通窍活血汤对大鼠脑缺血再灌注损伤后血清中TNF-α及IL-6水平的影响
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摘要
目的:观察通窍活血汤对大鼠脑缺血再灌注损伤后血清中肿瘤坏死因子-α(Tumor Necrosis Factor-α,TNF-α)、白介素-6 (Interleukin-6,IL-6)水平的影响。方法:SD雄性大鼠30只,随机分为假手术组、模型组及通窍活血汤低、中、高剂量组5组。采用线栓法建立大鼠脑缺血再灌注损伤模型,于缺血再灌注24 h后对大鼠进行神经功能损伤评分;酶联免疫吸附剂测定法(ELISA)检测各组血清中TNF-α、IL-6水平。结果:通窍活血汤高、中剂量组可明显减轻大鼠脑缺血再灌注后神经功能损伤和降低血清TNF-α和IL-6的水平。结论:通窍活血汤能够降低大鼠脑缺血再灌注损伤时炎症细胞因子的水平,从而减轻脑缺血再灌注损伤的程度。
Objective: To investigate the effect of Tongqiao-Huoxue Decoctionr on tumor necrosis factor-α(TNF-α) and interleukin-6( IL-6) after cerebral ischemia reperfusion injury in rat. Methods: Thirty healthy male SD rats were randomly divided into 5 groups: the sham operation group,the model group,Tongqiao-Huoxue Decoction groups of low,middle and high dose groups. Thread block method was used to establish the cerebral ischemia reperfusion injury model in rats. After the reperfusion for 24 h,efforts were made to give neurological score; detect TNF-α and IL-6 content in serum by the method of enzyme-linked immunosorbent assay(ELISA). Results: Tongqiao-Huoxue Decoction could greatly improved the neurological function and reduce TNF-α and IL-6 contents. Conclusion: Tongqiao-Huoxue Decoction can decrease proinflammatory cytokine levels after cerebral ischemia reperfusion injury in rats,reduce inflammatory reaction to lower intensity of ischemia reperfusion injury.
Objective: To investigate the effect of Tongqiao-Huoxue Decoctionr on tumor necrosis factor-α(TNF-α) and interleukin-6( IL-6) after cerebral ischemia reperfusion injury in rat. Methods: Thirty healthy male SD rats were randomly divided into 5 groups: the sham operation group,the model group,Tongqiao-Huoxue Decoction groups of low,middle and high dose groups. Thread block method was used to establish the cerebral ischemia reperfusion injury model in rats. After the reperfusion for 24 h,efforts were made to give neurological score; detect TNF-α and IL-6 content in serum by the method of enzyme-linked immunosorbent assay(ELISA). Results: Tongqiao-Huoxue Decoction could greatly improved the neurological function and reduce TNF-α and IL-6 contents. Conclusion: Tongqiao-Huoxue Decoction can decrease proinflammatory cytokine levels after cerebral ischemia reperfusion injury in rats,reduce inflammatory reaction to lower intensity of ischemia reperfusion injury.
引文
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[12] ZAREMBA J,LOSY J. Early tnf-alpha levels correlate with ischaemic stroke severity[J]. Acta Neurol Scand,2001,104(5):288-295.
[13]段乐,白利群,田苗苗,等.炎症反应在脑缺血中作用机制研究进展[J].中华实用诊断与治疗杂志,2013,27(9):837-839.
[14] JEAN PK,AMOS OA,OLALEKAN OE,et al. Therapeutic potential of plantextracts and phytochemicals against brain ischemia-reperfusion injury:areview[J].Nat Prod J,2016,6:250-284.
[15] ABBAS G,SALMAN A,RAHMAN SU,et al. Aging mechanisms:linking oxidative stress,obesity and inflammation[J]. Matrix Sci Med,2017,1(1):1-33.
[16]相鲁闽.《医林改错》与活血化瘀三汤方[J].河南中医,2014,34(10):1891.
