飞扬肠胃炎胶囊及其主要成分的体外抗菌作用
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摘要
目的探究飞扬肠胃炎胶囊及其主要成分对常见细菌的体外抗菌作用,探寻飞扬肠胃炎胶囊的敏感菌株及主要抗菌成分。方法采用倍比稀释测定飞扬肠胃炎胶囊、缺药味药物、没食子酸、鞣花酸、紫丁香苷的最小抑菌浓度(MIC)。结果飞扬肠胃炎胶囊对金黄色葡萄球菌的抑制作用最显著,MIC值为1.6 mg/mL;对福氏志贺菌和肠炎沙门菌的抑制作用显著,MIC值为3.1 mg/mL;对大肠埃希菌、铜绿假单胞菌、大肠埃希菌(产肠毒素株)、伤寒沙门、鼠伤寒沙门的抑制作用较显著,MIC值为6.3 mg/mL;对肺炎克雷伯菌的抑制作用极弱,MIC值为50.0 mg/mL;对白色念珠菌几乎无抑制作用,MIC值>100.0 mg/mL;飞扬肠胃炎胶囊对临床菌包括大肠埃希菌、伤寒沙门菌、肠炎沙门菌、福氏志贺菌、变形杆菌均表现出较强的抑制作用,MIC值分别为6.3、6.3、3.1、3.1、12.5 mg/mL。结论飞扬肠胃炎胶囊及主要成分没食子酸、鞣花酸、紫丁香苷均具有较强的抑菌作用,敏感菌株为金黄色葡萄球菌、福氏志贺菌、肠炎沙门菌、大肠埃希菌、铜绿假单胞菌、大肠埃希菌(产肠毒素株)、伤寒沙门、鼠伤寒沙门和普通变形杆菌;飞扬草、火炭母和救必应均有一定的抗菌作用,其中救必应的抗菌作用略优于其他二味中药,且救必应中发挥抗菌作用的主要成分可能为紫丁香苷。
Objective To explore the in vitro antimicrobial activity of Feiyang Changweiyan Capsules and its main components against common bacteria, and to explore the sensitive strains and main antimicrobial components of Feiyang Changweiyan Capsules. Methods Minimum inhibitory concentration(MIC) of Feiyang Changweiyan Capsules, drug-deficient drugs, gallic acid, ellagic acid and syringin were determined by multiple dilution method. Results Feiyang Changweiyan Capsules had the most significant inhibitory effect on Staphylococcus aureus with MIC value of 1.6 mg/mL,it had significant inhibitory effect on Shigella flexneri and Salmonella enteritidis with MIC value of 3.1 mg/mL, and it had significant inhibitory effect on Escherichia coli, Pseudomonas aeruginosa, Escherichia coli(enterotoxin producing strain),Salmonella typhimurium and Salmonella typhimurium with MIC value of 6.3 mg/mL. The MIC value was 50.0 mg/mL for Klebsiella pneumoniae, almost no inhibition for Candida albicans, MIC value was more than 100.0 mg/mL; Feiyang Changweiyan Capsules showed strong inhibition for clinical bacteria including Escherichia coli, Salmonella typhi,Salmonella enteritidis, Shigella flexneri and Proteus vulgaris, MIC values were 6.3, 6.3, 3.1, 3.1 and 12.5 mg/mL, respectively. Conclusion Feiyang Changweiyan Capsules and its main components, gallic acid, ellagic acid and syringin,have strong antibacterial activity. The sensitive strains are Staphylococcus aureus, Shigella flexneri, Salmonella enteritidis, Escherichia coli, Pseudomonas aeruginosa, Escherichia coli(enterotoxin-producing strains), Salmonella typhimurium, Salmonella typhimurium and Proteus vulgaris. Euphorbia hirta L., Polygonum chinense L. and Ilex rotunda thunb. all have certain antibacterial activity, among which the antimicrobial effect of Ilex rotunda thunb. is slightly bet-ter than that of other two traditional Chinese medicines,and the main component of the antibacterial activity of Ilex rotunda thunb may be syringin.
