河南省猪肺炎支原体感染的流行情况调查
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摘要
为了对河南省猪肺炎支原体的流行情况进行调查,试验从发生呼吸道疾病的肺中分离鉴定猪肺炎支原体,对疑似猪肺炎支原体的肺组织样品通过液体培养传代、固体培养基培养、吉姆萨染色镜检,PCR扩增,并进行测序比对。结果表明,2017年1—12月从河南省猪场发生呼吸困难的猪的实变的肺脏共采集148份,共分离25株猪肺炎支原体,分离率达16.89%。结果说明猪肺炎支原体在河南省发生呼吸道疾病的猪场中感染率较高。
To investigate the prevalence of Mycoplasma hyopneumoniae in Henan province,Mycoplasma hyopneumoniae were isolated and identified from the lungs of respiratory disease.Suspected swine Mycoplasma hyopneumonia lung tissue were cultured by liquid and solid medium,Giemsa staining,PCR amplified,sequenced and blasted.The results showed that from January to December2017,a total of 148 samples from the lungs of dyspnea pigs in Henan province were collected.25 strains Mycoplasma pneumoniae were isolated and identified.The isolation rate was 16.89%.The results explained that the infection rate of Mycoplasma hyopneumoniae was high in swine farms with respiratory diseases in Henan province.
To investigate the prevalence of Mycoplasma hyopneumoniae in Henan province,Mycoplasma hyopneumoniae were isolated and identified from the lungs of respiratory disease.Suspected swine Mycoplasma hyopneumonia lung tissue were cultured by liquid and solid medium,Giemsa staining,PCR amplified,sequenced and blasted.The results showed that from January to December2017,a total of 148 samples from the lungs of dyspnea pigs in Henan province were collected.25 strains Mycoplasma pneumoniae were isolated and identified.The isolation rate was 16.89%.The results explained that the infection rate of Mycoplasma hyopneumoniae was high in swine farms with respiratory diseases in Henan province.
引文
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[15]宫晓炜,马文瑛,陈启伟,等.畜禽支原体耐药性及耐药机制研究进展[J].中国畜牧兽医,2017,44(8):2489-2495.
[2]CVJETKOVIC V,SIPOS S,SZABO I,et al.Clinical efficacy of two vaccination stategies against Mycoplasma hyopneumoniae in a pig herd suffering from respiratory disease[J].Porcine Health Manag,2018,4:19.
[3]熊祺琰,范志鹏,甘源,等.几种佐剂对肌肉注射猪支原体肺炎活疫苗免疫刺激能力的增强作用研究[J].中国畜牧兽医,2011,38(10):163-168.
[4]施慈,刘洪斌,张凤强,等.猪支原体肺炎及其检测和防制的研究进展[J].中国农学通报,2014,30(17):14-20.
[5]田瑞雨,熊祺琰,刘茂军,等.猪肺炎支原体抗原定量ELISA检测方法的建立[J].中国兽医科学,2017,47(4):471-475.
[6]廖启顺,李志祥,丁联成,等.2009-2010年部分猪场猪主要传染病流行病学调查[J].中国畜牧兽医,2012,39(2):194-199.
[7]武昱孜,熊祺琰,刘蓓蓓,等.猪肺炎支原体和猪鼻支原体双重荧光定量PCR检测方法的建立[J].畜牧兽医学报,2017,48(8):1491-1498.
[8]张勋,吴鹏,李世震,等.新疆地区4种猪病多重PCR检测方法的建立与初步应用[J].中国畜牧兽医,2015,42(10):2612-2618.
[9]LUNG O,OHENE-ADJEI S,BUCHANAN C,et al.Multiplex PCRand microarray for detection of swine respiratory pathogens[J].Transbound Emerg Dis,2017,64(3):834-848.
[10]李海利,邓祖丽颖,徐引弟,等.猪肺炎支原体与猪鼻支原体分离鉴定及鉴别检测[J].河南农业科学,2017,46(11):148-151.
[11]彭凌,刘博婷,杨旭夫.猪鼻支原体套式PCR诊断方法的建立及应用[J].河南农业科学,2017,46(4):121-123.
[12]李颖平.间接免疫荧光抗体技术检测猪肺炎支原体[J].山西农业科学,2016,44(11):1702-1703,1707.
[13]车艳杰,于霏,高玉梅,等.猪肺炎支原体DJ-166株的分离鉴定[J].中国兽药杂志,2016,51(3):6-10.
[14]候凤,李新苹,王钢,等.3种支原体培养基对猪肺炎支原体CJ株培养效果的比较分析[J].中国兽药杂志,2016,51(3):21-23.
[15]宫晓炜,马文瑛,陈启伟,等.畜禽支原体耐药性及耐药机制研究进展[J].中国畜牧兽医,2017,44(8):2489-2495.