类风湿关节炎患者血清sCD163测定的临床价值
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摘要
目的探讨类风湿关节炎(rheumatoid arthritis,RA)患者血清s CD163检测的临床价值。方法采用ELISA法检测了2016年1月至2017年10月在上海市控江医院就诊的88名RA患者和85名健康个体的血清s CD163含量,分析其与患者血清C反应蛋白(CRP)、血沉(ESR)、中性粒细胞淋巴细胞比值(NLR)和DAS-28评分之间的关系。结果 RA患者血清s CD163含量[(798±245) ng/ml]较健康个体[(426±240) ng/ml]明显增高,且与患者的CRP、ESR、NLR和DAS-28均呈正相关。结论 RA患者的单核巨噬细胞系统处于过度活化状态。s CD163可以用于评价RA患者的疾病活动度。
Objective To investigate the clinical significance of serum s CD163 detection in patients with rheumatoid arthritis( RA).Methods Serum s CD163 concentrations in 88 RA patients and 85 healthy controls were detected with ELISA,and the relationship between s CD163 and C-reactive protein( CRP),erythrocyte sedimentation rate( ESR),neutrophil to lymphocyte ratio( NLR),DAS-28 scores were analyzed accordingly.Results Serum s CD163 level in RA patients [( 798±245) ng/ml]was significantly increased,as compared with that of the healthy controls [( 426±240) ng/ml],ant it was positively correlated with the patient CRP,ESR,NLR and DAS-28.Conclusion Monocyte-macrophage was over-activated in RA patients. s CD163 could be used as a useful tool for disease activity assessment in RA patients.
Objective To investigate the clinical significance of serum s CD163 detection in patients with rheumatoid arthritis( RA).Methods Serum s CD163 concentrations in 88 RA patients and 85 healthy controls were detected with ELISA,and the relationship between s CD163 and C-reactive protein( CRP),erythrocyte sedimentation rate( ESR),neutrophil to lymphocyte ratio( NLR),DAS-28 scores were analyzed accordingly.Results Serum s CD163 level in RA patients [( 798±245) ng/ml]was significantly increased,as compared with that of the healthy controls [( 426±240) ng/ml],ant it was positively correlated with the patient CRP,ESR,NLR and DAS-28.Conclusion Monocyte-macrophage was over-activated in RA patients. s CD163 could be used as a useful tool for disease activity assessment in RA patients.
引文
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[9]马剑达,郑东辉,朱浪静,等.病情活动性评分在类风湿关节炎患者治疗中的应用[J].中华关节外科杂志(电子版),2014,8(1):109-112.DOI:10.3877/cma.j.issn.1674-134x.2014.01.021.
[10] Li Y,Lee PY,Reeves WH.Monocyte and macrophage abnormalities in systemic lupus erythematosus[J]. Arch Immunol Ther Exp(Warsz),2010,58(5):355-364.DOI:10.1007/s0005-010-0093-y.
[11] Mao TK,Lian ZX,Selmi C,et al.Altered monocyte responses to defined TLR ligands in patients with primary biliary cirrhosis[J].Hepatology,2005,42(4):802-808.DOI:10.1002/hep.20859.
[12] Slama IB,Allali F,Lakhdar T,et al. Reliability and validity of CDAI and SDAI indices in comparison to DAS-28 index in Moroccan patients with rheumatoid arthritis[J]. BMC Musculoskelet Disord,2015,16:268.DOI:10.1186/s12891-015-0718-8.
[13] Weaver LK,Pioli PA,Wardwell K,et al.Up-regulation of human monocyte CD163 upon activation of cell-surface Toll-like receptors[J].J Leukoc Biol,2007,81(3):663-671.DOI:1189/116.0706428.
[2] Zhi Y,Gao P,Xin X,et al.Clinical significance of s CD163 and its possible role in asthma(Review)[J]. Mol Med Rep,2017,15(5):2931-2939.DOI:10.3892/mmr.2017.6393.
[3] Smolen Js,Aletaha D,Barton A,et al.Rheumatoid arthritis[J]. Nat Rev Dis Primers,2018,4:18001.DOI:10.1038/nrdp.2018.1.
[4] Mcinnes IB,Schett G.The pathogenesis of rheumatoid arthritis[J].N Engl J Med,2011,365(23):2205-2219.DOI:10.1056/NEJMra1004965.
[5] Nicholson LB,Raveney BJ,Munder M.Monocyte dependent regulation of autoimmune inflammation[J]. Curr Mol Med,2009,9(1):23-29.DOI:10.2174/156652409787314499.
[6] Peng QL,Zhang YL,Shu XM,et al.Elevated serum levels of soluble CD163 in polymyositis and dermatomyositis:associated with macrophage infiltration in muscle tissue[J]. J Rheumatol,2015,42(6):979-987.DOI:10.3899/jrheum.141307.
[7] Nakayama W,Jinnin M,Makino K,et al.CD163 expression is increased in the involved skin and sera of patients with systemic lupus erythematosus[J]. Eur J Dermatol,2012,22(4):512-517.DOI:10.1684/ejd.2012.1756.
[8] Gronbaek H,Kreutzfeldt M,Kazankov K,et al.Single-centre experience of the macrophage activation marker soluble(s)CD163-associations with disease activity and treatment response in patients with autoimmune hepatitis[J]. Aliment Pharmacol Ther,2016,44(10):1062-1070.DOI:10.1111/apt.13801.
[9]马剑达,郑东辉,朱浪静,等.病情活动性评分在类风湿关节炎患者治疗中的应用[J].中华关节外科杂志(电子版),2014,8(1):109-112.DOI:10.3877/cma.j.issn.1674-134x.2014.01.021.
[10] Li Y,Lee PY,Reeves WH.Monocyte and macrophage abnormalities in systemic lupus erythematosus[J]. Arch Immunol Ther Exp(Warsz),2010,58(5):355-364.DOI:10.1007/s0005-010-0093-y.
[11] Mao TK,Lian ZX,Selmi C,et al.Altered monocyte responses to defined TLR ligands in patients with primary biliary cirrhosis[J].Hepatology,2005,42(4):802-808.DOI:10.1002/hep.20859.
[12] Slama IB,Allali F,Lakhdar T,et al. Reliability and validity of CDAI and SDAI indices in comparison to DAS-28 index in Moroccan patients with rheumatoid arthritis[J]. BMC Musculoskelet Disord,2015,16:268.DOI:10.1186/s12891-015-0718-8.
[13] Weaver LK,Pioli PA,Wardwell K,et al.Up-regulation of human monocyte CD163 upon activation of cell-surface Toll-like receptors[J].J Leukoc Biol,2007,81(3):663-671.DOI:1189/116.0706428.