美洲大蠊胸腺素THY3的表达模式分析
|
摘要
美洲大蠊Periplaneta americana胸腺素基因具有THY1、THY2和THY3三个不同剪接体,其中THY3结构域最多。本研究使用实时荧光定量PCR技术比较分析了THY3在美洲大蠊不同性别、不同发育阶段及不同组织中的表达差异,以及大肠杆菌Escherichia coli诱导对美洲大蠊血淋巴和脂肪体中THY3表达的影响。结果表明:THY3在成虫期的表达量显著高于其他虫期,雌虫表达量显著高于雄虫,脂肪体表达量显著高于血淋巴、头部、肌肉、体壁组织。雌性成虫体腔注射大肠杆菌3 h后血淋巴中THY3表达量显著增高,而在脂肪体中12 h后才检测到THY3表达明显上调,研究结果为进一步研究美洲大蠊胸腺素的功能打下了基础。
The thymosin gene of Periplaneta americana has 3 different spliceosomes including THY1, THY2, and THY3. THY3 possesses the largest number of structural domains. This study aims to figure out the expression difference of THY3 in different sexes, developmental stages and tissues of P. americana via real-time PCR. The effect of Escherichia coli-stimulation on THY3 expression was also analyzed. The results demonstrated that the expression of THY3 in adult P. americana was signifantly higher than any other stages. Female P. americana produced significantly higher amount of THY3 than male. Notably, fat had the highest THY3 expression level among the 5 tested tissues(fat, hemolymph, head, muscle and cuticle). Upon E. coli stimulation, the expression of THY3 in the hemolymph was significantly enhanced after 3 h, but increased in the fat after 12 h. These findings lay a solid foundation for further functional research of thymosin in P. americana.
The thymosin gene of Periplaneta americana has 3 different spliceosomes including THY1, THY2, and THY3. THY3 possesses the largest number of structural domains. This study aims to figure out the expression difference of THY3 in different sexes, developmental stages and tissues of P. americana via real-time PCR. The effect of Escherichia coli-stimulation on THY3 expression was also analyzed. The results demonstrated that the expression of THY3 in adult P. americana was signifantly higher than any other stages. Female P. americana produced significantly higher amount of THY3 than male. Notably, fat had the highest THY3 expression level among the 5 tested tissues(fat, hemolymph, head, muscle and cuticle). Upon E. coli stimulation, the expression of THY3 in the hemolymph was significantly enhanced after 3 h, but increased in the fat after 12 h. These findings lay a solid foundation for further functional research of thymosin in P. americana.
引文
初源,周帆,张明明,等.2013.昆虫天然免疫反应研究前沿[J].应用昆虫学报,50(2):311-320.
高洁,刘瑾华,孙晓红.等.2018.美洲大蠊精粉对小鼠血糖和肌酐的影响[J].四川动物,37(6):667-671.
耿华,安春菊,郝友进,等.2004.昆虫抗菌肽的结构和功能研究现状[J].生物学通报,39(10):1-4.
郭昕,巩荣艳,朱娟娟,等.2018.美洲大蠊乙醇提取物中原儿茶酸超高效液相色谱定量分析方法的建立[J].四川动物,37(2):185-190.
何娟娟,马列婷.2012.胸腺素β4研究进展[J].现代检验医学杂志,27(1):1-4.
何文耀,范嗣刚,刘宝锁,等.2018.合浦珠母贝胸腺素β4插核损伤和发育时期的表达研究[J].南方水产科学,14:66-74.
晋家正,李午佼,牟必琴,等.2018.药用美洲大蠊全基因组测序分析[J].四川动物,37(2):121-126.
康志琼,郝丽娟,马上上,等.2015.无脊椎动物β-胸腺素的功能研究进展[J].生物学杂志,32(2):76-79.
刘羽佳,李集临.2010.胸腺素β4研究进展[J].安徽农学通报,16:38-44.
刘玉媛.2006.天然药物康复新液的临床应用[J].天津药学,18(3):55-57.
史艳霞,李庆荣,黄志君,等.2009.昆虫变态发育过程中的细胞自噬和凋亡[J].昆虫学报,52(1):84-94.
肖小芹,汪世平,徐绍锐,等.2007.美洲大蠊提取物抗炎、镇痛作用的实验研究[J].中国病原生物学杂志,2(2):140-143.
