宁夏水稻代表性种质及后代抗稻瘟病基因型分析
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摘要
以96份宁夏水稻代表性种质资源和47份杂交后代为试验材料,采用宁夏稻瘟病菌生理小种人工接种和自然诱发相结合的方法对其进行稻瘟病抗性的大田鉴定和病圃鉴定,评价了每个材料的稻瘟病抗性;同时采用8个主效抗稻瘟病基因的功能标记对上述材料进行抗病基因的分子检测,得到了28个不同的抗稻瘟病基因型,将不同基因型的稻瘟病抗性评价进行了差异显著性分析。结果表明,同时含有Pita、Pib、Pikm、Pikh、Pi9和Pi5共6个抗病基因组成的基因型的水稻材料对稻瘟病的抗性极显著高于其他基因型的抗性。Pita基因单独与Pikm、Pikh、Pi2、Pi5基因聚合时均表现出正向聚合效应,其中Pita与Pikh基因的聚合效应最强。当Pib与Pita+Pikh聚合时,表现出负向聚合效应。这为宁夏地区通过基因聚合方法选育广谱持久抗稻瘟病水稻新品种提供了重要的参考依据。
In this study,ninety-six Typical japonica rice germplasm resources and these 47 hybrid progeny in Ningxia province were tested for rice blast resistance in the fields,by using artificial inoculation and natural infection with physiological races of rice blast pathogen.Moreover,eight functional markers,which associated to the respective rice blast resistance genes,were used for marker-assisted detection of the resistance genes in mentioned materials as well as additional 28 different Genotypes.As observed,the resistance of genotypes containing Pita,Pib,Pikm,Pikh,P9 and Pi5 represented significantly high level of resistance than that of other genotypes.The Pita gene,pyramiding with either of Pikm,Pikh,Pi2 and Pi5 genes,showed increased resistance to rice blast,and notably,stacking of Pita and Pikh genes showed highest resistance.However,Pib and Pita+Pikh in a genotype showed reduced rice blast resistance.Thus,this work provides an important reference in breeding for new rice varieties with broad-spectrum resistance,by pyramiding of rice blast resistance genes in Ningxia region.
In this study,ninety-six Typical japonica rice germplasm resources and these 47 hybrid progeny in Ningxia province were tested for rice blast resistance in the fields,by using artificial inoculation and natural infection with physiological races of rice blast pathogen.Moreover,eight functional markers,which associated to the respective rice blast resistance genes,were used for marker-assisted detection of the resistance genes in mentioned materials as well as additional 28 different Genotypes.As observed,the resistance of genotypes containing Pita,Pib,Pikm,Pikh,P9 and Pi5 represented significantly high level of resistance than that of other genotypes.The Pita gene,pyramiding with either of Pikm,Pikh,Pi2 and Pi5 genes,showed increased resistance to rice blast,and notably,stacking of Pita and Pikh genes showed highest resistance.However,Pib and Pita+Pikh in a genotype showed reduced rice blast resistance.Thus,this work provides an important reference in breeding for new rice varieties with broad-spectrum resistance,by pyramiding of rice blast resistance genes in Ningxia region.
