乙醇摄入对高脂饮食大鼠血清氧化修饰低密度脂蛋白的影响
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摘要
目的探讨乙醇对高脂饮食SD大鼠体内氧化修饰低密度脂蛋白(ox-LDL)的影响。方法选取6周龄雄性SD大鼠16只均喂食高脂饲料,随机分成对照组、乙醇组[50%乙醇,1 ml/(100 g·d)]。于实验前及8周后测定大鼠总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)以及ox-LDL。结果两组实验前后的TC、TG、HDL、LDL、ox-LDL均有明显变化(P<0.05),乙醇组8周后的ox-LDL水平较对照组明显升高(P<0.05),两组的TC、TG、HDL、LDL差异无统计学意义(P>0.05)。结论摄入乙醇可使SD大鼠血清中ox-LDL明显升高。
Objective To investigate the influence of Ethanol intake on serum oxidized low-density lipoprotein in rats fed with high fat diet.Methods 16 male SD rats with 6 weeks of age were fed with high fat diet,and these 16 rats were randomly divided into control group and Ethanol group(50% Ethanol,1 ml/(100 g·d).The total cholesterol(TC),triglyceride(TG),high-density lipoprotein(HDL),low-density lipoprotein(LDL) and oxidized low-density lipoprotein(ox-LDL)levels of blood serum were determined before experiment and after 8 weeks experiment.Results The TC,TG,HDL,LDL and ox-LDL had a significant change in both two group before and after experiment(P<0.05).Compared with control group,the ox-LDL levelof blood serum was obviously increased in ethanol group(P<0.05),but TC,TG,HDL and LDL had no significant difference in both groups(P >0.05).Conclusion Intake of ethanol can significantly increase ox-LDL in serum of SD rats.
Objective To investigate the influence of Ethanol intake on serum oxidized low-density lipoprotein in rats fed with high fat diet.Methods 16 male SD rats with 6 weeks of age were fed with high fat diet,and these 16 rats were randomly divided into control group and Ethanol group(50% Ethanol,1 ml/(100 g·d).The total cholesterol(TC),triglyceride(TG),high-density lipoprotein(HDL),low-density lipoprotein(LDL) and oxidized low-density lipoprotein(ox-LDL)levels of blood serum were determined before experiment and after 8 weeks experiment.Results The TC,TG,HDL,LDL and ox-LDL had a significant change in both two group before and after experiment(P<0.05).Compared with control group,the ox-LDL levelof blood serum was obviously increased in ethanol group(P<0.05),but TC,TG,HDL and LDL had no significant difference in both groups(P >0.05).Conclusion Intake of ethanol can significantly increase ox-LDL in serum of SD rats.
引文
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[19]刘骏,申强.氧化修饰低密度脂蛋白对动脉粥样硬化的影响[J].心血管病防治知识(学术版),2016,(8):148-151.
[20]Steen DL,O′Donoghue ML.Lp-PLA2inhibitors for the reduction of cardiovascular events[J].Cardiol Ther,2013,2(2):125-134.
[21]李铁威,丛祥凤,陈曦.心血管疾病生物标志物——脂蛋白相关磷脂酶A2的临床应用价值[J].中国循环杂志,2016,31(4):410-413.
[22]Maiolino G,Rossitto G,Caielli P,et al.The role of oxidized low-density lipoproteins in atherosclerosis:the myths and the facts[J].Mediators Inflamm,2013,2013:714653.
[2]Makita S,Onoda T,Ohsawa M,et al.Influence of mild-tomoderate alcohol consumption on cardiovascular diseases in men from the general population[J].Atherosclerosis,2012,224(1):222-227.
[3]Razvodovsky YE.Alcohol-attributable fraction of ischemic heart disease mortality in Russia[J].ISRN Cardiol,2013,2013:287869.
[4]Zivkovi觬V,Mileti觬B,Nikoli觬S,et al.Sudden cardiac death and acute drunken state—autopsy study[J].Srpski Arhiv Za Celokupno Lekarstvo,2009,138(9-10):590-594.
[5]刘永娟,谭健苗.酒与动脉粥样硬化[J].中国动脉硬化杂志,2007,15(12):961-963.
[6]林武,吴丽萍.高脂饲料致高脂血症大鼠模型的研究[J].现代实用医学,2013,25(2):171-172.
[7]史清海,伏建峰,路西春,等.酒精灌胃大鼠血浆中乙醇浓度变化[J].实用预防医学,2006,13(5):1322-1323.
[8]屈福超,王爱红,孙永乐,等.乙醇对心肌梗死大鼠冠脉血管新生的影响[J].复旦学报(医学版),2014,41(4):522-525.
[9]沈继平.高脂饮食对SD大鼠胰岛β细胞功能、脂代谢及氧化应激状态的影响[D].上海:复旦大学,2012.
[10]张安邦,黄昕,李令根,等.复合方法制备SD大鼠动脉粥样硬化模型[J].中国中西医结合外科杂志,2015,21(3):282-285.
[11]Zima T,FialováL,Mestek O,et al.Oxidative stress,metabolism of ethanol and a lcohol-related diseases[J].J Biomed Sci,2001,8(1):59-70.
[12]Gratton JP,Bernatchez P,Sessa WC.Caveolae and caveolins in the cardiovascular system[J].Circ Res,2004,94(11):1408-1417.
[13]马景德,赵志文,权莹,等.一氧化氮和氧化修饰低密度脂蛋白水平在乙醇中毒时的变化及其临床意义[J].临床军医杂志,2005,33(1):24-26.
[14]Yamauchi M,Nakano H,Maekawa J,et al.Oxidative stress in obstructive sleep apnea[J].Chest,2005,127(5):1674-1679.
[15]Salvayre R,Negre-Salvayre A,CamaréC.Oxidative theory of atherosclerosis and antioxidants[J].Biochimie,2016,125:281-296.
[16]Moohebati M,Kabirirad V,Ghayour-Mobarhan M,et al.Investigation of serum oxidized low-density lipoprotein Ig G levels in patients with angiographically defined coronary artery disease[J].Int J Vasc Med,2014,2014:845960.
[17]Chen C,Khismatullin DB.Oxidized low-density lipoprotein contributes to atherogenesis via co-activation of macrophages and mast cells[J].PLo S one,2015,10(3):e0123088.
[18]She ZG,Chen HZ,Yan Y,et al.The human paraoxonase gene cluster as a target in the treatment of atherosclerosis[J].Antioxid Redox Signal,2012,16(6):597-632.
[19]刘骏,申强.氧化修饰低密度脂蛋白对动脉粥样硬化的影响[J].心血管病防治知识(学术版),2016,(8):148-151.
[20]Steen DL,O′Donoghue ML.Lp-PLA2inhibitors for the reduction of cardiovascular events[J].Cardiol Ther,2013,2(2):125-134.
[21]李铁威,丛祥凤,陈曦.心血管疾病生物标志物——脂蛋白相关磷脂酶A2的临床应用价值[J].中国循环杂志,2016,31(4):410-413.
[22]Maiolino G,Rossitto G,Caielli P,et al.The role of oxidized low-density lipoproteins in atherosclerosis:the myths and the facts[J].Mediators Inflamm,2013,2013:714653.