NaCl胁迫下不同抗性野生大豆体内Na~+,K~+,Cl~-浓度比较分析
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摘要
选取盐敏感型辉南野生大豆和耐盐型通榆野生大豆植物为材料,在5片复叶时期进行了10d的NaCl胁迫处理,测定了根、茎、叶中Na+,K+,Cl-的浓度,以探讨NaCl胁迫下野生大豆体内离子平衡的特点.结果表明:盐敏感型和耐盐型野生大豆植物在NaCl胁迫下,体内Na+和Cl-含量随胁迫浓度增加呈上升趋势,K+含量呈下降趋势.高盐胁迫下,除根部的K+外,其余各部位的Na+,K+,Cl-含量均表现为耐盐型野生大豆低于盐敏感型野生大豆.实验结果证明,通榆野生大豆具有颉颃盐逆境的生理特征和较好的适应性.
The experimental materials are Huinan types of wild soybean(salt sensitive)and Tongyu types of wild soybean(salt resistant),during the period of 5compound leaves,NaCl stress treatment is applied with 10 days.The content of Na+,K+,Cl-in roots,stems,and leaves were determined to investigate the characteristics of the ion balance in wild soybean body under the stress of NaCl.The results show that,in two kinds of wild soybean plants,Na+and Cl-content showed an increasing trend with the increase of stress concentration,whereas K+content decreased under the stress of NaCl.The concentration of Na+,K+and Cl-showed that each part of salt resistant wild soybean were generally lower than the ion concentration in salt sensitive wild soybean,except K+in the roots,under the high salt stress.The following points through the analysis,Tongyu types of wild soybean has better adaptability to saline land.
The experimental materials are Huinan types of wild soybean(salt sensitive)and Tongyu types of wild soybean(salt resistant),during the period of 5compound leaves,NaCl stress treatment is applied with 10 days.The content of Na+,K+,Cl-in roots,stems,and leaves were determined to investigate the characteristics of the ion balance in wild soybean body under the stress of NaCl.The results show that,in two kinds of wild soybean plants,Na+and Cl-content showed an increasing trend with the increase of stress concentration,whereas K+content decreased under the stress of NaCl.The concentration of Na+,K+and Cl-showed that each part of salt resistant wild soybean were generally lower than the ion concentration in salt sensitive wild soybean,except K+in the roots,under the high salt stress.The following points through the analysis,Tongyu types of wild soybean has better adaptability to saline land.
引文
[1]李福山.中国野生大豆资源的地理分布及生态分化研究[J].中国农业科学,1993,26(2):47-55.
[2]李孟良,马双雷.五河野生大豆生物学特性初步研究[J].安徽科技学院学报,2009,23(6):15-18.
[3]陆静梅,刘友良,胡波,等.中国野生大豆盐腺的发现[J].科学通报,1998,19:2074-2078.
[4]李伟强,刘小京,赵可夫,等.NaCl胁迫下3种盐生植物生长发育及离子在不同器官分布特性研究[J].中国生态农业学报,2006,16(2):49-52.
[5]MARK T,ROMOLA D.Na+tolerance and Na+transport in higher plants[J].Annuals of Botany,2003,91(5):503-527.
[6]杨劲松.中国盐渍土研究的发展历程与展望[J].土壤学报,2008,45(5):837-845.
[7]GLENN E P,BROWN J J,BLUMWALD EDUARDO.Salt tolerance and crop potential of halophytes[J].Critical Review in Plant Science,1999,18(2):227-255.
[8]MUNNS R.Comparative physiology of salt and water stress[J].Plant,Cell and Environment,2002,25:239-250.
[9]MAATHUIS F J M,AMTMANN A.K+nutrition and Na+toxicity:the basis of cellular K+/Na+ratios[J].Annals of Botany,1999,84:123-133.
[10]MORGAN J M.Osmotic adjustment in the spikelets and leaves of wheat[J].Journal of Experimental Botany,1980,31(2):655-665.
[11]ESSA T A.Effect of salinity stress on growth and nutrient composition of three soybean(Glycine max L.Merrill)cultivars[J].J Agronomy&Crop Science,2002,188:86-93.
[12]KAYA C,HIGGS D,SAKAR E.Response of two leaf vegetables grown at high salinity to supplementary potassium and phosphorus during different growth stages[J].Journal of Plant Nutrition,2002,25:2663-2676.
[13]GREEWAY H,MUNNS R.Mechanism of salt tolerance in nonhalophytes[J].Annual Review of Plant Physiology,1980,31:149-190.
