绍兴市5个鸡场球虫分离株抗药性试验
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摘要
为了解浙江绍兴地区规模化养鸡场防治球虫病的药物使用情况及鸡场球虫对常规药物的抗药性程度,对绍兴市5个县市规模化鸡场采集、分离、繁殖的球虫混合株进行抗马杜拉霉素和抗地克珠利的药物抗药性试验。试验设置了4个组:即感染给马杜拉霉素组、感染给地克珠利组、感染不给药组、健康组;以ACI,POAA,RLS,ROP这4项指标作为球虫抗药性的综合判定标准。结果显示,绍兴市随机抽取的5个规模化鸡场的球虫对这两种药物均产生了完全抗药性。说明绍兴市其余鸡场球虫可能也对马杜拉霉素和地克珠利产生了抗药性。因此,需要养殖户引起足够的重视,以免造成大的经济损失。
Eimeria species were collected from five chicken farms in Shaoxing City,Zhejiang Province,and two anticoccidial drugs,i. e. Maduramicin and Diclazuril,were selected to test their drug resistance. The treatments were set as follows: Group 1 was infected with Eimeria oocysts and was medicated by Maduramicin; group 2 was infected with Eimeria oocysts and was medicated by Diclazuril; group 3 was infected with Eimeria oocysts yet was unmedicated; group 4 was uninfected and unmedicated. Drug resistance was assessed by ACI,POAA,RLS and ROP. The results revealed that all of the Eimeria species isolated were completely resistant to both Maduramicin and Diclazuril. It was suggested that the situation of drug resistance in chicken farms in Shaoxing City was serious. In order to avoid more economic loss,effective methods should be considered to control coccidiosis in this area.
Eimeria species were collected from five chicken farms in Shaoxing City,Zhejiang Province,and two anticoccidial drugs,i. e. Maduramicin and Diclazuril,were selected to test their drug resistance. The treatments were set as follows: Group 1 was infected with Eimeria oocysts and was medicated by Maduramicin; group 2 was infected with Eimeria oocysts and was medicated by Diclazuril; group 3 was infected with Eimeria oocysts yet was unmedicated; group 4 was uninfected and unmedicated. Drug resistance was assessed by ACI,POAA,RLS and ROP. The results revealed that all of the Eimeria species isolated were completely resistant to both Maduramicin and Diclazuril. It was suggested that the situation of drug resistance in chicken farms in Shaoxing City was serious. In order to avoid more economic loss,effective methods should be considered to control coccidiosis in this area.
引文
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[3]黄兵,赵其平,吴薛忠,等.柔嫩艾美耳球虫纯种的初步确定和致病性研究[J].上海畜牧兽医通讯,1993,(5):18-20.
[4]孔繁瑶.家畜寄生虫学[M].北京:农业出版社,1981:418-420.
[5]角田清.鸡球虫病[M].陈谊,明如镜,译.上海:上海科学技术文献出版社,1983:87-94.
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[9]赵其平,韩红玉,黄兵,等.鸡球虫广东清远分离株对七种药物抗药性试验[J].中国兽医寄生虫病,2003,11(2):13-17.
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[11]赵其平,黄兵,陈兆国,等.抗球虫药球佳防治鸡艾美耳球虫5个虫种的笼养试验[J].中国兽医杂志,1999,25(3):15-16.
[12]施祖灏,朱良强,卢运站,等.鸡组织中地克珠利和妥曲珠利残留HPLC检测方法的建立[J].中国兽医学报,2009,29(1):79-81转109.
[13]彭新宇,张建腓,魏文康,等.柔嫩艾美耳球虫野外分离株对3种化学合成药物的抗药性[J].中国兽医学报,2000,20(1):48-50.
[14]许金俊,陶建平,彭金彪,等.柔嫩艾美耳球虫扬州分离株对8种抗球虫药的抗药性[J].畜牧与兽医,2008,40(1):18-22.
[15]魏峰,许越,殷丽红,等.长春市肉鸡球虫抗药性调查[J].黑龙江畜牧兽医:科技版,2010,(7):112-113.
[16]陈兆国,黄兵,史天卫,等.球虫抗药性检测方法评述[J].中国兽医寄生虫病,2001,9(3):47-51.
[17]孔繁瑶,宁长申,殷佩云.15株柔嫩艾美耳球虫Eimeria tenella对五种抗球虫药的抗药性调查研究[J].北京农业大学学报,1994,20(3):302-308.
