胸腺肽α1对乳腺癌MCF-7细胞凋亡的影响
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摘要
目的探究胸腺肽α1对乳腺癌MCF-7细胞凋亡的影响,为临床上对乳腺癌的治疗提供指导。方法采用体外实验,研究胸腺肽α1对人乳腺癌细胞MCF-7凋亡的影响。制备胸腺肽α1与小鼠脾细胞共培养上清,此共培养上清体外与MCF-7细胞作用48小时后,MTT法测定其对细胞增殖的抑制作用。结果 t_(0.05/2,6)=2.447,t=3.899,即t>t_(0.05/2,6),P<0.05,经过胸腺肽α1作用的乳腺癌MCF-7细胞生长受到抑制且两组差异具有统计学意义(P<0.05)。结论胸腺肽α1对乳腺癌MCF-7细胞的生长有抑制作用。
Objective To investigate the effect of thymic peptidesα1 on the apoptosis of breast cancer MCF-7 cells,in order to provide guidance for the therapy of breast cancer in clinic.MethodsIn vitro experiments were carried out to study the effect of thymic peptidesα1 on the apoptosis of human breast cancer cell MCF-7.Thymic peptidesα1 was cultured with the spleen cells of mice to obtained supernatant,after 48 hours of interaction with MCF-7 cells in vitro MTT assay was performed to determine the inhibitory effect on cell proliferation.Results The growth of breast cancer MCF-7 cells was inhibited and the difference was statistically significant(t=3.899,P<0.05).Conclusions The thymic peptidesα1 could inhibit the growth of MCF-7 cells originating from breast cancer.
Objective To investigate the effect of thymic peptidesα1 on the apoptosis of breast cancer MCF-7 cells,in order to provide guidance for the therapy of breast cancer in clinic.MethodsIn vitro experiments were carried out to study the effect of thymic peptidesα1 on the apoptosis of human breast cancer cell MCF-7.Thymic peptidesα1 was cultured with the spleen cells of mice to obtained supernatant,after 48 hours of interaction with MCF-7 cells in vitro MTT assay was performed to determine the inhibitory effect on cell proliferation.Results The growth of breast cancer MCF-7 cells was inhibited and the difference was statistically significant(t=3.899,P<0.05).Conclusions The thymic peptidesα1 could inhibit the growth of MCF-7 cells originating from breast cancer.
引文
[1]陈素华,马天江,张智慧.胸腺肽α1增强TAC方案新辅助化疗治疗乳腺癌疗效分析[J].中国医学工程,2015,23(6):195.
[2]郭婉婕,官海静,马爱霞.右丙亚胺预防乳腺癌患者蒽环类药物化疗所致心脏毒性的效果和经济性系统综述[J].中国新药杂志,2017,26(11):1328-1334.
[3]Kuss I,Hathawaya B,Ferris RL,et al.Decreased absolute counts of Tlymphocyte subsets and yileir relation to disease in squamous cell carinoma of the head and neck[J].Clin Cancer Res,2004,10(11):3755-3762.
[4]Konjevic G,Jovic V,Vuletic A,et al.CD69 on CD56+NK cells and response to chemoimmunotherapy in metestatic melanoma[J].Eur JChin Invest,2007,3(7):887-896.
[5]Hanna J,Mandelboim O.When Killers became helpers[J].Trends Immunol,2007,28(5):201-206.
[6]雷双根,余小芬,谢春伟,等.胸腺肽α1对乳腺癌新辅助化疗后免疫功能的影响[J].中国基层医药,2013,20(6):851-853.
[7]潘润存.从哲学角度对肿瘤发生发展与免疫逃逸的研究[J].中国免疫学杂志,2012,28(9):862-864.
[8]熊亮发,贾玲,刘春生,等.化疗联合胸腺肽α1治疗乳腺癌的基础与临床观察[J].中国医药,2010,5(4):357-358.
[9]许冬梅,邸秀珍.胸腺肽α1的作用机制及研究进展[J].中国民康医学,2014,26(2):79-82.
[2]郭婉婕,官海静,马爱霞.右丙亚胺预防乳腺癌患者蒽环类药物化疗所致心脏毒性的效果和经济性系统综述[J].中国新药杂志,2017,26(11):1328-1334.
[3]Kuss I,Hathawaya B,Ferris RL,et al.Decreased absolute counts of Tlymphocyte subsets and yileir relation to disease in squamous cell carinoma of the head and neck[J].Clin Cancer Res,2004,10(11):3755-3762.
[4]Konjevic G,Jovic V,Vuletic A,et al.CD69 on CD56+NK cells and response to chemoimmunotherapy in metestatic melanoma[J].Eur JChin Invest,2007,3(7):887-896.
[5]Hanna J,Mandelboim O.When Killers became helpers[J].Trends Immunol,2007,28(5):201-206.
[6]雷双根,余小芬,谢春伟,等.胸腺肽α1对乳腺癌新辅助化疗后免疫功能的影响[J].中国基层医药,2013,20(6):851-853.
[7]潘润存.从哲学角度对肿瘤发生发展与免疫逃逸的研究[J].中国免疫学杂志,2012,28(9):862-864.
[8]熊亮发,贾玲,刘春生,等.化疗联合胸腺肽α1治疗乳腺癌的基础与临床观察[J].中国医药,2010,5(4):357-358.
[9]许冬梅,邸秀珍.胸腺肽α1的作用机制及研究进展[J].中国民康医学,2014,26(2):79-82.