马占相思树干叶片与木屑对紫芝生长的影响
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摘要
为提高相思树加工副产品的利用率,同时拓宽紫芝栽培原料,开展了马占相思树加工中产生的干叶片与木屑对紫芝生长影响试验。结果表明:培养基中添加马占相思干叶片粉碎物对紫芝生长具有一定的促进作用。PDA母种培养基中添加相思干叶片100~150 g/L,可提高菌丝生长速度至79~85 mm/d;原种、栽培种培养基中分别添加150~250、200~300 g/kg的马占相思叶片,菌丝生长速度分别达到65、94 mm/d,明显高于对照;菌丝长满菌瓶的时间分别比对照缩短5~6 d;马占相思木屑与棉籽壳以45∶48(w/w)比例配比的出菇培养基中添加200~250 g/kg干叶片,可使生物学转化率达69%~73%。
In order to increase the utilization rate of by-products of processed Acacia mangium,and broaden the raw materials for cultivation of Ganoderma sinense,we conducted experiments to study the effects of dried leaf and sawdust from A.mangium on the growth of G.sinense.The results showed that adding the smashed A.mangium dried leaves into culture medium had a certain promoting effect on the growth of G.sinense.Adding 100 ~ 150 g/L A.mangium dried leaves into PDA medium for mother strain could increase the mycelial growth rate of G.sinense to 79 ~ 85 mm/d.Adding 150 ~ 250 g/kg(or 200 ~ 300 g/kg) A.mangium dried leaves into PDA medium for protospecies(or cultispecies) could increase the mycelial growth rate to 65 mm/d(or 94 mm/d),which was obviously higher than that of the control.The mycelial overgrowth time of G.sinense in the PDA medium added by A.mangium dried leaves was 5 ~ 6 days shorter than that of the control.Adding 200 ~ 250 g/kg A.mangium dried leaves into the fruiting medium containing A.mangium sawdust and cottonseed hull(45 ∶ 48,w/w) could enhance the biological conversion rate to 69% ~ 73%.
In order to increase the utilization rate of by-products of processed Acacia mangium,and broaden the raw materials for cultivation of Ganoderma sinense,we conducted experiments to study the effects of dried leaf and sawdust from A.mangium on the growth of G.sinense.The results showed that adding the smashed A.mangium dried leaves into culture medium had a certain promoting effect on the growth of G.sinense.Adding 100 ~ 150 g/L A.mangium dried leaves into PDA medium for mother strain could increase the mycelial growth rate of G.sinense to 79 ~ 85 mm/d.Adding 150 ~ 250 g/kg(or 200 ~ 300 g/kg) A.mangium dried leaves into PDA medium for protospecies(or cultispecies) could increase the mycelial growth rate to 65 mm/d(or 94 mm/d),which was obviously higher than that of the control.The mycelial overgrowth time of G.sinense in the PDA medium added by A.mangium dried leaves was 5 ~ 6 days shorter than that of the control.Adding 200 ~ 250 g/kg A.mangium dried leaves into the fruiting medium containing A.mangium sawdust and cottonseed hull(45 ∶ 48,w/w) could enhance the biological conversion rate to 69% ~ 73%.
引文
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[2]张卫国,刘欣,陈永泉.灵芝多糖的研究进展[J].饮料工业,2003,6(4):5-9.
[3]包海鹰,王欣宇.紫芝的化学成分研究进展[J].菌物研究,2014,12(4):187-196.
[4]钟礼义.桉树栽培紫芝及营养成分分析[J].中国食用菌,2014,33(3):24-26.
[5]李喜范,王培进,薛峰,等.东北地区紫芝短段木栽培技术[J].食用菌,2012(4):33-35.
[6]吴晓明,方树平.仿野生灵芝栽培技术[J].浙江食用菌,2008,16(3):42-43.
[7]金鑫,刘宗敏,黄羽佳,等.我国灵芝栽培现状及发展趋势[J].食药用菌,2016,24(1):33-37.
[8]郭翠英,陈方顺,黄维南,等.速生丰产的食用菌用材——大叶相思[J].中国食用菌,1998(4):15-16.
[9]张胜,刘子雷,陈薇,等.马占相思树皮废弃物酸水解及活性炭制备研究[J].中南林业科技大学学报,2017,37(4):100-108.
[10]Maharani Rizki,Yutaka Tamai.Effects of different nitrogen rich substrates and their combination to the yield performance of oyster mushroom(Pleurotus ostreatus)[J].World J Microbiol Biotechnol,2011,27(11):1695-1720.
[11]林芳能,董晓娜,陈喜蓉,等.竹荪栽培基质筛选研究[J].热带林业,2012,40(1):45-47.
[12]谢左章.马占相思叶粉饲用开发价值与经济预测[J].广东林业科技,1998,14(3):23-26.
[13]许松葵,薛立,杨鹏,等.马占相思.5个树种叶中养分含量养分利用效率研究[J].广东林业科技,2003,19(2):13-16.
[14]秦特夫,黄洛华.5种不同品系相思木材的化学性质Ⅰ:木材化学组成及差异性[J].林业科学研究,2005,18(2):191-194.