[14]杨敏生,李艳华,梁海永,等.盐胁迫下白杨无性系苗木体内离子分配及比较[J].生态学报,2003,23(2):271-277.
[15]HARVEY D M R.Effects of different external potassium concentrations on potassium concentrations in the cytoplasm of maize root cortical cells[J].Journal of Plant Physiology,1988,133(6):768-769.
[16]SHATERIAN J,WATERER D,JONG H,et al.Differential stress responses to NaCl salt application in early-and latematuring diploid potato(Solanumsp.)clones[J].Environmental and Experimental Botany,2005,54:202-212.
[17]CHARTZOULAKIS K,KLAPAKI G.Response of two greenhouse pepper hybrids to NaCl salinity during different growth stages[J].Scientia Horticulturae,2000,86:247-260.
[18]SIVRITEPE N,SIVRITEPE H O,ERIS A.The effects of NaCl priming on salt tolerance in melon seedlings grown under saline conditions[J].Scientia Horticulturae,2003,97:229-237.
[19]NETONDO G W,ONYANGO J C,BECK E.Sorghum and salinity:I.response of growth,water relations,and ion accumulation to NaCl salinity[J].Crop Science,2004,44(3):797-805.
[20]YAN Q,WANG,JIANG X N,et al.The changes of ion absorbing and distribution in different organ and cell of Robinia pseudoacacia seedlings for salt stress[J].Journal Beijing Forestry University Chinese Edition,2001,23(1):18-23.
[21]SHI D C,SHENG Y M,ZHAO K F.Stress effects of mixed salts with various salinities on the seedlings of Aneurolepidium Chinese[J].Acta Botanica Sinica,1998,40(12):1136-1142.
[22]YIN L J,SHI D C,WANG P.Growth adaptability and salt tolerance osmoregulation of Aneurolepidium Chinese grown on saline grassland[J].Acta Botanica Sinica,1993,35(8):619-625.
[23]LUCHLI A,LTTGE U.Salinity:environment-plants-molecules[M].Boston:Boston Kluwer Academic Publishers,2002:21-23.
[24]RICKAUER M,TANNER W.Effects of Ca2+on amino acid transport and accumulation in roots of Phaseolusvulgaris[J].Plant Physiol,1986,82:41-46.
[25]ABEL G H,MACKENZIE A J.Salt tolerance of soybean varieties(Glycine max(L).Merrill)during germination and later growth[J].Crop Sci,1964(4):157-161.
[26]邵桂花,万超文,李舒凡.大豆萌发期耐盐生理初步研究[J].作物杂志,1994(6):25-27.
[27]刘玉杰,王宝增.不同品种大豆耐盐性的比较研究[J].安徽农业科学,2007,35(15):4462-4464.
[28]邵桂花,常汝镇,陈一舞.大豆耐盐性研究进展[J].大豆科学,1993,12(3):244-248.
[29]万超文,邵桂花,陈一舞,等.中国盐胁迫下大豆耐盐性与籽粒化学质量的关系[J].中国油料作物学报,2002,24(2):67-72.
[30]HU Z A,WANG H X.Salt tolerance of wild soybean(Glycine soja)in natural populations evaluated by a new method[J].Soybean genetics Newsl,1997,24:79-80.
[31]马淑时,王伟.大豆品种资源的抗盐碱性研究[J].吉林农业科学,1994(4):69-71.
[32]寇贺,曹敏建,那桂秋.大豆种子萌发期耐盐性综合鉴定指标初探[J].杂粮作物,2007,27(5):352-354.
[2]李孟良,马双雷.五河野生大豆生物学特性初步研究[J].安徽科技学院学报,2009,23(6):15-18.
[3]陆静梅,刘友良,胡波,等.中国野生大豆盐腺的发现[J].科学通报,1998,19:2074-2078.
[4]李伟强,刘小京,赵可夫,等.NaCl胁迫下3种盐生植物生长发育及离子在不同器官分布特性研究[J].中国生态农业学报,2006,16(2):49-52.
[5]MARK T,ROMOLA D.Na+tolerance and Na+transport in higher plants[J].Annuals of Botany,2003,91(5):503-527.
[6]杨劲松.中国盐渍土研究的发展历程与展望[J].土壤学报,2008,45(5):837-845.
[7]GLENN E P,BROWN J J,BLUMWALD EDUARDO.Salt tolerance and crop potential of halophytes[J].Critical Review in Plant Science,1999,18(2):227-255.
