应用近红外光谱技术快速评价牛黄清胃丸质量
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摘要
目的:探讨近红外光谱技术在中药快速质量评价中的应用。方法:采集牛黄清胃丸的漫反射光谱,结合相似度匹配算法建立品种的真伪鉴别模型,结合偏最小二乘算法建立水分、果糖、葡萄糖、麦芽糖的多成分含量测定模型。结果:所有牛黄清胃丸样品相似度均高于90,模拟伪品相似度均低于90,定性分析模型误判数为0。水分、果糖、葡萄糖、麦芽糖的定量分析模型的预测均方差分别为0.65%、0.85%、1.04%、0.08%。结论:所建方法简便、快速、无损,可用于牛黄清胃丸的真伪鉴别,水分含量测定和辅料的质量控制,为近红外光谱技术在中药分析领域的应用提供了新的思路。
Objective:To explore application of near infrared spectroscopy(NIRS)in rapid quality evaluation of traditional Chinese medicine(TCM).Methods:Diffuse refle ctance spectra of Niuhuang Qingwei pills were collected,and model for sample identification was established by similarity match method,and multi-component determination of water,fructose,glucose and maltose were constructed with partial least square analogue.Results:The similarity values of all the Niuhuang Qingwei pills were above 90 and those of the simulated counterfeits were below 90.The results indicated that no misclassified sample was detected by qualitative model.And the root mean square error of prediction of the quantitative models for water,fructose,glucose and maltose were 0.65%,0.85%,1.04% and 0.08%,respectively.Conclusion:The proposed methods are simple,fast,non-destructive and applicable for authentication of Niuhuang Qingwei pills,water determination and quality control of excipients,which offer a new approach for application of NIRS in TCM analysis.
Objective:To explore application of near infrared spectroscopy(NIRS)in rapid quality evaluation of traditional Chinese medicine(TCM).Methods:Diffuse refle ctance spectra of Niuhuang Qingwei pills were collected,and model for sample identification was established by similarity match method,and multi-component determination of water,fructose,glucose and maltose were constructed with partial least square analogue.Results:The similarity values of all the Niuhuang Qingwei pills were above 90 and those of the simulated counterfeits were below 90.The results indicated that no misclassified sample was detected by qualitative model.And the root mean square error of prediction of the quantitative models for water,fructose,glucose and maltose were 0.65%,0.85%,1.04% and 0.08%,respectively.Conclusion:The proposed methods are simple,fast,non-destructive and applicable for authentication of Niuhuang Qingwei pills,water determination and quality control of excipients,which offer a new approach for application of NIRS in TCM analysis.
引文
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[6]聂黎行,张烨,胡晓茹,等.近红外光谱法结合模式识别技术快速无损鉴别天然牛黄、体外培育牛黄和人工牛黄[J].药物分析杂志,2017,37(10):1897NIE LX,ZHANG Y,HU XR,et al. Fast and non-destructive identification of Bovis Calculus,Bovis Calculus Sativus and Bovis Calculus Artifactus by near infrared spectroscopy combined with pattern recognition technology[J].Chin J Pharm Anal,2017,37(10):1897
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[13]孙逸威,李明华,宋丽丽.盐酸左氧氟沙星片的近红外光谱法快速检测[J].中国医药工业杂志,2018,49(6):814SUN YW,LI MH,SONG LL.Rapid detection of levofloxacin hydrochloride tablets with NIR spectroscopy[J].Chin J Pharm,2018,49(6):814
