棕榈蓟马2种杀虫剂抗感种群间多种酶活性的比较
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摘要
为明确乙基多杀菌素和吡虫啉对棕榈蓟马的抗性风险,采用叶管药膜法测定了棕榈蓟马种群对乙基多杀菌素和吡虫啉的抗性倍数,比较了其抗性种群和敏感种群之间羧酸酯酶(CarE)、谷胱甘肽-S-转移酶(GST)、乙酰胆碱酯酶(AchE)3种酶的活性和细胞色素P450、细胞色素b5的含量变化。结果表明,棕榈蓟马种群对吡虫啉产生了中等水平的抗性(抗性倍数为12.73),对乙基多杀菌素产生了低水平的抗性(抗性倍数为9.53)。除棕榈蓟马吡虫啉抗性种群(BK)的AchE活性下降(0.58倍)外,乙基多杀菌素抗性种群(DK)的AchE活性则提高为1.31倍;DK和BK的CarE活性分别提高为敏感种群的1.46、1.79倍,GST活性分别提高为1.40倍和1.59倍,细胞色素P450含量分别提高为2.48倍和4.25倍,细胞色素b5含量分别提高为1.79倍和3.53倍,均高于敏感品系酶活水平。其结果表明,棕榈蓟马体内酶活的变化是其对杀虫剂产生抗药性的重要因素。
Comparative study on activity of CarE, GST, AchE and content difference of cytochrome P450 and cytochrome b5 in the three enzymes in both susceptible and resistant population of Thrips palmi were conducted through leaf tube residue method to discover resistance multiple of T. palmi against spinetoram and imidacloprid. The results showed that T.palmi had medium level of resistance to imidacloprid and low level to spinetoram, with the value of 12.73 and 9.53 respectively. As for enzyme activities of T. palmi population with resistance to imidacloprid(BK) and spinetoram(DK)compared with susceptible populations, only enzyme activity of AchE of BK decreased to 0.58 times, while that of DK increased to 1.31 times; enzyme activity of CarE of DK and BK increased to 1.46 and 1.79 times, and that of GST increased to 1.40 and 1.59 times, repectively. Contents of cytochrome P450 of DK and BK increased to 2.48 and 4.25 times, and that of cytochrome b5 increased to 1.79 and 3.53 times, repectively. It indicated that changes of enzyme activity in T. palmi were the critical factor of increasing insecticide resistance.
Comparative study on activity of CarE, GST, AchE and content difference of cytochrome P450 and cytochrome b5 in the three enzymes in both susceptible and resistant population of Thrips palmi were conducted through leaf tube residue method to discover resistance multiple of T. palmi against spinetoram and imidacloprid. The results showed that T.palmi had medium level of resistance to imidacloprid and low level to spinetoram, with the value of 12.73 and 9.53 respectively. As for enzyme activities of T. palmi population with resistance to imidacloprid(BK) and spinetoram(DK)compared with susceptible populations, only enzyme activity of AchE of BK decreased to 0.58 times, while that of DK increased to 1.31 times; enzyme activity of CarE of DK and BK increased to 1.46 and 1.79 times, and that of GST increased to 1.40 and 1.59 times, repectively. Contents of cytochrome P450 of DK and BK increased to 2.48 and 4.25 times, and that of cytochrome b5 increased to 1.79 and 3.53 times, repectively. It indicated that changes of enzyme activity in T. palmi were the critical factor of increasing insecticide resistance.
引文
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[20]王圣印,刘永杰,周仙红,等.西花蓟马对吡虫啉抗性机制的研究[J].应用昆虫学报,2011,48(3):559-565.
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[23]Yu S J,Nguyen S N.Detection and biochemical characterization of insecticide resistance in the diamondback moth[J].Pesticide Biochemistry&Physiology,1992,44(1):74-81.
[2]吴佳教,张维球,梁广文.节瓜蓟马生物学特性研究[J].植物保护学报,1996,23(1):13-16.
[3]梁关生,程东美.8种杀虫剂对温室茄瓜蓟马的防治效果研究[J].安徽农业科学,2011,39(8):4585-4586.
[4]张安盛,庄乾营,李丽莉,等.48%乐斯本EC防治日光温室棕榈蓟马研究[J].山东农业科学2013,48(5):115-117.
[5]张安盛,庄乾营,周仙红,等.40%辛硫磷EC防治日光温室棕榈蓟马研究[J].农业灾害研究,2013,3(2-3):18-19.
[6]侯海霞,于毅,周仙红,等.山东省棕榈蓟马田间种群对7种杀虫剂的抗药性[J].植物保护,2015(5):151-154.
[7]张乃芹,于凌春,王明友.十字花科蔬菜棕榈蓟马发生动态及综合防治[J].北方园艺,2013(1):144-146.
[8]龚佑辉.多杀菌素对西花蓟马的亚致死效应研究[D].北京:中国农业科学院,2009.
[9]Rueda A,Shelton A M.Development of a bioassay system for monitoring suscepibility in Thrips tabaci[J].Pest Management Science,2003,59(5):553-558.
[10]王泽华,侯文杰,郝晨彦,等.北京地区西花蓟马田间种群的抗药性监测[J].应用昆虫学报,2011,48(3):542-547.
[11]唐振华,毕强.杀虫剂作用的分子行为[M].上海:上海远东出版社,2003:58-110.
[12]吴刚,尤民生,赵士熙.抗性和敏感小菜蛾谷胱甘肽-S-转移酶和谷胱甘肽的比较[J].福建农林大学学报(自然科学版),2000,29(4):478-481.
[13]邢剑飞,刘艳,颜冬云.昆虫对拟除虫菊酯农药的抗性研究进展[J].环境科学与技术,2010,33(10):68-74.
[14]尹飞,陈焕瑜,冯夏,等.乙基多杀菌素抗性小菜蛾代谢解毒酶酶活性研究[J].应用昆虫学报,2016,53(2):314-319.
[15]曾春祥,王进军.吡虫啉亚致死剂量处理对桃蚜乙酰胆碱酯酶的时间与剂量效应[J].植物保护,2007,33(2):50-54.
[16]潘文亮,党志红,高占林.棉蚜抗吡虫啉品系和敏感品系主要解毒酶活性比较[J].昆虫学报,2003,46(6):793-796.
[17]王光峰,张友军,柏连阳,等.多杀菌素对甜菜夜蛾多酚氧化酶和羧酸酯酶的影响[J].农药学学报,2003,5(2):40-46.
[18]尹显慧,吴青君,李学锋,等.多杀菌素亚致死浓度对小菜蛾解毒酶系活力的影响[J].农药学学报,2008,10(1):28-34.
[19]袁家珪,孙耘芹,冯国蕾,等.家蝇中乙酰胆碱酯酶、羧酸酯酶和多功能氧化酶活性与抗药性的关系[J].昆虫学报,1987,30(2):126-132.
[20]王圣印,刘永杰,周仙红,等.西花蓟马对吡虫啉抗性机制的研究[J].应用昆虫学报,2011,48(3):559-565.
[21]Scott J G,Georghiou G P.Mechanisms responsible for high levels of permethrin resistance in the house fly[J].Pest Management Science,2010,17(3):195-206.
[22]Wen Y,Liu Z,Bao H,et al.Imidacloprid resistance and its mechanisms in field populations of brown planthopper,Nilaparvata lugens,St覽l in China[J].Pesticide Biochemistry&Physiology,2009,94(1):36-42.
[23]Yu S J,Nguyen S N.Detection and biochemical characterization of insecticide resistance in the diamondback moth[J].Pesticide Biochemistry&Physiology,1992,44(1):74-81.