柔嫩艾美耳球虫2个抗药株的抗药性评估
|
摘要
为检测实验室长期保存的柔嫩艾美耳球虫马杜拉霉素抗药株和地克珠利抗药株的遗传稳定性,采用鸡体试验法,以抗球虫指数、最适抗球虫活性百分率、病变记分减少率和相对卵囊产量4项指标,评价2个抗药株对地克珠利、癸氧喹酯、马杜拉霉素等3种抗球虫药物的抗药性,并以柔嫩艾美耳球虫敏感株为对照。结果显示,柔嫩艾美耳球虫敏感株对3种抗球虫药物均无抗药性,4项指标全部阴性。柔嫩艾美耳球虫马杜拉霉素抗药株对马杜拉霉素为完全耐药,4项指标全部阳性;对地克珠利、癸氧喹酯则表现为无抗药性,4项指标全部阴性。柔嫩艾美耳球虫地克珠利抗药株对地克珠利表现为完全耐药,4项指标全部阳性;对马杜拉霉素表现为中度耐药,2项指标阳性;对癸氧喹酯则表现为无抗药性,4项指标全部阴性。结果表明,在实验室已繁殖保存10余年的柔嫩艾美耳球虫2个抗药虫株对其诱导药物具有明显抗药性,实验室保种繁殖方法可行,虫株遗传稳定性良好,为利用该虫株进行球虫抗药性机制等相关研究奠定了基础。
In order to detect the drug resistance of Eimeria tenella(E. tenella) maduramicin resistant strain, diclazuril resistant strain and drug sensitive strain to three drugs which include diclazuril, decoquinate and maduramicin, the body-testing method was used for evaluating the effect, including anticoccidial index(ACI), reduction of lesion scores(RLS), relative oocyst production(ROP) and percent of optimum anticoccidial activity(POAA). The results revealed that drug sensitive strain was sensitive to the three drugs and all indicators was negative. Maduramicin resistant strain was serious resistance to maduramicin and all indicators was positive, but it was sensitive to diclazuril and decoquinate and all indicators was negative. Diclazuril resistant strain was serious resistance to diclazuril and all indicators was positive, it was also middle resistance to maduramicin and two indicators was positive, but it was sensitive to decoquinate and allindicators was negative. These results show that the two drug-resistant strains of E. tenella that be propagated and kept for more than 10 years in the laboratory have obvious resistance to maduramicin and diclazuril, respectively, which will indicate that the method that E. tenella propagated and saved in the lab is feasible and the drug-resistance of durg-induced strain can be inherited stably, and it will provide an experimental foundation for drug-resistant mechanism research.
In order to detect the drug resistance of Eimeria tenella(E. tenella) maduramicin resistant strain, diclazuril resistant strain and drug sensitive strain to three drugs which include diclazuril, decoquinate and maduramicin, the body-testing method was used for evaluating the effect, including anticoccidial index(ACI), reduction of lesion scores(RLS), relative oocyst production(ROP) and percent of optimum anticoccidial activity(POAA). The results revealed that drug sensitive strain was sensitive to the three drugs and all indicators was negative. Maduramicin resistant strain was serious resistance to maduramicin and all indicators was positive, but it was sensitive to diclazuril and decoquinate and all indicators was negative. Diclazuril resistant strain was serious resistance to diclazuril and all indicators was positive, it was also middle resistance to maduramicin and two indicators was positive, but it was sensitive to decoquinate and allindicators was negative. These results show that the two drug-resistant strains of E. tenella that be propagated and kept for more than 10 years in the laboratory have obvious resistance to maduramicin and diclazuril, respectively, which will indicate that the method that E. tenella propagated and saved in the lab is feasible and the drug-resistance of durg-induced strain can be inherited stably, and it will provide an experimental foundation for drug-resistant mechanism research.
引文
[1]Bhogal B S,Miller G A,Anderson A C,et al.Potential of a recombinant antigen as a prophylactic vaccine for day-old broiler chickens against Eimeria acervulina and Eimeria tenella infections[J].Vet Immunol Immunop,1992,31(S3-4):323-335.
[2]Jenkins M C,O’Brien C N,Fuller L,et al.A rapid method for determining salinomycin and monensin sensitivity in Eimeria tenella[J].Vet Parasitol,2014,206(3-4):153-158.
[3]Chapman H D.Biochemical,genetic and applied aspects of drug resistance in Eimeria parasites of the fowl[J].Avian Pathol,1997,26(2):221-244.
[4]Waletzky E,Neal R,Hable I.A field strain of Eimeria tenella resistant to sulfonamides[J].J Parasitol,1954,40(2):24.
[5]Mc Dougald L R,Fuller L,Solis J.Drug-sensitivity of99 isolates of coccidia from broiler farms[J].Avian Dis,1986,30(4):690-694.
[6]Mc Dougald L R,Da Silva J M L,Solis J,et al.A survey of sensitivity to anticoccidial drugs in 60 isolates of coccidia from broiler chickens in Brazil and Argentina[J].Avian Dis,1987,31(2):287-292.
