当归原生粉乙醇灭菌工艺优化及其品质比较研究
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摘要
目的研究确定当归原生粉乙醇灭菌的最优工艺条件并对其品质进行研究。方法以灭菌率为考察指标,乙醇用量、乙醇体积分数、灭菌温度、灭菌时间为因素,在单因素试验的基础上,采用响应面法对灭菌工艺条件进行优化;以菌含量、挥发油提取率及阿魏酸含量为指标,评价优化的乙醇灭菌工艺,以及湿热灭菌法及干热灭菌法对当归原生粉灭菌前后品质的影响。结果最优的乙醇灭菌工艺为乙醇用量34%、乙醇体积分数80%、灭菌温度78℃,灭菌率达99.59%;3种不同灭菌方式的灭菌率为乙醇灭菌法>湿热灭菌法>干热灭菌法;乙醇灭菌的当归原生粉挥发油提取率相比未处理组提高了94.44%,且所含的阿魏酸仅减少8%,而湿热灭菌及干热灭菌处理后挥发油提取率分别下降了55.56%及100%,且阿魏酸完全被破坏。结论响应面法优化的乙醇灭菌工艺能保证当归原生粉微生物含量达要求,并且品质较常规灭菌法优,有望替代当归原生粉的常规灭菌技术。
Objective To optimize the conditions of the ethanol sterilization technology for Angelicae Sinensis Radix(ASR) primary powder and compare of its quality. Methods With the sterilization rate as the index and ethanol usage, ethanol concentration,sterilization temperature and sterilization time as factors, the response surface methodology based on the single-factor experiments was employed to optimize the ethanol sterilization process conditions. With the microbial content, extraction rate of volatile oil and the content of ferulic acid as the indexes, the effect of optimized ethanol sterilization process, the moist heat sterilization method and the dry heat sterilization method on the quality of the sterilization before and after of ASR primary powder were evaluated. Results The optimal parameters of ethanol sterilization technology were as follows: dosage of ethanol of 34%, ethanol concentration of 80% and sterilization temperature of 78 ℃, the sterilization rate was 99.59%. Comparing with the moist heat sterilization method and the dry heat sterilization method, ethanol sterilization method had some advantages, such as higher sterilization rate and better overall yield.Compared with the untreated product, the extraction rate of volatile oil increased 94.44% and the content of ferulic acid decreased 8%after the ethanol sterilization, while decreased 55.56% and 100% of extraction rate of volatile oil after the moist heat sterilization and the dry heat sterilization, respectively; And ferulic acid was destroyed completely. Conclusion The microbial indicators reached the requirement and the quality in chemical composition of ASR primary powder treated with the optimization of the ethanol sterilization process by the response surface method is better than that of conventional sterilization methods. It is expected to be an alternative to conventional sterilization methods of ASR primary powder.
Objective To optimize the conditions of the ethanol sterilization technology for Angelicae Sinensis Radix(ASR) primary powder and compare of its quality. Methods With the sterilization rate as the index and ethanol usage, ethanol concentration,sterilization temperature and sterilization time as factors, the response surface methodology based on the single-factor experiments was employed to optimize the ethanol sterilization process conditions. With the microbial content, extraction rate of volatile oil and the content of ferulic acid as the indexes, the effect of optimized ethanol sterilization process, the moist heat sterilization method and the dry heat sterilization method on the quality of the sterilization before and after of ASR primary powder were evaluated. Results The optimal parameters of ethanol sterilization technology were as follows: dosage of ethanol of 34%, ethanol concentration of 80% and sterilization temperature of 78 ℃, the sterilization rate was 99.59%. Comparing with the moist heat sterilization method and the dry heat sterilization method, ethanol sterilization method had some advantages, such as higher sterilization rate and better overall yield.Compared with the untreated product, the extraction rate of volatile oil increased 94.44% and the content of ferulic acid decreased 8%after the ethanol sterilization, while decreased 55.56% and 100% of extraction rate of volatile oil after the moist heat sterilization and the dry heat sterilization, respectively; And ferulic acid was destroyed completely. Conclusion The microbial indicators reached the requirement and the quality in chemical composition of ASR primary powder treated with the optimization of the ethanol sterilization process by the response surface method is better than that of conventional sterilization methods. It is expected to be an alternative to conventional sterilization methods of ASR primary powder.
引文
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[22]张小飞,詹娟娟,徐传福,等.当归挥发油提取工艺优化及其乳化芳香水成分分析[J].中国实验方剂学杂志,2017,23(5):27-31.
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[25]宋佳,黄飞龙,段树卿,等.Plackett-Burnman设计联合Box-Behnken设计-效应面法优化心神宁片的提取工艺[J].中草药,2016,47(3):430-435.
[26]于淼,柏云娇,代岐昌,等.响应曲面法优化文殊兰中生物碱的提取工艺[J].中草药,2013,44(10):1286-1289.
