高温酸解辅助酶解猪毛工艺的研究
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摘要
为了提高猪毛中角蛋白的加工利用,探讨了高温酸解辅助酶解猪毛的工艺条件。以猪毛溶解率和可溶性蛋白质含量为指标,通过单因素试验、正交试验和响应面试验优化猪毛溶解工艺。结果表明:高温酸解处理最优条件为温度125℃、处理时间45 min、盐酸浓度0.25 mol/L,猪毛的溶解率达58.21%;酶解工艺的最优条件为酸解液调整pH值为7.4、复合蛋白酶加酶量为3.4%、酶解时间为10.9 h,猪毛溶解率达86.33%,可溶性蛋白质含量达312.397 mg/g。采用先高温酸解再酶解的方法不仅降低了酸使用量,相比较只采用高温酸解处理猪毛溶解率提高了48.31%。
In order to improve the processing and utilization of keratin in hog hair,the process conditions of high temperature acid hydrolysis assisted with enzymatic hydrolysis of hog hair were discussed.The hog hair dissolution rate and soluble protein content were used as indicators to optimize the hog hair dissolution process by single factor test,orthogonal test and response surface experiment.The results showed that the optimal conditions for high temperature acid hydrolysis were 125℃,45 min,and 0.25 mol·L~(-1) hydrochloric acid.The dissolution rate of hog hair was 58.21%.The optimal conditions for the enzymatic hydrolysis process are pH adjustment of the acid solution to 7.4,the alkaline protease amount was 3.5%,and the enzymatic hydrolysis time was 10.9 h.At this time,the dissolution rate of hog hair could reach 86.33% and the soluble protein content reached 312.397 mg/g.The dissolution rate of the high-temperature acid hydrolysisassistedwithenzymatic hydrolysis was 48.31% higher than that of the high-temperature acid hydrolysis.
In order to improve the processing and utilization of keratin in hog hair,the process conditions of high temperature acid hydrolysis assisted with enzymatic hydrolysis of hog hair were discussed.The hog hair dissolution rate and soluble protein content were used as indicators to optimize the hog hair dissolution process by single factor test,orthogonal test and response surface experiment.The results showed that the optimal conditions for high temperature acid hydrolysis were 125℃,45 min,and 0.25 mol·L~(-1) hydrochloric acid.The dissolution rate of hog hair was 58.21%.The optimal conditions for the enzymatic hydrolysis process are pH adjustment of the acid solution to 7.4,the alkaline protease amount was 3.5%,and the enzymatic hydrolysis time was 10.9 h.At this time,the dissolution rate of hog hair could reach 86.33% and the soluble protein content reached 312.397 mg/g.The dissolution rate of the high-temperature acid hydrolysisassistedwithenzymatic hydrolysis was 48.31% higher than that of the high-temperature acid hydrolysis.
引文
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[3] 吴美芳,于世国,张文昭.猪毛下脚的综合利用[J].化工时刊,1990(4):17-21.
[4] 董敏玉,王海滨,范海涛,等.猪毛水解蛋白粉的研制及其营养价值评价[J].肉类工业,1992(10):37-41.
[5] JOU C J G,CHEN Y S,WANG H P,et al.Hydrolytic dissociation of hog-hair by microwave radiation[J].Bioresource Technology,1999,70(1):111-113.
[6] 吕全建,王建玲.毛发水解液用作动物饲料蛋白质的研究[J].河南理工大学学报(自然科学版),2000,19(5):393-395.
[7] 郭思亚,向鹏妍,吴婷,等.酶辅助碱解猪毛提取角蛋白工艺优化[J].农产品加工,2017(8):19-23.
[8] 柳荫,吴凤智,陈龙,等.考马斯亮蓝法测定核桃水溶性蛋白含量的研究[J].中国酿造,2013,32(12):131-133.
[9] 邓丽莉,潘晓倩,生吉萍,等.考马斯亮蓝法测定苹果组织微量可溶性蛋白含量的条件优化[J].食品科学,2012,33(24):185-189.
[10] 何春林.利用低值鱼制备高吸水剂及其应用研究[D].广州:广东工业大学,2009.
[11] 施瑛,裴斐,周玲玉,等.响应面法优化复合酶法提取紫菜藻红蛋白工艺[J].食品科学,2015,36(6):51-57.
[12] 张泽志,韩春亮,李成未.响应面法在试验设计与优化中的应用[J].河南教育学院学报(自然科学版),2011,20(4):34-37.
[13] LIU Yong,GONG Guili,ZHANG Jiao,et al.Response surface optimization of ultrasound-assisted enzymatic extractionpolysaccharides from Lycium barbarum[J].Carbohydrate Polymers,2014,110:278-284.
[14] IMEN E,WAFA H,MANEL B T,et al.Development and optimisation of a non conventional extraction process of natural dye from olive solid waste using response surface methodology (RSM)[J].Food Chemistry,2014,161:345-352.
[15] JIA Junqiang,MA Haile,ZHAO Weirui,et al.The use of ultrasound for enzymatic preparation of ACE inhibitory peptides from wheat germ protein[J].Food Chemistry,2010,119(1):336-342.
[16] 蒋少龙,蔡俊.角蛋白酶研究及应用进展[J/OL].食品工业科技:1-11[2019-01-15].