铁皮石斛对大鼠血液生化指标影响的研究
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摘要
目的探讨铁皮石斛对大鼠血液生化指标的影响。方法采用拌饲法连续喂养大鼠90 d后,观察试验动物一般生长情况,测试谷草转氨酶、谷丙转氨酶、胆固醇、甘油三酯等血生化指标。结果各剂量组大鼠体重、增重、进食量及食物利用率均正常。大鼠谷丙转氨酶出现降低,雄性大鼠受试组与对照组比较有统计学差异(P<0.01)。中、高剂量组雄性大鼠胆固醇降低,与对照组比较,在统计学上有显著性差异(P<0.01);与对照组比较,低、中剂量组雌性大鼠胆固醇出现降低,在统计学上有显著性差异(P<0.05)。结论铁皮石斛具有降低受试动物大鼠谷丙转氨酶和胆固醇的作用。
Objective To investigate the effects of Dendrobium officinale on blood biochemical indexes of rats.Methods After the rats were continuously fed with mixed feeding method for 90 d, the general growth conditions of the experimental animals were observed, and blood biochemical indexes such as glutamic oxaloacetic transaminase(AST), alanine transaminase(ALT), cholesterol(TC) and triglyceride(TG) were tested. Results The body weight,weight gain, food intake and food utilization rate of rats in each dose group were normal. The results showed that the ALT in male rats decreased and there was a significant difference between the experimental group and the control group(P<0.01). Compared with the control group, the TC of male rats in the middle and high dose groups decreased significantly(P<0.01). Compared with the control group, the TC of female rats in the low and middle dose groups decreased significantly(P<0.05). Conclusion Dendrobium officinale has the effect of reducing ALT and TC in rats.
Objective To investigate the effects of Dendrobium officinale on blood biochemical indexes of rats.Methods After the rats were continuously fed with mixed feeding method for 90 d, the general growth conditions of the experimental animals were observed, and blood biochemical indexes such as glutamic oxaloacetic transaminase(AST), alanine transaminase(ALT), cholesterol(TC) and triglyceride(TG) were tested. Results The body weight,weight gain, food intake and food utilization rate of rats in each dose group were normal. The results showed that the ALT in male rats decreased and there was a significant difference between the experimental group and the control group(P<0.01). Compared with the control group, the TC of male rats in the middle and high dose groups decreased significantly(P<0.01). Compared with the control group, the TC of female rats in the low and middle dose groups decreased significantly(P<0.05). Conclusion Dendrobium officinale has the effect of reducing ALT and TC in rats.
引文
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[2]吴建,王建方,方玲,等.国内铁皮石斛研究概况[J].中国药学杂志,2013,48(19):1610-1613.Wu J,Wang JF,Fang L,et al.Domestic research progress of Dendrobium officinale[J].Chin Pharm J,2013,48(19):1610-1613.
[3]李玲,邓晓兰,赵兴兵,等.铁皮石斛化学成分及药理作用研究进展[J].肿瘤医学,2011,1(2):90-94.Li L,Deng XL,Zhao XB,et al.Advances in studies on chemical constituents in Dendrobium candidum and their pharmacological effects[J].Anti-tumor Pharm,2011,1(2):90-94.
[4]吕圭源,颜美秋,成素红.铁皮石斛功效相关药理作用研究进展[J].中国中药杂志,2013,38(4):489-493.Lv GY,Yan MQ,Cheng SH,et al.Review of pharmacological activities of Dendrobium officinale based on traditional functions[J].China J Chin Mate Med,2013,38(4):489-493.
[5]吴昊姝,徐建华,陈立钻,等.铁皮石斛降血糖作用及其机制的研究[J].中国中药杂志,2004,29(2):160-163.Wu HS,Xu JH,Chen LZ,et al.Studies on anti-hyperglycemic effect and its mechanism of Dendrobium candidum[J].China J Chin Mater Med,2004,29(2):160-163.
[6]Charoenrungruang S,Chanvorachote P,Sritularak B,et al.Gigantol,a bibenzyl from Dendrobium draconis,inhibits the migratory behavior of nonsmall cell lung cancer cells[J].J Nat Prod,2014,77(6):1359-1366.
[7]Teixeira JA,Ng TB.The medicinal and pharmaceutical importance of Dendrobium species[J].Appl Microbiol Biotechnol,2017,101(6):2227-2239.
[8]Luo D,Qu C,Lin G,et al.Character and laxative activity of polysaccharides isolated from Dendrobium officinale[J].J Funct Food,2017,34:106-107.
[9]李媛媛,刘卓,欧阳盛荣,等.以转氨酶升高为首发表现的假肥大型肌营养不良临床分析[J].中华实用儿科临床杂志,2017,32(24):1877-1881.Li YY,Liu Z,Ouyang SR,et al.Clinical analysis of Duchenne Becker muscular dystrophy with transaminase elevation as the initial presentation[J].J Appl Clin Pediat,2017,32(24):1877-1881.
[10]吉英杰,何卫平.转氨酶升高的进行性肌营养不良61例[J].肝脏,2012,17(5):301-303.Ji YJ,He WP.Clinical analysis of 61 cases of progressive muscular dystrophy visiting as transaminase elevation[J].Chin Hepatol,2012,17(5):301-303.
[11]刘振,曾俊晟,曾胜,等.谷氨酰胺转氨酶与神经退行性疾病[J].中华医学遗传学杂志,2015,32(4):562-566.Liu Z,Zeng JM,Zeng S,et al.Transglutaminase and neurodegenerative diseases[J].Chin J Med Genet,2015,32(4):562-566.
[12]袁慧琦,梁楚燕,梁健,等.铁皮石斛对小鼠急性酒精性肝损伤的保护作用[J].暨南大学学报(自然科学与医学版),2015,37(5):385-387.Yuan HQ,Liang CY,Liang J,et al.Protection of Dendrobium officinale against acute alcoholic liver injury in mice[J].J Jinan Univ(Nat Sci Med Ed),2015,37(5):385-387.
[13]汤小华,陈素红,吕圭源,等.铁皮石斛对小鼠急性酒精性肝损伤模型SOD、MDA、GSH-Px的影响[J].浙江中医杂志,2010,45(5):369-370.Tang XH,Chen SH,Lv GY,et al.Effects of Dendrobium candidum on SOD,MDA and GSH-Px in mice model of acute alcoholic hepatic injury[J].Zhejiang J Tradit Chin Med,2010,45(5):369-370.
[14]张玲燕,柳庆君,王世佑,等.高血脂与胆石症的关系探讨[J].中国医药导报,2012,9(15):65-66.Zhang LY,Liu QJ,Wang SY,et al.Discussion on the relationship between hyperlipemia and cholecystolithiasis[J].China Med Her,2012,9(15):65-66.
[15]Kwon HM,Kim D,Hong BK,et al.Ultrastructural changes of the internal elastic lamina in experimental hypercholesterolemic porcine coronary arteries[J].J Korean Med Sci,1998,13(6):603-611.
[16]Perales S,Alejandre MJ,Palomino-Morales R,et al.Influence of cholesterol and fish oil dietary intake on nitric oxide-induced apoptosisin vascular smooth muscle cells[J].Nitric Oxide,2010,22(3):205-212.
[17]Vallejo-Vaz AJ,Kondapally SR,Cole D,et al.Familial hypercholesterolaemia:Aglobal call to arms[J].Atherosclerosis,2015,243(1):257-259.