扎布耶盐山羊大肠杆菌分离鉴定及药敏试验
|
摘要
为掌握西藏自治区仲巴县帕江乡扎布耶盐山羊的发病情况,采集发病羊的粪便、血液、肝脏、脾脏、胸腔、腹腔和心包腔内蓄积的浆液性纤维性渗出物等进行病原菌分离鉴定及药敏试验。经病原菌分离鉴定,该地区扎布耶盐山羊羔羊感染的病原菌为大肠杆菌,检出率为97.14%(34/35);药敏试验结果表明,该研究分离得到的大肠杆菌对头孢唑林、青霉素、阿莫西林、头孢他啶、氟苯尼考、头孢拉啶6种药物高度敏感。该调查研究为仲巴县帕江乡扎布耶盐山羊大肠杆菌病的防治提供了诊断方法和用药方案。
To determine the causative agents of the bacterial diseases of Zabuye salt goats in Pajiang Township of Zhongba County of Tibet, the clinical samples of fecal, blood, liver, spleen as well as the serous and fibrous exudates in chest cavity, abdominal cavity, pericardial cavity were recovered from the diseased goats to perform pathogenic agents isolation, identification and antimicrobial sensitivity testing. The results demonstrated that 97.14%(34/35) of the recovered samples were positive for the presence of Escherichia coli, indicating that Escherichia coli was the dominant pathogen associated with the infectious disease of the Zabuye salt goat lambs in this area; the isolates were highly sensitive to cefazolin, penicillin, amoxicillin, ceftazidime,florfenicol and cefradine. This study provides a diagnostic method and a medication plan for colibacillosis prevalent in Zabuye salt goat lambs in this region.
To determine the causative agents of the bacterial diseases of Zabuye salt goats in Pajiang Township of Zhongba County of Tibet, the clinical samples of fecal, blood, liver, spleen as well as the serous and fibrous exudates in chest cavity, abdominal cavity, pericardial cavity were recovered from the diseased goats to perform pathogenic agents isolation, identification and antimicrobial sensitivity testing. The results demonstrated that 97.14%(34/35) of the recovered samples were positive for the presence of Escherichia coli, indicating that Escherichia coli was the dominant pathogen associated with the infectious disease of the Zabuye salt goat lambs in this area; the isolates were highly sensitive to cefazolin, penicillin, amoxicillin, ceftazidime,florfenicol and cefradine. This study provides a diagnostic method and a medication plan for colibacillosis prevalent in Zabuye salt goat lambs in this region.
引文
[1]祝艳华,陈颢,张国斌,等.几种不同措施对高寒牧区羔羊大肠杆菌病的防治效果研究[J].中国草食动物科学,2014,34(4):55-57.
[2]陈颢,张国斌,王治仓,等.高寒地区羔羊大肠杆菌病综合防治[J].畜牧兽医杂志,2013,32(6):120-121.
[3]百度经验.羊大肠杆菌病[EB/OL].(2017-09-25).https://jingyan.baidu.com/article/e73e26c0c2831a24adb6a7b2.html.
[4]李强.羔羊大肠杆菌病的临床诊治[J].畜牧与饲料科学,2012,33(9):90.
[5]韩有库,胡希荣,孙长金,等.羔羊大肠杆菌病防治方法的研究[J].吉林农业大学学报,1980(1):49-55.
[6]百度百科.仲巴县[EB/OL].(2018-09-08).https://baike.baidu.com/item/%E4%BB%B2%E5%B7%B4%E5%8E%BF/8613836?fr=aladdin.
[7]张忠,陈颢,王治仓,等.高寒牧区绵羊大肠杆菌病发病规律及病因调查研究[J].家畜生态学报,2014,35(2):67-69.
[8]东主措.一例羔羊大肠杆菌病的诊断与药敏试验[J].中兽医学杂志,2015(8):4.
[9]陈杖榴.兽医药理学[M].3版.北京:中国农业出版社,2009.
[10]陈溥言.兽医传染病学[M].6版.北京:中国农业出版社,2013:108-117.
[11]全球品牌畜牧网.羔羊大肠杆菌病[EB/OL].(2015-04-13).http://www.ppxmw.com/zt186/.
[12]陆承平.兽医微生物学[M].5版.北京:中国农业出版社,2013.
[13]马利青,陆艳,裴全邦,等.羔羊致病性大肠杆菌病的分离培养及防治[J].青海畜牧兽医杂志,2017,47(4):6-10.
[2]陈颢,张国斌,王治仓,等.高寒地区羔羊大肠杆菌病综合防治[J].畜牧兽医杂志,2013,32(6):120-121.
[3]百度经验.羊大肠杆菌病[EB/OL].(2017-09-25).https://jingyan.baidu.com/article/e73e26c0c2831a24adb6a7b2.html.
[4]李强.羔羊大肠杆菌病的临床诊治[J].畜牧与饲料科学,2012,33(9):90.
[5]韩有库,胡希荣,孙长金,等.羔羊大肠杆菌病防治方法的研究[J].吉林农业大学学报,1980(1):49-55.
[6]百度百科.仲巴县[EB/OL].(2018-09-08).https://baike.baidu.com/item/%E4%BB%B2%E5%B7%B4%E5%8E%BF/8613836?fr=aladdin.
[7]张忠,陈颢,王治仓,等.高寒牧区绵羊大肠杆菌病发病规律及病因调查研究[J].家畜生态学报,2014,35(2):67-69.
[8]东主措.一例羔羊大肠杆菌病的诊断与药敏试验[J].中兽医学杂志,2015(8):4.
[9]陈杖榴.兽医药理学[M].3版.北京:中国农业出版社,2009.
[10]陈溥言.兽医传染病学[M].6版.北京:中国农业出版社,2013:108-117.
[11]全球品牌畜牧网.羔羊大肠杆菌病[EB/OL].(2015-04-13).http://www.ppxmw.com/zt186/.
[12]陆承平.兽医微生物学[M].5版.北京:中国农业出版社,2013.
[13]马利青,陆艳,裴全邦,等.羔羊致病性大肠杆菌病的分离培养及防治[J].青海畜牧兽医杂志,2017,47(4):6-10.