蒲公英抗肿瘤作用的研究进展
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摘要
中药蒲公英含有多种活性成分,包括多糖、黄酮、萜类、色素、植物甾醇、香豆素和有机酸等,这些活性成分具有重要的抗肿瘤作用,主要通过干预肿瘤细胞的信号转导、调控细胞周期和凋亡蛋白的表达,抑制肿瘤细胞的增殖和迁移等,从而有效遏制肿瘤的发展与恶化。本文就蒲公英中五大类活性成分的抗肿瘤作用和机制进行综述,旨在为蒲公英有效活性成分的抗肿瘤研究提供更多的参考依据。
As a traditional Chinese herb, taraxacum contains multiple active ingredients which play significant roles in anticancer action, such as polysaccharide, flavonoid, terpene, pigment, taraxasterol, coumarin, organic acid and so on. These active ingredients generally interfere in signal transduction of cancer cells, regulate cancer cell cycle and apoptotic protein expression, and inhibit proliferation and migration of cancer cells and so on, and thus effectively restrain tumor development and deterioration. This article summarized the anti-tumor mechanism of five active ingredients in taraxacum, aiming to provide more references for relevant research.
As a traditional Chinese herb, taraxacum contains multiple active ingredients which play significant roles in anticancer action, such as polysaccharide, flavonoid, terpene, pigment, taraxasterol, coumarin, organic acid and so on. These active ingredients generally interfere in signal transduction of cancer cells, regulate cancer cell cycle and apoptotic protein expression, and inhibit proliferation and migration of cancer cells and so on, and thus effectively restrain tumor development and deterioration. This article summarized the anti-tumor mechanism of five active ingredients in taraxacum, aiming to provide more references for relevant research.
引文
[1]张建.蒲公英属植物繁殖生物学研究[D].沈阳农业大学,2013.
[2]国家药典委员会.中华人民共和国药典[S].一部.北京:中国医药科技出版社, 2010:331.
[3]谢沈阳,杨晓源,丁章贵,等.蒲公英的化学成份及其药理作用[J].天然产物研究与开发, 2012, 24(S1):141-151.
[4]屠国昌.蒲公英化学成分、药理作用和临床应用[J].海峡药学, 2012, 24(5):33-35. doi:10.3969/j. issn.1006-3765.2012.05.016.
[5]江苏新医学院编.中药大辞典附编[M].上海人民出版社, 1979.
[6]肖玫,杨进,曹玉华.蒲公英的营养价值及其开发利用[J].中国食物与营养, 2005(4):47-48. doi:10.3969/j.issn.1006-9577.2005.04.015.
[7]吴晓春,杜胜利,陈海生.蒲公英的研究与应用[J].药学实践杂志, 2002(4):246-248. doi:10.3969/j. issn.1006-0111.2002.04.026.
[8]唐美霞,王福宁,王涛.蒲公英利用现状及开发前景的探讨[J].青海草业, 2015, 24(1):39-44, 47. doi:10.3969/j.issn.1008-1445.2015.01.009.
[9]Li XH, He XR, Zhou YY, et al. Taraxacum mongolicum extract induced endoplasmic reticulum stress associatedapoptosis in triple-negative breast cancer cells[J]. J Ethnopharmacol, 2017, 206:55-64. doi:10.1016/j.jep.2017.04.025.
[10]Koo HN, Hong SH, Song BK, et al. Taraxacum officinale induces cytotoxicity through TNF-alpha and IL-1alpha secretion in Hep G2 cells[J]. Life Sci, 2004, 74(9):1149-1157. doi:10.1016/j. lfs.2003.07.030.
[11]沈敬华,杨丽敏,张林娜,等.五种中药提取物抗肿瘤作用的研究[J].内蒙古医学院学报, 2005(4):46-48. doi:10.3969/j. issn.1004-2113.2005.04.010.
[12]陈红林,乔华,孙体健.蒲公英花提取物的体外抗肿瘤活性研究[J].中国药物与临床, 2014, 14(9):1179-1181.doi:10.11655/zgywylc2014.09.007.
[13]Rehman G, Hamayun M, Iqbal A, et al. Effect of Methanolic Extract of Dandelion Roots on Cancer Cell Lines and AMPActivated Protein Kinase Pathway[J]. Front Pharmacol,2017, 8:875. doi:10.3389/fphar.2017.00875.
