食品微生物实验室菌种的管理
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摘要
菌种是食品微生物实验室检验工作中不可缺少的重要生物资源,常用于培养基验收、试验对照、人员培训考核、方法确认等方面的质量控制。为实现菌种的规范化管理,保证实验室检验过程中质量控制活动的有效性,本文从人、机、料、法、环等方面对食品微生物实验室菌种管理的重点、难点进行探讨,总结出菌种管理全流程的关键控制点,保证了菌种来源可靠性及溯源性,保障了实验结果准确性。
The strain is an indispensable and important biological resource in the laboratory inspection of food microbiology, usually used for acceptance of medium, test control, personnel training assessment and method validation. In order to achieve standardized management of strains and ensure the effectiveness of quality control activities in the laboratory inspection process, this paper explores the difficult and key points in the management of food microbial laboratory strains from the aspects of human, machine,material, method, and environment. Summarizing the key control points of the whole process of strains management to ensure the reliability and traceability of strain source and the accuracy of experimental results.
The strain is an indispensable and important biological resource in the laboratory inspection of food microbiology, usually used for acceptance of medium, test control, personnel training assessment and method validation. In order to achieve standardized management of strains and ensure the effectiveness of quality control activities in the laboratory inspection process, this paper explores the difficult and key points in the management of food microbial laboratory strains from the aspects of human, machine,material, method, and environment. Summarizing the key control points of the whole process of strains management to ensure the reliability and traceability of strain source and the accuracy of experimental results.
引文
[1]张继忠.关于食品微生物检测问题的分析[J].科技创新与应用,2018(2):177-178.
[2]CNAS-CL01-A001 2018检测和校准实验室能力认可准则在微生物检测领域的应用说明[S].
[3]SN/T 1538.1-2016培养基制备指南[S].
[4]杨丰旭.标准菌种在食品微生物检验中的重要作用[J].食品安全导刊,2017(6):96.
[5]黄琴,李伟,卢明枝.浅谈实验室标准菌种的管理[J].中国检验检测,2018,26(1):59-60+14.
[6]闵航.微生物学[M].杭州:浙江大学出版社,2011.
[7]SN/T 2660-2010食品微生物实验室菌种保藏方法[S].
[8]GB/T 27405-2008实验室质量控制规范食品微生物检测[S].
[9]钟志宏,李启星,唐小异,等.甘油保存菌种方法的改良[J].实用预防医学,2005(3):670-671.
[10]SN/T 2632-2010微生物菌种常规保藏技术规程[S].
[11]潘新南.卫生检验的标准菌种管理要点[J].中国卫生检验杂志,2005(7):858-859.
[2]CNAS-CL01-A001 2018检测和校准实验室能力认可准则在微生物检测领域的应用说明[S].
[3]SN/T 1538.1-2016培养基制备指南[S].
[4]杨丰旭.标准菌种在食品微生物检验中的重要作用[J].食品安全导刊,2017(6):96.
[5]黄琴,李伟,卢明枝.浅谈实验室标准菌种的管理[J].中国检验检测,2018,26(1):59-60+14.
[6]闵航.微生物学[M].杭州:浙江大学出版社,2011.
[7]SN/T 2660-2010食品微生物实验室菌种保藏方法[S].
[8]GB/T 27405-2008实验室质量控制规范食品微生物检测[S].
[9]钟志宏,李启星,唐小异,等.甘油保存菌种方法的改良[J].实用预防医学,2005(3):670-671.
[10]SN/T 2632-2010微生物菌种常规保藏技术规程[S].
[11]潘新南.卫生检验的标准菌种管理要点[J].中国卫生检验杂志,2005(7):858-859.