GenoType MTBDRplus快速检测耐多药结核菌的应用评价
|
摘要
目的评估GenoType MTBDRplus(对结核杆菌复合群利福平和/或异烟肼耐药的分子诊断试剂盒)快速检测耐多药结核病的临床效果。方法收集河南省胸科医院2010年3月-2012年3月收治的结核患者150例,纳入者连续3 d提供痰标本,对每个痰标本进行抗酸杆菌涂片检测,改良罗氏(L-J)固体和MGIT960液体培养,培养阳性菌株采用L-J比例法、MGIT960液体法、GenoType MTBDRplus三种不同方法进行一线抗结核药物敏感性检测,对同一过程的不同试验方法的检测结果两两对比分析。结果 GenoType MTBDRplus检测耐多药结核菌需时短,与L-J比例法、MGIT960液体法对比,三种不同的药敏试验方法对相同菌株的药敏结果一致性两两对比分析差异无统计学意义(P>0.05)。耐多药检测符合率90%。结论GenoType MTBDRplus可用于耐多药结核菌(MDR-TB)的快速检测,满足临床对MDR-TB进行早期诊断和及时治疗的需要。
Objective To evaluate the clinical effectiveness of GenoType MTBDRplus for rapid detection of multi-drug resistant tuberculosis.Methods One hundred and fifty tuberculosis cases were collected from March 2010 to March 2012 in Henan Chest Hospital,and sputum samples were collected from these cases for three consecutive days for acid-fast bacillus smear detection.Then the positive strains were cultured by modified( L-J) solid and MGIT960 liquid culture method,and culture-positive strains were detected MDR-TB by L-J ratio method,MGIT960 liquid method and GenoType MTBDRplus method.Results GenoType MTBDRplus was rapid in detecting multidrug-resistant tuberculosis,when compared to L-J proportion method and MGIT960 method.Results of three kinds of different drug susceptibility testing methods were consistent on the same strain,with the coincidence rate of 90%.Conclusion GenoType MTBDRplus can be used for the rapid detection of MDR-TB,enough for the clinical need of early diagnosis and timely treatment.
Objective To evaluate the clinical effectiveness of GenoType MTBDRplus for rapid detection of multi-drug resistant tuberculosis.Methods One hundred and fifty tuberculosis cases were collected from March 2010 to March 2012 in Henan Chest Hospital,and sputum samples were collected from these cases for three consecutive days for acid-fast bacillus smear detection.Then the positive strains were cultured by modified( L-J) solid and MGIT960 liquid culture method,and culture-positive strains were detected MDR-TB by L-J ratio method,MGIT960 liquid method and GenoType MTBDRplus method.Results GenoType MTBDRplus was rapid in detecting multidrug-resistant tuberculosis,when compared to L-J proportion method and MGIT960 method.Results of three kinds of different drug susceptibility testing methods were consistent on the same strain,with the coincidence rate of 90%.Conclusion GenoType MTBDRplus can be used for the rapid detection of MDR-TB,enough for the clinical need of early diagnosis and timely treatment.
引文
[1]刘家云,徐修礼,孙惠平,等.耐药结核分支杆菌基因突变分析[J].中华检验医学杂志,2010,33(7):594-598.
[2]World Health organization.Anti-tuberculosis drug resistance in the world/the WHO/IUATLD Global Project on Anti-Tuberculosis Drug Resistance Surveillance.Report 2,Prevalence and trends[M].Geneva:WHO,2000.
[3]中国防痨协会基础专业委员会.结核病诊断细菌学检验规程[M].北京:中国教育文化出版社,2006:7-56.
[4]中国疾病预防控制中心.痰涂片质量保证手册[M].北京:中国协和医科大学出版社,2004:8-10.
[5]World Health organization.Guidelines for surveillance of drug resistant in tuberculosis[M].Geneva:WHO,2009.
[6]World Health organization.Guidelines for the programmatic management of drug-resistant tuberculosis[M].Geneva:WHO,2006:38-52.
[7]欧喜超,赵雁林.结核病实验室诊断及存在的难题[J].中华检验医学杂志,2011,34(2):97-100.
[8]秦晓华,马志承,沈鑫,等.embB 306位点作为耐多药结核分枝杆菌分子间的标记初探[J].中华检验医学杂志,2009,32(2):153-156.
[2]World Health organization.Anti-tuberculosis drug resistance in the world/the WHO/IUATLD Global Project on Anti-Tuberculosis Drug Resistance Surveillance.Report 2,Prevalence and trends[M].Geneva:WHO,2000.
[3]中国防痨协会基础专业委员会.结核病诊断细菌学检验规程[M].北京:中国教育文化出版社,2006:7-56.
[4]中国疾病预防控制中心.痰涂片质量保证手册[M].北京:中国协和医科大学出版社,2004:8-10.
[5]World Health organization.Guidelines for surveillance of drug resistant in tuberculosis[M].Geneva:WHO,2009.
[6]World Health organization.Guidelines for the programmatic management of drug-resistant tuberculosis[M].Geneva:WHO,2006:38-52.
[7]欧喜超,赵雁林.结核病实验室诊断及存在的难题[J].中华检验医学杂志,2011,34(2):97-100.
[8]秦晓华,马志承,沈鑫,等.embB 306位点作为耐多药结核分枝杆菌分子间的标记初探[J].中华检验医学杂志,2009,32(2):153-156.