[14]Ovadje P, Chochkeh M, Akbari-Asl P, et al. Selective induction of apoptosis and autophagy through treatment with dandelion root extract in human pancreatic cancer cells[J]. Pancreas, 2012, 41(7):1039-1047. doi:10.1097/MPA.0b013e31824b22a2.
[15]孙继梅,郑伟,周秀珍,等.蒲公英体外抑菌活性的研究[J].中国误诊学杂志, 2009, 9(11):2542-2543.
[16]王春花,齐洪军.中药抗幽门螺旋杆菌研究进展[J].陕西中医, 2011, 32(6):763-765,封3. doi:10.3969/j. issn.1000-7369.2011.06.078.
[17]尤春来,韩兆丰,朱丹,等.蒲公英对大鼠胃酸分泌的抑制作用及其时胃酸刺激药的影响[J].中药药理与临床,1994(2):23-26.
[18]崔闽鲁,黄鼎明.蒲公英合剂治疗胃溃疡、浅表性胃炎45例疗效观察[J].福建中医药, 1992(3):17.
[19]王秋亚.蒲公英有效成分的提取及应用研究进展[J].江苏农业科学, 2016, 44(8):21-24. doi:10.15889/j. issn.1002-1302.2016.08.005.
[20]李雪石,张彦文.蒲公英水提取物对链脲佐菌素致糖尿病大鼠的降血糖作用及其机制[J].中草药, 2013, 44(7):863-868. doi:10.7501/j. issn.0253-2670.
[21]俞红,李锦兰.蒲公英对小鼠免疫功能的影响[J].贵阳医学院学报, 1997(2):37-39.
[22]陈福星,陈文英,钟秀会.蒲公英多糖对小鼠免疫机能的影响[J].中国兽医杂志, 2009, 45(3):19-20. doi:10.3969/j. issn.0529-6005.2009.03.008.
[23]于新慧,石学魁,张晓莉,等.蒲公英对小鼠免疫功能的调节研究[J].牡丹江医学院学报, 2008, 29(4):11-13.doi:10.3969/j. issn.1001-7550.2008.04.006.
[24]张智,刘洋,尹文哲,等.蒲公英总黄酮复合酶酶法提取工艺及抗氧化活性研究[J].食品与机械, 2018, 34(2):143-148. doi:CNKI:SUN:SPJX.0.2018-02-033.
[25]黄丹,顾英.蒲公英黄酮与多糖对DPPH清除能力比较研究[J].饲料研究, 2015,(23):59-62.
[26]葛明明,缪月英,孙丽娜,等.蒲公英根多糖的抗氧化活性研究[J].黑龙江医药科学, 2014, 37(2):39-41. doi:10.3969/j. issn.1008-0104.2014.02.020.
[27]Chatterjee SJ, Ovadje P, Mousa M, et al. The efficacy of dandelion root extract in inducing apoptosis in drug-resistant human melanoma cells[J]. Evid Based Complement Alternat Med, 2011, 2011:129045. doi:10.1155/2011/129045.
[28]牛虎.蒲公英多糖对乳腺癌细胞增殖和凋亡的影响[D].山东大学, 2017.
[29]Ovadje P, Hamm C, Pandey S. Efficient induction of extrinsic cell death by dandelion root extract in human chronic myelomonocytic leukemia(CMML)cells[J]. PLoS One,2012, 7(2):e30604. doi:10.1371/journal. pone.0030604.
[30]孙新城,李丹,罗宇,等.蒲公英内生真菌PG23抗癌活性的初步研究[J].动物医学进展, 2012, 33(9):78-81. doi:10.3969/j. issn.1007-5038.2012.09.018.
[31]Tan B, Shi HL, Ji G, et al.[Effects of taraxerol and taraxerol acetate on cell cycle and apoptosis of human gastric epithelial cell line AGS][J]. Zhong Xi Yi Jie He Xue Bao, 2011,9(6):638-642.
[32]蒙旭.乙酰蒲公英萜醇影响Hep G2细胞增殖、凋亡及迁移的实验研究[D].昆明医科大学, 2016.
