红颜草莓组培快繁体系优化
|
摘要
为进一步优化红颜草莓组培快繁体系,以红颜草莓匍匐茎尖为外植体,筛选最佳灭菌时间,并通过正交试验设计,研究培养基及植物生长调节剂对不定芽诱导、增殖、生根的影响。结果显示:最佳灭菌处理为75%酒精消毒30s,0.1%HgCl2消毒7min,成活率可达90%。对芽诱导的影响作用为基本培养基>6-BA>IBA>NAA,筛选出最佳不定芽诱导培养基为MS+6-BA2.0mg·L~(-1)+NAA0.3mg·L~(-1)+IBA0.3mg·L~(-1),诱导率92%。增殖阶段3种植物生长调节剂对试管苗的影响力为6-BA>GA3>IBA,最佳增殖培养基为MS+6-BA0.50mg·L~(-1)+IBA0.08mg·L~(-1)+GA30.10mg·L~(-1),增殖系数13.97。生根培养阶段,对生根指数的影响力为基本培养基>IBA>蔗糖,最佳生根培养基为1/2MS+蔗糖30g·L~(-1)+IBA0.4g·L~(-1),生根指数为35.40。最佳移栽基质为草炭土∶珍珠岩=1∶1,成活率为93.33%。本试验采用的植物生长调节剂种类及浓度进一步提高了红颜草莓组培诱导率和增殖率,获得了良好的生根指数和移栽成活率,进一步优化了红颜草莓的快繁体系。
To optimize the tissue culture and rapid propagation of Benihoppe strawberry,effects of basic medium and plant growth regulators on the induction,proliferation and rooting of the adventitious buds were studied.The results showed that the disinfection of the strawberry stem tips with 75% alcohol for 30 sand 0.1% HgCl_2 for 7 min satisfactorily rendered a survival rate of the tissue up to 90%.The bud induction was affected by the following factors in the order of basic medium>6-BA>IBA>NAA.The best medium for the highest induction rate of 92%consisted of MS+6-BA 2.0 mg·L~(-1)+NAA 0.3 mg·L~(-1)+IBA 0.3 mg·L~(-1).The effects of the 3 plant growth regulators in the proliferation stage ranked 6-BA>GA3>IBA with the optimal medium containing MS+6-BA 0.50 mg·L~(-1)+IBA 0.08 mg·L~(-1)+GA30.10 mg·L~(-1) resulting in a proliferation coefficient of 13.97.For the rooting,the effects were found to be in the order of basic medium>IBA>sucrose,and the optimal medium to be 1/2 MS+sucrose 30 g·L~(-1)+IBA 0.4 g·L~(-1) to yield a rooting index of 35.40.The best basic medium was peat∶perlite at1∶1 ratio that provided a seedling survival rate of 93.33%.The plant growth regulator and concentration used in this experiment further improved the induction and proliferation of Benihoppe strawberry,and obtained a good rooting index and seedling survival rate,and further optimized the rapid propagation system of Benihoppe strawberry.
To optimize the tissue culture and rapid propagation of Benihoppe strawberry,effects of basic medium and plant growth regulators on the induction,proliferation and rooting of the adventitious buds were studied.The results showed that the disinfection of the strawberry stem tips with 75% alcohol for 30 sand 0.1% HgCl_2 for 7 min satisfactorily rendered a survival rate of the tissue up to 90%.The bud induction was affected by the following factors in the order of basic medium>6-BA>IBA>NAA.The best medium for the highest induction rate of 92%consisted of MS+6-BA 2.0 mg·L~(-1)+NAA 0.3 mg·L~(-1)+IBA 0.3 mg·L~(-1).The effects of the 3 plant growth regulators in the proliferation stage ranked 6-BA>GA3>IBA with the optimal medium containing MS+6-BA 0.50 mg·L~(-1)+IBA 0.08 mg·L~(-1)+GA30.10 mg·L~(-1) resulting in a proliferation coefficient of 13.97.For the rooting,the effects were found to be in the order of basic medium>IBA>sucrose,and the optimal medium to be 1/2 MS+sucrose 30 g·L~(-1)+IBA 0.4 g·L~(-1) to yield a rooting index of 35.40.The best basic medium was peat∶perlite at1∶1 ratio that provided a seedling survival rate of 93.33%.The plant growth regulator and concentration used in this experiment further improved the induction and proliferation of Benihoppe strawberry,and obtained a good rooting index and seedling survival rate,and further optimized the rapid propagation system of Benihoppe strawberry.
