盐胁迫对杨树和紫丁香叶片叶绿素荧光特性的影响
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摘要
【目的】研究NaCl不同浓度胁迫处理对杨树和紫丁香叶片叶绿素荧光特性的影响。【方法】采用0(对照),200,400,600,800mmol/L NaCl溶液处理杨树和紫丁香叶片,研究两者在盐胁迫条件下叶绿素荧光参数的变化。【结果】随着NaCl处理浓度的增大,杨树和紫丁香叶片的PSⅡ最大光能转化效率(Fv/Fm)、PSⅡ有效光化学量子效率(Fv′/Fm′)、非循环光合电子传递速率(ETR)、PSⅡ实际光能转化效率(ΦPSⅡ)以及光化学猝灭系数(qp)均呈下降趋势。比较而言,杨树Fv/Fm下降并不显著,且在非胁迫环境或较低盐胁迫条件下,杨树叶片的Fv′/Fm′、ETR、ΦPSⅡ以及qp值高于紫丁香叶片,而非光化学猝灭系数(NPQ)值低于紫丁香叶片。【结论】在非胁迫环境或较低盐胁迫条件下,杨树叶片光合作用的光化学性能优于紫丁香叶片。
【Objective】 Effects of NaCl solutions with different concentrations on the chlorophyll fluorescence parameters of poplar and lilac leaves were studied.【Method】 Poplar and lilac leaves living in same circumstance were cultured with 0(control),200,400,600,800 mmol/L NaCl to investigate the variation of chlorophyll fluorescence parameters.【Result】 The results showed that the maximal photochemical efficiency of PSⅡ(Fv/Fm),the photochemical efficiency of PSⅡ in light(Fv′/Fm′),the intrinsic PSⅡ efficiency(ETR),the actual photochemical efficiency of PSⅡ in the light(ΦPSⅡ),and the photochemical quenching coefficient(qp) in poplar and lilac leaves decreased as the increase of NaCl concentrations.Compared with lilac leaves,poplar leaves had more stable level of Fv/Fm and higher values of Fv′/Fm′,ETR,ΦPSⅡ,and qp.Moreover,non-photochemical quenching coefficient(NPQ) of poplar leaves was lower than that of lilac leaves.【Conclusion】 These results indicated that under non-stress or low-salt stress,poplar leaves had superior photochemical properties than lilac leaves.
【Objective】 Effects of NaCl solutions with different concentrations on the chlorophyll fluorescence parameters of poplar and lilac leaves were studied.【Method】 Poplar and lilac leaves living in same circumstance were cultured with 0(control),200,400,600,800 mmol/L NaCl to investigate the variation of chlorophyll fluorescence parameters.【Result】 The results showed that the maximal photochemical efficiency of PSⅡ(Fv/Fm),the photochemical efficiency of PSⅡ in light(Fv′/Fm′),the intrinsic PSⅡ efficiency(ETR),the actual photochemical efficiency of PSⅡ in the light(ΦPSⅡ),and the photochemical quenching coefficient(qp) in poplar and lilac leaves decreased as the increase of NaCl concentrations.Compared with lilac leaves,poplar leaves had more stable level of Fv/Fm and higher values of Fv′/Fm′,ETR,ΦPSⅡ,and qp.Moreover,non-photochemical quenching coefficient(NPQ) of poplar leaves was lower than that of lilac leaves.【Conclusion】 These results indicated that under non-stress or low-salt stress,poplar leaves had superior photochemical properties than lilac leaves.
