脂质组学在食品科学领域的研究现状与展望
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摘要
脂质是食品中基本营养成分之一,具有许多生物功能和营养学功能。脂质组学是研究脂质代谢的重要手段。随着色谱与质谱联用技术的发展,脂质分析手段不断完善,脂质组学也取得许多进展。本文主要从食品生物化学、食品营养学、食品质量安全控制学、食品加工学、食品贮藏等5个方面介绍脂质组学在食品科学领域中的研究现状,并探索脂质组学在食品领域中巨大的发展空间。
Lipid, which has many biological functions and nutritional functions, is one of the major food nutrients.Lipidomics is an important approach aiming at analysis of lipid metabolisms. With the development of chromatography combined with mass spectrometry, the method of lipid research is continuously improved, and lipidomics has got a lot progress. This paper mainly reviews the research status of lipidomics in food science from food biochemistry, food nutriology, food quality and safety, food processing and food storage. It also explores the huge development space of lipidomics in food field.
Lipid, which has many biological functions and nutritional functions, is one of the major food nutrients.Lipidomics is an important approach aiming at analysis of lipid metabolisms. With the development of chromatography combined with mass spectrometry, the method of lipid research is continuously improved, and lipidomics has got a lot progress. This paper mainly reviews the research status of lipidomics in food science from food biochemistry, food nutriology, food quality and safety, food processing and food storage. It also explores the huge development space of lipidomics in food field.
引文
[1] NICHOLSON J K, WILSON I D. Understanding‘Global’ systembiology:metabonomics and the continuum of metabolism[J]. Nature Review Drug Discovery, 2003, 2(4):668-677.
[2]王龑,许文涛,赵维薇,等.组学技术及其在食品科学中应用的研究进展[J].生物技术通报, 2011(11):26-32.
[3] HAN X L, GROSS R W. Global analyses of cellular lipidomes directly from crude extracts of biological samples by ESI mass spectrometry:a bridge to lipidomics[J]. Journal of Lipid Research, 2003, 44(6):1071-1079.
[4] FAHY E, SUBRAMANIAM S, BROWN H A, et al. A comprehensive classification system for lipids[J].Journal of Lipid Research, 2005, 46(5):839-862.
[5]李幼生,黎介寿.营养支持中的物质代谢与营养代谢组学[J].肠外与肠内营养, 2006, 13(6):321-323.
[6] YETUKURI L, KATAJAMAA M, MEDINA-GOMEZ G, et al. Bioinformatics strategies for lipidomics analysis:characterization of obesity related hepatic steatosis[J]. BMC Systems Biology, 2007, 1(1):12.
[7] GOLDBERG I J. Lipoprotein lipase and lipolysis:central roles in lipoprotein metabolism and therogenesis[J]. Journal of Lipid Research, 1996, 37(4):693-707.
[8] LIBBY P, THEROUR P. Pathophysiology of coronary artery disease[J]. Circulation, 2005, 111(25):3481-3488.
[9] WENK M R. The emerging field of lipidomics[J].Nature Reviews Drug Discovery, 2005, 4(7):594-610.
[10] MORALES A, LEE H, GONI F M, et al. Sphingolipids and cell death[J]. Apoptosis, 2007, 12(5):923-939.
[11] VAN MEER G. Cellular lipidomics[J]. Embo Journal,2005, 24(18):3159-3165.
[12] RAPPLEY I, MYERS D S, MILNE S B, et al.Lipidomic profiling in mouse brain reveals differences between ages and genders with smaller changes associated withα-synuclein genotype[J].Journal of Neurochemistry, 2009, 111(1):15-25.
[13] ADIBHATL R M, HATCHER J F, DEMPSEY R J.Lipids and lipidomics in brain injury and diseases[J].Aaps Journal, 2006, 8(2):314-321.
[14] GORDEN D L, IVANOVA P T, MYERS D S, et al. Increased diacylglycerol characterize hepatic lipid changes in progression of human nonalcoholic fatty liver disease; comparison to a murine model[J].PLoS One, 2011, 6(8):e22775.
[15]朱超,胡坪,梁琼麟,等.脂组学及其研究进展[J].分析化学, 2009, 37(9):1390-1396.
[16]蔡潭溪,刘平生,杨福全,等.脂质组学研究进展[J].生物化学与生物物理进展, 2010, 37(2):121-128.
[17]蔡教英,欧阳克蕙,上官新晨,等.脂质代谢组学的研究进展[J].动物营养学报, 2011, 23(11):1870-1876.
[18]陈宇欢,李静,范亚苇,等.脂质组学及其在营养与健康研究中的应用研究进展[J].食品科学, 2014,35(15):272-276.
