质谱法检测食源性致病菌技术研究进展
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摘要
食源性致病菌存在广泛,能够引起人类的疾病甚至死亡,研究发现超过一半的食品安全问题来源于食源性致病菌的污染。如何快速有效地检测出食源性致病菌是预防和控制食品安全问题的关键环节。系统地介绍了检测食源性致病菌的方法,包括传统培养法、代谢学法、分子生物学法、免疫学方法等传统方法以及新兴的质谱法。质谱法有检测效率高、操作简便、灵敏度高等优点,着重对质谱法的原理、应用以及未来的发展趋势进行了阐述,以期为该技术的研究开发和推广应用提供参考。
Foodborne pathogens widely exist and can cause human disease and even death. The study has found that more than half of food safety problems stem from the contamination of foodborne pathogens. Therefore,how to detect food-borne pathogens rapidly and effectively is a key link in the preventing and controlling food safety. This paper systematically reviewed the methods of detecting foodborne pathogens,including traditional methods such as traditional culture method,metabolic method,molecular biological method and immunological method,and the new mass spectrometry method. Since the mass spectrometry has the advantages of high detection efficiency,simple to operate,and high sensitivity,hence,this paper focuses on the principle,application and future development trend of mass spectrometry,hopefully to provide reference for the research and development and popularized the application of the technology.
Foodborne pathogens widely exist and can cause human disease and even death. The study has found that more than half of food safety problems stem from the contamination of foodborne pathogens. Therefore,how to detect food-borne pathogens rapidly and effectively is a key link in the preventing and controlling food safety. This paper systematically reviewed the methods of detecting foodborne pathogens,including traditional methods such as traditional culture method,metabolic method,molecular biological method and immunological method,and the new mass spectrometry method. Since the mass spectrometry has the advantages of high detection efficiency,simple to operate,and high sensitivity,hence,this paper focuses on the principle,application and future development trend of mass spectrometry,hopefully to provide reference for the research and development and popularized the application of the technology.
引文
[1]陈庆森,冯永强,黄宝华,等.食品中致病菌的快速检测技术的研究现状与进展[J].食品科学,2003,24(11):148-152.
[2]Olopoenia LA,King AL.Widal agglutination test-100 years later:still plagued by controversy[J].Postgraduate medical journal,2000,76(892):80-84.
[3]Cevallos-Cevallos JM,Reyes-De-Corcuera JI,Etxeberria E,et al.Metabolomic analysis in food science:a review[J].Trends in Food Science&Technology,2009,20(11):557-566.
[4]刘继青,郜春花.DNA探针及基因放大技术对食品中微生物类致病菌的快速检测[J].食品科学,1996,17(8):47-50.
[5]樊慧珍,于化鹏,黄文杰.DNA探针杂交快速检测肺炎链球菌和流感嗜血杆菌[J].南方医科大学学报,2005,25(12):1503-1506.
[6]吴仲梁,李晓虹,韩伟,等.利用商品DNA探针对食品中单核细胞增生李斯特菌的快速检测评估[J].中国人兽共患病杂志,2002,18(5):64-68.
[7]罗燕萍.质谱技术在临床微生物实验室中的应用前景[J].检验医学,2015,30(2):97-101.
[8]周军芳.质谱技术及其在临床微生物鉴定中的应用[J].中国中医药咨讯,2010,2(9):52.
[9]Anhalt JP,Fenselau C.Identification of bacteria using mass spectrometry[J].Analytical Chemistry,1975,47(2):219-225.
[10]Claydon MA,Davey SN,Edwards-Jones V,et al.The rapid identification of intact microorganisms using mass spectrometry[J].Nature biotechnology,1996,14(11):1584-1586.
[11]Schmid O,Ball G,Lancashire L,et al.New approaches to identification of bacterial pathogens by surface enhanced laser desorption/ionization time of flight mass spectrometry in concert with artificial neural networks,with special reference to Neisseria gonorrhoeae[J].Journal of medical microbiology,2005,54(12):1205-1211.
