of soil organic matters,total nitrogen and total potassium. The secondary factors were soil pH,sucrase activities,total phosphorus,and available potassium. The results of this study provide important references for the improving quality and selection of place where H. cordata is grown.
Plant and soil samples of wild Houttuynia cordata Thunb. were collected from 11 different sites( Guiding country,Huishui country and Danzhai country,in Guizhou province) and correlations of H. cordata phenolics and soil environmental factors were analyzed. The aim was to explore response of accumulation of H. cordata phenolics to soil environmental factors. The results showed that accumulative contents of H. cordata phenolics from different sites were significantly different. The ranges about contents of chlorogenic acid,rutin,afzelin,quercitrin,and isoquercitrin of H. cordata leaves were 37. 5 ～ 267. 7 μg/g,67. 5 ～ 329. 1 μg/g,61. 4 ～ 287. 8 μg/g,363. 5 ～ 1 073. 8 μg/g,382. 6～ 876. 9 μg/g,respectively. Soil nutrient contents,pH,and enzyme activities were significantly different with various sampling sites. The main factors,which influenced accumulation of H. cordata phenolics,were the contents of soil organic matters,total nitrogen and total potassium. The secondary factors were soil pH,sucrase activities,total phosphorus,and available potassium. The results of this study provide important references for the improving quality and selection of place where H. cordata is grown.
引文
[1]林开敏,叶发茂,林艳,等.酚性物质对土壤和植物的作用机制研究进展[J].中国生态农业学报,2010(5):1130-1137.
[2]国家药典委员会.中国药典(2015年版第一部)[M].北京:中国医药科技出版社,2015:224.
[3]童文,李敏,孙佩,等.药食同源植物鱼腥草研究现状[J].辽宁中医药大学学报,2018,20(6):183-185.
[4]麦明朗,余林中,刘俊珊.“中药抗生素”鱼腥草抗炎作用研究及临床应用进展[J].中药药理与临床,2018,34(5):172-176.
[5]刘玉红,李青苗,黄志芳,等. GC法测定鱼腥草挥发油中α-蒎烯、β-蒎烯等7种成分的含量[J].药物分析杂志,2015,35(10):1810-1814.
[6]梁慧超,孟江,卢国勇,等.鱼腥草总生物碱提取工艺的研究[J].成都医学院学报,2010,5(4):311-313.
[7]杨占南.鱼腥草(Houttuyni acordata Thunb.)酚类及挥发性物质代谢累积与土壤环境因子的相关性研究[D].北碚:西南大学,2013.
[8]张思荻,何金晓,施佳,等.基于4种挥发性成分对鱼腥草种质资源的初步评价研究[J].中药与临床,2018,9(2):3-6.
[9]李瑞玲.鱼腥草中鱼腥草素提取工艺优化[J].南阳理工学院学报,2016(4):123-125.
[10]杨占南,罗世琼,孙一铭,等.鱼腥草酚类物质及其与土壤酶活性的相关性分析[J].广东农业科学,2012(22):119-124.
[11]鲍士旦.土壤农化分析(第三版)[M].北京:中国农业出版社,1981.
[12]关松荫.土壤酶及其研究方法[M].北京:中国农业出版社,1986.
[13]MENG J,DONG X P,ZHOU Y S,et al. Studies on chemical constituents of phenols in fresh Houttuynia cordata[J]. China Journal of Chinese Materia,2007,32:929-931.
[14]TODA S. Antioxidative effects of polyphenols in leaves of Houttuynia cordata on protein fragmentation by copperhydrogen peroxide in vitro[J]. Journal of medicinal Food,2005,8:266-268.
[15]赵铖,杨占南,罗世琼,等.土壤养分对鱼腥草挥发性成分的影响[J].江苏农业科学,2018,46(9):187-190.
[16]吕卫光,沈其荣,余廷园,等.酚酸化合物对土壤酶活性和土壤养分的影响[J].植物营养与肥料学报,2006(6):845-849.
[17]王雪飞,张华.多酚生理功能的研究进展[J].食品研究与研发,2012(2):211-212.
[2]国家药典委员会.中国药典(2015年版第一部)[M].北京:中国医药科技出版社,2015:224.
[3]童文,李敏,孙佩,等.药食同源植物鱼腥草研究现状[J].辽宁中医药大学学报,2018,20(6):183-185.
[4]麦明朗,余林中,刘俊珊.“中药抗生素”鱼腥草抗炎作用研究及临床应用进展[J].中药药理与临床,2018,34(5):172-176.
[5]刘玉红,李青苗,黄志芳,等. GC法测定鱼腥草挥发油中α-蒎烯、β-蒎烯等7种成分的含量[J].药物分析杂志,2015,35(10):1810-1814.
[6]梁慧超,孟江,卢国勇,等.鱼腥草总生物碱提取工艺的研究[J].成都医学院学报,2010,5(4):311-313.
[7]杨占南.鱼腥草(Houttuyni acordata Thunb.)酚类及挥发性物质代谢累积与土壤环境因子的相关性研究[D].北碚:西南大学,2013.
[8]张思荻,何金晓,施佳,等.基于4种挥发性成分对鱼腥草种质资源的初步评价研究[J].中药与临床,2018,9(2):3-6.
[9]李瑞玲.鱼腥草中鱼腥草素提取工艺优化[J].南阳理工学院学报,2016(4):123-125.
[10]杨占南,罗世琼,孙一铭,等.鱼腥草酚类物质及其与土壤酶活性的相关性分析[J].广东农业科学,2012(22):119-124.
[11]鲍士旦.土壤农化分析(第三版)[M].北京:中国农业出版社,1981.
[12]关松荫.土壤酶及其研究方法[M].北京:中国农业出版社,1986.
[13]MENG J,DONG X P,ZHOU Y S,et al. Studies on chemical constituents of phenols in fresh Houttuynia cordata[J]. China Journal of Chinese Materia,2007,32:929-931.
[14]TODA S. Antioxidative effects of polyphenols in leaves of Houttuynia cordata on protein fragmentation by copperhydrogen peroxide in vitro[J]. Journal of medicinal Food,2005,8:266-268.
[15]赵铖,杨占南,罗世琼,等.土壤养分对鱼腥草挥发性成分的影响[J].江苏农业科学,2018,46(9):187-190.
[16]吕卫光,沈其荣,余廷园,等.酚酸化合物对土壤酶活性和土壤养分的影响[J].植物营养与肥料学报,2006(6):845-849.
[17]王雪飞,张华.多酚生理功能的研究进展[J].食品研究与研发,2012(2):211-212.