引文
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[9]冯建灿,胡秀丽,毛训甲.叶绿素荧光动力学在研究植物逆境生理中的应用[J].经济林研究,2012,20(4):14-18.Feng J C,Hu X L,Mao X J.Application of chlorophy fluores-cencedynamics to plant physiology in adverse circumstance[J].Economic Forest Researches,2012,20(4):14-18.(in Chi-nese)
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[11]万雪琴,夏新莉,尹伟伦,等.不同杨树无性系扦插苗水分利用效率的差异及其生理机制[J].林业科学,2006,42(5):133-137.Wan X Q,Xia X L,Yin W L,et al.Water use efficiency differ-ence among four poplar clones and its physiological mecha-nism[J].Scientia Silvae Sinicae,2006,42(5):133-137.(inChinese)
[12]张勇,张守攻,齐力旺,等.杨树:林木基因组学研究的模式物种[J].植物学通报,2006,23(3):286-293.Zhang Y,Zhang S G,Qi L W,et al.Poplar as a model for for-est tree in genome research[J].Chinese Bulletin of Botany,2006,23(3):286-293.(in Chinese)
[13]李永吉,吕邵娃,王艳宏,等.丁香叶药用研究进展[J].中医药信息,2003,20(1):22-23.Li Y J,LüS W,Wang Y H,et al.Clove leaf medicinal re-search progress[J].Information on Traditional Chinese Med-icine,2003,20(1):22-23.(in Chinese)
[14]陈少良,李金克,毕望富,等.盐胁迫条件下杨树盐分与甜菜碱及糖类物质变化[J].植物学通报,2001,18(5):587-596.Chen S L,Li J K,Bi W F,et al.Genotypic variation in accu-mulation of salt ions,betaine and sugars in poplar under con-ditions of salt stress[J].Chinese Bulletin of Botany,2001,18(5):587-596.(in Chinese)
[15]杨忠顺.五种木本植物颉颃盐逆境演化结构比较研究[D].长春:东北师范大学,2004.Yang Z S.Comparative study of the evolving structure on saltresistance in five kinds of xylophyta[J].Changchun:North-east Normal University,2004.(in Chinese)
[16]陈建明,俞晓平,程家安.叶绿素荧光动力学及其在植物抗逆生理研究中的应用[J].浙江农业学报,2006,18(1):51-55.Chen J M,Yu X P,Cheng J A.The application of chlorophyllfluorescence kinetics in the study of physiological responses ofplants to environmental stresses[J].Acta Agriculturae Zhe-jiangensis,2006,18(1):51-55.(in Chinese)
[17]Hong S S,Xu D Q.Light induced increase in initial fluores-cence parameters to strong light between wheat and soybeanleaves[J].Chinese Science Bulletin,1997,42:684-688.
[18]孙宪芝,郑成淑,王秀峰.高温胁迫对切花菊“神马”光合作用与叶绿素荧光的影响[J].应用生态学报,2008,19(10):2149-2154.Sun X Z,Zheng C S,Wang X F.Effects of high temperaturestress on photosynthesis and chlorophyll fluorescence of cutflower chrysanthemum(Dendranthema grandiflora‘Jinba’)[J].Chinese Journal of Applied Ecology,2008,19(10):2149-2154.(in Chinese)
[19]周朝彬,宋于洋,王炳举,等.干旱胁迫对胡杨光合和叶绿素荧光参数的影响[J].西北林学院学报,2009,24(4):5-9.Zhou C B,Song Y Y,Wang B J,et al.Effects of drought stresson photosynthesis and chlorophyll fluorescence parameters ofPopulus euphratica[J].Journal of Northwest Forestry Uni-versity,2009,24(4):5-9.(in Chinese)
[20]张守仁.叶绿素荧光动力学参数的意义及讨论[J].植物学通报,1999,16(4):444-448.Zhang S R.A discussion on chlorophyll fluorescence kineticsparameters and their significance[J].Chinese Bulletin of Bot-any,1999,16(4):444-448.(in Chinese)
[21]Genty B E,Briantais J M,Baker N R.The relationship betwe-en the quantum yield of photosynthetic electron transport andquenching of chlorophyll fluorescence[J].Biochem BiophysActa,1989,990:87-92.