[19]王涛,钟秀丽,梅旭荣,等.基于电喷雾电离串联质谱技术的植物脂质组学研究进展[J].生物化学与生物物理进展, 2010, 37(10):1074-1081.
[20]王涛,梅旭荣,钟秀丽,等.脂质组学研究方法及其应用[J].植物学报, 2010, 45(2):249-257.
[21]陆姝欢,杨松,元英进.脂质组学在医药研究中的应用[J].细胞生物学杂志, 2007, 29(2):169-172.
[22] JIN R, LI L, FENG J, et al. Zwitterionic hydrophilic interaction solid-phase extraction and multi-dimensional mass spectrometry for shotgun lipidomic study of Hypophthalmichthys nobilis[J]. Food Chemistry, 2016, 216:347-354.
[23]杨新泉,江正强,杜生明,等.我国食品科学学科的历史、现状和发展方向[J].中国食品学报, 2010,10(5):5-13.
[24] CAMPOS P S, QUARTIN V, RAMALHO J C, et al. Electrolyte leakage and lipid degradation account for cold sensitivity in leaves of Coffea sp. plants[J].Journal of Plant Physiology, 2003, 160(3):283-292.
[25] WELTI R, LI W Q, LI M Y, et al. Profiling membrane lipids in plant stress responses. Role of phospholipase Dαin freezing-induced lipid changes in Arabidopsi s[J]. Journal of Biological Chemistry,2002, 227(35):31994-32002.
[26] LI M Y, WELTI R, WANG X M. Quantitative profiling of Arabidopsis polar glycerolipids in response to phosphate starvation. Roles of PLDζ1 and PLDζ2in phosphatidylcholine hydrolysis and digalactosyldiacylglycerol accumulation in phosphate-starved root[J].Plant Physiol, 2006, 142(2):750-761.
[27] ZHANG Y Y, ZHU H Y, ZHANG Q, et al. Phospholipase Dα1 and phosphatidic acid regulate NADPH oxidase activity and production of reactive oxygen species in ABA-mediated stomatal closure in Arabidopsis[J]. Plant Cell, 2009, 21(8):2357-2377.
[28] DEVAIAH S P, ROTH M R, BAUGHMAN E, et al. Quantitative profiling of polar glycerolipid species from organs of wild-type Arabidopsis and a phospholipase Dalpha1 knockout mutant[J]. Phytochemistry, 2006, 67(17):1907-1924.
[29] DEVAIAH S P, PAN X Q, HONG Y Y, et al.Enhancing seed quality and viability by suppressing phospholipase D in Arabidopsis[J]. Plant Journal,2007, 50(6):950-957.
[30] HONG Y Y, DEVAIAH S P, BAHN S C, et al.Phospholipase Dεand phosphatidic acid enhance Arabidopsis nitrogen signaling and growth[J]. Plant Journal, 2009, 58(3):376-387.
[31]王友谊,张虹,戴志远.直接进样电喷雾串联质谱法测定草鱼肌肉组织中磷脂[J].分析化学, 2012,40(6):893-898.
[32] SHEN Q, WANG Y, GONG L, et al. Shotgun lipidomics strategy for fast analysis of phospholipids in fisheries waste and its potential in species differentiation[J]. Journal of Agricultural&Food Chemistry, 2012, 60(37), 9384-9393.
[33]饶平凡,刘树滔,周建武,等.食品科学还必须研究什么?[J].食品科学技术学报, 2015, 33(3):1-4.
[34]张双庆,黄振武,杨月欣.食物组学——现代食物科学和营养学研究的新方法[J].卫生研究, 2014,43(5):881-885.
[35] PUIGGROS F, SOLA R, BLADE C, et al. Nutritional biomarkers and foodomic methodologies for qualitative and quantitative analysis of bioactive ingredients in dietary intervention studies[J]. Journal of Chromatography A, 2011,1218(42):7399-7414.
[36] SHEN Q, YANG M, LI L, et al. Graphene/TiO2nanocomposite based solid-phase extraction and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for lipidomic profiling of avocado(Persea americana Mill.)[J]. Analytica Chimica Acta,2014, 852:153-161.
[37] SHEN Q, DONG W, YANG M, et al. Lipidomic fingerprint of almonds(Prunus dulcis L. cv Nonpareil)using TiO2nanoparticle based matrix solidphase dispersion and MALDI-TOF/MS and its potential in geographical origin verification[J]. Journal of Agricultural&Food Chemistry, 2013, 61(32):7739-7748.