[12]孙宗科,张伟,陈西平.基质辅助激光解析电离飞行时间质谱与细菌的快速鉴定[J].国外医学:微生物学分册,2003,26(5):14-15.
[13]Holland RD,Duffy CR,Rafii F,et al.Identification of bacterial proteins observed in MALDI TOF mass spectra from whole cells[J].Analytical Chemistry,1999,71(15):3226-3230.
[14]嵇金如,沈萍,应超群,等.基质辅助激光解吸电离飞行时间质谱在醋酸钙-鲍曼不动杆菌复合菌鉴定及同源性分析中的应用[J].中国抗生素杂志,2017,42(2):134-138.
[15]肖代雯,杨永长,刘华,等.用表面增强激光解析/电离飞行时间质谱蛋白指纹快速鉴定4种临床常见菌[J].中华微生物学和免疫学杂志,2012,(6):566-570.
[16]Arnold RJ,Karty JA,Ellington AD,et al.Monitoring the growth of a bacteria culture by MALDI-MS of whole cells[J].Analytical Chemistry,1999,71(10):1990-1996.
[17]Bright JJ,Claydon MA,Soufian M,et al.Rapid typing of bacteria using matrix-assisted laser desorption ionisation time-offlight mass spectrometry and pattern recognition software[J].Journal of microbiological methods,2002,48(2):127-138.
[18]周千渝,张延国,黄成才,等.食源性致病菌MALDI-TOF MS检测方法的建立与应用[J].食品工业科技,2015,36(18):59-64.
[19]何英,陆学东.基质辅助激光解吸电离飞行时间质谱技术及其在临床微生物鉴定中的应用[J].中华检验医学杂志,2011,34(10):881-888.
[20]李楠,刘文森,刘林娜,等.MALDI-TOF质谱技术及Biotyper数据库在养殖场空气微生物鉴定中的应用[J].中国兽医学报,2014,34(12):2031-2034.
[21]陈飞,胡玢婕,赵虎.MALDI-TOF MS在临床微生物样本直接检测中的应用[J].检验医学,2015,30(7):750-756.
[22]饶名祯,顾晨荣,李云霞,等.MALDI-TOF MS技术在食品微生物领域的应用研究[J].上海师范大学学报(自然科学版),2015,44(6):681-686.
[23]谢小芳,周惠琴,郑毅,等.基质辅助激光解吸电离飞行时间质谱技术直接鉴定血流感染病原菌的效果评价[J].中华临床感染病杂志,2016,9(2):152-155.
[24]Mellmann A,Cloud J,Maier T,et al.Evaluation of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry in comparison to 16S rRNA gene sequencing for species identification of nonfermenting bacteria[J].Journal of clinical microbiology,2008,46(6):1946-1954.
[25]王晔茹,崔生辉,李凤琴.基质辅助激光解吸/电离飞行时间质谱在沙门菌检测和鉴定分型中的应用研究[J].卫生研究,2008,37(6):685-689.
[2]Olopoenia LA,King AL.Widal agglutination test-100 years later:still plagued by controversy[J].Postgraduate medical journal,2000,76(892):80-84.
[3]Cevallos-Cevallos JM,Reyes-De-Corcuera JI,Etxeberria E,et al.Metabolomic analysis in food science:a review[J].Trends in Food Science&Technology,2009,20(11):557-566.
[4]刘继青,郜春花.DNA探针及基因放大技术对食品中微生物类致病菌的快速检测[J].食品科学,1996,17(8):47-50.
[5]樊慧珍,于化鹏,黄文杰.DNA探针杂交快速检测肺炎链球菌和流感嗜血杆菌[J].南方医科大学学报,2005,25(12):1503-1506.
[6]吴仲梁,李晓虹,韩伟,等.利用商品DNA探针对食品中单核细胞增生李斯特菌的快速检测评估[J].中国人兽共患病杂志,2002,18(5):64-68.