[22]温国胜,田海涛,张明如,等.叶绿素荧光分析技术在林木培育中的应用[J].应用生态学报,2006,17(10):1973-1977.Wen G S,Tian H T,Zhang M R,et al.Application of chloro-phyll fluorescence analysis in forest tree cultivation[J].Chi-nese Journal of Applied Ecology,2006,17(10):1973-1977.(in Chinese)
[2]符秀梅,朱红林,李小靖,等.盐胁迫对水稻幼苗生长及生理生化的影响[J].广东农业科学,2010(4):19-21.Fu X M,Zhu H L,Li X J,et al.Effects of NaCl stress on thegrowth and physio-biochemical characteristics of rice seedlings[J].Guangdong Agricultural Sciences,2010(4):19-21.(in Chi-nese)
[3]杨颖丽,杨宁,王菜,等.盐胁迫对小麦幼苗生理指标的影响[J].兰州大学学报:自然科学版,2007,43(2):29-34.Yang Y L,Yang N,Wang C,et al.Effect of salinity on thephysiological characteristics in two wheat cultivars[J].Journalof Lanzhou University:Natural Sciences,2007,43(2):29-34.(in Chinese)
[4]汪贵斌,曹福亮,游庆方,等.盐胁迫对4树种叶片中K+和Na+的影响及其耐盐能力的评价[J].植物资源与环境学报,2001,10(1):30-34.Wang G B,Cao F L,You Q F,et al.Effects of salt stress onconcentrations of Na+,K+and Na+//K+in the leaves of fourtree species and evaluation of salt tolerance[J].Journal ofPlant Resources Environment,2001,10(1):30-34.(in Chinese)
[5]孟凡娟,王建中,黄凤兰,等.盐胁迫对两种刺槐叶肉细胞超微结构的影响[J].北京林业大学学报,2010,32(4):97-102.Meng F J,Wang J Z,Huang F L,et al.Ultrastructure of meso-phyll cells in two Robinia pseudoacacia hybrids under NaClstress[J].Journal of Beijing Forestry University,2010,32(4):97-102.(in Chinese)
[6]王守超,王得祥,彭少兵,等.盐胁迫对木本滨藜植物细胞膜透性及生理特性的影响[J].干旱地区农业研究,2007(4):281-285.Wang S C,Wang D X,Peng S B,et al.Effects of salt stress oncell membrane permeability and physiological property inwoody saltbush[J].Agricultural Research in the Arid,2007(4):281-285.(in Chinese)
[7]严俊鑫,刘晓东,张晓娇,等.6种丁香的光合特性[J].东北林业大学学报,2008,36(7):23-41.Yan J X,Liu X D,Zhang X J,et al.Photosynthetic characteris-tics of six species of Syinga[J].Journal of Northeast ForestryUniversity,2008,36(7):23-41.(in Chinese)
[8]蔡丽敏,董丽.叶绿素荧光技术在园林植物中的应用[J].北方园艺,2007(3):75-77.Cai L M,Dong L.Application of chlorophyll fluorescence tech-nique in garden plants[J].Northern Horticulture,2007(3):75-77.(in Chinese)
[9]冯建灿,胡秀丽,毛训甲.叶绿素荧光动力学在研究植物逆境生理中的应用[J].经济林研究,2012,20(4):14-18.Feng J C,Hu X L,Mao X J.Application of chlorophy fluores-cencedynamics to plant physiology in adverse circumstance[J].Economic Forest Researches,2012,20(4):14-18.(in Chi-nese)
[10]天锡,陈章水.中国杨树集约栽培[M].北京:中国科学技术出版社,1994.Tian X,Chen Z S.Intensive cultivation of poplar in China[M].Beijing:China Science and Technology Press,1994.(inChinese)
[11]万雪琴,夏新莉,尹伟伦,等.不同杨树无性系扦插苗水分利用效率的差异及其生理机制[J].林业科学,2006,42(5):133-137.Wan X Q,Xia X L,Yin W L,et al.Water use efficiency differ-ence among four poplar clones and its physiological mecha-nism[J].Scientia Silvae Sinicae,2006,42(5):133-137.(inChinese)
