长链非编码RNA NNT-AS1促进人肺癌细胞增殖并抑制细胞凋亡的研究
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摘要
目的研究长链非编码RNA NNT-AS1在人肺癌组织及细胞中的表达和作用。方法收集人肺癌组织和五种不同的肺癌细胞株,进行qPCR分析,检测肺癌组织、细胞中NNT-AS1的相对转录水平。研究NNT-AS1在肺癌细胞集落形成、细胞增殖和细胞凋亡中的作用。结果①人肺癌组织及细胞株中LncRNA NNT-AS1过度表达。LncRNA NNT-AS1表达与肿瘤大小、TNM分期呈正相关(P=0.005,P=0.042)。②NNT-AS1敲低抑制A549和95D细胞的集落形成能力。③A549和95D细胞中的NNT-AS1下调可抑制细胞增殖。④A549和95D细胞中的NNT-AS1下调可以促进细胞凋亡。结论 LncRNA NNT-AS1促进人肺癌细胞增殖并抑制细胞凋亡,是肺癌的致癌因子。NNT-AS1是肺癌诊断和治疗的新型lncRNA,为肺癌的诊断和治疗提供了新的线索。
Objective To study the expression and function of long non-coding RNA NNT-AS1 in human lung cancer tissues and cells.Methods Human lung cancer tissues and five different lung cancer cell lines were collected. QPCR analysis was performed to detect the relative transcriptional level of NNT-AS1 in lung cancer tissues and cells. To study the role of NNT-AS1 in the colony formation, cell proliferation and apoptosis of lung cancer cells.Results ①LncRNA NNT-AS1 was overexpressed in human lung cancer tissues and cell lines. The expression of LncRNA NNT-AS1 was positively correlated with tumor size and TNM stage(P=0.005, P=0.042). ②NNT-AS1 knockdown inhibits colony forming ability of A549 and 95 D cells.③The down-regulation of NNT-AS1 in A549 and 95 D cells could inhibit cell proliferation. ④The down-regulation of NNT-AS1 in A549 and 95 D cells can promote apoptosis.Conclusion LncRNA NNT-AS1 promotes the proliferation of human lung cancer cells and inhibits cell apoptosis, which is a carcinogenic factor of lung cancer. NNT-AS1 is a new lncRNA for the diagnosis and treatment of lung cancer. It provides new clues for the diagnosis and treatment of lung cancer.
Objective To study the expression and function of long non-coding RNA NNT-AS1 in human lung cancer tissues and cells.Methods Human lung cancer tissues and five different lung cancer cell lines were collected. QPCR analysis was performed to detect the relative transcriptional level of NNT-AS1 in lung cancer tissues and cells. To study the role of NNT-AS1 in the colony formation, cell proliferation and apoptosis of lung cancer cells.Results ①LncRNA NNT-AS1 was overexpressed in human lung cancer tissues and cell lines. The expression of LncRNA NNT-AS1 was positively correlated with tumor size and TNM stage(P=0.005, P=0.042). ②NNT-AS1 knockdown inhibits colony forming ability of A549 and 95 D cells.③The down-regulation of NNT-AS1 in A549 and 95 D cells could inhibit cell proliferation. ④The down-regulation of NNT-AS1 in A549 and 95 D cells can promote apoptosis.Conclusion LncRNA NNT-AS1 promotes the proliferation of human lung cancer cells and inhibits cell apoptosis, which is a carcinogenic factor of lung cancer. NNT-AS1 is a new lncRNA for the diagnosis and treatment of lung cancer. It provides new clues for the diagnosis and treatment of lung cancer.
引文
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[3] Goff LA ,Rinn JL . Linking RNA biology to lncRNAs[J]. Genome Res, 2015, 25(10): 1456-65.
[4] Chau YM, Pando S ,Taylor HS .HOXA11 silencing and endogenous HOXA11 antisense ribonucleic acid in the uterine endometrium[J].J Clin Endocrinol Metab, 2002, 87(6): 2674-80.
[5] Liu B,Pan CF,He ZC, et al. Long Noncoding RNA-LET Suppresses Tumor Growth and EMT in Lung Adenocarcinoma[J]. Biomed Res Int, 2016, 2016: 4693471.
[6] Zhu H,Zhang L,Yan S,et al. LncRNA16 is a potential biomarker for diagnosis of early-stage lung cancer that promotes cell proliferation by regulating the cell cycle[J]. Oncotarget, 2017,8(5):7867-77.
[7] Wang Q,Yang L,Hu X,et al. Upregulated NNT-AS1, a long noncoding RNA, contributes to proliferation and migration of colorectal cancer cells in vitro and in vivo[J]. Oncotarget, 2017,8(2):3441-53.
[8] Zheng QW,Zhou YL,You QJ, et al. WWOX inhibits the invasion of lung cancer cells by downregulating RUNX2[J]. Cancer Gene Ther, 2016,23(12):433-38.
[9] Thedieck K, Holzwarth B, Prentzell MT,et al. Inhibition of mTORC1 by astrin and stress granules prevents apoptosis in cancer cells[J]. Cell, 2013, 154(4): 859-74.