[14]闫研,秦斌,殷果,等.近红外光谱法在舒胸片快速定性定量分析中的应用[J].药学研究,2018,37(6):327YAN Y,QIN B,YIN G,et al. Rapid qualitative and quantitative analysis of Shuxiong tablets by near-infrared spectroscopic method[J].J Pharm Res,2018,37(6):327
[15]聂黎行,戴忠,鲁静,等.近红外光谱技术在活血止痛胶囊质量控制中的应用[J].药物分析杂志,2014,34(1):115NIE LX,DAI Z,LU J,et al. Application of near infrared spectroscopy in quality control of Huoxue Zhitong capsules[J].Chin J Pharm Anal,2014,34(1):115
[16]中华人民共和国药典2015年版.四部[S].2015:104ChP 2015.VolⅣ[S].2015:104
[17]何风艳,戴忠,聂黎行,等.牛黄清心丸(局方)中辅料蜂蜜的质量评价方法研究[J].药物分析杂志,2015,35(10):1833HE FY,DAI Z,NLE LX,et al. Study on quality evaluation of honey in Niuhuang Qingxin(Jufang)pills[J].Chin J Pharm Anal,2015,35(10):1833
[18]张玉荣,王瑞忠,鲁静.药用蜂蜜中5-羟甲基糠醛的限量检查方法研究[J].药物分析杂志,2018,38(2):326ZHANG YR,WANG RZ,LU J.Limit test of 5-hydroxymethyl furfural in medicinal honey[J].Chin J Pharm Anal,2018,38(2):326
[19]中华人民共和国药典2015年版.一部[S].2015:359ChP 2015.Vol I[S].2015:359
[20]邓勇,张杰良,宗凯,等.基于TLC和HPLC-CAD法比较分析天然和市售蜂蜜糖类成分[J].药物分析杂志,2018,38(1):22DENG Y,ZHANG JL,ZONG K,et al. Comparative analysis of carbohydrates in wild and commercial honey by using TLC and HPLC-CAD[J].Chin J Pharm Anal,2018,38(1):22
[21]李水芳,单杨,朱向荣,等.近红外透反射光谱用于检测蜂蜜加入麦芽糖浆掺假[J].食品科技,2010,35(12):299LI SF,SHAN Y,ZHU XR,et al. Detection of honey adulteration by addition of maltose syrup usingnear-infrared transflectance spectroscopy[J].Food Sci Technol,2010,35(12):299
[22]张欣.近红外和拉曼光谱法测定蜂蜜掺假研究[D].长沙:中南大学,2011ZHANG X.Studies on Discrimination of Honey Adulteration by Near Infrared and Raman Spec troscopy[D].Changsha:Central South University,2011
[2]HU CQ,FENG YC,YIN LH.Review of the characteristics and prospects of near infrared spectroscopy for rapid drug screening systems in China[J].J Near Infrared Spectrosc,2015,23(5):271
[3]赖添悦,蔡逢煌,彭昕,等.核密度估计算法结合近红外光谱技术鉴别三叶青产地[J].光谱学与光谱分析,2018,38(3):794LAI TY,CAI PH,PENG X,et al. Identification of Tetrastigma hemsleyanum from different places with FT-NIR combined with kernel density estimation algorithm[J].Spectrosc Spect Anal,2018,38(3):794
[4]冯艳春,胡昌勤.建立近红外方法用于阿莫仙胶囊流通环节的质量监测[J].中国药事,2016,30(11):1123FENG YC,HU CQ.Construction of the quality tracking method for Amoxian capsules in the circulation link by near infrared spectroscopy[J].Chin Pharm Aff,2016,30(11):1123
[5]耿颖,程奇蕾,何兰.运用近红外光谱法对缬沙坦胶囊进行一致性评价的研究[J].药物分析杂志,2015,35(1):161GENG Y,CHENG QL,HE L.Study on uniformity evaluation of valsartan capsules by near infrared spectroscopy[J].Chin J Pharm Anal,2015,35(1):161
[6]聂黎行,张烨,胡晓茹,等.近红外光谱法结合模式识别技术快速无损鉴别天然牛黄、体外培育牛黄和人工牛黄[J].药物分析杂志,2017,37(10):1897NIE LX,ZHANG Y,HU XR,et al. Fast and non-destructive identification of Bovis Calculus,Bovis Calculus Sativus and Bovis Calculus Artifactus by near infrared spectroscopy combined with pattern recognition technology[J].Chin J Pharm Anal,2017,37(10):1897
[7]刘振丙,高春洋,杨辉华,等.基于近红外光谱检测和平衡级联稀疏分类的药品鉴别方法[J].光谱学与光谱分析,2017,37(2):435LIU ZB,GAO CY,YANG HH,et al. Drug discrimination method based on near infrared reflectance spectrum and balance cascading sparse representation based classification[J].Spectrosc Spect Anal,2017,37(2):435
