有核红细胞采用XN1000血液分析仪计数与人工计数结果比较
|
摘要
目的 Sysmex XN1000b型全自动血细胞分析仪检测的有核红细胞结果的有效性和准确性,通过金标法人工涂片检测进行验证。方法 2017年1月~2017年3月本院经过Sysmex XN1000b型全自动血细胞分析仪检测有核红细胞结果阳性标本213例进行仪器结果与人工涂片结果进行准确性验证,对随机抽取100例有核红细胞结果为零的标本验证阴性符合率验证。结果采用Sysmex XN1000b型全自动血细胞分析仪计数有核红细胞,真阳性率100.00%,假阳性率2.09%,假阴性率0(<5%),真阴性率96.70%,阳性结果符合率97.56%。结论 Sysmex XN1000b型全自动血细胞分析仪检测的有核红细胞计数准确性高,阴阳性符合率好,结果可靠,有核红细胞计数可以作为临床报告项目。
Objective To evaluate the validity and accuracy of Sysmex XN1000 b automatic blood cell analyzer for the detection of nucleated red blood cells.Methods From January 2017 to March 2017,213 patients with positive results of nucleated erythrocytes were detected by Sysmex XN1000 b automatic hematology analyzer.The results of the instrument and the results of artificial smear were verified.A total of 100 cases of nucleated erythrocytes negative results of the sample validation negative validation.Results The nucleated erythrocytes were counted by Sysmex XN1000 b automatic hematology analyzer.The true positive rate was 100%,the false positive rate was 2.09%,the false negative rate was 0(<5%),the true negative rate was 97.10%,and the positive rate was 96.70%.Conclusion Sysmex XN1000 b automatic hematology analyzer detected high accuracy of nucleated red blood cell count,positive and negative coincidence rate,the results are reliable,the number of nucleated red blood cells can be used as a clinical report items.
Objective To evaluate the validity and accuracy of Sysmex XN1000 b automatic blood cell analyzer for the detection of nucleated red blood cells.Methods From January 2017 to March 2017,213 patients with positive results of nucleated erythrocytes were detected by Sysmex XN1000 b automatic hematology analyzer.The results of the instrument and the results of artificial smear were verified.A total of 100 cases of nucleated erythrocytes negative results of the sample validation negative validation.Results The nucleated erythrocytes were counted by Sysmex XN1000 b automatic hematology analyzer.The true positive rate was 100%,the false positive rate was 2.09%,the false negative rate was 0(<5%),the true negative rate was 97.10%,and the positive rate was 96.70%.Conclusion Sysmex XN1000 b automatic hematology analyzer detected high accuracy of nucleated red blood cell count,positive and negative coincidence rate,the results are reliable,the number of nucleated red blood cells can be used as a clinical report items.
引文
[1]陆作洁,农少云.外周血涂片中出现有核红细胞的临床意义探讨[J].国际检验医学杂志,2013,34(19):2605-2607.
[2]王也飞,周怡,丁磊,等.SYSMEX XN-1000全自动血液分析仪计数有核红细胞的准确性评价[J].检验医学,2014,29(3):262-265.
[3]尚红,王毓三,申子瑜.全国临床检验操作规程[M].第4版.北京:人民卫生出版社,2015.
[4]李勤,石佳,卢兴兵,等.XN-9000全自动血液细胞分析仪有核红细胞计数与手工方法验证的探讨[J].现代检验医学杂志,2017,32(1):124-127.
[5]陈建霞,黄衍锋,张旭.XE-5000全自动血细胞分析仪检测白细胞分类与人工镜检结果对比分析[J].中国当代医药,2015(12):124-126.
[6]顾梅秀,孟志民,唐文佳,等.SYSMEX XN-9000全自动血液分析仪有核红细胞镜检规则的建立与评价[J].检验医学,2017,32(6).
[2]王也飞,周怡,丁磊,等.SYSMEX XN-1000全自动血液分析仪计数有核红细胞的准确性评价[J].检验医学,2014,29(3):262-265.
[3]尚红,王毓三,申子瑜.全国临床检验操作规程[M].第4版.北京:人民卫生出版社,2015.
[4]李勤,石佳,卢兴兵,等.XN-9000全自动血液细胞分析仪有核红细胞计数与手工方法验证的探讨[J].现代检验医学杂志,2017,32(1):124-127.
[5]陈建霞,黄衍锋,张旭.XE-5000全自动血细胞分析仪检测白细胞分类与人工镜检结果对比分析[J].中国当代医药,2015(12):124-126.
[6]顾梅秀,孟志民,唐文佳,等.SYSMEX XN-9000全自动血液分析仪有核红细胞镜检规则的建立与评价[J].检验医学,2017,32(6).