基于主成分分析的前胡类药材HPLC特征图谱研究
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摘要
目的:利用高效液相色谱技术,建立前胡及其易混用品紫花前胡和硬前胡的特征图谱,结合主成分分析,用于该类药材的鉴别和质量控制。方法:采用Aglient TC C18色谱柱(4.5 mm×250 mm,5μm),以水(A)-甲醇(B)为流动相,梯度洗脱(0～15 min,40%B;15～16 min,40%B→48%B;16～26 min,48%B→75%B;26～55 min,75%B→95%B;55～56 min,95%B→99%B;56～60 min,99%B,流速1.0mL·min~(-1),检测波长325 nm,柱温30℃,进样量5μL,分别建立不同产地前胡、紫花前胡、硬前胡的HPLC特征图谱,并通过Chempattern及Ezinfo化学计量学软件对所采集的数据进行主成分分析。结果:主成分分析结果显示前胡及其易混用品分别聚在不同的区域,所含化学成分存在明显差异。结论:本方法稳定性、重现性好,所建立的特征图谱专属性强,可用于前胡及其易混用品的鉴别和质量控制。
Objective:To establish HPLC characteristic fingerprints of Peucedani Radix and its promiscuous herbs(Peucedani Decursivi Radix and Peucedani Subglabri Radix),and to identify and control the quality of the medicinal materials by principle component analysis(PC A).Methods:The HPLC analysis was carried out on an Aglient TC-C18 column(4.6 mm × 250 mm,5 μm)with water(A)-methanol(B)as mobile phase by gradient elution(0-15 min,40%B; 15-16 min,40%B→48%B; 16-26 min,48%B→75%B;26-55 min,75%B→95%B;55-56 min,95%B→99%B;56-60 min,99%B),at a flow rate of 1.0 mL·min~(-1). The column temperature was set at30 ℃,the detection wavelength was set at 325 nm,and the injection volume was 5 μL.HPLC characteristic fingerprints of Peucedani Radix,Peucedani Decursivi Radix and Peucedani Sub glabri Radix from different habitats were established,respectively.The obtained data were analyzed by Chempattern and Ezinfo chemometrics software.Results:PC A were used to analyze the LC-MS data,the results showed that Peucedani Radix,Peucedani Decursivi Radix and Peucedani Subglabri Radix clustered in different zones respectively,and chemical compositions in Peucedani Radix and its promiscuous herbs varied obviously.Conclusion:The proposed method is stable and reproducible,and the established characteristic fingerprint is specific,which can be used for identification and quality control of Peucedani Radix and its promiscuous herbs.
Objective:To establish HPLC characteristic fingerprints of Peucedani Radix and its promiscuous herbs(Peucedani Decursivi Radix and Peucedani Subglabri Radix),and to identify and control the quality of the medicinal materials by principle component analysis(PC A).Methods:The HPLC analysis was carried out on an Aglient TC-C18 column(4.6 mm × 250 mm,5 μm)with water(A)-methanol(B)as mobile phase by gradient elution(0-15 min,40%B; 15-16 min,40%B→48%B; 16-26 min,48%B→75%B;26-55 min,75%B→95%B;55-56 min,95%B→99%B;56-60 min,99%B),at a flow rate of 1.0 mL·min~(-1). The column temperature was set at30 ℃,the detection wavelength was set at 325 nm,and the injection volume was 5 μL.HPLC characteristic fingerprints of Peucedani Radix,Peucedani Decursivi Radix and Peucedani Sub glabri Radix from different habitats were established,respectively.The obtained data were analyzed by Chempattern and Ezinfo chemometrics software.Results:PC A were used to analyze the LC-MS data,the results showed that Peucedani Radix,Peucedani Decursivi Radix and Peucedani Subglabri Radix clustered in different zones respectively,and chemical compositions in Peucedani Radix and its promiscuous herbs varied obviously.Conclusion:The proposed method is stable and reproducible,and the established characteristic fingerprint is specific,which can be used for identification and quality control of Peucedani Radix and its promiscuous herbs.
