飞扬肠胃炎胶囊对盆腔炎性后遗症大鼠的治疗作用及机制探讨
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摘要
目的:观察飞扬肠胃炎胶囊对盆腔炎性后遗症大鼠的药效及其作用机制。方法:15%苯酚胶浆注入大鼠右侧子宫复制盆腔炎性后遗症动物模型,造模15d后,将动物随机分为假手术组,妇科千金胶囊组(1.2g/kg),飞扬肠胃炎胶囊低剂量组(0.6g/kg)、飞扬肠胃炎胶囊中剂量组(1.2g/kg)、飞扬肠胃炎胶囊高剂量组(2.4g/kg)和模型组,灌胃给药30d后,观察大鼠子宫外观形态变化,HE染色评价病理改变情况,ELISA检测血清中白介素1-β(IL-1β)、肿瘤坏死因子-α(TNF-α)含量,免疫组化检测子宫中核转录因子(NF-ΚB)、天冬氨酸特异性半胱氨酸蛋白酶-3(Caspase-3)的表达。结果:与假手术组相比,模型组大鼠血清中IL-1β、TNF-α显著升高,组织中NF-ΚB、Caspase-3表达升高,经飞扬肠胃炎胶囊中剂量(1.2g/kg)、飞扬肠胃炎胶囊高剂量(2.4g/kg)治疗后,血清中IL-1β、TNF-α含量显著降低,组织中NF-ΚB表达降低,Caspase-3表达升高。结论:飞扬肠胃炎胶囊中(1.2g/kg)、飞扬肠胃炎胶囊高剂量(2.4g/kg)对盆腔炎性后遗症大鼠有良好的治疗作用,其作用机制可能与其抑制NF-ΚB通路激活和上调凋亡通路上的Caspase-3蛋白表达有关。
Objective: To explore the mechanism of Feiyang Changweiyan Capsules on the rats with sequelae of pelvic inflammatory disease by observing inflammatory cytokine and proteins. Methods: Rats with sequelae of pelvic inflammatory disease were caused by injection of 15% Phenol mucilage into the right uterus of rats. After 15 days,rats were randomly divided into several groups as follows: the control group,Qianjin Capsules(1. 2 g/kg),low dosage of Feiyang Changweiyan Capsules(0. 6 g/kg),middle dosage of Feiyang Changweiyan Capsules(1. 2 g/kg),high dosage of Feiyang Changweiyan Capsules(2. 4 g/kg) and the model group,rats in each group were given corresponding drug or pure water once a day for 30 days by intragastric administration(i. g.),pathological changes of endometrium were observed and concentrations of IL-1βand TNF-α were examined in serum by ELISA assay kits. Expressions of NF-ΚB and Caspase-3 in sequelae of pelvic inflammatory disease rats were measured by immunohistochemistry. Results: Compared with the control group,levels of IL-1βand TNF-α in serum of model rats were elevated,and expressions of NF-ΚB and Caspase-3 were up-regulated. After Feiyang Changweiyan Capsules treatment,serum IL-1βand TNF-α were dramatically decreased compared with the model group,and the immune expression of NF-κB was significantly reduced and Caspase-3 expression was obviously promoted. Conclusion: The dosages of 1. 2 g/kg and 2. 4 g/kg of Feiyang Changweiyan Capsules can alleviate the sequelae of pelvic inflammatory disease by down-regulation the expression of NF-κB and a series of inflammatory cytokines,at the same time,up-regulation the expression of Caspase-3.
Objective: To explore the mechanism of Feiyang Changweiyan Capsules on the rats with sequelae of pelvic inflammatory disease by observing inflammatory cytokine and proteins. Methods: Rats with sequelae of pelvic inflammatory disease were caused by injection of 15% Phenol mucilage into the right uterus of rats. After 15 days,rats were randomly divided into several groups as follows: the control group,Qianjin Capsules(1. 2 g/kg),low dosage of Feiyang Changweiyan Capsules(0. 6 g/kg),middle dosage of Feiyang Changweiyan Capsules(1. 2 g/kg),high dosage of Feiyang Changweiyan Capsules(2. 4 g/kg) and the model group,rats in each group were given corresponding drug or pure water once a day for 30 days by intragastric administration(i. g.),pathological changes of endometrium were observed and concentrations of IL-1βand TNF-α were examined in serum by ELISA assay kits. Expressions of NF-ΚB and Caspase-3 in sequelae of pelvic inflammatory disease rats were measured by immunohistochemistry. Results: Compared with the control group,levels of IL-1βand TNF-α in serum of model rats were elevated,and expressions of NF-ΚB and Caspase-3 were up-regulated. After Feiyang Changweiyan Capsules treatment,serum IL-1βand TNF-α were dramatically decreased compared with the model group,and the immune expression of NF-κB was significantly reduced and Caspase-3 expression was obviously promoted. Conclusion: The dosages of 1. 2 g/kg and 2. 4 g/kg of Feiyang Changweiyan Capsules can alleviate the sequelae of pelvic inflammatory disease by down-regulation the expression of NF-κB and a series of inflammatory cytokines,at the same time,up-regulation the expression of Caspase-3.