引文
[1]Moytrt R,Jia Y L,Richard D C.Structure,function,and coevolution of rice blast resistance genes.Acta Agronomica Sinica,2012,38(3):381-393
[2]肖丹凤,张佩胜,王玲,黄世文.中国稻瘟病菌种群分布及优势生理小种的研究进展.中国水稻科学,2013,27(3):312-320Xiao D F,Zhang P S,Wang L,Huang S W.Research progress on populations and physiological race distribution of rice blast pathogen(Magnaporthe grisea)in China.Chinese Rice Science,2013,27(3):312-320
[3]Skamnioti P,Gurr S J.Against the grain:safeguarding rice from rice blast disease.Trends in Biotechnology,2009,27(3):141-150
[4]Ahn S W,Mukelar A.Rice blast management under upland conditions.In:Progress in Upland Ric Research.Manila:International Rice Research Institute,1986:363-374
[5]任洋,张国民,马军韬,王永力,张丽艳,邓凌韦.针对水稻稻瘟病防治技术的研究.山西农经,2017(14):54Ren Y,Zhang G M,Ma J T,Wang Y L,Zhang L Y,Deng LW.Research on rice blast control technology.Shanxi Agricultural Economy,2017(14):54
[6]曾凡松,向礼波,杨立军,杨小林,杨金松,喻大昭.251份水稻品种(系)对稻瘟病的抗性鉴定及抗性多样性分析.植物病理学报,2011,41(4):399-410Zeng F S,Xiang L B,Yang L J,Yang X L,Yang J S,Yu DZ.Diversity analysis for resistance of 251 rice(Oryza sativa L.)varieties(lines)to rice blast disease.Acta Phytopathologica Sinica,2011,41(4):399-410
[7]李洪亮,李荣田.稻瘟病抗性基因Pi1和Pi2的聚合及其育种价值分析.北方水稻,2010,40(5):7-12,21Li H L,Li R T.The Polymerization of rice-blast resistance genes pi1 and pi2 and analysis on breeding value.North Rice,2010,40(5):7-12,21
[8]鲁秀梅,张宁,陈劲枫,钱春桃.作物基因聚合育种的研究进展.分子植物育种,2017,15(4):1445-1454Lu X M,Zhang N,Chen J F,Qian C T.The research progress in crops pyramiding breeding.Molecular Plant Breeding,2017,15(4):1445-1454
[9]Chen H L,Chen B T,Zhang D P,Xie Y F,Zhang Q.Pathotypes of Pyriculara grisea in rice fields of central and southern China.Plant Dis,2001,8:843-850
[10]于苗苗,戴正元,潘存红,陈夕军,余玲,张晓祥,李育红,肖宁,龚红兵,盛生兰,潘学彪,张洪熙,李爱宏.广谱稻瘟病抗性基因Pigm和Pi2的抗谱差异及与Pi1的互作效应.作物学报,2013,39(11):1927-1934Yu M M,Dai Z Y,Pan C H,Chen X J,Yu L,Zhang X X,Li YH,Xiao N,Gong H B,Sheng S L,Pan X B,Zhang H X,Li AH.Resistance spectrum difference between two broad-spectrum blast resistance genes,Pigm and Pi2,and their interaction effect on Pi1.Acta Agronomica Sinica,2013,39(11):1927-1934
[11]王丽丽,赵家铭,马作斌,郑文静,马殿荣.辽宁地区水稻资源抗稻瘟病基因的检测分析.植物遗传资源学报,2017,18(2):325-339Wang L L,Zhao J M,Ma Z B,Zheng W J,Ma D R.Identificating and analyzing to rice blast resistant genes in rice germplasm resources of Liaoning province.Journal of Plant Genetic Resources,2017,18(2):325-339
[12]陈羽.扬稻6号背景下不同稻瘟病广谱抗性基因聚合效应研究.扬州:扬州大学,2017Chen Y.Polymerization effect of broad-spectrum resistance genes of Magnaporthe grisea under Yangda 6 background.Yangzhou:Yangzhou University,2017