[38] SHEN Q, DONG W, YANG M, et al. Lipidomic study of olive fruit and oil using TiO2nanoparticle based matrix solid-phase dispersion and MALDITOF/MS[J]. Food Research International, 2013, 54(2):2054-2061.
[39] SHEN Q, CHEUNG H Y. A TiO2/SiO2core-shell composites based sample preparation method for selective extraction of phospholipids from shrimp waste followed by HILIC-QTOF/MS analysis[J]. Journal of Agricultural&Food Chemistry, 2014, 62(36):8944-8951.
[40] LAMAZIERE A, WOLF C, BARBE U, et al.Lipidomics of hepatic lipogenesis inhibition by omega 3 fatty acids[J]. Prostaglandins, Leukotrienes and Essential Fatty Acids, 2013, 88(2):149-154.
[41] PIETIL魧INEN K H, SYSI-AHO M, ORESIC M, et al. Acquired obesity is associated with changes in the serum lipidomic profile independent of genetic effects——a monozygotic twin study[J]. PLoS One,2007, 2(2):e218.
[42]周蕴华,尹慧勇.质谱技术在现代营养学和食品分析检测中的应用[J].生命科学, 2013, 25(2):240-247.
[43] WANG C, KONG H, GUAN Y, et al. Plasma phospholipid metabolic profiling and biomarkers of type 2 diabetes mellitus based on high-performance liquid chromatography/electrospray mass spectrometry and multivariate statistical analysis[J]. Analytical Chemistry, 2005, 77(13):4108-4116.
[44]李国琛,王颜红,吴仁安,等.基于MALDI-TOFMS的脂质组学研究——鲫鱼受镉暴露的潜在生物标志物[J].高等学校化学学报, 2010, 31(2):269-273.
[45] NOUALRI I, GHNAYAB T, YOUSSEFA N B, et al. Changes in content and fatty acid profiles of total lipids of two halophytes:Sesuvium portula-castrum and Mesembryanthemum crystallinum under cadmium stress[J]. Journal of Plant Physiology, 2006,163(11):1198-1202.
[46]汤丹剑.气相色谱鉴别食品微生物的初步研究[J].宁波大学学报, 1997, 10(3):14-20.
[47] HOFFMANN M, KEYS C E, SONG K Y, et al.Evaluation of multiple strains of Enterobacter sakazakii using fatty acid profiles[J]. Food Chemistry, 2008,107(4):1623-1628.
[48]赵金松,郑佳,吴重德,等.基于磷脂脂肪酸技术研究酱香大曲微生物群落结构[J].应用与环境生物学报, 2014, 20(4):558-563.
[49] SHEN Q, YANG Q, CHEUNG H Y. Hydrophilic interaction chromatography based solid-phase extraction and MALDI TOF mass spectrometry for revealing the influence of pseudomonas fluorescens on phospholipids in salmon fillet[J]. Analytical&Bioanalytical Chemistry, 2015, 407(5):1475-1484.
[50] FAUCONNIER M L, WELTI R, BLEE E, et al.Lipid and oxylipin profiles during aging and sprout development in potato tubers(Solanum tuberosum L.)[J]. Biochimica Et Biophysica Acta, 2003, 1633(2):118-126.
[2]王龑,许文涛,赵维薇,等.组学技术及其在食品科学中应用的研究进展[J].生物技术通报, 2011(11):26-32.
[3] HAN X L, GROSS R W. Global analyses of cellular lipidomes directly from crude extracts of biological samples by ESI mass spectrometry:a bridge to lipidomics[J]. Journal of Lipid Research, 2003, 44(6):1071-1079.
[4] FAHY E, SUBRAMANIAM S, BROWN H A, et al. A comprehensive classification system for lipids[J].Journal of Lipid Research, 2005, 46(5):839-862.
[5]李幼生,黎介寿.营养支持中的物质代谢与营养代谢组学[J].肠外与肠内营养, 2006, 13(6):321-323.
[6] YETUKURI L, KATAJAMAA M, MEDINA-GOMEZ G, et al. Bioinformatics strategies for lipidomics analysis:characterization of obesity related hepatic steatosis[J]. BMC Systems Biology, 2007, 1(1):12.
[7] GOLDBERG I J. Lipoprotein lipase and lipolysis:central roles in lipoprotein metabolism and therogenesis[J]. Journal of Lipid Research, 1996, 37(4):693-707.
[8] LIBBY P, THEROUR P. Pathophysiology of coronary artery disease[J]. Circulation, 2005, 111(25):3481-3488.
[9] WENK M R. The emerging field of lipidomics[J].Nature Reviews Drug Discovery, 2005, 4(7):594-610.
