鲍鱼蛋白酶解物的抗氧化活性研究
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摘要
目的:研究鲍鱼肉不同蛋白酶解产物的抗氧化活性。方法:选择杂色鲍(Haliotis diversicolor),先以胰蛋白酶、木瓜蛋白酶和胰蛋白酶-木瓜蛋白酶组合对鲍鱼肉进行水解得到不同蛋白酶解物,然后以秀丽线虫(Caenorhabditis elegans)为模型,比较百草枯氧化胁迫条件下不同酶解物对秀丽线虫存活能力的影响。结果:胰蛋白酶和胰蛋白酶-木瓜蛋白酶组合酶解制备的鲍鱼肉蛋白酶解物得率较高;胰蛋白酶酶解物、木瓜蛋白酶酶解物和胰蛋白酶-木瓜蛋白酶组合酶解物都具有提高秀丽线虫氧化存活率的效果,但胰蛋白酶-木瓜蛋白酶组合酶解物效果最好。结论:综合考虑不同酶解产物的得率和抗氧化活性,胰蛋白酶-木瓜蛋白酶组合酶解的效果较好。
Objective: To study the antioxidant activityof peptide-rich abalone hydrolysates obtained with different proteases. Method: First prepare different abalone protein hydrolysatesby digesting the meat powder of the abalone Haliotis diversicolor using trypsin, papain or trypsin-papain combination and then test the antioxidant activity of the hydrolysates by determining the survival rate of the model animal Caenorhabditis elegans under paraquat-induced oxidative stress.Result: The abalone protein hydrolysate prepared by trypsin-papain combinatory digestion showed better antioxidant effect.Conclusion: Considering both yield and antioxidant capacity, the protein hydrolysate obtained by trypsin-papain digestion is the abalone preparation of choice for further study of abalone peptides.
Objective: To study the antioxidant activityof peptide-rich abalone hydrolysates obtained with different proteases. Method: First prepare different abalone protein hydrolysatesby digesting the meat powder of the abalone Haliotis diversicolor using trypsin, papain or trypsin-papain combination and then test the antioxidant activity of the hydrolysates by determining the survival rate of the model animal Caenorhabditis elegans under paraquat-induced oxidative stress.Result: The abalone protein hydrolysate prepared by trypsin-papain combinatory digestion showed better antioxidant effect.Conclusion: Considering both yield and antioxidant capacity, the protein hydrolysate obtained by trypsin-papain digestion is the abalone preparation of choice for further study of abalone peptides.
引文
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[3]Suleria HAR,Masci P,Zhao KN,et al.Anticoagulant and anti-thrombotic properties of blacklip abalone(Haliotis rubra):In Vitro and Animal Studies[J].Mar Drugs,2017,15(8):240.
[4]唐保军,王慧.我国鲍养殖研究现状及展望[J].渔业现代化,2009,36(1):30-34.
[5]Zhou DY,Zhu BW,Qiao L,et al.In vitro antioxidant activity of enzymatic hydrolysates prepared from abalone(Haliotis discus hannai Ino)viscera[J].Food Bioprod Process,2012,90(2):148-154.
[6]Je JY,Park SY,Wang JY,et al.Amino acid composition and in vitro antioxidant and cytoprotective activity of abalone viscera hydrolysate[J].J Funct Foods,2015(16):94-103.
[7]Zhou D Y,Tang Y,Zhu B W,et al.Antioxidant activity of hydrolysates obtained from scallop(Patinopecten yessoensis)and abalone(Haliotis discus hannai Ino)muscle[J].Food Chem,2012(132):815-822.
[8]Wang Q,Yang F,Guo W,et al.Caenorhabditis elegans in Chinese medicinal studies:making the case for aging and neurodegeneration[J].Rejuvenation Res,2014,17(2):205-208.
[9]Wang Q,Huang Y,Qin C,et al.Bioactive peptides from Angelica sinensis protein hydrolyzate delay senescence in Caenorhabditis elegans through antioxidant activities[J].Oxid Med Cell Longev,2016,2016:1-10.
[10]Han S K,Lee D,Lee H,et al.OASIS 2:online application for survival analysis 2with features for the analysis of maximal lifespan and healthspan in aging research[J].Oncotarget,2016,7(35):56147-56152.
[11]Zhang H,Pan N,Xiong S,et al.Inhibition of polyglutamine-mediated proteotoxicity by Astragalus membranaceus polysaccharide through the DAF-16/FOXO transcription factor in Caenorhabditis elegans[J].Biochem J,2012,441(1):417-424.
[12]Zhang J,Shi R,Li H,et al.Antioxidant and neuroprotective effects of Dictyophora indusiata polysaccharide in Caenorhabditis elegans[J].J Ethnopharmacol,2016(192):413-422.
[13]Jia W,Peng Q,Su L,et al.Novel bioactive peptides from Meretrix meretrix protect Caenorhabditis elegans against free radical-induced oxidative stress through the stress response factor DAF-16/FOXO[J].Mar Drugs,2018(16):E444.
[14]Yu X,Gao D,Xiao X,et al.Ophiopogon japonicus herbal tea ameliorates oxidative stress and extends lifespan in Caenorhabditis elegans[J].Pharmacogn Mag,2018(14):617-623.
[15]林端权,郭泽镔,张怡,等.海洋生物活性肽的研究进展[J].食品与工业科技,2016,37(18):367-373.
[16]Lee J K,Jeon J K,Byun H G.Antihypertensive effect of novel angiotensin I converting enzyme inhibitory peptide from chum salmon(Oncorhynchus keta)skin in spontaneously hypertensive rats[J].J Funct Foods,2014(7):381-389.
[17]Zhu C F,Li G Z,Peng H B,et al.Effect of marine collagen peptides on markers of metabolic nuclear receptors in type 2 diabetic patients with/without hypertension[J].Biomed Environ Sci,2010,23(2):113-120.