枳椇子水提取液对急性酒精中毒家兔离体小肠运动的影响及其解酒机制探讨
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摘要
目的:探讨枳椇子水提取液对急性酒精中毒家兔离体小肠运动的影响及其解酒机制研究。方法:采用在体灌胃法和离体小肠灌流法复制两种家兔急性酒精中毒模型,观察枳椇子水提液对急性酒精中毒家兔离体小肠平滑肌收缩活动的影响以及该处的解酒机制。结果:急性在体灌胃酒精中毒家兔离体实验中,枳椇子水提取液高、中剂量组、解酒灵高剂量组对离体小肠平滑肌收缩均有抑制作用(P <0.05,P <0.01));急性离体酒精中毒的家兔离体实验中,枳椇子水提取液、解酒灵各剂量组对离体小肠平滑肌收缩均有抑制作用(P <0.05,P <0.01)。结论:枳椇子水提取液对两种模型的急性酒精中毒家兔离体小肠运动均有抑制作用,其机制可能是通过抑制小肠对乙醇的吸收,减缓乙醇氧化为乙醛的速度,使产生乙醛的量减少,是否与黄酮类化合物的二氢杨梅素有关,有待进一步研究。
Objective To investigate the effect of water extract of semen trifoliate on the movement of small intestine in rabbits with acute alcoholism and the mechanism of alcohol detoxication.Methods Two models of rabbits with acute alcoholism were replicated by the method of intragastric perfusion and isolated small intestine perfusion,and the effect of water extract on the contractile activity of isolated small intestinal smooth muscle of rabbits with acute alcoholism and the mechanism of alcohol removal were observed.Result In vitro experiment of acute alcoholic intoxication in rabbits:the high dose group and the middle dose group of the water extract of trifoliate and high dose detoxication of alcohol group had inhibitory effect on the contractile of isolated small intestine smooth muscle(P<0.05,P<0.01).In the isolated experiment of acute alcohol intoxication in rabbits:the group of the water extract of trifoliate and detoxication of alcohol in each dose was inhibited contraction of intestinal smooth muscle(P<0.05,P<0.01).Conclusion The water extract of trifoliate can inhibit the isolated small intestine movement of two models of acute alcoholism.The mechanism may be that inhibiting the absorption of ethanol to the small intestine,slowing down the oxidation of ethanol into acetaldehyde,and then reducing the amount of acetaldehyde.The question of whether it is related to the flavonoids of the two hydrogen myricetinm is to be studied further.
Objective To investigate the effect of water extract of semen trifoliate on the movement of small intestine in rabbits with acute alcoholism and the mechanism of alcohol detoxication.Methods Two models of rabbits with acute alcoholism were replicated by the method of intragastric perfusion and isolated small intestine perfusion,and the effect of water extract on the contractile activity of isolated small intestinal smooth muscle of rabbits with acute alcoholism and the mechanism of alcohol removal were observed.Result In vitro experiment of acute alcoholic intoxication in rabbits:the high dose group and the middle dose group of the water extract of trifoliate and high dose detoxication of alcohol group had inhibitory effect on the contractile of isolated small intestine smooth muscle(P<0.05,P<0.01).In the isolated experiment of acute alcohol intoxication in rabbits:the group of the water extract of trifoliate and detoxication of alcohol in each dose was inhibited contraction of intestinal smooth muscle(P<0.05,P<0.01).Conclusion The water extract of trifoliate can inhibit the isolated small intestine movement of two models of acute alcoholism.The mechanism may be that inhibiting the absorption of ethanol to the small intestine,slowing down the oxidation of ethanol into acetaldehyde,and then reducing the amount of acetaldehyde.The question of whether it is related to the flavonoids of the two hydrogen myricetinm is to be studied further.
引文
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[20]邓源,袁伯,胡晋红,等.解酒灵的药理实验研究[J].中草药,2000,31(6):446-448.
[2]余选良,朱肖鸿,冯舒婷.枳椇子治疗酒精性肝病现状[J].浙江中西医结合杂志,2017,27(4):342-344.
[3]王洁,周瑢,潘晓薇,等.葛根、葛花、枳椇子混合物对小鼠急性酒精中毒醒酒作用的研究[J].药学实践杂志,2017(5):98-401.
[4]陈绍红,柳海艳,陈丰,等.葛花与枳犋子在解酒毒时的配伍应用[C]//第十九届全国药学史本草学术研讨会暨2017年江苏省药学会药学史专业委员会年会论文集,江苏省药学会药学史专业委员会,2017:267-269.
[5]高万露,汪小海.急性酒精中毒药物治疗新进展[J].药学与临床研究,2015,23(1):59-61.
[6]柳海艳,钟赣生,李怡文.醇提和水提葛花枳椇子及其配伍对酒精性肝损伤大鼠肝脏抗氧化功能的影响[J].中华中医药杂志,2012,27(4):1181-1184.
[7]周红,王沁.急性酒精中毒对脑啡肽活性的影响及葛根素调控机理[J].中国中医急症,2008,17(6):817-818.
[8]胥学梅,吴锦丽,江文易,等.白花蛇舌草提取液对家兔不同离体肠段活动的效应研究[J].四川中医,2014,32(1):76-78.
[9]翟玉荣,刘波,莫镇涛,等.茉莉花叶水提物对小肠平滑肌的影响[J].中国老年学杂志,2015,35(11):2964-2965.
[10]曲红梅.急性酒精中毒内科急诊治疗疗效分析[J].中国现代药物应用,2018,12(3):113-114.
[11]Rong Li M.D.,Li Hu M.D.,Lingli Hu M.D.,e tal.E-valuation of Acute Alcohol Intoxication as the Primary Cause of Death:A Diagnostic Challenge for Forensic Pathologists[J].Journal of Forensic Sciences,2017,62(5):1203-1207.
[12]Gabriella Minera,Evan Robinson.Accidental Acute Alcohol Intoxication in Infants:Review and Case Report[J].Journal of Emergency Medicine,2014,47(5):834-838.
[13]冯娜.金银花葛花解酒功能的研究及功能性饮料的开发[D].山东师范大学,2013.
[14]胡义扬,方志红,崔剑巍,等.酒精性肝损伤与小肠通透性改变的关系及中药的干预作用[J].科学技术与工程,2005(22):80-81.
[15]卡力比夏提·艾木拉江,周玉东,闫书平,等.温度变化对家兔离体小肠平滑肌运动的影响[J].中国畜牧兽医,2016,43(8):2060-2065.
[16]王敏.魔芋粉对酒精首过代谢影响及对酒精性脑损伤保护作用的研究[D].西南大学,2017.
[17]王彦平,袁贵英,陈月英,等.枳椇子营养成分和总黄酮含量分析及评价[J].食品研究与开发,2016,37(24):21-24.
[18]侯小龙,王文清,施春阳,等.二氢杨梅素药理作用研究进展[J].中草药,2015,46(4):603-609.
[19]胡佳琦,郭岚,马丽梅.天然解酒药的研究[J].世界临床医学,2016,10(3):123-125.
[20]邓源,袁伯,胡晋红,等.解酒灵的药理实验研究[J].中草药,2000,31(6):446-448.