[8]伏为峰,杨辉华,刘振丙,等.基于深度信念网络与随机森林的药品鉴别方法[J].计算机仿真,2018,35(4):325FU WF,YANG HH,LIU ZB,et al. Medicine identifying method based on deep belief network and random forests[J].Comput Simul,2018,35(4):325
[9]NIE LX,DAI Z,MA SC.Enhanced accuracy of near-infrared spectroscopy for traditional Chinese medicine with competitive adaptive reweighted sampling[J].Anal Lett,2016,49(14):2259
[10]CHONG XM,ZOU WB,YAO SC,et al. Rapid analysis of the quality of amoxicillin and clavulanate potassium tablets using diffuse reflectance near-infrared spectroscopy[J].AAPS Pharm Sci Tech,2017,18(4):1311
[11]易夏,冯艳春,胡昌勤,等.近红外光谱法探索在线监测注射用五水头孢唑林钠干燥过程中的水分含量[J].沈阳药科大学学报,2017,34(7):558YI X,FENG YC,HU CQ,et al. Construction of quantitative model for on-line drying process endpoint detection of water in cefazolin sodium pentahydrate powder for injection using near infrared(NIR spectroscopy technology[J].J Shenyang:Pharm Univ,2017,34(7):558
[12]冯艳春,张琪,胡昌勤.药品近红外光谱通用性定量模型评价参数的选择[J].光谱学与光谱分析,2016,36(8):2447FENG YC,ZHANG Q,HU CQ.Study on the selection of parameters for evaluating drug NIR universal quantitative models[J].Spectrosc Spect Anal,2016,36(8):2447
[13]孙逸威,李明华,宋丽丽.盐酸左氧氟沙星片的近红外光谱法快速检测[J].中国医药工业杂志,2018,49(6):814SUN YW,LI MH,SONG LL.Rapid detection of levofloxacin hydrochloride tablets with NIR spectroscopy[J].Chin J Pharm,2018,49(6):814
[14]闫研,秦斌,殷果,等.近红外光谱法在舒胸片快速定性定量分析中的应用[J].药学研究,2018,37(6):327YAN Y,QIN B,YIN G,et al. Rapid qualitative and quantitative analysis of Shuxiong tablets by near-infrared spectroscopic method[J].J Pharm Res,2018,37(6):327
[15]聂黎行,戴忠,鲁静,等.近红外光谱技术在活血止痛胶囊质量控制中的应用[J].药物分析杂志,2014,34(1):115NIE LX,DAI Z,LU J,et al. Application of near infrared spectroscopy in quality control of Huoxue Zhitong capsules[J].Chin J Pharm Anal,2014,34(1):115
[16]中华人民共和国药典2015年版.四部[S].2015:104ChP 2015.VolⅣ[S].2015:104
[17]何风艳,戴忠,聂黎行,等.牛黄清心丸(局方)中辅料蜂蜜的质量评价方法研究[J].药物分析杂志,2015,35(10):1833HE FY,DAI Z,NLE LX,et al. Study on quality evaluation of honey in Niuhuang Qingxin(Jufang)pills[J].Chin J Pharm Anal,2015,35(10):1833
[18]张玉荣,王瑞忠,鲁静.药用蜂蜜中5-羟甲基糠醛的限量检查方法研究[J].药物分析杂志,2018,38(2):326ZHANG YR,WANG RZ,LU J.Limit test of 5-hydroxymethyl furfural in medicinal honey[J].Chin J Pharm Anal,2018,38(2):326
[19]中华人民共和国药典2015年版.一部[S].2015:359ChP 2015.Vol I[S].2015:359
[20]邓勇,张杰良,宗凯,等.基于TLC和HPLC-CAD法比较分析天然和市售蜂蜜糖类成分[J].药物分析杂志,2018,38(1):22DENG Y,ZHANG JL,ZONG K,et al. Comparative analysis of carbohydrates in wild and commercial honey by using TLC and HPLC-CAD[J].Chin J Pharm Anal,2018,38(1):22
[21]李水芳,单杨,朱向荣,等.近红外透反射光谱用于检测蜂蜜加入麦芽糖浆掺假[J].食品科技,2010,35(12):299LI SF,SHAN Y,ZHU XR,et al. Detection of honey adulteration by addition of maltose syrup usingnear-infrared transflectance spectroscopy[J].Food Sci Technol,2010,35(12):299
[22]张欣.近红外和拉曼光谱法测定蜂蜜掺假研究[D].长沙:中南大学,2011ZHANG X.Studies on Discrimination of Honey Adulteration by Near Infrared and Raman Spec troscopy[D].Changsha:Central South University,2011