赵双.2012.重组人胸腺素β4的表达纯化及生物学活性检测[D].北京:北京协和医学院.
周鑫鑫,王永娣,廖金旭,等.2018.家蚕胸腺素对蚕体抗核型多角体病毒感染能力的影响[J].蚕业科学,44(1):66-69.
Boquet I,Boujemaa R,Carlier MF,et al.2000.Ciboulot regulates actin assembly during Drosophila brain metamorphosis[J].Cell,102:797-808.
Cha H,Philp D,Lee SH,et al.2010.Over-expression of thymosin beta 4 promotes abnormal tooth development and stimulation of hair growth[J].International Journal of Developmental Biology,54:135-140.
Irobi E,Aguda AH,Larsson M,et al.2004.Structural basis of actin sequestration by thymosin-β4:implications for WH2 proteins[J].EMBO Journal,23(18):3599-3608.
Feng XW,Huo LJ,Yang MC,et al.2019.Thymosins participate in antibacterial immunity of kuruma shrimp,Marsupenaeus japonicas[J].Fish & Shellfish Immunology,84:244-251.
Freeman KW,Bowman BR,Zetter BR.2011.Regenerative protein thymosin beta-4 is a novel regulator of purinergic signaling[J].FASEB Journal,25:907-915.
Gai Y,Zhao J,Song L,et al.2009.Two thymosin-repeated molecules with structural and functional diversity coexist in Chinese mitten crab Eriocheir sinensis[J].Developmental & Comparative Immunology,33:867-876.
Girardi M,Sherling MA,Filler RB,et al.2003.Anti-inflammatory effects in the skin of thymosin-beta4 splice-variants[J].Immunology,109:1-7.
Goldstein AL,Slater FD,White A.1966.Preparation,assay,and partial purification of a thymic lymphocytopoietic factor (thymosin)[J].Proceedings of the National Academy of Sciences of the United States of America,56:1010-1017.
Hertzog M,Yarmola EG,Didry D,et al.2002.Control of actin dynamics by proteins made of beta-thymosin repeats:the actobindin family[J].Journal of Biological Chemistry,277:14786-14792.
Huff T,Mller CS,Otto AM,et al.2001.Beta-thymosins,small acidic peptides with multiple functions[J].International Journal of Biochemistry & Cell Biology,33:205-220.
Koshikawa S,Cornette R,Hojo M,et al.2005.Screening of genes expressed in developing mandibles during soldier differentiation in the termite Hodotermopsis sjostedti[J].FEBS Letters,579(6):1365-1370.
Lemaitre B,Hoffmann J.2007.The host defense of Drosophila melanogaster[J].Annual Review of Immunology,25:697-743.
Philp D,Goldstein AL,Kleinman HK.2004.Thymosin β4 promotes angiogenesis,wound healing,and hair follicle development[J].Mechanisms of Ageing & Development,125:113-115.
Saelee N,Noonin C,Nupan B,et al.2013.β-thymosins and hemocyte homeostasis in a crustacean[J].PLoS ONE,8:e60974.DOI:10.1371/journal.pone.0060974.
Troys MV,Dhaese S,Vandekerckhove J,et al.2007.Multirepeat β-Thymosins[M].New York:Springer.
Zhang FX,Shao HL,Wang JX,et al.2011.β-thymosin is upregulated by the steroid hormone 20-hydroxyecdysone and microorganisms[J].Insect Molecular Biology,20:519-527.
高洁,刘瑾华,孙晓红.等.2018.美洲大蠊精粉对小鼠血糖和肌酐的影响[J].四川动物,37(6):667-671.
耿华,安春菊,郝友进,等.2004.昆虫抗菌肽的结构和功能研究现状[J].生物学通报,39(10):1-4.
郭昕,巩荣艳,朱娟娟,等.2018.美洲大蠊乙醇提取物中原儿茶酸超高效液相色谱定量分析方法的建立[J].四川动物,37(2):185-190.
何娟娟,马列婷.2012.胸腺素β4研究进展[J].现代检验医学杂志,27(1):1-4.
何文耀,范嗣刚,刘宝锁,等.2018.合浦珠母贝胸腺素β4插核损伤和发育时期的表达研究[J].南方水产科学,14:66-74.