引文
[1]方博云,费鸣毅,朱小富,等.草莓新品种——红颊[J].上海蔬菜,2004(4):21-22.
[2]李会珍,徐东进,陈登金,等.不同植物生长调节剂对脱毒红颊草莓组培快繁的影响[J].江苏农业科学,2013,41(2):43-45.
[3]郭月玲,解振强,王永平.我国草莓组织培养生产研究现状及前景[J].浙江农业科学,2010(6):1211-1215.
[4]张黎凤,钱雯婕,魏红英,等.红颜草莓茎尖组培快繁技术研究[J].现代农业科技,2017(7):71-74.
[5]朱海生,李永平,林珲,等.草莓品种的亲缘关系及其不同继代次数的叶片组培苗的RAPD分析[J].核农学报,2010,24(2):264-268.
[6]马崇坚,廖佩颖.草莓叶柄和叶片的组织培养[J].安徽农业科学,2007(13):3841-3842.
[7]何欢乐,阳静,蔡润,等.草莓茎尖培养脱毒效果研究[J].北方园艺,2005(5):79-81.
[8]董敬超.红颜草莓茎尖组织培养快繁技术研究[J].北方园艺,2013(24):106-108.
[9]王晓玲,唐丽萍,范雨,等.红颜草莓茎尖组织培养的器官发生途径研究[J].四川林业科技,2017,38(6):40-44.
[10]李卫东,葛会波,周春江,等.草莓花药愈伤组织类型与状态调控研究[J].河北农业大学学报,2004(2):59-63.
[11]成晓华,杜国强,师校欣.甜查理和红颜草莓组培苗根系发育影响因子分析[J].江苏农业科学,2017,45(24):38-40.
[12]罗君琴,李丽,林俊,等.“红颊”草莓茎尖快繁技术研究[J].现代园艺,2008(11):4-5.
[13]刘彤,赵新俊,任丽彤,等.新疆香梨试管苗最佳生根培养基研究[J].果树学报,2004(2):124-127.
[14]田芳,王晓云,李晓青,等.草莓章姬组培快繁技术研究[J].农业科技通讯,2018(4):196-197.
[15]吴鹏飞,王丽娟,切岩祥,等.设施草莓组培快繁研究现状分析[J].北方园艺,2016(1):195-199.
[16]孙瑞芬,李天然,李堃,等.草莓组培快繁及叶片诱导植株再生的研究[J].华北农学报,2002(4):49-53.
[17]邓馨,胡文云.草莓叶片再生芽及遗传转化系统的建立[J].植物学通报,2000,17(2):174-178.
[18]陈英,张西英.草莓品种红颜组培快繁体系的优化[J].新疆农业科学,2016,53(12):2210-2216.
[19]翟婷婷,刘成连,原永兵,等.草莓茎尖培养快繁体系的研究[J].安徽农业大学学报,2015,42(4):545-548.
[20]李晓亮,张军云,张钟,等.草莓茎尖组织培养和快繁体系的建立[J].作物杂志,2016(4):68-74,173.
[21]郭靖,顾虹,窦祖霞.草莓组培苗生根培养技术研究[J].现代农业科技,2012(20):88.
[22]王玮玮,汪国莲,孙玉东,等.不同浓度激素、活性炭对红颜草莓茎尖组培的影响[J].江苏农业科学,2015,43(8):46-48.
[23]薛其勤,李美芹,吕金浮,等.不同基因型优质草莓组织培养快繁研究[J].北方园艺,2014(21):110-113.