[10] MORALES A, LEE H, GONI F M, et al. Sphingolipids and cell death[J]. Apoptosis, 2007, 12(5):923-939.
[11] VAN MEER G. Cellular lipidomics[J]. Embo Journal,2005, 24(18):3159-3165.
[12] RAPPLEY I, MYERS D S, MILNE S B, et al.Lipidomic profiling in mouse brain reveals differences between ages and genders with smaller changes associated withα-synuclein genotype[J].Journal of Neurochemistry, 2009, 111(1):15-25.
[13] ADIBHATL R M, HATCHER J F, DEMPSEY R J.Lipids and lipidomics in brain injury and diseases[J].Aaps Journal, 2006, 8(2):314-321.
[14] GORDEN D L, IVANOVA P T, MYERS D S, et al. Increased diacylglycerol characterize hepatic lipid changes in progression of human nonalcoholic fatty liver disease; comparison to a murine model[J].PLoS One, 2011, 6(8):e22775.
[15]朱超,胡坪,梁琼麟,等.脂组学及其研究进展[J].分析化学, 2009, 37(9):1390-1396.
[16]蔡潭溪,刘平生,杨福全,等.脂质组学研究进展[J].生物化学与生物物理进展, 2010, 37(2):121-128.
[17]蔡教英,欧阳克蕙,上官新晨,等.脂质代谢组学的研究进展[J].动物营养学报, 2011, 23(11):1870-1876.
[18]陈宇欢,李静,范亚苇,等.脂质组学及其在营养与健康研究中的应用研究进展[J].食品科学, 2014,35(15):272-276.
[19]王涛,钟秀丽,梅旭荣,等.基于电喷雾电离串联质谱技术的植物脂质组学研究进展[J].生物化学与生物物理进展, 2010, 37(10):1074-1081.
[20]王涛,梅旭荣,钟秀丽,等.脂质组学研究方法及其应用[J].植物学报, 2010, 45(2):249-257.
[21]陆姝欢,杨松,元英进.脂质组学在医药研究中的应用[J].细胞生物学杂志, 2007, 29(2):169-172.
[22] JIN R, LI L, FENG J, et al. Zwitterionic hydrophilic interaction solid-phase extraction and multi-dimensional mass spectrometry for shotgun lipidomic study of Hypophthalmichthys nobilis[J]. Food Chemistry, 2016, 216:347-354.
[23]杨新泉,江正强,杜生明,等.我国食品科学学科的历史、现状和发展方向[J].中国食品学报, 2010,10(5):5-13.
[24] CAMPOS P S, QUARTIN V, RAMALHO J C, et al. Electrolyte leakage and lipid degradation account for cold sensitivity in leaves of Coffea sp. plants[J].Journal of Plant Physiology, 2003, 160(3):283-292.
[25] WELTI R, LI W Q, LI M Y, et al. Profiling membrane lipids in plant stress responses. Role of phospholipase Dαin freezing-induced lipid changes in Arabidopsi s[J]. Journal of Biological Chemistry,2002, 227(35):31994-32002.
[26] LI M Y, WELTI R, WANG X M. Quantitative profiling of Arabidopsis polar glycerolipids in response to phosphate starvation. Roles of PLDζ1 and PLDζ2in phosphatidylcholine hydrolysis and digalactosyldiacylglycerol accumulation in phosphate-starved root[J].Plant Physiol, 2006, 142(2):750-761.
[27] ZHANG Y Y, ZHU H Y, ZHANG Q, et al. Phospholipase Dα1 and phosphatidic acid regulate NADPH oxidase activity and production of reactive oxygen species in ABA-mediated stomatal closure in Arabidopsis[J]. Plant Cell, 2009, 21(8):2357-2377.
[28] DEVAIAH S P, ROTH M R, BAUGHMAN E, et al. Quantitative profiling of polar glycerolipid species from organs of wild-type Arabidopsis and a phospholipase Dalpha1 knockout mutant[J]. Phytochemistry, 2006, 67(17):1907-1924.
[29] DEVAIAH S P, PAN X Q, HONG Y Y, et al.Enhancing seed quality and viability by suppressing phospholipase D in Arabidopsis[J]. Plant Journal,2007, 50(6):950-957.
[30] HONG Y Y, DEVAIAH S P, BAHN S C, et al.Phospholipase Dεand phosphatidic acid enhance Arabidopsis nitrogen signaling and growth[J]. Plant Journal, 2009, 58(3):376-387.
[31]王友谊,张虹,戴志远.直接进样电喷雾串联质谱法测定草鱼肌肉组织中磷脂[J].分析化学, 2012,40(6):893-898.
