摘要
为检测实验室长期保存的柔嫩艾美耳球虫马杜拉霉素抗药株和地克珠利抗药株的遗传稳定性,采用鸡体试验法,以抗球虫指数、最适抗球虫活性百分率、病变记分减少率和相对卵囊产量4项指标,评价2个抗药株对地克珠利、癸氧喹酯、马杜拉霉素等3种抗球虫药物的抗药性,并以柔嫩艾美耳球虫敏感株为对照。结果显示,柔嫩艾美耳球虫敏感株对3种抗球虫药物均无抗药性,4项指标全部阴性。柔嫩艾美耳球虫马杜拉霉素抗药株对马杜拉霉素为完全耐药,4项指标全部阳性;对地克珠利、癸氧喹酯则表现为无抗药性,4项指标全部阴性。柔嫩艾美耳球虫地克珠利抗药株对地克珠利表现为完全耐药,4项指标全部阳性;对马杜拉霉素表现为中度耐药,2项指标阳性;对癸氧喹酯则表现为无抗药性,4项指标全部阴性。结果表明,在实验室已繁殖保存10余年的柔嫩艾美耳球虫2个抗药虫株对其诱导药物具有明显抗药性,实验室保种繁殖方法可行,虫株遗传稳定性良好,为利用该虫株进行球虫抗药性机制等相关研究奠定了基础。
In order to detect the drug resistance of Eimeria tenella(E. tenella) maduramicin resistant strain, diclazuril resistant strain and drug sensitive strain to three drugs which include diclazuril, decoquinate and maduramicin, the body-testing method was used for evaluating the effect, including anticoccidial index(ACI), reduction of lesion scores(RLS), relative oocyst production(ROP) and percent of optimum anticoccidial activity(POAA). The results revealed that drug sensitive strain was sensitive to the three drugs and all indicators was negative. Maduramicin resistant strain was serious resistance to maduramicin and all indicators was positive, but it was sensitive to diclazuril and decoquinate and all indicators was negative. Diclazuril resistant strain was serious resistance to diclazuril and all indicators was positive, it was also middle resistance to maduramicin and two indicators was positive, but it was sensitive to decoquinate and allindicators was negative. These results show that the two drug-resistant strains of E. tenella that be propagated and kept for more than 10 years in the laboratory have obvious resistance to maduramicin and diclazuril, respectively, which will indicate that the method that E. tenella propagated and saved in the lab is feasible and the drug-resistance of durg-induced strain can be inherited stably, and it will provide an experimental foundation for drug-resistant mechanism research.
引文
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