[24]刘根林,梁珍海,朱军.活性炭在植物组织培养中的作用概述[J].江苏林业科技,2001(5):46-48.
[25]梁凤龙.几种草莓优良品种的组培快繁和脱毒苗生产技术研究[J].现代园艺,2013(20):23,25.
[2]李会珍,徐东进,陈登金,等.不同植物生长调节剂对脱毒红颊草莓组培快繁的影响[J].江苏农业科学,2013,41(2):43-45.
[3]郭月玲,解振强,王永平.我国草莓组织培养生产研究现状及前景[J].浙江农业科学,2010(6):1211-1215.
[4]张黎凤,钱雯婕,魏红英,等.红颜草莓茎尖组培快繁技术研究[J].现代农业科技,2017(7):71-74.
[5]朱海生,李永平,林珲,等.草莓品种的亲缘关系及其不同继代次数的叶片组培苗的RAPD分析[J].核农学报,2010,24(2):264-268.
[6]马崇坚,廖佩颖.草莓叶柄和叶片的组织培养[J].安徽农业科学,2007(13):3841-3842.
[7]何欢乐,阳静,蔡润,等.草莓茎尖培养脱毒效果研究[J].北方园艺,2005(5):79-81.
[8]董敬超.红颜草莓茎尖组织培养快繁技术研究[J].北方园艺,2013(24):106-108.
[9]王晓玲,唐丽萍,范雨,等.红颜草莓茎尖组织培养的器官发生途径研究[J].四川林业科技,2017,38(6):40-44.
[10]李卫东,葛会波,周春江,等.草莓花药愈伤组织类型与状态调控研究[J].河北农业大学学报,2004(2):59-63.
[11]成晓华,杜国强,师校欣.甜查理和红颜草莓组培苗根系发育影响因子分析[J].江苏农业科学,2017,45(24):38-40.
[12]罗君琴,李丽,林俊,等.“红颊”草莓茎尖快繁技术研究[J].现代园艺,2008(11):4-5.
[13]刘彤,赵新俊,任丽彤,等.新疆香梨试管苗最佳生根培养基研究[J].果树学报,2004(2):124-127.
[14]田芳,王晓云,李晓青,等.草莓章姬组培快繁技术研究[J].农业科技通讯,2018(4):196-197.
[15]吴鹏飞,王丽娟,切岩祥,等.设施草莓组培快繁研究现状分析[J].北方园艺,2016(1):195-199.
[16]孙瑞芬,李天然,李堃,等.草莓组培快繁及叶片诱导植株再生的研究[J].华北农学报,2002(4):49-53.
[17]邓馨,胡文云.草莓叶片再生芽及遗传转化系统的建立[J].植物学通报,2000,17(2):174-178.
[18]陈英,张西英.草莓品种红颜组培快繁体系的优化[J].新疆农业科学,2016,53(12):2210-2216.
[19]翟婷婷,刘成连,原永兵,等.草莓茎尖培养快繁体系的研究[J].安徽农业大学学报,2015,42(4):545-548.
[20]李晓亮,张军云,张钟,等.草莓茎尖组织培养和快繁体系的建立[J].作物杂志,2016(4):68-74,173.
[21]郭靖,顾虹,窦祖霞.草莓组培苗生根培养技术研究[J].现代农业科技,2012(20):88.
[22]王玮玮,汪国莲,孙玉东,等.不同浓度激素、活性炭对红颜草莓茎尖组培的影响[J].江苏农业科学,2015,43(8):46-48.
[23]薛其勤,李美芹,吕金浮,等.不同基因型优质草莓组织培养快繁研究[J].北方园艺,2014(21):110-113.
[24]刘根林,梁珍海,朱军.活性炭在植物组织培养中的作用概述[J].江苏林业科技,2001(5):46-48.
[25]梁凤龙.几种草莓优良品种的组培快繁和脱毒苗生产技术研究[J].现代园艺,2013(20):23,25.