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[28]栾庆祥,赵杨,周欣,等.单因素试验结合响应面分析法优化杜仲最佳提取工艺[J].药物分析杂志,2013,33(5):859-865.
[29]苏柘僮,刘英,杨明,等.应用Box-Behnken设计优化地榆皂苷的闪式提取工艺研究[J].中草药,2012,43(3):501-504.
[2]杨英来,胡芳,刘小花,等.当归补气活性部位的谱效关系研究[J].中草药,2013,44(23):3346-3351.
[3]周友华,王谷洪,费翼城,等.连续式过热蒸汽灭菌技术在中药粉体生产中的应用[J].机电信息,2018,(8):20-22.
[4]范一灵,李琼琼,房蕊,等.上海地区10种中药饮片微生物污染情况研究[J].中草药,2015,46(13):1908-1913.
[5]王睿陟,李世文.黄芩粉的干热灭菌工艺研究[J].中草药,2013,44(15):2092-2096.
[6]严丹,袁星,吴纯洁,等.中药饮片灭菌的研究现状与思考[J].中草药,2016,47(8):1425-1429.
[7]冯少俊,伍振峰,杨明,等.中药灭菌工艺研究现状及问题分析[J].中草药,2015,46(18):2667-2673.
[8]张萍萍.中药灭菌新方法[J].化学工程与装备,2013(8):186-188.
[9]马方励.常用灭菌技术及其对中药制剂质量的影响[J].中华中医药学刊,2010,28(12):2640-2642.
[10]陈金雅,邓小英,刘春彩,等.压力蒸汽不同灭菌方式、干燥时间与灭菌效果的相关性研究[J].实用临床医药杂志,2013,17(14):154-156.
[11]李跃辉,王银,张水寒,等.灭菌方法对牡丹皮粉体主要成分含量的影响[J].中国医药科学,2012,2(13):41-42.
[12]谭伯森,李惠琴,黄凤婷.不同灭菌法对续断中川续断皂苷VI含量的影响[J].现代医院,2012,12(4):72-73.
[13]王二兵,赵正保,曲婷丽.60Co辐照灭菌对止嗽立效口服液主要成分的影响[J].中成药,2013,35(9):2056-2058.
[14]张立雯,江英桥,林彤,等.中药辐照检测研究现状概述[J].中药材,2011,34(3):482-487.
[15]陈佳,金红宇,田金改,等.60Co-γ射线灭菌后中药材中的辐照残留考察[J].药物分析杂志,2012,32(9):1694-1696.
[16]曾翠梅,林春颖,黄凤婷.辐照灭菌工艺对熟地黄中梓醇含量的影响[J].海峡药学,2013,25(10):59-60.
[17]王丽萍,杨金华,陈天朝.乙醇蒸气灭菌中药饮片的方法探讨[J].中国药房,2012,23(35):3308-3309.
[18]吕鹏,白明学,陈天朝.中药固体制剂乙醇蒸汽灭菌探讨[J].中国中医药现代远程教育,2014,12(17):152-154.
[19]杨金华,施钧瀚.乙醇气体灭菌法在我院中药制剂中的应用研究[J].中医研究,2011,24(1):28-31.
[20]王学成,伍振峰,杨明,等.臭氧与硫熏处理后当归品质变化情况的比较[J].中国实验方剂学杂志,2018,24(3):36-40.
[21]李江,杨星钢,荆恒攀,等.星点设计-效应面法优化银杏叶总黄酮双层渗透泵控释片处方[J].中草药,2014,45(12):1702-1708.
[22]张小飞,詹娟娟,徐传福,等.当归挥发油提取工艺优化及其乳化芳香水成分分析[J].中国实验方剂学杂志,2017,23(5):27-31.
[23]李锡岩.酒精浓度高不等于消毒作用强[J].家庭医学,2005(3):25.
[24]宋艺君,郭涛,朱亚亚,等.响应面法优化醋延胡索微波炮制工艺研究[J].中草药,2017,48(20):4261-4267.
[25]宋佳,黄飞龙,段树卿,等.Plackett-Burnman设计联合Box-Behnken设计-效应面法优化心神宁片的提取工艺[J].中草药,2016,47(3):430-435.
[26]于淼,柏云娇,代岐昌,等.响应曲面法优化文殊兰中生物碱的提取工艺[J].中草药,2013,44(10):1286-1289.
[27]陈敬,温庆果,刘韶,等.正交设计与响应面法优化壳聚糖对莲子心提取液除杂工艺对比研究[J].中草药,2012,43(11):2183-2188.
[28]栾庆祥,赵杨,周欣,等.单因素试验结合响应面分析法优化杜仲最佳提取工艺[J].药物分析杂志,2013,33(5):859-865.
[29]苏柘僮,刘英,杨明,等.应用Box-Behnken设计优化地榆皂苷的闪式提取工艺研究[J].中草药,2012,43(3):501-504.