引文
[1]陶弘景.名医别录[M].北京:人民卫生出版社,1986TAO HJ.Mingyi Bielu[M].Beijing:People's Medical Publishing House,1986
[2]中华人民共和国药典2015年版.一部[S].2015:265ChP 2015.Vol I[S].2015:265
[3]中国科学院中国植物志编辑委员会.中国植物志.第55卷.第3分册[M].北京:科学出版社,1996:147Flora of China Editorial Committee.Flora Reipublicae Popularis Sinicae.55(3)[M].Beijing:Science Press,1996:147
[4]陈政雄,黄宝山,佘其龙,等.中药白花前胡化学成分的研究[J].药学学报,1979,14(8):486CHEN ZX,HUANG BS,SHE QL,et al. The chemical constituents of bai-hua-qian-hu,the root of Peucedanum praeruptorum Dunn(Umbelliferae)—four new coumarlns[J].Acta Phram Sin,1979,14(8):486
[5]KONG LY,LI Y,MIN ZD,et al.Coumarins from Peucedanum praeruptorum[J].Phytochemistry,1996,41(5):1423
[6]LU M,NICOLETTI M,BATTINELLI L,et al. Isolation of praeruptorins A and B from Peucedaum praeruptorum Dunn and their general pharmacological evaluation in comparison with extractsof the drug[J].IL Farmaco,2001,567(5-7):417
[7]LIU RM,FENG L,SUN AL,et al. Preparative isolation and purification of coumarins from Peucedanum praeruptorum Dunn by high-speed counter-current chromatography[J].J Chromatogr A,2004,1057(1-2):89
[8]ZHANG C,LI L,XIAO YQ,et al. A new phenanthraquinone from the roots of Peucedanum praeruptorum[J].Chin Chem Lett,2010,21(7):816
[9]甘肃省中药材标准2008年版[S].2008:119Standard for Chinese Medicinal Materials of Gansu Province 2008[S].2008:119
[10]陕西省药材标准2015年版[S].2015:176Standard for Chinese Medicinal Materials of Shaanxi Province 2015[S].2015:176
[11]四川省中药材标准1987年版[S].1987:47,50,61,98Standard for Chinese Medicinal Materials of Sichuan Province 1987[S].1987:47,50,61,98
[12]湖南省中药材标准2009年版[S].2009:226Standard for Chinese Medicinal Materials of Hunan Province[S].2009:226
[2]中华人民共和国药典2015年版.一部[S].2015:265ChP 2015.Vol I[S].2015:265
[3]中国科学院中国植物志编辑委员会.中国植物志.第55卷.第3分册[M].北京:科学出版社,1996:147Flora of China Editorial Committee.Flora Reipublicae Popularis Sinicae.55(3)[M].Beijing:Science Press,1996:147
[4]陈政雄,黄宝山,佘其龙,等.中药白花前胡化学成分的研究[J].药学学报,1979,14(8):486CHEN ZX,HUANG BS,SHE QL,et al. The chemical constituents of bai-hua-qian-hu,the root of Peucedanum praeruptorum Dunn(Umbelliferae)—four new coumarlns[J].Acta Phram Sin,1979,14(8):486
[5]KONG LY,LI Y,MIN ZD,et al.Coumarins from Peucedanum praeruptorum[J].Phytochemistry,1996,41(5):1423
[6]LU M,NICOLETTI M,BATTINELLI L,et al. Isolation of praeruptorins A and B from Peucedaum praeruptorum Dunn and their general pharmacological evaluation in comparison with extractsof the drug[J].IL Farmaco,2001,567(5-7):417
[7]LIU RM,FENG L,SUN AL,et al. Preparative isolation and purification of coumarins from Peucedanum praeruptorum Dunn by high-speed counter-current chromatography[J].J Chromatogr A,2004,1057(1-2):89
[8]ZHANG C,LI L,XIAO YQ,et al. A new phenanthraquinone from the roots of Peucedanum praeruptorum[J].Chin Chem Lett,2010,21(7):816
[9]甘肃省中药材标准2008年版[S].2008:119Standard for Chinese Medicinal Materials of Gansu Province 2008[S].2008:119
[10]陕西省药材标准2015年版[S].2015:176Standard for Chinese Medicinal Materials of Shaanxi Province 2015[S].2015:176
[11]四川省中药材标准1987年版[S].1987:47,50,61,98Standard for Chinese Medicinal Materials of Sichuan Province 1987[S].1987:47,50,61,98
[12]湖南省中药材标准2009年版[S].2009:226Standard for Chinese Medicinal Materials of Hunan Province[S].2009:226