[17]朱云中,张高炼,韦可聪,等.活血化瘀法对重型颅脑外伤术后患者血液流变学及神经功能的影响[J].现代中西医结合杂志,2015,24(10):1046-1048,1051.
[18]刘志华.通窍活血汤对脑梗死患者的疗效及血流变指标的影响分析[J].中医临床研究,2016,8(6):50-51.
[2] SANDERSON TH,REYNOLDS CA,KUMAR R,et al.Molecular mechanisms of ischemia-reperfusion injury in brain:Pivotal role of the mitochondrial membrane potential in reactive oxygen species generation[J]. Mol Neurobiol,2013,47(1):9-23.
[3] CAI F,LI CR,WU JL,et al. Theaflavin ameliorates cerebral ischemia-reperfusion injury in rats through its anti-inflammatory effect and modulation of STAT-1[J].Mediators Inflamm,2006(5):30490.
[4] AMANTEA D,NAPPI G,BERNARDI G,et al. Post-ischemia brain damage:Pathophysiology and role of inflammatory mediators[J]. FEBS J,2009,276(1):13-26.
[5]安聪,王学敏,黄宗轩,等.通窍活血汤水提取物对重型颅脑损伤大鼠血清白介素-6,白介素-10水平的影响[J].广西医学,2017,39(4):501-503.
[6]卢昌均,顿玲露,刘国成,等.通窍活血汤对脑缺血-再灌注大鼠基质细胞衍生因子-1的调控作用[J].医药导报,2016,35(1):44-46.
[7] SANDERSON TH,REYNOLDS CA,KUMAR R,et al.Molecular mechanisms of ischemia-reperfusion injury in brain:pivotal role of the mitochondrial membrane potentialin reactive oxygen species generation[J]. Mol Neurobiol,2013,47(1):9-23.
[8] ISHIBASHI N,PROKOPENKO O,REUHL KR,et al.Inflammatory response and glutathione peroxidase in a model of stroke[J]. J Immunol,2002,168(4):1926-1933.
[9] FRANGOGIANNIS NG. Chemokines in ischemia and reperfusion[J]. Thromb Haemost, 2007, 97(5):738-747.
[10] YAIDIKAR L AND THAKUR S. Punicalagin attenuated cerebral ischemia-reperfusion insult via inhibition of proinflammatory cytokines,up-egulation of Bcl-2,downregulation of Bax, and caspase-3[J]. Mol Cell Biochem,2015,402:141-148.
[11] HAILE WB,WU J,ECHEVERRY R,et al. Tissuetype plasminogen activator has a neuroprotective effect in the ischemic brain mediated by neuronal TNF-α[J]. J Cereb Blood Flow Metab,2012,32(1):57-69.
[12] ZAREMBA J,LOSY J. Early tnf-alpha levels correlate with ischaemic stroke severity[J]. Acta Neurol Scand,2001,104(5):288-295.
[13]段乐,白利群,田苗苗,等.炎症反应在脑缺血中作用机制研究进展[J].中华实用诊断与治疗杂志,2013,27(9):837-839.
[14] JEAN PK,AMOS OA,OLALEKAN OE,et al. Therapeutic potential of plantextracts and phytochemicals against brain ischemia-reperfusion injury:areview[J].Nat Prod J,2016,6:250-284.
[15] ABBAS G,SALMAN A,RAHMAN SU,et al. Aging mechanisms:linking oxidative stress,obesity and inflammation[J]. Matrix Sci Med,2017,1(1):1-33.
[16]相鲁闽.《医林改错》与活血化瘀三汤方[J].河南中医,2014,34(10):1891.
[17]朱云中,张高炼,韦可聪,等.活血化瘀法对重型颅脑外伤术后患者血液流变学及神经功能的影响[J].现代中西医结合杂志,2015,24(10):1046-1048,1051.
[18]刘志华.通窍活血汤对脑梗死患者的疗效及血流变指标的影响分析[J].中医临床研究,2016,8(6):50-51.