不同生态环境下野生大豆根解剖结构演化研究
|
摘要
以不同生态环境下的野生大豆,即:野生大豆2013-001-01(盐生环境)和野生大豆2013-001-02(中生环境)为试材,基于结构植物学角度,应用石蜡切片和光学显微技术对供试的野生大豆根解剖结构进行比较研究,旨在为我国大豆属植物颉颃盐逆境研究提供解剖学依据。结果表明:2013-001-02根的维管柱为五原型,2013-001-01根的维管柱已经演化为四原型,相对于2013-001-02,2013-001-01根的皮层较完整,韧皮纤维发达且形成"韧皮屏障",次生木质部导管数量较多且存在侵填体,导管的傍管薄壁细胞较大且数量较多,射线径向长度相对较短。研究证明:野生大豆2013-001-01植物的根已经演化出颉颃盐逆境解剖结构。
In this study,the experimental Glycine materials were collected from different ecological environments: the wild soybean 2013-001-01( under the saline stress environment) and the wild soybean2013-001-02( under the suitable environment). This study used the paraffin section technology and the optical microscopy to compare their anatomical structure of the roots. The result of the experiment showed that unlike the wild soybean 2013-001-02 with vascular cylinder of pentarch,the wild soybean 2013-001-01 evolved into tetrarch of vascular cylinder from pentarch. The wild soybean 2013-001-01 had following characteristics compared with the wild soybean 2013-001-02: the cortical tissue was more complete,the developed phloem fiber become to "phloem fiber barrier",a large number of vessels and tylosis appeared in the secondary xylem,the paratracheal parenchyma cells of vessels were bigger and more,the length of the ray was shorter. In a word,the structure of antagonism to the saline stress appeared in the wild soybean2013-001-01.
In this study,the experimental Glycine materials were collected from different ecological environments: the wild soybean 2013-001-01( under the saline stress environment) and the wild soybean2013-001-02( under the suitable environment). This study used the paraffin section technology and the optical microscopy to compare their anatomical structure of the roots. The result of the experiment showed that unlike the wild soybean 2013-001-02 with vascular cylinder of pentarch,the wild soybean 2013-001-01 evolved into tetrarch of vascular cylinder from pentarch. The wild soybean 2013-001-01 had following characteristics compared with the wild soybean 2013-001-02: the cortical tissue was more complete,the developed phloem fiber become to "phloem fiber barrier",a large number of vessels and tylosis appeared in the secondary xylem,the paratracheal parenchyma cells of vessels were bigger and more,the length of the ray was shorter. In a word,the structure of antagonism to the saline stress appeared in the wild soybean2013-001-01.
引文
[1]杨劲松.中国盐渍土研究的发展历程与展望[J].土壤学报,2008,45(5):837-845.
[2]Abel G H,Mac Kenzie A J.Salt tolerance of soybean varieties(Glycine max L.Merrill)during germination and later growth[J].Crop Science,1964,4(2):157-161.
[3]邵桂花,万超文,李舒凡.大豆萌发期耐盐生理初步研究[J].作物杂志,1994(6):25-27.
[4]刘玉杰,王宝增.不同品种大豆耐盐性的比较研究[J].安徽农业科学,2007,35(15):4462-4464.
[5]邵桂花,常汝镇,陈一舞.大豆耐盐性研究进展[J].大豆科学,1993,12(3):244-248.
[6]万超文,邵桂花,陈一舞,等.盐胁迫下大豆耐盐性与籽粒化学品质的关系[J].中国油料作物学报,2002,24(2):67-72.
[7]庄炳昌.中国野生大豆研究二十年[J].吉林农业科学,1999(5):3-10.
[8]马晓萍,杨光宇,杨振宇,等.野生大豆在大豆育种中的应用[J].作物研究,2009(1):11-12.
[9]王志友,王昌陵,董丽杰,等.辽宁省野生大豆种质资源及利用现状[J].杂粮作物,2008(4):241-243.
[10]Zhong M,Hu Z A,Gresshoff P M.Search for molecular markers of salt tolerance of soybean by DNA amplification fingerprinting[J].Soybean Genetics Newsletter(USA),1997,4(24):81-82.
[11]Jiang B,Nan H,Gao Y,et al.Allelic combinations of soybean maturity loci E1,E2,E3 and E4 result in diversity of maturity and adaptation to different latitudes[J].Plos One,2014,9(8):e106042.
[12]李锦锦,王昉,张万科,等.发根农杆菌介导不同基因型大豆转化效率的筛选[J].河南农业科学,2012,41(5):37-41.