[13]刘士平,李信,汪朝阳,李香花,何予卿.基因聚合对水稻稻瘟病的抗性影响.分子植物育种,2003,1(1):22-26Liu S P,Li X,Wang Z Y,Li X H,He Y Q.Effects of gene pyramiding on rice blast resistance.Molecular Plant Breeding,2003,1(1):22-26
[14]Tabien R E,Li Z,Paterson A H,Marchetti M A,Stansel J W,Pinson S R M,Park W D.Mapping of four major rice blast resistance genes from'Lemont'and'Teqing'and evaluation of their combinatorial effect for field resistance.Theor etical and Applied Genetics,2000,101:1215
[15]李文强,王源超,郑小波.宁夏稻瘟病菌的交配型与育性.中国水稻科学,2007(6):650-656Li W Q,Wang Y C,Zheng X B.Mating type and fertility of Magnaporthe grisea populations from rice in Ningxia Hui Autonomous Region,China.Chinese Journal of Rice Science,2007(6):650-656
[16]Jia Y,Wang Z,Sing P.Development of dominant rice blast Pita resistance gene markers.Crop Science,2002,4(6):2145-2149
[17]刘洋,徐培洲,张红宇,徐建第,吴发强,吴先军.水稻抗稻瘟病Pib基因的分子标记辅助选择与应用.中国农业科学,2008,41(1):9-14Liu Y,Xu P Z,Zhang H Y,Xu J D,Wu F Q,Wu X J.Markerassisted selection and application of blast resistant gene Pib in rice.Scientia Agricultura Sinica,2008,41(1):9-14
[18]王军,杨杰,朱金燕,范方军,李文奇,王芳权,黄转运,仲维功.稻瘟病抗病基因Pikh功能标记的开发及江苏粳稻品种中Pikh的变异.中国水稻科学,2014,28(2):141-147Wang J,Yang J,Zhu J Y,Fan F J,Li W Q,Wang F Q,Huang Z Y,Zhong W G.Development of a functional marker for rice blast resistance gene Pi-kh and natural variation at Pi-kh locus in japonica rice in Jiangsu Province,China.Chinese Journal of Rice Science,2014,28(2):141-147
[19]张羽,冯志峰,崔明珠,张晓娟.稻瘟病抗性基因Pikm在陕西省稻种资源中的分布状况.分子植物育种,2013,11(3):311-316Zhang Y,Feng Z F,Cui M Z,Zhang X J.Distribution of blast resistant gene Pi-km in rice germplasms in shanxi province of northwest China.Molecular Plant Breeding,2013,11(3):311-316
[20]高利军,高汉亮,颜群,周萌,周维永,张晋,邓国富.4个抗稻瘟病基因分子标记的建立及在水稻亲本中的分布.杂交水稻,2010,25(S1):294-298Gao L J,Gao H L,Yan Q,Zhou M,Zhou W Y,Zhang J,Deng G F.Establishment of markers for four blast genes and marker distribution in rice parents.Hybrid Rice,2010,25(S1):294-298
[21]张羽,冯志峰,张晗,莫丹.陕西省水稻种质资源中Pi9基因的分布状况.四川农业大学学报,2013,31(2):115-121Zhang Y,Feng Z F,Zhang H,Mo D.The distribution of Pi9gene in rice germplasm resources in Shaanxi province.Journal of Sichuan Agricultural Uniersity,2013,31(2):115-121
[22]陈德西,陈学伟,雷财林,马炳田,王玉平,李仕贵.转Pi-d2基因水稻对稻瘟病的抗性分析.中国水稻科学,2010,24(1):31-35Chen D X,Chen X W,Lei C L,Ma B T,Wang Y P,Li SG.Rice blast resistance of transgenic rice plants with Pi-d2 gene.Chinese Journal Rice Science,2010,24(1):31-35
[23]林琳.稻瘟病抗性基因的多基因聚合研究.成都:四川农业大学,2010Lin L.The research on multi-gene pyramiding of rice blast resistance.Chengdu:Sichuan Agricultural University,2010
[24]王忠华,贾育林,Robert G.Fjellstrom.水稻抗稻瘟病基因Pi-ta与Pi-ta-2之间的关系.浙江万里学院学报,2004(2):94-97Wang Z H,Jia Y L,Robert G.Fjellstrom.Relationship between rice blast resistance gene Pi-ta and Pi-ta-2.Journal of Zhejiang Wanli University,2004(2):94-97
