宁夏2017年HIV-1耐药毒株传播情况监测分析
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摘要
目的了解宁夏未经抗病毒治疗HIV感染人群的HIV耐药毒株传播情况。方法以世界卫生组织"HIV耐药警戒线监测方案"为指导,连续采集2017年7月-12月新确认的宁夏籍HIV感染者血浆样本,进行耐药基因型检测和耐药株传播水平分析。结果 35份样本完成了耐药及基因亚型鉴定,未发现针对蛋白酶抑制剂的相关突变;核苷类逆转录酶抑制剂相关突变株1例,突变位点为A62AV;非核苷类反转录酶抑制剂相关突变株4例,突变位点为V179T/E/VD。但以上突变位点均不是监测相关的突变位点。35例样本的HIV-1亚型为CRF07_BC、CRF01_AE和B亚型。结论宁夏仍属于HIV-1耐药株传播的低流行水平,应持续开展HIV耐药毒株传播的监测。
Objective To study the HIV-1 drug resistance transmission in Ningxia. Methods Referring to the guidelines for HIV drug resistance threshold survey(HIVDR-TS),plasma samples of the newly confirmed HIV infection in Ningxia were continuously collected from July to December in 2017 for drug resistance genotype detection and analysis of drug-resistant strains. Results Thirty five samples were conducted for drug resistance test and gene subtype identification,and no related mutations for protease inhibitors were found. The mutation of nucleoside analog reverse transcriptase inhibitors was found in 1 case,and the mutation site was A62 AV; 4 cases of non-nucleoside analog reverse transcriptase inhibitors related mutations were found,and the mutation site was V179 T/E/VD. However,all of the above mutation sites are not monitoring related mutation sites. The HIV-1 subtypes of 35 cases were CRF07_BC,CRF01_AE and B subtypes. Conclusion Ningxia is at to the low prevalence of HIV-1 resistant strains and should continue to monitor the transmission of HIV resistant strains.
Objective To study the HIV-1 drug resistance transmission in Ningxia. Methods Referring to the guidelines for HIV drug resistance threshold survey(HIVDR-TS),plasma samples of the newly confirmed HIV infection in Ningxia were continuously collected from July to December in 2017 for drug resistance genotype detection and analysis of drug-resistant strains. Results Thirty five samples were conducted for drug resistance test and gene subtype identification,and no related mutations for protease inhibitors were found. The mutation of nucleoside analog reverse transcriptase inhibitors was found in 1 case,and the mutation site was A62 AV; 4 cases of non-nucleoside analog reverse transcriptase inhibitors related mutations were found,and the mutation site was V179 T/E/VD. However,all of the above mutation sites are not monitoring related mutation sites. The HIV-1 subtypes of 35 cases were CRF07_BC,CRF01_AE and B subtypes. Conclusion Ningxia is at to the low prevalence of HIV-1 resistant strains and should continue to monitor the transmission of HIV resistant strains.
引文
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[11]佐合拉·吐尔地,廖玲洁,孙磊,等.新疆乌鲁木齐市2014年HIV-1耐药毒株传播分析[J].中国艾滋病性病,2016,22(8):586-588.
[12]苏玲,魏东兵,杨洪,等.四川省2014年人类免疫缺陷病毒1型耐药警戒线调查[J].中国病毒病杂志,2016,6(3):194-197.
[13]刘佳,闫江舟,孙定勇,等. 2015年河南省新确证HIV-1感染者耐药传播状况研究[J].河南医学研究,2018,27(4):598-601.
[14]羊海涛,肖占沛,还锡萍,等.江苏省HIV耐药警戒线的调查[J].南京医科大学学报,2012,32(1):1-4.
[15]黄丹,李玉锋,谭唯,等.深圳市2010年感染HIV的男男性接触者原发耐药基因突变监测[J].现代预防医学,2013,40(3):521-525.
[2]Little SJ,Holte S,Routy JP,et al. Antiretroviral-drug resistance among patients recently infected with HIV[J]. N Engl J Med,2002,347(6):385-394.
[3]刘大锦,冯孟贤,刘民.中国未接受抗病毒治疗的人类免疫缺陷病毒/获得性免疫缺陷综合征(HIV/AIDS)人群HIV原发耐药的Meta分析[J].北京大学学报:医学版,2015,47(3):474-482.
[4]Liu TF,Shafer RW. Web resources for HIV type 1 genotypic-resistance test interpretation[J]. Clin Infect Dis,2006,42(11):1608-1618.
[5]van Westen GJ,Hendriks A,Wegner JK,et al. Significantly improved HIV inhibitor efficacy prediction employing proteochemometric models generated from antivirogram data[J]. PLo S Comput Biol,2013,9(2):e1002899.
[6]Bennett DE,Camacho RJ,Otelea D,et al. Drug resistance mutations for surveillance of transmitted HIV-1 drug-resistance:2009 update[J]. PLo S ONE,2009,4(3):e4724.
[7]Bhargava M,Cajas JM,Wainberg MA,et al. Do HIV-1 non-B subtypes differentially impact resistance mutations and clinical disease progression in treated populations Evidence from a systematic review[J]. J International AIDS Society,2014,17:18944.
[8]殷玥琪,朱靖,吴楠楠,等.中国MSM人群中HIV-1亚型与不同位点耐药突变发生频率的Meta分析[J].中华疾病控制杂志,2017,21(6):622-626.
[9]吴忠兰,苟明星,关光玉,等.宁夏地区艾滋病患者感染HIV-1病毒pol区基因变异性研究[J].宁夏医科大学学报,2017,39(7):781-782.
[10]周全华,凌华,邢辉,等.重庆市HIV-1耐药警戒线调查[J].现代预防医学,2013,40(20):3860-3864.
[11]佐合拉·吐尔地,廖玲洁,孙磊,等.新疆乌鲁木齐市2014年HIV-1耐药毒株传播分析[J].中国艾滋病性病,2016,22(8):586-588.
[12]苏玲,魏东兵,杨洪,等.四川省2014年人类免疫缺陷病毒1型耐药警戒线调查[J].中国病毒病杂志,2016,6(3):194-197.
[13]刘佳,闫江舟,孙定勇,等. 2015年河南省新确证HIV-1感染者耐药传播状况研究[J].河南医学研究,2018,27(4):598-601.
[14]羊海涛,肖占沛,还锡萍,等.江苏省HIV耐药警戒线的调查[J].南京医科大学学报,2012,32(1):1-4.
[15]黄丹,李玉锋,谭唯,等.深圳市2010年感染HIV的男男性接触者原发耐药基因突变监测[J].现代预防医学,2013,40(3):521-525.