[32] SHEN Q, WANG Y, GONG L, et al. Shotgun lipidomics strategy for fast analysis of phospholipids in fisheries waste and its potential in species differentiation[J]. Journal of Agricultural&Food Chemistry, 2012, 60(37), 9384-9393.
[33]饶平凡,刘树滔,周建武,等.食品科学还必须研究什么?[J].食品科学技术学报, 2015, 33(3):1-4.
[34]张双庆,黄振武,杨月欣.食物组学——现代食物科学和营养学研究的新方法[J].卫生研究, 2014,43(5):881-885.
[35] PUIGGROS F, SOLA R, BLADE C, et al. Nutritional biomarkers and foodomic methodologies for qualitative and quantitative analysis of bioactive ingredients in dietary intervention studies[J]. Journal of Chromatography A, 2011,1218(42):7399-7414.
[36] SHEN Q, YANG M, LI L, et al. Graphene/TiO2nanocomposite based solid-phase extraction and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for lipidomic profiling of avocado(Persea americana Mill.)[J]. Analytica Chimica Acta,2014, 852:153-161.
[37] SHEN Q, DONG W, YANG M, et al. Lipidomic fingerprint of almonds(Prunus dulcis L. cv Nonpareil)using TiO2nanoparticle based matrix solidphase dispersion and MALDI-TOF/MS and its potential in geographical origin verification[J]. Journal of Agricultural&Food Chemistry, 2013, 61(32):7739-7748.
[38] SHEN Q, DONG W, YANG M, et al. Lipidomic study of olive fruit and oil using TiO2nanoparticle based matrix solid-phase dispersion and MALDITOF/MS[J]. Food Research International, 2013, 54(2):2054-2061.
[39] SHEN Q, CHEUNG H Y. A TiO2/SiO2core-shell composites based sample preparation method for selective extraction of phospholipids from shrimp waste followed by HILIC-QTOF/MS analysis[J]. Journal of Agricultural&Food Chemistry, 2014, 62(36):8944-8951.
[40] LAMAZIERE A, WOLF C, BARBE U, et al.Lipidomics of hepatic lipogenesis inhibition by omega 3 fatty acids[J]. Prostaglandins, Leukotrienes and Essential Fatty Acids, 2013, 88(2):149-154.
[41] PIETIL魧INEN K H, SYSI-AHO M, ORESIC M, et al. Acquired obesity is associated with changes in the serum lipidomic profile independent of genetic effects——a monozygotic twin study[J]. PLoS One,2007, 2(2):e218.
[42]周蕴华,尹慧勇.质谱技术在现代营养学和食品分析检测中的应用[J].生命科学, 2013, 25(2):240-247.
[43] WANG C, KONG H, GUAN Y, et al. Plasma phospholipid metabolic profiling and biomarkers of type 2 diabetes mellitus based on high-performance liquid chromatography/electrospray mass spectrometry and multivariate statistical analysis[J]. Analytical Chemistry, 2005, 77(13):4108-4116.
[44]李国琛,王颜红,吴仁安,等.基于MALDI-TOFMS的脂质组学研究——鲫鱼受镉暴露的潜在生物标志物[J].高等学校化学学报, 2010, 31(2):269-273.
[45] NOUALRI I, GHNAYAB T, YOUSSEFA N B, et al. Changes in content and fatty acid profiles of total lipids of two halophytes:Sesuvium portula-castrum and Mesembryanthemum crystallinum under cadmium stress[J]. Journal of Plant Physiology, 2006,163(11):1198-1202.
[46]汤丹剑.气相色谱鉴别食品微生物的初步研究[J].宁波大学学报, 1997, 10(3):14-20.
[47] HOFFMANN M, KEYS C E, SONG K Y, et al.Evaluation of multiple strains of Enterobacter sakazakii using fatty acid profiles[J]. Food Chemistry, 2008,107(4):1623-1628.
[48]赵金松,郑佳,吴重德,等.基于磷脂脂肪酸技术研究酱香大曲微生物群落结构[J].应用与环境生物学报, 2014, 20(4):558-563.
[49] SHEN Q, YANG Q, CHEUNG H Y. Hydrophilic interaction chromatography based solid-phase extraction and MALDI TOF mass spectrometry for revealing the influence of pseudomonas fluorescens on phospholipids in salmon fillet[J]. Analytical&Bioanalytical Chemistry, 2015, 407(5):1475-1484.
[50] FAUCONNIER M L, WELTI R, BLEE E, et al.Lipid and oxylipin profiles during aging and sprout development in potato tubers(Solanum tuberosum L.)[J]. Biochimica Et Biophysica Acta, 2003, 1633(2):118-126.