枸杞对果蝇的抗衰老作用
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摘要
目的:利用动物模型黑腹果蝇研究枸杞的抗衰老作用。方法:将枸杞原液100 mL分别稀释1倍、3倍、5倍,制备基础培养基和试验用培养基。收集8 h内羽化未交配的黑腹果蝇,用乙醚麻醉后按雌雄随机进行分组,每个浓度选择雌果蝇和雄果蝇各20只,分别装在2个培养基中,每管10只。即每个浓度4个试管,置于(20±1)℃恒温培养箱中,每3 d更换1次培养基,分别进行果蝇寿命、性活力、飞翔能力的测定,探究枸杞的抗衰老作用。结果:3倍剂量组枸杞显著提高了果蝇的平均寿命(雄果蝇增加25.1%,雌果蝇增加27.5%,P<0.01)和最高寿命(雄果蝇增加27.3%,雌果蝇增加31.7%,P<0.01);果蝇的性活力显著提高13.5%(P<0.01)。结论:研究结果表明,枸杞在一定的浓度范围内对果蝇有显著的抗衰老作用。
Objective:To study the anti-aging effect of Fructus Lyciion on Drosophila melangaster. Methods:The basic culture medium and experiment medium were prepared,and 100 mL of Fructus Lycii was diluted 1 time,2 times and 3 times respectively. Eight hours of unmated Drosophila melangaster were collected,followed by ether anesthesia,and Drosophila melangaster were randomly divided into different groups,each group with 20 males and 20 females,then they was cultureed in 2 medium respectively. That's 4 tubes per concentration. Drosophila melangaster were cultureed in(20 ±1) ℃ constant temperature incubator andthe medium was changed every 3 days. Life span,sexual vitality and flyability were measured,the anti-aging effect of Fructus Lycii was evaluated. Results:Drosophila melanogaster was significantly raised the average life expectancy in(male increased by 25.1%,female increased by 27.5%,P<0.01),and the highest life expectancy(male fruit flies increased by 27.3%,female flies increased by 31.7%,P<0.01) in 3 times dose group. The sexual vitality of Drosophila melanogaster increased significantly by 13.5%(P <0.01). Conclusion:The results showed that Fructus Lycii had a significant anti-aging effect on Drosophila melangaster within a certain concentration range.
Objective:To study the anti-aging effect of Fructus Lyciion on Drosophila melangaster. Methods:The basic culture medium and experiment medium were prepared,and 100 mL of Fructus Lycii was diluted 1 time,2 times and 3 times respectively. Eight hours of unmated Drosophila melangaster were collected,followed by ether anesthesia,and Drosophila melangaster were randomly divided into different groups,each group with 20 males and 20 females,then they was cultureed in 2 medium respectively. That's 4 tubes per concentration. Drosophila melangaster were cultureed in(20 ±1) ℃ constant temperature incubator andthe medium was changed every 3 days. Life span,sexual vitality and flyability were measured,the anti-aging effect of Fructus Lycii was evaluated. Results:Drosophila melanogaster was significantly raised the average life expectancy in(male increased by 25.1%,female increased by 27.5%,P<0.01),and the highest life expectancy(male fruit flies increased by 27.3%,female flies increased by 31.7%,P<0.01) in 3 times dose group. The sexual vitality of Drosophila melanogaster increased significantly by 13.5%(P <0.01). Conclusion:The results showed that Fructus Lycii had a significant anti-aging effect on Drosophila melangaster within a certain concentration range.
引文
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[2]龚涛,王晓辉,赵靓,等.枸杞多糖抗氧化作用的研究[J].生物技术,2010,20(1):615-618.
[3]梁国恩,张陈威.枸杞多糖延缓小鼠皮肤衰老的实验研究[J].中国美容医学,2007,16(6):734-736.
[4]罗俊容,刘诗雨,佘赛澜,等.枸杞多糖延缓细胞衰老作用研究[J].亚太传统医药,2017,13(06):3-4.
[5]刘帅,杨小兰,张晓云.长山药粗多糖对果蝇抗衰老作用的研究[J].食品工业科技,2013,34(14):339-341.
[6]陈晓军,张颖,李燕婧,等.山药口服液抗衰老作用的实验研究[J].云南中医中药杂志,2011,32(11):74-75.
[7]阿娃汉,陈瑛,陶大勇.黑果枸杞色素对果蝇性活力及寿命影响的研究[J].塔里木大学学报,2007,19(3):19-20.
[8]周惠萍,陈琼华,王淑如.黑木耳多糖和银耳多糖的抗衰老作用[J].中国药科大学学报,1989,20(5):303-306.
[9]王塔娜,邰丽华,图雅嘎日拉,等.节旋藻藻蛋白对果蝇性活力及繁殖能力的影响[J].内蒙古师范大学学报,2010,39(6):608-611.
[10]杨莉,梁刚,林秀月,等.西番茄莲果汁抗衰老作用的实验研究[J].营养学报,1995,17(4):438.