[7]罗燕萍.质谱技术在临床微生物实验室中的应用前景[J].检验医学,2015,30(2):97-101.
[8]周军芳.质谱技术及其在临床微生物鉴定中的应用[J].中国中医药咨讯,2010,2(9):52.
[9]Anhalt JP,Fenselau C.Identification of bacteria using mass spectrometry[J].Analytical Chemistry,1975,47(2):219-225.
[10]Claydon MA,Davey SN,Edwards-Jones V,et al.The rapid identification of intact microorganisms using mass spectrometry[J].Nature biotechnology,1996,14(11):1584-1586.
[11]Schmid O,Ball G,Lancashire L,et al.New approaches to identification of bacterial pathogens by surface enhanced laser desorption/ionization time of flight mass spectrometry in concert with artificial neural networks,with special reference to Neisseria gonorrhoeae[J].Journal of medical microbiology,2005,54(12):1205-1211.
[12]孙宗科,张伟,陈西平.基质辅助激光解析电离飞行时间质谱与细菌的快速鉴定[J].国外医学:微生物学分册,2003,26(5):14-15.
[13]Holland RD,Duffy CR,Rafii F,et al.Identification of bacterial proteins observed in MALDI TOF mass spectra from whole cells[J].Analytical Chemistry,1999,71(15):3226-3230.
[14]嵇金如,沈萍,应超群,等.基质辅助激光解吸电离飞行时间质谱在醋酸钙-鲍曼不动杆菌复合菌鉴定及同源性分析中的应用[J].中国抗生素杂志,2017,42(2):134-138.
[15]肖代雯,杨永长,刘华,等.用表面增强激光解析/电离飞行时间质谱蛋白指纹快速鉴定4种临床常见菌[J].中华微生物学和免疫学杂志,2012,(6):566-570.
[16]Arnold RJ,Karty JA,Ellington AD,et al.Monitoring the growth of a bacteria culture by MALDI-MS of whole cells[J].Analytical Chemistry,1999,71(10):1990-1996.
[17]Bright JJ,Claydon MA,Soufian M,et al.Rapid typing of bacteria using matrix-assisted laser desorption ionisation time-offlight mass spectrometry and pattern recognition software[J].Journal of microbiological methods,2002,48(2):127-138.
[18]周千渝,张延国,黄成才,等.食源性致病菌MALDI-TOF MS检测方法的建立与应用[J].食品工业科技,2015,36(18):59-64.
[19]何英,陆学东.基质辅助激光解吸电离飞行时间质谱技术及其在临床微生物鉴定中的应用[J].中华检验医学杂志,2011,34(10):881-888.
[20]李楠,刘文森,刘林娜,等.MALDI-TOF质谱技术及Biotyper数据库在养殖场空气微生物鉴定中的应用[J].中国兽医学报,2014,34(12):2031-2034.
[21]陈飞,胡玢婕,赵虎.MALDI-TOF MS在临床微生物样本直接检测中的应用[J].检验医学,2015,30(7):750-756.
[22]饶名祯,顾晨荣,李云霞,等.MALDI-TOF MS技术在食品微生物领域的应用研究[J].上海师范大学学报(自然科学版),2015,44(6):681-686.
[23]谢小芳,周惠琴,郑毅,等.基质辅助激光解吸电离飞行时间质谱技术直接鉴定血流感染病原菌的效果评价[J].中华临床感染病杂志,2016,9(2):152-155.
[24]Mellmann A,Cloud J,Maier T,et al.Evaluation of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry in comparison to 16S rRNA gene sequencing for species identification of nonfermenting bacteria[J].Journal of clinical microbiology,2008,46(6):1946-1954.
[25]王晔茹,崔生辉,李凤琴.基质辅助激光解吸/电离飞行时间质谱在沙门菌检测和鉴定分型中的应用研究[J].卫生研究,2008,37(6):685-689.