微生物法测定婴幼儿乳粉中烟酸含量
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摘要
目的建立微生物法测定婴幼儿乳粉中烟酸含量的分析方法。方法通过比较不同浓度标准曲线对同一标准物质样品的测定结果,确定准确最优的线性浓度范围,并在该标准范围内进行重复性及加标回收实验。结果当烟酸标曲线性浓度范围为5.0～50.0ng时,线性关系良好(r~2=0.996)。该方法的相对标准偏差(relative standard deviation, RSD)为1.71%(n=6),平均回收率为94.4%。结论该方法稳定准确,适合用于测定婴幼儿乳粉中烟酸的含量。
Objective To establish a method for the determination of niacin in infant milk powder by microbiological method. Methods By comparing the measurement results of the same standard substance sample with different concentration standard curves, the accurate and optimal linear concentration range was determined, and the repeatability and spike recovery test were carried out within the standard range. Results When the nicotinic acid standard concentration range was 5.0-50.0 ng, the linear relationship was good(r~2=0.996). The relative standard deviation(RSD) of the method was 1.71%(n=6), and the average recovery was 94.4%. Conclusion The method is stable and accurate, which is suitable for determining the content of niacin in infant milk powder.
Objective To establish a method for the determination of niacin in infant milk powder by microbiological method. Methods By comparing the measurement results of the same standard substance sample with different concentration standard curves, the accurate and optimal linear concentration range was determined, and the repeatability and spike recovery test were carried out within the standard range. Results When the nicotinic acid standard concentration range was 5.0-50.0 ng, the linear relationship was good(r~2=0.996). The relative standard deviation(RSD) of the method was 1.71%(n=6), and the average recovery was 94.4%. Conclusion The method is stable and accurate, which is suitable for determining the content of niacin in infant milk powder.
引文
[1]Moreschi ECP,Matos JR,Almeida-Muradian LB.Thermal analysis of vitamin PP niacin and niacinamide[J].J Therm Anal Calorim,2009,98(1):161-164.
[2]Katrina L,Bogan,Charles B.Nicotinic acid,nicotinamide,and nicotinamide riboside:A molecular evaluation of NAD+precursor vitamins in human nutrition[J].Annu Rev Nutr,2008,(28):115-130.
[3]Carlson LA.Nicotinic acid:The broad-spectrum lipid drug.A50th anniversary review[J].J Int Med,2005,25(8):94-114.
[4]樊继鹏,徐振东,陈军,等.高效液相色谱法同时测定乳粉中烟酸、烟酰胺和维生素B6[J].包装与食品机械,2015,(3):66-69.Fan JP,Xu ZD,Chen J,et al.HPLC Determination of niacin,nicotinamide and vitamin B6 in milk powder[J].Packag Food Mach,2015,(3):66-69.
[5]郝刚,俞蕴莉,顾炳仁.UPLC-MS/MS法测定奶粉中烟酸的含量[J].中国乳业,2014,(11):54-57,58.Hao G,Yu YL,Gu BR.Determination of nicotinic acid in milk powder by ultra performance liquid chromatography-tandem mass spectrometry[J].China Dair,2014,(11):54-57,58.
[6]李钟玉,李临生.烟酸、烟酰胺的研究进展[J].化工时刊,2003,(2):6-9.Li ZY,Li LS.Progress on research of nicotinic acid and nicotinamide[J].Chem Ind Tim,2003,(2):6-9.
[7]刘志楠,李海礁,宋晓东,等.牛奶中烟酸本底含量的测定[J].食品研究与开发,2012,(10):103-106.Liu ZN,Li HQ,Song XD,et al.Determination of the niacin in milk[J].Food Res Dev,2012,(10):103-106.
[8]GB 5009.89-2016食品安全国家标准食品中烟酸和烟酰胺的测定[S].GB 5009.89-2016 National food safety standard-Determination of vitamin niacin and niacinamide in foods[S].
[9]张娟,严家俊,吴风霞,等.食品中维生素B12和叶酸微生物检测法的研究进展[J].食品工业科技,2018,39(24):346-349.Zhang J,Yan JJ,Wu FX,et al.Research progress of the microbiological detection technology for vitamin B12 and folic acid in food[J].Sci Technol Food Ind,2018,39(24):346-349.
[10]殷晓红,杨金宝,刘波,等.微生物法测定食品中的烟酸和烟酰胺[J].中国乳品工业,2003,(2):32-35.Yin XH,Yang JB,Liu B,et al.Determination method for niacin in infants formula food[J].China Dair Ind,2003,(2):32-35.
[11]王志伟.婴幼儿乳粉中生物素含量的测定研究[J].包装与食品机械,2013,(3):66-68,72.Wang ZW.Research on determination of biotin in infant milk powder[J].Packag Food Mach,2013,(3):66-68,72.