[33]史栋栋.蒲公英代谢物的抗乳腺癌细胞MCF-7活性成分及其作用机理研究[D].上海交通大学, 2014.
[34]周玲玉.β-谷甾醇对人肺癌细胞增殖、凋亡影响的初步研究[D].重庆医科大学, 2016. doi:CNKI:CDMD:2.1016.783452.
[35]宋冬雪,汲晨锋,季宇彬.植物甾醇抗肿瘤作用研究进展[J].牡丹江医学院学报, 2011, 32(0):79-81. doi:10.3969/j. issn.1001-7550.2011.04.043.
[36]陶仕英,牛建昭,王继峰,等.β-谷甾醇对T47D细胞增殖和细胞周期的影响及作用机制探讨[J].世界科学技术-中医药现代化, 2015, 17(2):362-366. doi:10.11842/wst.2015.02.012.
[37]Baskar AA, Ignacimuthu S, Paulraj GM, et al. Chemopreventive potential of beta-Sitosterol in experimental colon cancer model--an in vitro and In vivo study[J]. BMC Complement Altern Med, 2010, 10:24. doi:10.1186/1472-6882-10-24.
[38]朱文振,马龙,李国荣.黄酮类化合物的抗癌作用及作用机制[J].生命科学, 2012, 24(5):444-449.
[39]黄丹.蒲公英黄酮及多糖主要生物活性的比较研究[D].锦州医科大学, 2016. doi:CNKI:CDMD:2.1016.215206.
[40]王晓,吴龙火,黄晓平,等.黄酮类化合物的抗癌机制[J].中国民族民间医药, 2009, 18(22):1-3. doi:10.3969/j.issn.1007-8517.2009.22.001.
[41]施树云,张宇平,周宏灏,等.管花蒲公英中抗肿瘤活性化合物的快速筛选研究[J].药物分析杂志, 2011, 31(3):471-475.
[42]李喜凤,郝哲,邱天宝,等.蒲公英中有机酸类成分的提取工艺研究[J].中成药, 2011, 33(2):262-265. doi:10.3969/j. issn.1001-1528.2011.02.018.
[43]Ho HH, Chang CS, Ho WC, et al. Anti-metastasis effects of gallic acid on gastric cancer cells involves inhibition of NF-kappa B activity and downregulation of PI3K/AKT/small GTPase signals[J]. Food Chem Toxicol, 2010, 48(8-9):2508-2516. doi:10.1016/j. fct.2010.06.024.
[44]姚素艳,李全胜,郑德宇.绿原酸对体外培养的人鼻咽癌细胞株CNE-1的作用[J].西安交通大学学报(医学版),2014, 35(6):837-842. doi:10.7652/jdyxb201406022.
[45]Yoon JY, Cho HS, Lee JJ, et al. Novel TRAIL sensitizer Taraxacum officinale F. H. Wigg enhances TRAIL-induced apoptosis in Huh7 cells[J]. Mol Carcinog, 2016, 55(4):387-96. doi:10.1002/mc.22288.
[2]国家药典委员会.中华人民共和国药典[S].一部.北京:中国医药科技出版社, 2010:331.
[3]谢沈阳,杨晓源,丁章贵,等.蒲公英的化学成份及其药理作用[J].天然产物研究与开发, 2012, 24(S1):141-151.
[4]屠国昌.蒲公英化学成分、药理作用和临床应用[J].海峡药学, 2012, 24(5):33-35. doi:10.3969/j. issn.1006-3765.2012.05.016.
[5]江苏新医学院编.中药大辞典附编[M].上海人民出版社, 1979.
[6]肖玫,杨进,曹玉华.蒲公英的营养价值及其开发利用[J].中国食物与营养, 2005(4):47-48. doi:10.3969/j.issn.1006-9577.2005.04.015.
[7]吴晓春,杜胜利,陈海生.蒲公英的研究与应用[J].药学实践杂志, 2002(4):246-248. doi:10.3969/j. issn.1006-0111.2002.04.026.
[8]唐美霞,王福宁,王涛.蒲公英利用现状及开发前景的探讨[J].青海草业, 2015, 24(1):39-44, 47. doi:10.3969/j.issn.1008-1445.2015.01.009.