[18]甘德培,汪明,龙光宗,等.柔嫩艾美耳球虫对四种抗球虫药的交叉抗药性试验[J].中国兽医寄生虫病,1999,7(1):12-14.
[19]陈兆国,黄兵,赵其平,等.四项球虫抗药性判定指标的准确性评价[J].中国兽医寄生虫病,2001,9(4):38-42.
[20]高艳风,毛雪,陶建平,等.柔嫩艾美耳球虫常州株对5种抗球虫药的抗药性分析[J].中国畜牧兽医,2010,37(11):146-149.
[21]吴昌标,郑文金,陈金雄,等.柔嫩艾美耳球虫莆田株抗药性研究[J].经济动物学报,2013,17(1):19-22转26.
[2]Veterinary MD.Sales of antimicrobial products authorized for use as veterinary medicines,antiprotozoals,antifungals,growth promoters and coccidiostats in the UK in 2004[EB/OL].[2014-06-20].http://www.vmd.gov.uk/Publications/Antibiotic/salesanti04.pdf
[3]黄兵,赵其平,吴薛忠,等.柔嫩艾美耳球虫纯种的初步确定和致病性研究[J].上海畜牧兽医通讯,1993,(5):18-20.
[4]孔繁瑶.家畜寄生虫学[M].北京:农业出版社,1981:418-420.
[5]角田清.鸡球虫病[M].陈谊,明如镜,译.上海:上海科学技术文献出版社,1983:87-94.
[6]Johnson J,Reid WM.Anticoccidial drugs:lesion scoring techniques in battery and floor-pen experiments with chicken[J].Experimental Parasitology,1970,28:30-36.
[7]黄兵,赵其平,吴薛忠,等.上海地区鸡球虫对6种抗球虫药的抗药程度研究[J].中国兽医寄生虫病,2001,9(2):1-7.
[8]伊欣,王晓峰,张波.马杜拉霉素的特点及中毒的防治[J].养殖技术顾问,2011,(9):186.
[9]赵其平,韩红玉,黄兵,等.鸡球虫广东清远分离株对七种药物抗药性试验[J].中国兽医寄生虫病,2003,11(2):13-17.
[10]赵其平,倪玉明,李洋,等.安徽两个鸡场球虫的抗药性试验[J].安徽农业科学,2010,38(21):11142-11143转11124.
[11]赵其平,黄兵,陈兆国,等.抗球虫药球佳防治鸡艾美耳球虫5个虫种的笼养试验[J].中国兽医杂志,1999,25(3):15-16.
[12]施祖灏,朱良强,卢运站,等.鸡组织中地克珠利和妥曲珠利残留HPLC检测方法的建立[J].中国兽医学报,2009,29(1):79-81转109.
[13]彭新宇,张建腓,魏文康,等.柔嫩艾美耳球虫野外分离株对3种化学合成药物的抗药性[J].中国兽医学报,2000,20(1):48-50.
[14]许金俊,陶建平,彭金彪,等.柔嫩艾美耳球虫扬州分离株对8种抗球虫药的抗药性[J].畜牧与兽医,2008,40(1):18-22.
[15]魏峰,许越,殷丽红,等.长春市肉鸡球虫抗药性调查[J].黑龙江畜牧兽医:科技版,2010,(7):112-113.
[16]陈兆国,黄兵,史天卫,等.球虫抗药性检测方法评述[J].中国兽医寄生虫病,2001,9(3):47-51.
[17]孔繁瑶,宁长申,殷佩云.15株柔嫩艾美耳球虫Eimeria tenella对五种抗球虫药的抗药性调查研究[J].北京农业大学学报,1994,20(3):302-308.
[18]甘德培,汪明,龙光宗,等.柔嫩艾美耳球虫对四种抗球虫药的交叉抗药性试验[J].中国兽医寄生虫病,1999,7(1):12-14.
[19]陈兆国,黄兵,赵其平,等.四项球虫抗药性判定指标的准确性评价[J].中国兽医寄生虫病,2001,9(4):38-42.
[20]高艳风,毛雪,陶建平,等.柔嫩艾美耳球虫常州株对5种抗球虫药的抗药性分析[J].中国畜牧兽医,2010,37(11):146-149.
[21]吴昌标,郑文金,陈金雄,等.柔嫩艾美耳球虫莆田株抗药性研究[J].经济动物学报,2013,17(1):19-22转26.