晋家正,李午佼,牟必琴,等.2018.药用美洲大蠊全基因组测序分析[J].四川动物,37(2):121-126.
康志琼,郝丽娟,马上上,等.2015.无脊椎动物β-胸腺素的功能研究进展[J].生物学杂志,32(2):76-79.
刘羽佳,李集临.2010.胸腺素β4研究进展[J].安徽农学通报,16:38-44.
刘玉媛.2006.天然药物康复新液的临床应用[J].天津药学,18(3):55-57.
史艳霞,李庆荣,黄志君,等.2009.昆虫变态发育过程中的细胞自噬和凋亡[J].昆虫学报,52(1):84-94.
肖小芹,汪世平,徐绍锐,等.2007.美洲大蠊提取物抗炎、镇痛作用的实验研究[J].中国病原生物学杂志,2(2):140-143.
赵双.2012.重组人胸腺素β4的表达纯化及生物学活性检测[D].北京:北京协和医学院.
周鑫鑫,王永娣,廖金旭,等.2018.家蚕胸腺素对蚕体抗核型多角体病毒感染能力的影响[J].蚕业科学,44(1):66-69.
Boquet I,Boujemaa R,Carlier MF,et al.2000.Ciboulot regulates actin assembly during Drosophila brain metamorphosis[J].Cell,102:797-808.
Cha H,Philp D,Lee SH,et al.2010.Over-expression of thymosin beta 4 promotes abnormal tooth development and stimulation of hair growth[J].International Journal of Developmental Biology,54:135-140.
Irobi E,Aguda AH,Larsson M,et al.2004.Structural basis of actin sequestration by thymosin-β4:implications for WH2 proteins[J].EMBO Journal,23(18):3599-3608.
Feng XW,Huo LJ,Yang MC,et al.2019.Thymosins participate in antibacterial immunity of kuruma shrimp,Marsupenaeus japonicas[J].Fish & Shellfish Immunology,84:244-251.
Freeman KW,Bowman BR,Zetter BR.2011.Regenerative protein thymosin beta-4 is a novel regulator of purinergic signaling[J].FASEB Journal,25:907-915.
Gai Y,Zhao J,Song L,et al.2009.Two thymosin-repeated molecules with structural and functional diversity coexist in Chinese mitten crab Eriocheir sinensis[J].Developmental & Comparative Immunology,33:867-876.
Girardi M,Sherling MA,Filler RB,et al.2003.Anti-inflammatory effects in the skin of thymosin-beta4 splice-variants[J].Immunology,109:1-7.
Goldstein AL,Slater FD,White A.1966.Preparation,assay,and partial purification of a thymic lymphocytopoietic factor (thymosin)[J].Proceedings of the National Academy of Sciences of the United States of America,56:1010-1017.
Hertzog M,Yarmola EG,Didry D,et al.2002.Control of actin dynamics by proteins made of beta-thymosin repeats:the actobindin family[J].Journal of Biological Chemistry,277:14786-14792.
Huff T,Mller CS,Otto AM,et al.2001.Beta-thymosins,small acidic peptides with multiple functions[J].International Journal of Biochemistry & Cell Biology,33:205-220.
Koshikawa S,Cornette R,Hojo M,et al.2005.Screening of genes expressed in developing mandibles during soldier differentiation in the termite Hodotermopsis sjostedti[J].FEBS Letters,579(6):1365-1370.
Lemaitre B,Hoffmann J.2007.The host defense of Drosophila melanogaster[J].Annual Review of Immunology,25:697-743.
Philp D,Goldstein AL,Kleinman HK.2004.Thymosin β4 promotes angiogenesis,wound healing,and hair follicle development[J].Mechanisms of Ageing & Development,125:113-115.
Saelee N,Noonin C,Nupan B,et al.2013.β-thymosins and hemocyte homeostasis in a crustacean[J].PLoS ONE,8:e60974.DOI:10.1371/journal.pone.0060974.
Troys MV,Dhaese S,Vandekerckhove J,et al.2007.Multirepeat β-Thymosins[M].New York:Springer.
Zhang FX,Shao HL,Wang JX,et al.2011.β-thymosin is upregulated by the steroid hormone 20-hydroxyecdysone and microorganisms[J].Insect Molecular Biology,20:519-527.