甘草总黄酮对鸡产气荚膜梭菌坏死性肠炎的治疗作用
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摘要
选择具有显著抗炎效果的甘草总黄酮为受试化合物,考察其抗产气荚膜梭菌所致鸡坏死性肠炎的效果,为甘草总黄酮在坏死性肠炎的治疗应用提供依据。选择AA~+肉鸡,经口给予产气荚膜梭菌ATCC13124菌悬液建立肉仔鸡坏死性肠炎模型,之后使用甘草总黄酮对造模成功的肉鸡进行治疗,通过剖检考察肉鸡肠道的病变情况、病变评分、日增重及器官指数等指标以评估甘草总黄酮对坏死性肠炎的治疗效果。结果显示,通过经口攻菌成功建立了肉仔鸡坏死性肠炎模型;剖检发现,经甘草总黄酮治疗的模型鸡其肠道病变程度明显缓解,病变损伤评分显著低于感染组,炎性因子含量显著降低;治疗组肉鸡日增质量及器官指数与健康肉鸡差异不显著。结果表明,甘草总黄酮能够缓解产气荚膜梭菌所致坏死性肠炎对肉鸡肠道的损伤,具有作为抗生素替代品治疗坏死性肠炎的潜力。
The objective of this study is to investigate the protection effect of glycyrrhiza flavonoid,a known Chinese medicine extract with anti-inflammatory effect,on necrotic enteritis caused by Clostridium perfringens and to provide the theoretical basis for the application of glycyrrhiza flavonoid on necrotic enteritis treatment.Commercial Arbor Acres Plus broiler chickens were orally challenged with Clostridium perfringens ATCC13124 cultures to establish a model of necrotic enteritis.Infected chickens were treated with glycyrrhiza flavonoid.And on day 18,chickens were euthanased with cervical dislocation and their small intestines were examined for gross necrotic lesions to evaluate therapeutic effect of glycyrrhiza flavonoid on necrotic enteritis.The results showed that necrotic enteritis model was established successfully by oral administration of Clostridium perfringens ATCC13124 cultures.Pathological changes indicated that the intestinal lesions were significantly alleviated after treated by glycyrrhiza flavonoid.The lesion scores and inflammatory factor in treatment group were significantly lower compared with the positive group.There was no significant difference in body weight gain and organ index between broilers in the treatment group and healthy group.The study showed that glycyrrhiza flavonoid could alleviate the intestinal damage caused by necrotic enteritis and may have the potential to be an antibiotic alternatives for the treatment of necrotic enteritis caused by Clostridium perfringens.
The objective of this study is to investigate the protection effect of glycyrrhiza flavonoid,a known Chinese medicine extract with anti-inflammatory effect,on necrotic enteritis caused by Clostridium perfringens and to provide the theoretical basis for the application of glycyrrhiza flavonoid on necrotic enteritis treatment.Commercial Arbor Acres Plus broiler chickens were orally challenged with Clostridium perfringens ATCC13124 cultures to establish a model of necrotic enteritis.Infected chickens were treated with glycyrrhiza flavonoid.And on day 18,chickens were euthanased with cervical dislocation and their small intestines were examined for gross necrotic lesions to evaluate therapeutic effect of glycyrrhiza flavonoid on necrotic enteritis.The results showed that necrotic enteritis model was established successfully by oral administration of Clostridium perfringens ATCC13124 cultures.Pathological changes indicated that the intestinal lesions were significantly alleviated after treated by glycyrrhiza flavonoid.The lesion scores and inflammatory factor in treatment group were significantly lower compared with the positive group.There was no significant difference in body weight gain and organ index between broilers in the treatment group and healthy group.The study showed that glycyrrhiza flavonoid could alleviate the intestinal damage caused by necrotic enteritis and may have the potential to be an antibiotic alternatives for the treatment of necrotic enteritis caused by Clostridium perfringens.
引文
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[19] WANG Z,ZENG X,MO Y,et al.Identification and characterization of a bile salt hydrolase from Lactobacillus salivarius for development of novel alternatives to antibiotic growth promoters[J].Appl Environ Microbiol,2012,78(24):8795-8802.
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[22] DU E,GAN L,LI Z,et al.In vitro antibacterial activity of thymol and carvacrol and their effects on broiler chickens challenged with Clostridium perfringens[J].J Anim Sci Biotechnol,2015(6):58.
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[24] MA D,SHAN A,LI J,et al.Influence of an aqueous extract of Ligustrum lucidum and an ethanol extract of Schisandra chinensis on parameters of antioxidative metabolism and spleen lymphocyte proliferation of broilers[J].Arch Anim Nutr,2009,63(1):66-74.
