HPLC法测定乙肝扶正清毒颗粒中芍药苷的含量
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摘要
建立了采用高效液相色谱法测定乙肝扶正清毒颗粒中芍药苷含量的方法。采用Agilent ZORBAX SB-C_(18)色谱柱(4.6 mm×250 mm,5μm),柱温25℃,以甲醇-0.05 mol/L磷酸二氢钾(体积比38:62)为流动相,进样量10μL,流速1.0 mL/min,测定波长230 nm。结果显示,芍药苷溶液在0.079 1～0.988 0μg之间呈良好的线性关系(r=0.999 9),加样回收率为98.1%～100.6%,相对标准偏差为1.02%(n=6)。本方法专属性强、灵敏度高、重现性好、操作简便,适用于乙肝扶正清毒颗粒的质量控制。
A method for the determination of paeoniflorin content in Yiganfuzhengqingdu Granules by HPLC was established. HPLC analysis was carried out with Agilent ZORBAX SB-C_(18) chromatographic column(4.6 mm×250 mm,5 μm) and methanol-0.05 mol/L potassium dihydrogen phosphate(38:62, V/V) as mobile phase, the flow rate was 1.0 mL/min, and the column temperature was 25 ℃,injection volume was 10 μL, the detection wavelength was 230 nm. The results showed that the calibration curve was linear in the range of 0.079 1～0.988 0 μg for paeoniflorin(r=0.999 9), the recovery rate was 98.1%～100.6% with RSD 1.02%(n=6). This method is highly specific, sensitive, reproducible and simple, which can be used for quality control of Yiganfuzhengqingdu Granules.
A method for the determination of paeoniflorin content in Yiganfuzhengqingdu Granules by HPLC was established. HPLC analysis was carried out with Agilent ZORBAX SB-C_(18) chromatographic column(4.6 mm×250 mm,5 μm) and methanol-0.05 mol/L potassium dihydrogen phosphate(38:62, V/V) as mobile phase, the flow rate was 1.0 mL/min, and the column temperature was 25 ℃,injection volume was 10 μL, the detection wavelength was 230 nm. The results showed that the calibration curve was linear in the range of 0.079 1～0.988 0 μg for paeoniflorin(r=0.999 9), the recovery rate was 98.1%～100.6% with RSD 1.02%(n=6). This method is highly specific, sensitive, reproducible and simple, which can be used for quality control of Yiganfuzhengqingdu Granules.
引文
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[3]李思雨,刘纪青,简晖.护肾痛风泰颗粒的质量控制方法研究[J].时珍国医国药,2018,29(1):67-68. DOI:10.3969/j.issn.1008-0805.2018.01.022.
[4]刘阳.HPLC法测定复方抗敏胶囊中芍药苷的含量[J].湖南中医杂志,2017,33(12):140-141. DOI:10.16808/j.cnki.issn1003-7705.2017.12.065.
[5]张艳,申丽莎,杨帆,等.赤芍药材中芍药苷含量测定方法的改进[J].中国药业,2017,26(24):28-30. DOI: 10.3969/j.issn.1006-4931.2017.24.009.
[6]张黎娟,李飞.HPLC法测定麻仁软胶囊中芍药苷的含量[J].科学技术创新,2017(31):12-13.
[7]刘青,刘波,丁晓彦.十全大补膏中芍药苷的含量测定[J].山东科学,2017,30(5):28-31. DOI:10.3976/j.issn.1002-4026.2017.05.005.
[8]金林,赵万顺,郭巧生,等.响应面法优化白芍提取工艺的研究[J].中国中药杂志,2015,40(15):2988-2993. DOI:10.4268/cjcmm20151514.
[9]陈永斌,谭梅英,詹利之.星点设计-效应面法优化赤芍中芍药苷提取工艺[J].海峡药学,2015,27(6):22-24. DOI:10.3969/j.issn.1006-3765.2015.06.007.
[10]赵军,樊萍,杨梅音,等.高效液相法测定芍丹乙肝颗粒中芍药苷含量[J].新疆医科大学学报,2004,27(3):293-294. DOI:10.3969/j.issn.1009-5551.2004.03.039.