[12]胡玉霞,汪立明,王静,等.高效液相色谱法测定婴幼儿配方米粉和乳粉中烟酸和烟酰胺含量[J].食品安全质量检测学报,2016,(2):839-845.Hu YX,Wang LM,Wang J,et al.Determination of nicotinic acid and nicotinamide in infant formula milk powder and rice powder by high performance liquid chromatography[J].J Food Saf Qual,2016,(2):839-845.
[13]韩军花,陈龙,梁栋,等.我国婴幼儿配方食品标准中维生素适宜范围值探讨[J].食品科学技术学报,2018,36(3):11-17.Han JH,Chen L,Liang D,et al.Study on appropriate values of vitamins in infant and follow-up formula standard of China[J].J Food Sci Technol,2018,36(3):11-17.
[14]GB 10767-2010食品安全国家标准较大婴儿和幼儿配方食品[S].GB 10767-2010 National food safety standard-Older infants and young children formula[S].
[15]GB 10765-2010食品安全国家标准婴儿配方食品[S].GB 10765-2010 National food safety standard-Infant formula[S].
[2]Katrina L,Bogan,Charles B.Nicotinic acid,nicotinamide,and nicotinamide riboside:A molecular evaluation of NAD+precursor vitamins in human nutrition[J].Annu Rev Nutr,2008,(28):115-130.
[3]Carlson LA.Nicotinic acid:The broad-spectrum lipid drug.A50th anniversary review[J].J Int Med,2005,25(8):94-114.
[4]樊继鹏,徐振东,陈军,等.高效液相色谱法同时测定乳粉中烟酸、烟酰胺和维生素B6[J].包装与食品机械,2015,(3):66-69.Fan JP,Xu ZD,Chen J,et al.HPLC Determination of niacin,nicotinamide and vitamin B6 in milk powder[J].Packag Food Mach,2015,(3):66-69.
[5]郝刚,俞蕴莉,顾炳仁.UPLC-MS/MS法测定奶粉中烟酸的含量[J].中国乳业,2014,(11):54-57,58.Hao G,Yu YL,Gu BR.Determination of nicotinic acid in milk powder by ultra performance liquid chromatography-tandem mass spectrometry[J].China Dair,2014,(11):54-57,58.
[6]李钟玉,李临生.烟酸、烟酰胺的研究进展[J].化工时刊,2003,(2):6-9.Li ZY,Li LS.Progress on research of nicotinic acid and nicotinamide[J].Chem Ind Tim,2003,(2):6-9.
[7]刘志楠,李海礁,宋晓东,等.牛奶中烟酸本底含量的测定[J].食品研究与开发,2012,(10):103-106.Liu ZN,Li HQ,Song XD,et al.Determination of the niacin in milk[J].Food Res Dev,2012,(10):103-106.
[8]GB 5009.89-2016食品安全国家标准食品中烟酸和烟酰胺的测定[S].GB 5009.89-2016 National food safety standard-Determination of vitamin niacin and niacinamide in foods[S].
[9]张娟,严家俊,吴风霞,等.食品中维生素B12和叶酸微生物检测法的研究进展[J].食品工业科技,2018,39(24):346-349.Zhang J,Yan JJ,Wu FX,et al.Research progress of the microbiological detection technology for vitamin B12 and folic acid in food[J].Sci Technol Food Ind,2018,39(24):346-349.
[10]殷晓红,杨金宝,刘波,等.微生物法测定食品中的烟酸和烟酰胺[J].中国乳品工业,2003,(2):32-35.Yin XH,Yang JB,Liu B,et al.Determination method for niacin in infants formula food[J].China Dair Ind,2003,(2):32-35.
[11]王志伟.婴幼儿乳粉中生物素含量的测定研究[J].包装与食品机械,2013,(3):66-68,72.Wang ZW.Research on determination of biotin in infant milk powder[J].Packag Food Mach,2013,(3):66-68,72.
[12]胡玉霞,汪立明,王静,等.高效液相色谱法测定婴幼儿配方米粉和乳粉中烟酸和烟酰胺含量[J].食品安全质量检测学报,2016,(2):839-845.Hu YX,Wang LM,Wang J,et al.Determination of nicotinic acid and nicotinamide in infant formula milk powder and rice powder by high performance liquid chromatography[J].J Food Saf Qual,2016,(2):839-845.
[13]韩军花,陈龙,梁栋,等.我国婴幼儿配方食品标准中维生素适宜范围值探讨[J].食品科学技术学报,2018,36(3):11-17.Han JH,Chen L,Liang D,et al.Study on appropriate values of vitamins in infant and follow-up formula standard of China[J].J Food Sci Technol,2018,36(3):11-17.
[14]GB 10767-2010食品安全国家标准较大婴儿和幼儿配方食品[S].GB 10767-2010 National food safety standard-Older infants and young children formula[S].
[15]GB 10765-2010食品安全国家标准婴儿配方食品[S].GB 10765-2010 National food safety standard-Infant formula[S].