[7]孔繁瑶,宁长申,殷佩云.15株柔嫩艾美耳球虫Eimeria tenella对五种抗球虫药的抗药性调查研究[J].北京农业大学学报,1994,20(3):302-308.
[8]李安兴,谢明权,蔡建平,等.广东省肉鸡柔嫩艾美耳球虫(E.tenella)抗药性调查[J].中山大学学报(自然科学版),2000,39(6A):138-144.
[9]李佩国.河北省鸡柔嫩艾美耳球虫地方株的耐药性检测[D].长春:吉林大学,2010.
[10]蔡兰,朱顺海,赵其平,等.绍兴市5个鸡场球虫分离株抗药性试验[J].浙江农业学报,2015,27(4):532-536.
[11]甘德培.抗球虫药与球虫的抗药性研究进展[J].中国兽医寄生虫病,1999,7(2):53-56.
[12]韩红玉,赵其平,陈兆国,等.柔嫩艾美耳球虫地克珠利抗药株和马杜拉霉素抗药株的试验室诱导[J].中国兽医学报,2004,24(2):138-140.
[13]黄兵,赵其平,吴薛忠,等.上海地区鸡球虫对6种抗球虫药的抗药程度研究[J].中国兽医寄生虫病,2001,9(2):1-7.
[14]苏子超.临沂地区鸡球虫病流行病学调查及抗药性研究[D].泰安:山东农业大学,2012.
[15]吴金凤,曾明华,王凤,等.安徽3个地区柔嫩艾美耳球虫对6种药物的抗药性[J].中国兽医寄生虫病,2008,16(6):22-25.
[16]吴昌标,郑文金,陈金雄,等.柔嫩艾美耳球虫莆田株抗药性研究[J].经济动物学报,2013,17(1):19-22转26.
[17]Williams R B.Tracing the emergence of drug-resistance in coccidia(Eimeria spp.)of commercial broiler flocks medicated with decoquinate for the first time in the United Kingdom[J].Vet Parasitol,2006,135(1):1-14.
[18]潘虹,林瑞庆,舒丽,等.柔嫩艾美耳球虫癸氧喹酯耐药株的诱导研究[J].中国畜牧兽医,2012,39(9):191-194.
[19]Kawazoe U,Chapman H D,Shaw M.Sensitivity of field isolates of Eimeria acervulina to salinomycin,maduramicin,and a mixture of clopidol and methyl benzoquate in the chicken[J].Avian Pathology,1991,20(3):439-446.
[20]汪明,孔繁瑶,殷佩云,等.10株柔嫩艾美耳球虫对四种药物的抗药性检测[J].中国农业大学学报,1996,1(5):110-114.
[21]于跃.吉林市部分地区鸡球虫病流行病学调查及综合防制技术研究[D].北京:中国农业科学院,2007.
[22]许金俊,陶建平,彭金彪,等.柔嫩艾美耳球虫扬州分离株对8种抗球虫药的抗药性[J].畜牧与兽医,2008,40(1):18-22.
[23]李蕴玉,李佩国,张香斋,等.柔嫩艾美耳球虫石家庄株的生物学特性与抗药性研究[J].中国兽医学报,2012,32(2):223-227.
[24]周飞亚,黄月月,李琳,等.安徽3株柔嫩艾美耳球虫对5种抗球虫药的耐药性检测[J].中国兽医学报,2015,35(10):1614-1621.
[25]Johnson J,Reid W M.Anticoccidial drugs:lesion scoring techniques in battery and floor-pen experiments with chickens[J].Exp Parasitol,1970,28(1):30-36.
[26]Jeffers T K,Bentley E J.Experimental development of monensin resistance in Eimeria meleagrimitis[J].Poul Sci,1980,59(8):1731-1735.
[27]陈兆国,黄兵,赵其平,等.四项球虫抗药性判定指标的准确性评价[J].中国兽医寄生虫病,2001,9(4):38-42.
[28]钟立军.吉林省部分地区鸡球虫病流行病学调查及抗药性研究[D].长春:吉林大学,2005.
[29]张香斋,张艳英,贾青辉,等.秦皇岛株柔嫩艾美耳球虫对7种常用抗球虫药的耐药性[J].西北农林科技大学学报(自然科学版),2008,36(7):14-18.
[30]韦园园.广西部分地区鸡球虫抗药性的研究[D].南宁:广西大学,2012.
[31]王中宝.安徽三个地区柔嫩艾美耳球虫耐药性分析[D].合肥:安徽农业大学,2013.
[32]蒋禄峰,常丽云,张晶,等.河北省部分地区柔嫩艾美耳球虫野外分离株耐药性检测[J].中国动物保健,2015,17(6):78-81.
[2]Jenkins M C,O’Brien C N,Fuller L,et al.A rapid method for determining salinomycin and monensin sensitivity in Eimeria tenella[J].Vet Parasitol,2014,206(3-4):153-158.
[3]Chapman H D.Biochemical,genetic and applied aspects of drug resistance in Eimeria parasites of the fowl[J].Avian Pathol,1997,26(2):221-244.
[4]Waletzky E,Neal R,Hable I.A field strain of Eimeria tenella resistant to sulfonamides[J].J Parasitol,1954,40(2):24.