[8]MUNNS R.Comparative physiology of salt and water stress[J].Plant,Cell and Environment,2002,25:239-250.
[9]MAATHUIS F J M,AMTMANN A.K+nutrition and Na+toxicity:the basis of cellular K+/Na+ratios[J].Annals of Botany,1999,84:123-133.
[10]MORGAN J M.Osmotic adjustment in the spikelets and leaves of wheat[J].Journal of Experimental Botany,1980,31(2):655-665.
[11]ESSA T A.Effect of salinity stress on growth and nutrient composition of three soybean(Glycine max L.Merrill)cultivars[J].J Agronomy&Crop Science,2002,188:86-93.
[12]KAYA C,HIGGS D,SAKAR E.Response of two leaf vegetables grown at high salinity to supplementary potassium and phosphorus during different growth stages[J].Journal of Plant Nutrition,2002,25:2663-2676.
[13]GREEWAY H,MUNNS R.Mechanism of salt tolerance in nonhalophytes[J].Annual Review of Plant Physiology,1980,31:149-190.
[14]杨敏生,李艳华,梁海永,等.盐胁迫下白杨无性系苗木体内离子分配及比较[J].生态学报,2003,23(2):271-277.
[15]HARVEY D M R.Effects of different external potassium concentrations on potassium concentrations in the cytoplasm of maize root cortical cells[J].Journal of Plant Physiology,1988,133(6):768-769.
[16]SHATERIAN J,WATERER D,JONG H,et al.Differential stress responses to NaCl salt application in early-and latematuring diploid potato(Solanumsp.)clones[J].Environmental and Experimental Botany,2005,54:202-212.
[17]CHARTZOULAKIS K,KLAPAKI G.Response of two greenhouse pepper hybrids to NaCl salinity during different growth stages[J].Scientia Horticulturae,2000,86:247-260.
[18]SIVRITEPE N,SIVRITEPE H O,ERIS A.The effects of NaCl priming on salt tolerance in melon seedlings grown under saline conditions[J].Scientia Horticulturae,2003,97:229-237.
[19]NETONDO G W,ONYANGO J C,BECK E.Sorghum and salinity:I.response of growth,water relations,and ion accumulation to NaCl salinity[J].Crop Science,2004,44(3):797-805.
[20]YAN Q,WANG,JIANG X N,et al.The changes of ion absorbing and distribution in different organ and cell of Robinia pseudoacacia seedlings for salt stress[J].Journal Beijing Forestry University Chinese Edition,2001,23(1):18-23.
[21]SHI D C,SHENG Y M,ZHAO K F.Stress effects of mixed salts with various salinities on the seedlings of Aneurolepidium Chinese[J].Acta Botanica Sinica,1998,40(12):1136-1142.
[22]YIN L J,SHI D C,WANG P.Growth adaptability and salt tolerance osmoregulation of Aneurolepidium Chinese grown on saline grassland[J].Acta Botanica Sinica,1993,35(8):619-625.
[23]LUCHLI A,LTTGE U.Salinity:environment-plants-molecules[M].Boston:Boston Kluwer Academic Publishers,2002:21-23.
[24]RICKAUER M,TANNER W.Effects of Ca2+on amino acid transport and accumulation in roots of Phaseolusvulgaris[J].Plant Physiol,1986,82:41-46.
[25]ABEL G H,MACKENZIE A J.Salt tolerance of soybean varieties(Glycine max(L).Merrill)during germination and later growth[J].Crop Sci,1964(4):157-161.
[26]邵桂花,万超文,李舒凡.大豆萌发期耐盐生理初步研究[J].作物杂志,1994(6):25-27.
[27]刘玉杰,王宝增.不同品种大豆耐盐性的比较研究[J].安徽农业科学,2007,35(15):4462-4464.
[28]邵桂花,常汝镇,陈一舞.大豆耐盐性研究进展[J].大豆科学,1993,12(3):244-248.
[29]万超文,邵桂花,陈一舞,等.中国盐胁迫下大豆耐盐性与籽粒化学质量的关系[J].中国油料作物学报,2002,24(2):67-72.
[30]HU Z A,WANG H X.Salt tolerance of wild soybean(Glycine soja)in natural populations evaluated by a new method[J].Soybean genetics Newsl,1997,24:79-80.
[31]马淑时,王伟.大豆品种资源的抗盐碱性研究[J].吉林农业科学,1994(4):69-71.
[32]寇贺,曹敏建,那桂秋.大豆种子萌发期耐盐性综合鉴定指标初探[J].杂粮作物,2007,27(5):352-354.