食品能力验证中沙门氏菌的分离鉴定和分型
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摘要
为了提高微生物实验室沙门氏菌的检验能力,提升实验室检验水平。通过对比全自动荧光酶联免疫检测仪(miniVIDAS)和BAX~ System Q7病原微生物快速检测系统进行快速筛选,并辅以全自动微生物基因指纹鉴定系统(RiboPrinter)进行菌种鉴定的方法,与国标方法 GB 4789.4-2016《食品安全国家标准食品微生物学检验沙门氏菌检验》进行沙门氏菌生化和血清检验。试验通过miniVIDAS检测和BAX~ System Q7病原微生物快速检测系统检测与国标方法结果一致,编号为CODE:028和CODE:069的样品分别检出鼠伤寒沙门氏菌。miniVIDAS检测和BAX~ System Q7检测在检测沙门氏菌方面操作简单快速,检测效率高。能力验证在国标法的基础上可以采用以上方法进行平行试验,使结果更加准确可靠。
To improve the testing ability of Salmonella in microbial laboratory,Salmonella biochemical and serological tests were carried out according to GB 4789.4-2016,automated fluorescent enzyme-linked immunosorbent assay(miniVIDAS)and BAX~System Q7 rapid pathogen detection system were applied for rapid screening,identification of strain was implemented by automatic microbial genetic fingerprint identification system(RiboPrinter)for supplement. Salmonella typhimuruim was detected in both sample CODE:028 and CODE:069,this experiment through miniVIDAS detection and BAX~System Q7 microbial rapid detection system detection ran parallel to national standard method results. miniVIDAS and BAX~System Q7 rapid detection system of pathogenic microorganisms reveal their convenient operation and high detection efficiency in the detection of Salmonella sample. On the basis of the national standard method,the capability verification can be carried out in parallel experiments with the above methods to acquire more accurate and reliable results.
To improve the testing ability of Salmonella in microbial laboratory,Salmonella biochemical and serological tests were carried out according to GB 4789.4-2016,automated fluorescent enzyme-linked immunosorbent assay(miniVIDAS)and BAX~System Q7 rapid pathogen detection system were applied for rapid screening,identification of strain was implemented by automatic microbial genetic fingerprint identification system(RiboPrinter)for supplement. Salmonella typhimuruim was detected in both sample CODE:028 and CODE:069,this experiment through miniVIDAS detection and BAX~System Q7 microbial rapid detection system detection ran parallel to national standard method results. miniVIDAS and BAX~System Q7 rapid detection system of pathogenic microorganisms reveal their convenient operation and high detection efficiency in the detection of Salmonella sample. On the basis of the national standard method,the capability verification can be carried out in parallel experiments with the above methods to acquire more accurate and reliable results.
引文
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[2]王学硕,崔生辉,邢书霞,等.餐饮食品中沙门氏菌的危害分析、污染调查与防控[J].中国药事,2013,27(9):974-979
[3]江志杰,王似锦,高春.食品中沙门氏菌检出能力验证结果与分析[J].食品安全质量检测学报,2015,6(5):1929-1935
[4]中华人民共和国国家卫生和计划生育委员会.GB 29921-2013食品安全国家标准食品中致病菌限量[S].北京:中国标准出版社,2013:2-3
[5]中国合格评定国家认可委员会.2016能力验证规则:CNAS-RL02[S].北京:中国合格评定国家认可委员会,2016:2-3
[6]舒鹃娟,张伟冲,袁峰,等.食品微生物的检测能力验证[J].上海预防医学,2014,26(3):154-157
[7]朱超,许学斌.沙门菌属血清型诊断[M].上海:同济大学出版社,2009:10-12
[8]国家卫生和计划生育委员会.GB 4789.4-2016食品安全国家标准食品微生物学检验沙门氏菌检验[S].北京:中国标准出版社,2016:3-15
[9]张艳超,史永刚,卫佳欢,等.3种方法检测食品中沙门菌能力验证结果分析[J].检验检疫学刊,2016,26(3):14-17
[10]陈茂义,胡婕,刘建昭,等.科玛嘉显色培养基和XLD、SS、HE分离食品中沙门菌效果比较[J].公共卫生与预防医学,2008,19(4):12-14
[11]李志勇,谢钧宪,许龙岩,等.食品微生物检验的质量控制[J].检验检疫科学,2004,14(4):5-9
[12]李海丽,柯娴,邓金兰,等.沙门氏菌检测方法的优化[J].中外食品工业,2013(12):23-24
[13]刘慧玲,万志刚,洪小柳,等.进出口食品中不同血清型沙门氏菌PFGE和MLST分型比较研究[J].食品安全质量检测学报,2014,5(11):3454-3461
[14]饶名祯,顾晨荣,李云霞,等.MALDI-TOF MS技术在食品微生物领域的应用研究[J].上海师范大学学报(自然科学版),2015,44(6):681-686
[15]苑红丽.基因检测技术在病原微生物检测及微生物质控中的应用[J].现代科学仪器,2006,16(1):42-44