[25] YANG R,WANG L Q,YUAN B C,et al.The Pharmacologicalactivities of licorice[J].Planta Med,2015,81(18):1654-1669.
[26] PASSAMONTI S,TERDOSLAVICH M,FRANCA R,et al.Bioavailability of flavonoids:a review of their membrane transport and the function of bilitranslocase in animal and plant organisms[J].Curr Drug Metab,2009,10(4):369-394.
[27] JIA T,QIAO J,GUAN D,et al.Anti-inflammatory effects of licochalcone a on IL-1 beta-stimulated human osteoarthritis chondrocytes[J].Inflammation,2017,40(6):1894-1902.
[28] CHEN X,LIU Z,MENG R,et al.Antioxidative and anticancer properties of licochalcone A from licorice[J].J Ethnopharmacol,2017,198:331-337.
[29] AIELLO F,ARMENTANO B,POLERA N,et al.From vegetable waste to new agents for potential health applications:antioxidant properties and effects of extracts,fractions and pinocembrin from glycyrrhiza glabra l.aerial parts on viability of five human cancer cell lines[J].J Agric Food Chem,2017,65(36):7944-7954.
[30] AIPIRE A,LI J,YUAN P,et al.Glycyrrhiza uralensis water extract enhances dendritic cell maturation and antitumor efficacy of HPV dendritic cell-based vaccine[J].Sci Rep,2017(7):43796.
[31] ZHANG Y,ZHANG L,ZHANG Y,et al.The protective role of liquiritin in high fructose-induced myocardial fibrosis via inhibiting NF-kappaB and MAPK signaling pathway[J].Biomed Pharmacother,2016,84:1337-1349.
[32] YANG X L,LIU D,BIAN K,et al.Study on in vitro anti-inflammatory activity of total flavonoids from glycyrrhizae radix et rhizoma and its ingredients[J].Zhongguo Zhongyao Zazhi,2013,38(1):99-104.
[33] FU Y,CHEN J,LI Y J,et al.Antioxidant and anti-inflammatory activities of six flavonoids separated from licorice[J].Food Chem,2013,141(2):1063-1071.
[2] STEVENS D L,ALDAPE M J,BRYANT A E.Life-threatening clostridial infections[J].Anaerobe,2012,18(2):254-259.
[3] PETIT L,GIBERT M,POPOFF M R.Clostridium perfringens:toxinotype and genotype[J].Trends Microbiol,1999,7(3):104-110.
[4] VAN IMMERSEEL F,ROOD J I,MOORE R J,et al.Rethinking our understanding of the pathogenesis of necrotic enteritis in chickens[J].Trends Microbiol,2009,17(1):32-36.
[5] WILLIAMS R B.Intercurrent coccidiosis and necrotic enteritis of chickens:rational,integrated disease management by maintenance of gut integrity[J].Avian Pathol,2005,34(3):159-180.
[6] BRENNAN J,BAGG R,BARNUM D,et al.Efficacy of narasin in the prevention of necrotic enteritis in broiler chickens[J].Avian Dis,2001,45(1):210-214.
[7] KALDHUSDAL M,SCHNEITZ C,HOFSHAGEN M,et al.Reduced incidence of Clostridium perfringens-associated lesions and improved performance in broiler chickens treated with normal intestinal bacteria from adult fowl[J].Avian Dis,2001,45(1):149-156.
[8] VAN IMMERSEEL F,DE BUCK J,PASMANS F,et al.Clostridium perfringens in poultry:an emerging threat for animal and public health[J].Avian Pathol,2004,33(6):537-549.
[9] DAHIYA J P,WILKIE D C,VAN KESSEL A G,et al.Potential strategies for controlling necrotic enteritis in broiler chickens in post-antibiotic era[J].Anim Feed Sci Technol,2006,129(1/2):60-88.
[10] 张利.甘草的药理作用及现代研究进展[J].中医临床研究,2014(10):147-148.
[11] 姜雪,孙森凤,王悦,等.甘草药理作用研究进展[J].化工时刊,2017(7):25-28.
[12] YANG R,YUAN B C,MA Y S,et al.The anti-inflammatory activity of licorice,a widely used Chinese herb[J].Pharm Biol,2017,55(1):5-18.
[13] ALFAJARO M M,KIM H J,PARK J G,et al.Anti-rotaviral effects of Glycyrrhiza uralensis extract in piglets with rotavirus diarrhea[J].Virol J,2012(9):310.