[12]张勇,张守攻,齐力旺,等.杨树:林木基因组学研究的模式物种[J].植物学通报,2006,23(3):286-293.Zhang Y,Zhang S G,Qi L W,et al.Poplar as a model for for-est tree in genome research[J].Chinese Bulletin of Botany,2006,23(3):286-293.(in Chinese)
[13]李永吉,吕邵娃,王艳宏,等.丁香叶药用研究进展[J].中医药信息,2003,20(1):22-23.Li Y J,LüS W,Wang Y H,et al.Clove leaf medicinal re-search progress[J].Information on Traditional Chinese Med-icine,2003,20(1):22-23.(in Chinese)
[14]陈少良,李金克,毕望富,等.盐胁迫条件下杨树盐分与甜菜碱及糖类物质变化[J].植物学通报,2001,18(5):587-596.Chen S L,Li J K,Bi W F,et al.Genotypic variation in accu-mulation of salt ions,betaine and sugars in poplar under con-ditions of salt stress[J].Chinese Bulletin of Botany,2001,18(5):587-596.(in Chinese)
[15]杨忠顺.五种木本植物颉颃盐逆境演化结构比较研究[D].长春:东北师范大学,2004.Yang Z S.Comparative study of the evolving structure on saltresistance in five kinds of xylophyta[J].Changchun:North-east Normal University,2004.(in Chinese)
[16]陈建明,俞晓平,程家安.叶绿素荧光动力学及其在植物抗逆生理研究中的应用[J].浙江农业学报,2006,18(1):51-55.Chen J M,Yu X P,Cheng J A.The application of chlorophyllfluorescence kinetics in the study of physiological responses ofplants to environmental stresses[J].Acta Agriculturae Zhe-jiangensis,2006,18(1):51-55.(in Chinese)
[17]Hong S S,Xu D Q.Light induced increase in initial fluores-cence parameters to strong light between wheat and soybeanleaves[J].Chinese Science Bulletin,1997,42:684-688.
[18]孙宪芝,郑成淑,王秀峰.高温胁迫对切花菊“神马”光合作用与叶绿素荧光的影响[J].应用生态学报,2008,19(10):2149-2154.Sun X Z,Zheng C S,Wang X F.Effects of high temperaturestress on photosynthesis and chlorophyll fluorescence of cutflower chrysanthemum(Dendranthema grandiflora‘Jinba’)[J].Chinese Journal of Applied Ecology,2008,19(10):2149-2154.(in Chinese)
[19]周朝彬,宋于洋,王炳举,等.干旱胁迫对胡杨光合和叶绿素荧光参数的影响[J].西北林学院学报,2009,24(4):5-9.Zhou C B,Song Y Y,Wang B J,et al.Effects of drought stresson photosynthesis and chlorophyll fluorescence parameters ofPopulus euphratica[J].Journal of Northwest Forestry Uni-versity,2009,24(4):5-9.(in Chinese)
[20]张守仁.叶绿素荧光动力学参数的意义及讨论[J].植物学通报,1999,16(4):444-448.Zhang S R.A discussion on chlorophyll fluorescence kineticsparameters and their significance[J].Chinese Bulletin of Bot-any,1999,16(4):444-448.(in Chinese)
[21]Genty B E,Briantais J M,Baker N R.The relationship betwe-en the quantum yield of photosynthetic electron transport andquenching of chlorophyll fluorescence[J].Biochem BiophysActa,1989,990:87-92.
[22]温国胜,田海涛,张明如,等.叶绿素荧光分析技术在林木培育中的应用[J].应用生态学报,2006,17(10):1973-1977.Wen G S,Tian H T,Zhang M R,et al.Application of chloro-phyll fluorescence analysis in forest tree cultivation[J].Chi-nese Journal of Applied Ecology,2006,17(10):1973-1977.(in Chinese)