摘要
目的:观察飞扬肠胃炎胶囊对盆腔炎性后遗症大鼠的药效及其作用机制。方法:15%苯酚胶浆注入大鼠右侧子宫复制盆腔炎性后遗症动物模型,造模15d后,将动物随机分为假手术组,妇科千金胶囊组(1.2g/kg),飞扬肠胃炎胶囊低剂量组(0.6g/kg)、飞扬肠胃炎胶囊中剂量组(1.2g/kg)、飞扬肠胃炎胶囊高剂量组(2.4g/kg)和模型组,灌胃给药30d后,观察大鼠子宫外观形态变化,HE染色评价病理改变情况,ELISA检测血清中白介素1-β(IL-1β)、肿瘤坏死因子-α(TNF-α)含量,免疫组化检测子宫中核转录因子(NF-ΚB)、天冬氨酸特异性半胱氨酸蛋白酶-3(Caspase-3)的表达。结果:与假手术组相比,模型组大鼠血清中IL-1β、TNF-α显著升高,组织中NF-ΚB、Caspase-3表达升高,经飞扬肠胃炎胶囊中剂量(1.2g/kg)、飞扬肠胃炎胶囊高剂量(2.4g/kg)治疗后,血清中IL-1β、TNF-α含量显著降低,组织中NF-ΚB表达降低,Caspase-3表达升高。结论:飞扬肠胃炎胶囊中(1.2g/kg)、飞扬肠胃炎胶囊高剂量(2.4g/kg)对盆腔炎性后遗症大鼠有良好的治疗作用,其作用机制可能与其抑制NF-ΚB通路激活和上调凋亡通路上的Caspase-3蛋白表达有关。
Objective: To explore the mechanism of Feiyang Changweiyan Capsules on the rats with sequelae of pelvic inflammatory disease by observing inflammatory cytokine and proteins. Methods: Rats with sequelae of pelvic inflammatory disease were caused by injection of 15% Phenol mucilage into the right uterus of rats. After 15 days,rats were randomly divided into several groups as follows: the control group,Qianjin Capsules(1. 2 g/kg),low dosage of Feiyang Changweiyan Capsules(0. 6 g/kg),middle dosage of Feiyang Changweiyan Capsules(1. 2 g/kg),high dosage of Feiyang Changweiyan Capsules(2. 4 g/kg) and the model group,rats in each group were given corresponding drug or pure water once a day for 30 days by intragastric administration(i. g.),pathological changes of endometrium were observed and concentrations of IL-1βand TNF-α were examined in serum by ELISA assay kits. Expressions of NF-ΚB and Caspase-3 in sequelae of pelvic inflammatory disease rats were measured by immunohistochemistry. Results: Compared with the control group,levels of IL-1βand TNF-α in serum of model rats were elevated,and expressions of NF-ΚB and Caspase-3 were up-regulated. After Feiyang Changweiyan Capsules treatment,serum IL-1βand TNF-α were dramatically decreased compared with the model group,and the immune expression of NF-κB was significantly reduced and Caspase-3 expression was obviously promoted. Conclusion: The dosages of 1. 2 g/kg and 2. 4 g/kg of Feiyang Changweiyan Capsules can alleviate the sequelae of pelvic inflammatory disease by down-regulation the expression of NF-κB and a series of inflammatory cytokines,at the same time,up-regulation the expression of Caspase-3.
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