[14] KEYBURN A L,BOYCE J D,VAZ P,et al.NetB,a new toxin that is associated with avian necrotic enteritis caused by Clostridium perfringens[J].PLoS Pathog,2008,4(2):e26.
[15] CASTANON J I.History of the use of antibiotic as growth promoters in European poultry feeds[J].Poult Sci,2007,86(11):2466-2471.
[16] RUBIO L A,PEINADO M J,RUIZ R,et al.Correlations between changes in intestinal microbiota composition andperformance parameters in broiler chickens[J].J Anim Physiol Anim Nutr (Berl),2015,99(3):418-423.
[17] SONG J,XIAO K,KE Y L,et al.Effect of a probiotic mixture on intestinal microflora,morphology,and barrier integrity of broilers subjected to heat stress[J].Poult Sci,2014,93(3):581-588.
[18] ZHANG T,XIE J,ZHANG M,et al.Effect of a potential probiotics Lactococcus garvieae B301 on the growth performance,immune parameters and caecum microflora of broiler chickens[J].J Anim Physiol Anim Nutr (Berl),2016,100(3):413-421.
[19] WANG Z,ZENG X,MO Y,et al.Identification and characterization of a bile salt hydrolase from Lactobacillus salivarius for development of novel alternatives to antibiotic growth promoters[J].Appl Environ Microbiol,2012,78(24):8795-8802.
[20] ANONYE B O.General Commentary on:alternatives to antibiotic growth promoters in animals[J].Front Vet Sci,2016(3):74.
[21] DU E,WANG W,GAN L,et al.Effects of thymol and carvacrol supplementation on intestinal integrity and immune responses ofbroiler chickens challenged with Clostridium perfringens[J].J Anim Sci Biotechnol,2016(7):19.
[22] DU E,GAN L,LI Z,et al.In vitro antibacterial activity of thymol and carvacrol and their effects on broiler chickens challenged with Clostridium perfringens[J].J Anim Sci Biotechnol,2015(6):58.
[23] 杨玥熹.柠檬醛微胶囊制备、性质表征及其在控制家禽坏死性肠炎中的应用[D].江苏无锡:江南大学,2015.
[24] MA D,SHAN A,LI J,et al.Influence of an aqueous extract of Ligustrum lucidum and an ethanol extract of Schisandra chinensis on parameters of antioxidative metabolism and spleen lymphocyte proliferation of broilers[J].Arch Anim Nutr,2009,63(1):66-74.
[25] YANG R,WANG L Q,YUAN B C,et al.The Pharmacologicalactivities of licorice[J].Planta Med,2015,81(18):1654-1669.
[26] PASSAMONTI S,TERDOSLAVICH M,FRANCA R,et al.Bioavailability of flavonoids:a review of their membrane transport and the function of bilitranslocase in animal and plant organisms[J].Curr Drug Metab,2009,10(4):369-394.
[27] JIA T,QIAO J,GUAN D,et al.Anti-inflammatory effects of licochalcone a on IL-1 beta-stimulated human osteoarthritis chondrocytes[J].Inflammation,2017,40(6):1894-1902.
[28] CHEN X,LIU Z,MENG R,et al.Antioxidative and anticancer properties of licochalcone A from licorice[J].J Ethnopharmacol,2017,198:331-337.
[29] AIELLO F,ARMENTANO B,POLERA N,et al.From vegetable waste to new agents for potential health applications:antioxidant properties and effects of extracts,fractions and pinocembrin from glycyrrhiza glabra l.aerial parts on viability of five human cancer cell lines[J].J Agric Food Chem,2017,65(36):7944-7954.
[30] AIPIRE A,LI J,YUAN P,et al.Glycyrrhiza uralensis water extract enhances dendritic cell maturation and antitumor efficacy of HPV dendritic cell-based vaccine[J].Sci Rep,2017(7):43796.
[31] ZHANG Y,ZHANG L,ZHANG Y,et al.The protective role of liquiritin in high fructose-induced myocardial fibrosis via inhibiting NF-kappaB and MAPK signaling pathway[J].Biomed Pharmacother,2016,84:1337-1349.
[32] YANG X L,LIU D,BIAN K,et al.Study on in vitro anti-inflammatory activity of total flavonoids from glycyrrhizae radix et rhizoma and its ingredients[J].Zhongguo Zhongyao Zazhi,2013,38(1):99-104.
[33] FU Y,CHEN J,LI Y J,et al.Antioxidant and anti-inflammatory activities of six flavonoids separated from licorice[J].Food Chem,2013,141(2):1063-1071.