[9]Li XH, He XR, Zhou YY, et al. Taraxacum mongolicum extract induced endoplasmic reticulum stress associatedapoptosis in triple-negative breast cancer cells[J]. J Ethnopharmacol, 2017, 206:55-64. doi:10.1016/j.jep.2017.04.025.
[10]Koo HN, Hong SH, Song BK, et al. Taraxacum officinale induces cytotoxicity through TNF-alpha and IL-1alpha secretion in Hep G2 cells[J]. Life Sci, 2004, 74(9):1149-1157. doi:10.1016/j. lfs.2003.07.030.
[11]沈敬华,杨丽敏,张林娜,等.五种中药提取物抗肿瘤作用的研究[J].内蒙古医学院学报, 2005(4):46-48. doi:10.3969/j. issn.1004-2113.2005.04.010.
[12]陈红林,乔华,孙体健.蒲公英花提取物的体外抗肿瘤活性研究[J].中国药物与临床, 2014, 14(9):1179-1181.doi:10.11655/zgywylc2014.09.007.
[13]Rehman G, Hamayun M, Iqbal A, et al. Effect of Methanolic Extract of Dandelion Roots on Cancer Cell Lines and AMPActivated Protein Kinase Pathway[J]. Front Pharmacol,2017, 8:875. doi:10.3389/fphar.2017.00875.
[14]Ovadje P, Chochkeh M, Akbari-Asl P, et al. Selective induction of apoptosis and autophagy through treatment with dandelion root extract in human pancreatic cancer cells[J]. Pancreas, 2012, 41(7):1039-1047. doi:10.1097/MPA.0b013e31824b22a2.
[15]孙继梅,郑伟,周秀珍,等.蒲公英体外抑菌活性的研究[J].中国误诊学杂志, 2009, 9(11):2542-2543.
[16]王春花,齐洪军.中药抗幽门螺旋杆菌研究进展[J].陕西中医, 2011, 32(6):763-765,封3. doi:10.3969/j. issn.1000-7369.2011.06.078.
[17]尤春来,韩兆丰,朱丹,等.蒲公英对大鼠胃酸分泌的抑制作用及其时胃酸刺激药的影响[J].中药药理与临床,1994(2):23-26.
[18]崔闽鲁,黄鼎明.蒲公英合剂治疗胃溃疡、浅表性胃炎45例疗效观察[J].福建中医药, 1992(3):17.
[19]王秋亚.蒲公英有效成分的提取及应用研究进展[J].江苏农业科学, 2016, 44(8):21-24. doi:10.15889/j. issn.1002-1302.2016.08.005.
[20]李雪石,张彦文.蒲公英水提取物对链脲佐菌素致糖尿病大鼠的降血糖作用及其机制[J].中草药, 2013, 44(7):863-868. doi:10.7501/j. issn.0253-2670.
[21]俞红,李锦兰.蒲公英对小鼠免疫功能的影响[J].贵阳医学院学报, 1997(2):37-39.
[22]陈福星,陈文英,钟秀会.蒲公英多糖对小鼠免疫机能的影响[J].中国兽医杂志, 2009, 45(3):19-20. doi:10.3969/j. issn.0529-6005.2009.03.008.
[23]于新慧,石学魁,张晓莉,等.蒲公英对小鼠免疫功能的调节研究[J].牡丹江医学院学报, 2008, 29(4):11-13.doi:10.3969/j. issn.1001-7550.2008.04.006.
[24]张智,刘洋,尹文哲,等.蒲公英总黄酮复合酶酶法提取工艺及抗氧化活性研究[J].食品与机械, 2018, 34(2):143-148. doi:CNKI:SUN:SPJX.0.2018-02-033.
[25]黄丹,顾英.蒲公英黄酮与多糖对DPPH清除能力比较研究[J].饲料研究, 2015,(23):59-62.
[26]葛明明,缪月英,孙丽娜,等.蒲公英根多糖的抗氧化活性研究[J].黑龙江医药科学, 2014, 37(2):39-41. doi:10.3969/j. issn.1008-0104.2014.02.020.
[27]Chatterjee SJ, Ovadje P, Mousa M, et al. The efficacy of dandelion root extract in inducing apoptosis in drug-resistant human melanoma cells[J]. Evid Based Complement Alternat Med, 2011, 2011:129045. doi:10.1155/2011/129045.