[5]Mc Dougald L R,Fuller L,Solis J.Drug-sensitivity of99 isolates of coccidia from broiler farms[J].Avian Dis,1986,30(4):690-694.
[6]Mc Dougald L R,Da Silva J M L,Solis J,et al.A survey of sensitivity to anticoccidial drugs in 60 isolates of coccidia from broiler chickens in Brazil and Argentina[J].Avian Dis,1987,31(2):287-292.
[7]孔繁瑶,宁长申,殷佩云.15株柔嫩艾美耳球虫Eimeria tenella对五种抗球虫药的抗药性调查研究[J].北京农业大学学报,1994,20(3):302-308.
[8]李安兴,谢明权,蔡建平,等.广东省肉鸡柔嫩艾美耳球虫(E.tenella)抗药性调查[J].中山大学学报(自然科学版),2000,39(6A):138-144.
[9]李佩国.河北省鸡柔嫩艾美耳球虫地方株的耐药性检测[D].长春:吉林大学,2010.
[10]蔡兰,朱顺海,赵其平,等.绍兴市5个鸡场球虫分离株抗药性试验[J].浙江农业学报,2015,27(4):532-536.
[11]甘德培.抗球虫药与球虫的抗药性研究进展[J].中国兽医寄生虫病,1999,7(2):53-56.
[12]韩红玉,赵其平,陈兆国,等.柔嫩艾美耳球虫地克珠利抗药株和马杜拉霉素抗药株的试验室诱导[J].中国兽医学报,2004,24(2):138-140.
[13]黄兵,赵其平,吴薛忠,等.上海地区鸡球虫对6种抗球虫药的抗药程度研究[J].中国兽医寄生虫病,2001,9(2):1-7.
[14]苏子超.临沂地区鸡球虫病流行病学调查及抗药性研究[D].泰安:山东农业大学,2012.
[15]吴金凤,曾明华,王凤,等.安徽3个地区柔嫩艾美耳球虫对6种药物的抗药性[J].中国兽医寄生虫病,2008,16(6):22-25.
[16]吴昌标,郑文金,陈金雄,等.柔嫩艾美耳球虫莆田株抗药性研究[J].经济动物学报,2013,17(1):19-22转26.
[17]Williams R B.Tracing the emergence of drug-resistance in coccidia(Eimeria spp.)of commercial broiler flocks medicated with decoquinate for the first time in the United Kingdom[J].Vet Parasitol,2006,135(1):1-14.
[18]潘虹,林瑞庆,舒丽,等.柔嫩艾美耳球虫癸氧喹酯耐药株的诱导研究[J].中国畜牧兽医,2012,39(9):191-194.
[19]Kawazoe U,Chapman H D,Shaw M.Sensitivity of field isolates of Eimeria acervulina to salinomycin,maduramicin,and a mixture of clopidol and methyl benzoquate in the chicken[J].Avian Pathology,1991,20(3):439-446.
[20]汪明,孔繁瑶,殷佩云,等.10株柔嫩艾美耳球虫对四种药物的抗药性检测[J].中国农业大学学报,1996,1(5):110-114.
[21]于跃.吉林市部分地区鸡球虫病流行病学调查及综合防制技术研究[D].北京:中国农业科学院,2007.
[22]许金俊,陶建平,彭金彪,等.柔嫩艾美耳球虫扬州分离株对8种抗球虫药的抗药性[J].畜牧与兽医,2008,40(1):18-22.
[23]李蕴玉,李佩国,张香斋,等.柔嫩艾美耳球虫石家庄株的生物学特性与抗药性研究[J].中国兽医学报,2012,32(2):223-227.
[24]周飞亚,黄月月,李琳,等.安徽3株柔嫩艾美耳球虫对5种抗球虫药的耐药性检测[J].中国兽医学报,2015,35(10):1614-1621.
[25]Johnson J,Reid W M.Anticoccidial drugs:lesion scoring techniques in battery and floor-pen experiments with chickens[J].Exp Parasitol,1970,28(1):30-36.
[26]Jeffers T K,Bentley E J.Experimental development of monensin resistance in Eimeria meleagrimitis[J].Poul Sci,1980,59(8):1731-1735.
[27]陈兆国,黄兵,赵其平,等.四项球虫抗药性判定指标的准确性评价[J].中国兽医寄生虫病,2001,9(4):38-42.
[28]钟立军.吉林省部分地区鸡球虫病流行病学调查及抗药性研究[D].长春:吉林大学,2005.
[29]张香斋,张艳英,贾青辉,等.秦皇岛株柔嫩艾美耳球虫对7种常用抗球虫药的耐药性[J].西北农林科技大学学报(自然科学版),2008,36(7):14-18.
[30]韦园园.广西部分地区鸡球虫抗药性的研究[D].南宁:广西大学,2012.
[31]王中宝.安徽三个地区柔嫩艾美耳球虫耐药性分析[D].合肥:安徽农业大学,2013.
[32]蒋禄峰,常丽云,张晶,等.河北省部分地区柔嫩艾美耳球虫野外分离株耐药性检测[J].中国动物保健,2015,17(6):78-81.