引文
1阮越容,范氏凤,董莉.中医治疗慢性盆腔炎临床研究进展.山东中医药大学学报,2016;40(3)∶293~296
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10 王圆圆,刘雅楠,唐天瀛,等.胃肠道菌群与慢性萎缩性胃炎的关系概述.世界华人消化杂志,2016;24(10)∶1526~1531
11 孙兰,林楠,吕耀中,等.桂枝茯苓胶囊对大鼠慢性盆腔炎的影响.中药药理与临床,2013;29(4)∶21~24
12 杨英英,胡晓华.胡晓华教授治疗慢性盆腔炎的经验介绍.中国中医药现代远程教育,2015;13(4)∶39~40
13 刘文娥,张婉妮,陈燕霞.关于盆腔炎性疾病动物模型的研究概况.医学综述,2014;20(23)∶4295~4297
14 Lv X,Fu K,Li W,et al.TIIA attenuates LPS induced mouse endometritis by suppressing the NF-κB signaling pathway.Can J Physiol Pharmacol,2015;93(11)∶967~971
15 Pan dith J avid Iqbal.Phytochemical screening of certain plant species of Agra City.J Drug Deliv and Ther,2012;2(4)∶135~138
16 蔡家驹,曾聪彦,梅全喜.火炭母水提物抗炎、镇痛作用的实验研究.时珍国医国药,2017;28(1)∶100~102
17 杨利林,罗颂平.罗颂平教授应用岭南草药经验总结.新中医,2014;46 (10)∶28~30
18 彭喻嫄,梁敬钰,冯锋.救必应的研究进展.海峡药学,2014;26(12)∶7~8
2 李波.飞扬肠胃炎片联合左氧氟沙星治疗急慢性胃肠炎的疗效观察.中国卫生标准管理,2015;6(24)∶130~131
3 Kumar S,Malhotra R,Kumar D.Euphorbia hirta:Its chemistry,traditional and medicinal uses,and pharmacological activities.Pharmacognosy Reviews,2010;4(7)∶58~60
4 范文昌,梅全喜,欧秀华,等.12种广东地产清热解毒药材的抗炎作用研究.中国药业,2011;20(8)∶28~29
5 欧阳蒲月,朱翠霞,陈功锡,等.火炭母提取物抑菌活性的初步研究.化学与生物工程,2012;29(4)∶37~44
6 戴卫波,梅全喜,曾聪彦.救必应化学成分研究及临床应用进展.亚太传统医药,2008;4(12)∶137~139
7 张榕文,黄兆胜,范庆亚,等.救必应抑菌抗炎有效部位筛选.中华中医药学刊,2008;(8)∶1820~1822
8 胡泽涛,陈思韵,阮晓枫.脾肾相关理论下肠道及阴道微生态平衡的关系探讨.时珍国医国药,2016;27(2)∶419~421
9 凌宗欣,刘霞,项春生.女性生殖道微生态屏障.中国微生态学杂志,2011;23(12)∶1136~1139
10 王圆圆,刘雅楠,唐天瀛,等.胃肠道菌群与慢性萎缩性胃炎的关系概述.世界华人消化杂志,2016;24(10)∶1526~1531
11 孙兰,林楠,吕耀中,等.桂枝茯苓胶囊对大鼠慢性盆腔炎的影响.中药药理与临床,2013;29(4)∶21~24
12 杨英英,胡晓华.胡晓华教授治疗慢性盆腔炎的经验介绍.中国中医药现代远程教育,2015;13(4)∶39~40
13 刘文娥,张婉妮,陈燕霞.关于盆腔炎性疾病动物模型的研究概况.医学综述,2014;20(23)∶4295~4297
14 Lv X,Fu K,Li W,et al.TIIA attenuates LPS induced mouse endometritis by suppressing the NF-κB signaling pathway.Can J Physiol Pharmacol,2015;93(11)∶967~971
15 Pan dith J avid Iqbal.Phytochemical screening of certain plant species of Agra City.J Drug Deliv and Ther,2012;2(4)∶135~138
16 蔡家驹,曾聪彦,梅全喜.火炭母水提物抗炎、镇痛作用的实验研究.时珍国医国药,2017;28(1)∶100~102
17 杨利林,罗颂平.罗颂平教授应用岭南草药经验总结.新中医,2014;46 (10)∶28~30
18 彭喻嫄,梁敬钰,冯锋.救必应的研究进展.海峡药学,2014;26(12)∶7~8