[28]牛虎.蒲公英多糖对乳腺癌细胞增殖和凋亡的影响[D].山东大学, 2017.
[29]Ovadje P, Hamm C, Pandey S. Efficient induction of extrinsic cell death by dandelion root extract in human chronic myelomonocytic leukemia(CMML)cells[J]. PLoS One,2012, 7(2):e30604. doi:10.1371/journal. pone.0030604.
[30]孙新城,李丹,罗宇,等.蒲公英内生真菌PG23抗癌活性的初步研究[J].动物医学进展, 2012, 33(9):78-81. doi:10.3969/j. issn.1007-5038.2012.09.018.
[31]Tan B, Shi HL, Ji G, et al.[Effects of taraxerol and taraxerol acetate on cell cycle and apoptosis of human gastric epithelial cell line AGS][J]. Zhong Xi Yi Jie He Xue Bao, 2011,9(6):638-642.
[32]蒙旭.乙酰蒲公英萜醇影响Hep G2细胞增殖、凋亡及迁移的实验研究[D].昆明医科大学, 2016.
[33]史栋栋.蒲公英代谢物的抗乳腺癌细胞MCF-7活性成分及其作用机理研究[D].上海交通大学, 2014.
[34]周玲玉.β-谷甾醇对人肺癌细胞增殖、凋亡影响的初步研究[D].重庆医科大学, 2016. doi:CNKI:CDMD:2.1016.783452.
[35]宋冬雪,汲晨锋,季宇彬.植物甾醇抗肿瘤作用研究进展[J].牡丹江医学院学报, 2011, 32(0):79-81. doi:10.3969/j. issn.1001-7550.2011.04.043.
[36]陶仕英,牛建昭,王继峰,等.β-谷甾醇对T47D细胞增殖和细胞周期的影响及作用机制探讨[J].世界科学技术-中医药现代化, 2015, 17(2):362-366. doi:10.11842/wst.2015.02.012.
[37]Baskar AA, Ignacimuthu S, Paulraj GM, et al. Chemopreventive potential of beta-Sitosterol in experimental colon cancer model--an in vitro and In vivo study[J]. BMC Complement Altern Med, 2010, 10:24. doi:10.1186/1472-6882-10-24.
[38]朱文振,马龙,李国荣.黄酮类化合物的抗癌作用及作用机制[J].生命科学, 2012, 24(5):444-449.
[39]黄丹.蒲公英黄酮及多糖主要生物活性的比较研究[D].锦州医科大学, 2016. doi:CNKI:CDMD:2.1016.215206.
[40]王晓,吴龙火,黄晓平,等.黄酮类化合物的抗癌机制[J].中国民族民间医药, 2009, 18(22):1-3. doi:10.3969/j.issn.1007-8517.2009.22.001.
[41]施树云,张宇平,周宏灏,等.管花蒲公英中抗肿瘤活性化合物的快速筛选研究[J].药物分析杂志, 2011, 31(3):471-475.
[42]李喜凤,郝哲,邱天宝,等.蒲公英中有机酸类成分的提取工艺研究[J].中成药, 2011, 33(2):262-265. doi:10.3969/j. issn.1001-1528.2011.02.018.
[43]Ho HH, Chang CS, Ho WC, et al. Anti-metastasis effects of gallic acid on gastric cancer cells involves inhibition of NF-kappa B activity and downregulation of PI3K/AKT/small GTPase signals[J]. Food Chem Toxicol, 2010, 48(8-9):2508-2516. doi:10.1016/j. fct.2010.06.024.
[44]姚素艳,李全胜,郑德宇.绿原酸对体外培养的人鼻咽癌细胞株CNE-1的作用[J].西安交通大学学报(医学版),2014, 35(6):837-842. doi:10.7652/jdyxb201406022.
[45]Yoon JY, Cho HS, Lee JJ, et al. Novel TRAIL sensitizer Taraxacum officinale F. H. Wigg enhances TRAIL-induced apoptosis in Huh7 cells[J]. Mol Carcinog, 2016, 55(4):387-96. doi:10.1002/mc.22288.