Objective To explore the in vitro antimicrobial activity of Feiyang Changweiyan Capsules and its main components against common bacteria, and to explore the sensitive strains and main antimicrobial components of Feiyang Changweiyan Capsules. Methods Minimum inhibitory concentration(MIC) of Feiyang Changweiyan Capsules, drug-deficient drugs, gallic acid, ellagic acid and syringin were determined by multiple dilution method. Results Feiyang Changweiyan Capsules had the most significant inhibitory effect on Staphylococcus aureus with MIC value of 1.6 mg/mL,it had significant inhibitory effect on Shigella flexneri and Salmonella enteritidis with MIC value of 3.1 mg/mL, and it had significant inhibitory effect on Escherichia coli, Pseudomonas aeruginosa, Escherichia coli(enterotoxin producing strain),Salmonella typhimurium and Salmonella typhimurium with MIC value of 6.3 mg/mL. The MIC value was 50.0 mg/mL for Klebsiella pneumoniae, almost no inhibition for Candida albicans, MIC value was more than 100.0 mg/mL; Feiyang Changweiyan Capsules showed strong inhibition for clinical bacteria including Escherichia coli, Salmonella typhi,Salmonella enteritidis, Shigella flexneri and Proteus vulgaris, MIC values were 6.3, 6.3, 3.1, 3.1 and 12.5 mg/mL, respectively. Conclusion Feiyang Changweiyan Capsules and its main components, gallic acid, ellagic acid and syringin,have strong antibacterial activity. The sensitive strains are Staphylococcus aureus, Shigella flexneri, Salmonella enteritidis, Escherichia coli, Pseudomonas aeruginosa, Escherichia coli(enterotoxin-producing strains), Salmonella typhimurium, Salmonella typhimurium and Proteus vulgaris. Euphorbia hirta L., Polygonum chinense L. and Ilex rotunda thunb. all have certain antibacterial activity, among which the antimicrobial effect of Ilex rotunda thunb. is slightly bet-ter than that of other two traditional Chinese medicines,and the main component of the antibacterial activity of Ilex rotunda thunb may be syringin.
引文
[1]李波.飞扬肠胃炎片联合左氧氟沙星治疗急慢性胃肠炎的疗效观察[J].中国卫生标准管理,2015,6(24):130-131.
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[13]Opipari A,Franchi L.Role of Inflammasomes in Intestinal Inflammation and Crohn′s Disease[J].Inflamm Bowel Dis,2015,21(1):173-181.
[14]Xie X,Yang M,Ding Y,et al.Microbial infection,inflammation and epithelial ovarian cancer[J].Oncol Lett,2017,14(2):1911-1919.
[15]Mohebi R,Esmaili K,Sadeghifard N,et al.What about Urinary Tract Infections and its Antibiotic Resistance Bacteria in Ilam,Iran?[J].Infectious Disorders Drug Targets,2018,6(22):1-10.
[16]Jiang S,Zeng J,Zhou X,et al.Drug Resistance and Gene Transfer Mechanisms in Respiratory/Oral Bacteria[J].JDent Res,2018,3(14):1-17.
[17]丛朵.急性胃肠炎的病因治疗及护理体会[J].临床医药文献杂志,2014,1(13):2546.
[18]诸宏伟,孙琦,沈怀云,等.小儿细菌性痢疾临床特点及病原学分析[J].中国微生态学杂志,2013,25(5):574-576.
[19]刘策,涂银萍,白书媛,等.临床诊断急性细菌性痢疾的流行病学、病原学及临床特点[J].医学动物防制,2017,33(1):40-43.
[20]谢飞飞,何雅青,程锦泉,等.2006年深圳市急性肠胃炎的流行病学和病毒学调查[J].热带医学杂志,2013,13(4):397-401.
[21]李瑶,杨倩,周丹,等.飞扬肠胃炎胶囊体外抑菌作用研究[J].中国医药导报,2017,14(28):21-24.
[22]张均田.现代药理实验方法[M].北京:北京医科大学中国协和医科大学联合出版社,1999:1409-1452.