[13]刘大伟,陈立杰,段玉玺.大豆胞囊线虫胁迫下不同抗性大豆杂交后代根系蛋白质组分析[J].华北农学报,2013,28(5):29-33.
[14]任海红,任小俊,王英,等.外源DNA直接导入技术及在大豆育种中的应用[J].山西农业科学,2013,41(5):503-505,514.
[15]Durgaprasad K M R,Muthukumarsamy M,Panneerselvam R.Changes in protein metabolism induced by Na Cl salinity in soybean seedlings[J].Indian Journal of Plant Physiology,1996,1:98-101.
[16]El-Samad H M A,Shaddad M A K.Salt tolerance of soybean cultivars[J].Biologia Plantarum,1997,39(2):263-269.
[17]李煜昶,岳铭秀,董景华.烯效唑对大豆的增产作用[J].天津农业科学,1998,4(2):15-17.
[18]王勋陵.植物形态结构与环境[M].兰州:兰州大学出版社,1989.
[19]李正理.植物制片技术[M].北京:中国科学出版社,1987.
[20]陆时万,徐祥生,沈敏健.植物学[M].2版.北京:高等教育出版社,1991.
[21]Niu L,Lu J,Wu D,et al.Changes in the vascular cylinder of wild soybean roots under alkaline stress[J].Journal of Integrative Agriculture,2014,13(10):2164-2169.
[2]Abel G H,Mac Kenzie A J.Salt tolerance of soybean varieties(Glycine max L.Merrill)during germination and later growth[J].Crop Science,1964,4(2):157-161.
[3]邵桂花,万超文,李舒凡.大豆萌发期耐盐生理初步研究[J].作物杂志,1994(6):25-27.
[4]刘玉杰,王宝增.不同品种大豆耐盐性的比较研究[J].安徽农业科学,2007,35(15):4462-4464.
[5]邵桂花,常汝镇,陈一舞.大豆耐盐性研究进展[J].大豆科学,1993,12(3):244-248.
[6]万超文,邵桂花,陈一舞,等.盐胁迫下大豆耐盐性与籽粒化学品质的关系[J].中国油料作物学报,2002,24(2):67-72.
[7]庄炳昌.中国野生大豆研究二十年[J].吉林农业科学,1999(5):3-10.
[8]马晓萍,杨光宇,杨振宇,等.野生大豆在大豆育种中的应用[J].作物研究,2009(1):11-12.
[9]王志友,王昌陵,董丽杰,等.辽宁省野生大豆种质资源及利用现状[J].杂粮作物,2008(4):241-243.
[10]Zhong M,Hu Z A,Gresshoff P M.Search for molecular markers of salt tolerance of soybean by DNA amplification fingerprinting[J].Soybean Genetics Newsletter(USA),1997,4(24):81-82.
[11]Jiang B,Nan H,Gao Y,et al.Allelic combinations of soybean maturity loci E1,E2,E3 and E4 result in diversity of maturity and adaptation to different latitudes[J].Plos One,2014,9(8):e106042.
[12]李锦锦,王昉,张万科,等.发根农杆菌介导不同基因型大豆转化效率的筛选[J].河南农业科学,2012,41(5):37-41.
[13]刘大伟,陈立杰,段玉玺.大豆胞囊线虫胁迫下不同抗性大豆杂交后代根系蛋白质组分析[J].华北农学报,2013,28(5):29-33.
[14]任海红,任小俊,王英,等.外源DNA直接导入技术及在大豆育种中的应用[J].山西农业科学,2013,41(5):503-505,514.
[15]Durgaprasad K M R,Muthukumarsamy M,Panneerselvam R.Changes in protein metabolism induced by Na Cl salinity in soybean seedlings[J].Indian Journal of Plant Physiology,1996,1:98-101.
[16]El-Samad H M A,Shaddad M A K.Salt tolerance of soybean cultivars[J].Biologia Plantarum,1997,39(2):263-269.
[17]李煜昶,岳铭秀,董景华.烯效唑对大豆的增产作用[J].天津农业科学,1998,4(2):15-17.
[18]王勋陵.植物形态结构与环境[M].兰州:兰州大学出版社,1989.
[19]李正理.植物制片技术[M].北京:中国科学出版社,1987.
[20]陆时万,徐祥生,沈敏健.植物学[M].2版.北京:高等教育出版社,1991.
[21]Niu L,Lu J,Wu D,et al.Changes in the vascular cylinder of wild soybean roots under alkaline stress[J].Journal of Integrative Agriculture,2014,13(10):2164-2169.