贵州鲜铁皮石斛粉小鼠急性经口毒性和骨髓红细胞微核试验研究
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摘要
目的研究贵州鲜铁皮石斛粉小鼠急性经口毒性和骨髓红细胞微核试验。方法用贵州鲜铁皮石斛粉采用限量法对实验小鼠进行急性经口毒性试验;骨髓红细胞微核试验选用昆明种小鼠50只(体重25～30 g),按体重随机分为5组,每组10只,雌雄各半;设置低、中、高剂量组(1.25、2.50、5.00 g/kg BW),阴性对照组和阳性对照组,间隔24 h两次灌胃,第2次灌胃后6 h颈椎脱臼处死动物,取胸骨骨髓用小牛血清稀释涂片,甲醇固定,Giemsa染色。结果急性经口毒性试验:试验观察14 d,在试验过程中和试验结束时,未观察到实验小鼠出现中毒症状和死亡;试验结束时对实验小鼠进行大体解剖,未见异常,LD50>15 g/kg BW。骨髓红细胞微核试验:鲜铁皮石斛粉各剂量组微核率与阴性对照组比较差异无统计学意义(P>0.05),阳性对照组与阴性对照组比较差异有统计学意义(P<0.05)。各剂量组、阴性对照组及阳性对照组嗜多染红细胞在总红细胞中的比例(PCE/RBC)均在正常值范围内。结论贵州鲜铁皮石斛粉属实际无毒范围,未观察到铁皮石斛粉能诱导有核红细胞染色体损伤或有丝分裂器损伤的遗传毒性。
Objective To study dendrobium officinale powder from Guizhou by acute oral toxicity test and micronucleus test of bone marrow erythrocyte.Methods Acute oral toxicity of Guizhou dendrobium officinale powder in mice was detected by maximum tolerated dose method. 50 Kunming mice weighing 25-30 g were randomly divided into 5 groups according to body weight with 10 in each, male and female in half. Micronucleus test was carried out in three dose groups(1.25, 2.50 and 5.00 g/kgBW), negative control group and positive control group.Intragastric administration were conducted twice with the interval of 24 h. The mice were killed by cervical dislocation after 6 hours. Dilution of sternal bone marrow smeared with calf serum. Slides were stained by Giemsa after fixed with methanol.Results The acute oral LD50 in mice was over 15 g/kg BW, mice had no obvious poisoning symptoms and death during continuous observation for 14 d. No abnormal changes of important organs were found in all mice. Compared with negative control group, the micronucleus rate of dendrobium officinale powder was not statisticallly significant(P>0.05). The difference between positive control group and negative control group was statistically significiant(P<0.05). PCE/RBC in every dose group were inside the normal range.Conclusion Dendrobium officinale powder is actually nontoxic. NO damage of chromosomes in nucleated red blood cells and mitotic apparatus are induced in this test dose range, dendrobium officinale powder do not show genotoxicity.
Objective To study dendrobium officinale powder from Guizhou by acute oral toxicity test and micronucleus test of bone marrow erythrocyte.Methods Acute oral toxicity of Guizhou dendrobium officinale powder in mice was detected by maximum tolerated dose method. 50 Kunming mice weighing 25-30 g were randomly divided into 5 groups according to body weight with 10 in each, male and female in half. Micronucleus test was carried out in three dose groups(1.25, 2.50 and 5.00 g/kgBW), negative control group and positive control group.Intragastric administration were conducted twice with the interval of 24 h. The mice were killed by cervical dislocation after 6 hours. Dilution of sternal bone marrow smeared with calf serum. Slides were stained by Giemsa after fixed with methanol.Results The acute oral LD50 in mice was over 15 g/kg BW, mice had no obvious poisoning symptoms and death during continuous observation for 14 d. No abnormal changes of important organs were found in all mice. Compared with negative control group, the micronucleus rate of dendrobium officinale powder was not statisticallly significant(P>0.05). The difference between positive control group and negative control group was statistically significiant(P<0.05). PCE/RBC in every dose group were inside the normal range.Conclusion Dendrobium officinale powder is actually nontoxic. NO damage of chromosomes in nucleated red blood cells and mitotic apparatus are induced in this test dose range, dendrobium officinale powder do not show genotoxicity.
引文
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[8] 阮敏慧,陈方亮,潘立兵.铁皮石斛冲剂的急性毒性及致突变性[J].现代医药卫生,2002,18(7):556-557.
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[10] 张立实,李宁.食品毒理学[M].北京:科学出版社,2017:149-188.
[2] 叶飞飞,胡雪华,李晓红,等.铁皮石斛研究进展[J].农技服务,2016,33(4):18-19.
[3] 辛甜,储智勇,栾洁,等.铁皮石斛胚状体对大鼠抗疲劳能力的影响[J].药学实践杂志,2011,29(1):21-23.
[4] 吴建,王建方,方玲,等.国内铁皮石斛研究概况[J].中国药学杂志,2013,48(19):1610-1613.
[5] Xing XH,Cui SW,Nie SP,et al. A review of isolation process, structural characteristics, and bioactivities of water-soluble polysaccharides from Dendrobium plants [J]. Bioact Carbohydrates and Dietary Fibre, 2013,1(2):131-147.
[6] 傅剑云,夏勇,徐彩菊,等.铁皮石斛提取液的毒性研究及安全性评价[J].浙江预防医学,1998(4):250-251.
[7] 谷芳芳,周夏慧,朱晓静,等.鲜铁皮石斛遗传毒性实验研究[J] .中国新药杂志,2016,25(21):2506-2509.
[8] 阮敏慧,陈方亮,潘立兵.铁皮石斛冲剂的急性毒性及致突变性[J].现代医药卫生,2002,18(7):556-557.
[9] 温平镜,韦小敏,李彬,等.铁皮石斛胶囊毒理学安全性实验研究[J] .应用预防医学,2013,19(5):313-315.
[10] 张立实,李宁.食品毒理学[M].北京:科学出版社,2017:149-188.