[2]Attah SK,Ayeh-Kumi PF,Sittie AA,et al.Extracts of Euphorbia hirta Linn.(Euphorbiaceae)and Rauvolfia vomitoria Afzel(Apocynaceae)demonstrate activities against Onchocerca volvulus Microfilariae in vitro[J].BMC Complement Altern Med,2013,13(1):66.
[3]Sudhakar M,Rao C,Rao PM,et al.Antimicrobial activity of Caesalpinia pulcherrima,Euphorbia hirta and Asystasia gangeticum[J].Fitoterapia,2006,77(5):378-380.
[4]Tran TT,Kim M,Jang Y,et al.Characterization and mechanisms of anti-influenza virus metabolites isolated from the Vietnamese medicinal plant Polygonum chinense[J].BMC Complement Altern Med,2017,17(1):162.
[5]欧阳蒲月,朱翠霞,陈功锡,等.火炭母提取物抑菌活性的初步研究[J].化学与生物工程,2012,4(8):37-40.
[6]Chen XQ,Zan K,Yang J,et al.Quantitative analysis of triterpenoids in different parts of Ilex hainanensis,Ilex stewardii and Ilex pubescens using HPLC-ELSD and HPLC-MSn and antibacterial activity[J].Food Chem,2011,126(3):1454-1459.
[7]沈洁,宋捷民.飞扬草的本草考证及其研究进展[J].中国民族民间医药,2010,2(23):45-46.
[8]管远志.医学微生物学实验技术[M].北京:化学工业出版社,2005.
[9]胡少辉,阚威,周长卿,等.中草药对奶牛隐性乳房炎的体外抑菌试验[J].甘肃农业大学学报,2014,6(3):17-22.
[10]Woudstra L,Juffermans LJM,Van Rossum AC,et al.Infectious myocarditis:the role of the cardiac vasculature[J].Heart Fail Rev,2018,23(4):583-595.
[11]Kelly C,Chalmers JD,Crossingham I,et al.Macrolide antibiotics for bronchiectasis[J].Cochrane Database Syst Rev,2018,3(2):1465-1858.
[12]Casta觡o-Rodríguez N,Underwood AP,Merif J,et al.Gut Microbiome Analysis Identifies Potential Etiological Factors in Acute Gastroenteritis[J].Infect Immun,2018,86(7):1-18.
[13]Opipari A,Franchi L.Role of Inflammasomes in Intestinal Inflammation and Crohn′s Disease[J].Inflamm Bowel Dis,2015,21(1):173-181.
[14]Xie X,Yang M,Ding Y,et al.Microbial infection,inflammation and epithelial ovarian cancer[J].Oncol Lett,2017,14(2):1911-1919.
[15]Mohebi R,Esmaili K,Sadeghifard N,et al.What about Urinary Tract Infections and its Antibiotic Resistance Bacteria in Ilam,Iran?[J].Infectious Disorders Drug Targets,2018,6(22):1-10.
[16]Jiang S,Zeng J,Zhou X,et al.Drug Resistance and Gene Transfer Mechanisms in Respiratory/Oral Bacteria[J].JDent Res,2018,3(14):1-17.
[17]丛朵.急性胃肠炎的病因治疗及护理体会[J].临床医药文献杂志,2014,1(13):2546.
[18]诸宏伟,孙琦,沈怀云,等.小儿细菌性痢疾临床特点及病原学分析[J].中国微生态学杂志,2013,25(5):574-576.
[19]刘策,涂银萍,白书媛,等.临床诊断急性细菌性痢疾的流行病学、病原学及临床特点[J].医学动物防制,2017,33(1):40-43.
[20]谢飞飞,何雅青,程锦泉,等.2006年深圳市急性肠胃炎的流行病学和病毒学调查[J].热带医学杂志,2013,13(4):397-401.
[21]李瑶,杨倩,周丹,等.飞扬肠胃炎胶囊体外抑菌作用研究[J].中国医药导报,2017,14(28):21-24.
[22]张均田.现代药理实验方法[M].北京:北京医科大学中国协和医科大学联合出版社,1999:1409-1452.