飞扬肠胃炎胶囊体外抑菌作用研究
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摘要
目的探讨飞扬肠胃炎胶囊的体外抑菌效果。方法采用试管二倍稀释法联合琼脂平板法,以头孢曲松钠为阳性对照药,测定飞扬肠胃炎胶囊对大肠埃希菌、金黄色葡萄球菌、粪肠球菌、铜绿假单胞菌、β-溶血性链球菌、白色念珠菌的最低抑菌浓度(MIC)和最低杀菌浓度(MBC)。结果飞扬肠胃炎胶囊对大肠埃希菌(MIC为6.25 mg/mL)、金黄色葡萄球菌(MIC为1.56 mg/mL)、粪肠球菌(MIC为25.00 mg/mL)、铜绿假单胞菌(MIC为6.25 mg/mL)、β-溶血性链球菌(MIC为12.50 mg/mL)的体外抑菌作用较强,对白色念珠菌(MIC为100.00 mg/mL)的体外抑菌作用较弱。结论飞扬肠胃炎胶囊对大肠埃希菌、金黄色葡萄球菌、粪肠球菌、铜绿假单胞菌、β-溶血性链球菌具有较强的体外抑菌作用。
Objective To investigate the antimicrobial activity in vitro of Feiyang Changweiyan Capsules. Methods The tube double dilution method and agar plate method were used to determine the minimum inhibitory concentrations(MIC)and minimum bactericidal concentrations(MBC) of Feiyang Changweiyan Capsules against Escherichia coil, Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa, β-hemolytic streptococcus and Candida albicans, taking Ceftri axone as positive control drug. Results The antibacterial activity of Feiyang Changweiyan Capsules against Escherichia coil(MIC was 6.25 mg/mL), Staphylococcus aureus(MIC was 1.56 mg/mL), Enterococcus faecalis(MIC was25.00 mg/mL), Pseudomonas aeruginosa(MIC was 6.25 mg/mL) and β-hemolytic streptococcus(MIC was 12.50 mg/mL)was stronger, which of Candida albicans(MIC was 100.00 mg/mL) was weaker. Conclusion Feiyang Changweiyan Capsules have stronger antimicrobial activity in vitro for Escherichia coil, Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa and β-hemolytic streptococcus.
Objective To investigate the antimicrobial activity in vitro of Feiyang Changweiyan Capsules. Methods The tube double dilution method and agar plate method were used to determine the minimum inhibitory concentrations(MIC)and minimum bactericidal concentrations(MBC) of Feiyang Changweiyan Capsules against Escherichia coil, Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa, β-hemolytic streptococcus and Candida albicans, taking Ceftri axone as positive control drug. Results The antibacterial activity of Feiyang Changweiyan Capsules against Escherichia coil(MIC was 6.25 mg/mL), Staphylococcus aureus(MIC was 1.56 mg/mL), Enterococcus faecalis(MIC was25.00 mg/mL), Pseudomonas aeruginosa(MIC was 6.25 mg/mL) and β-hemolytic streptococcus(MIC was 12.50 mg/mL)was stronger, which of Candida albicans(MIC was 100.00 mg/mL) was weaker. Conclusion Feiyang Changweiyan Capsules have stronger antimicrobial activity in vitro for Escherichia coil, Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa and β-hemolytic streptococcus.
引文
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[16]王圆圆,刘雅楠,唐天瀛,等.胃肠道菌群与慢性萎缩性胃炎的关系概述[J].世界华人消化杂志,2016,24(10):1526-1531.
[17]江森.女性生殖系统炎症病原体的变迁[J].中国实用妇科与产科杂志,1998,14(6):341-342.
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[19]熊优,王雅琪,胡彦君,等.论“药辅合一”的传统与现代应用研究[J].世界科学技术:中医药现代化,2016,18(10):1765-1769.
[20]Andrews JM.Determination of minimum inhibitory concentrations[J].J Antimicrob Chemother,2001,48 Suppl 1:5-16.
[2]沈洁,宋捷民.飞扬草的本草考证及其研究进展[J].中国民族民间医药,2010,2(23):45-46.
[3]Sandeep BP,Nilofar SN,Chandrakant SM.Review on phytochemistry and pharmacologyical aspect of euphorbia hirta linn[J].A J Pharm Res Health Care,2009,1(1):113-133.
[4]Kumar S,Malhotra R,Kumar D.Euphorbia hirta:its chemistry,traditional and medicinal uses,and pharmacological activities[J].Pharmacogn Rev,2010,4(7):58-61.
[5]Titilope KK,Rashidat EA,Christiana OC.In-vitro antimicrobial activities of Euphorbia hirta against some clinical isolates[J].Agr Biol J N Am,2012,3(4):169-174.
[6]de Boer HJ,Kool A,Broberg A,et al.Anti-fungal and antibacterial activity of some herbal remedies from Tanzania[J].J Ethnopharmacol,2005,96(3):461-469.
[7]国家中医药管理局.中华本草(第二卷)[M].上海:上海科学技术出版社,1999:647-649.
[8]任国珍,何世东.火炭母复方为主治疗小儿急性细菌性痢疾52例[J].广西中医药,2001,24(1):32.
[9]欧阳蒲月,朱翠霞,陈功锡,等.火炭母提取物抑菌活性的初步研究[J].化学与生物工程,2012,29(4):37-40.
[10]戴卫波,梅全喜,曾聪彦.救必应化学成分研究及临床应用进展[J].亚太传统医药,2008,4(12):137-139.
[11]张榕文,黄兆胜,范庆亚,等.救必应抑菌抗炎有效部位筛选[J].中华中医药学刊,2008,26(8):1820-1822.
[12]郭丽双,葛立男,宋宝辉,等.中药体外抑菌实验检测方法的比较[J].中医学报,2011,26(10):1207-1208.
[13]Cockerill FR.Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically:approved standard[M].Clinical and Laboratory Standards Institute,2002.
[14]胡泽涛,陈思韵,阮晓枫.脾肾相关理论下肠道及阴道微生态平衡的关系探讨[J].时珍国医国药,2016,27(2):419-421.
[15]凌宗欣,刘霞,项春生.女性生殖道微生态屏障[J].中国微生态学杂志,2011,23(12):1136-1139.
[16]王圆圆,刘雅楠,唐天瀛,等.胃肠道菌群与慢性萎缩性胃炎的关系概述[J].世界华人消化杂志,2016,24(10):1526-1531.
[17]江森.女性生殖系统炎症病原体的变迁[J].中国实用妇科与产科杂志,1998,14(6):341-342.
[18]刘朝辉,董悦.需氧菌阴道炎菌群及治疗效果的临床研究[J].现代妇产科进展,2009,18(11):832-834.
[19]熊优,王雅琪,胡彦君,等.论“药辅合一”的传统与现代应用研究[J].世界科学技术:中医药现代化,2016,18(10):1765-1769.
[20]Andrews JM.Determination of minimum inhibitory concentrations[J].J Antimicrob Chemother,2001,48 Suppl 1:5-16.