[2]肖丹凤,张佩胜,王玲,黄世文.中国稻瘟病菌种群分布及优势生理小种的研究进展.中国水稻科学,2013,27(3):312-320Xiao D F,Zhang P S,Wang L,Huang S W.Research progress on populations and physiological race distribution of rice blast pathogen(Magnaporthe grisea)in China.Chinese Rice Science,2013,27(3):312-320
[3]Skamnioti P,Gurr S J.Against the grain:safeguarding rice from rice blast disease.Trends in Biotechnology,2009,27(3):141-150
[4]Ahn S W,Mukelar A.Rice blast management under upland conditions.In:Progress in Upland Ric Research.Manila:International Rice Research Institute,1986:363-374
[5]任洋,张国民,马军韬,王永力,张丽艳,邓凌韦.针对水稻稻瘟病防治技术的研究.山西农经,2017(14):54Ren Y,Zhang G M,Ma J T,Wang Y L,Zhang L Y,Deng LW.Research on rice blast control technology.Shanxi Agricultural Economy,2017(14):54
[6]曾凡松,向礼波,杨立军,杨小林,杨金松,喻大昭.251份水稻品种(系)对稻瘟病的抗性鉴定及抗性多样性分析.植物病理学报,2011,41(4):399-410Zeng F S,Xiang L B,Yang L J,Yang X L,Yang J S,Yu DZ.Diversity analysis for resistance of 251 rice(Oryza sativa L.)varieties(lines)to rice blast disease.Acta Phytopathologica Sinica,2011,41(4):399-410
[7]李洪亮,李荣田.稻瘟病抗性基因Pi1和Pi2的聚合及其育种价值分析.北方水稻,2010,40(5):7-12,21Li H L,Li R T.The Polymerization of rice-blast resistance genes pi1 and pi2 and analysis on breeding value.North Rice,2010,40(5):7-12,21
[8]鲁秀梅,张宁,陈劲枫,钱春桃.作物基因聚合育种的研究进展.分子植物育种,2017,15(4):1445-1454Lu X M,Zhang N,Chen J F,Qian C T.The research progress in crops pyramiding breeding.Molecular Plant Breeding,2017,15(4):1445-1454
[9]Chen H L,Chen B T,Zhang D P,Xie Y F,Zhang Q.Pathotypes of Pyriculara grisea in rice fields of central and southern China.Plant Dis,2001,8:843-850
[10]于苗苗,戴正元,潘存红,陈夕军,余玲,张晓祥,李育红,肖宁,龚红兵,盛生兰,潘学彪,张洪熙,李爱宏.广谱稻瘟病抗性基因Pigm和Pi2的抗谱差异及与Pi1的互作效应.作物学报,2013,39(11):1927-1934Yu M M,Dai Z Y,Pan C H,Chen X J,Yu L,Zhang X X,Li YH,Xiao N,Gong H B,Sheng S L,Pan X B,Zhang H X,Li AH.Resistance spectrum difference between two broad-spectrum blast resistance genes,Pigm and Pi2,and their interaction effect on Pi1.Acta Agronomica Sinica,2013,39(11):1927-1934
[11]王丽丽,赵家铭,马作斌,郑文静,马殿荣.辽宁地区水稻资源抗稻瘟病基因的检测分析.植物遗传资源学报,2017,18(2):325-339Wang L L,Zhao J M,Ma Z B,Zheng W J,Ma D R.Identificating and analyzing to rice blast resistant genes in rice germplasm resources of Liaoning province.Journal of Plant Genetic Resources,2017,18(2):325-339
[12]陈羽.扬稻6号背景下不同稻瘟病广谱抗性基因聚合效应研究.扬州:扬州大学,2017Chen Y.Polymerization effect of broad-spectrum resistance genes of Magnaporthe grisea under Yangda 6 background.Yangzhou:Yangzhou University,2017
[13]刘士平,李信,汪朝阳,李香花,何予卿.基因聚合对水稻稻瘟病的抗性影响.分子植物育种,2003,1(1):22-26Liu S P,Li X,Wang Z Y,Li X H,He Y Q.Effects of gene pyramiding on rice blast resistance.Molecular Plant Breeding,2003,1(1):22-26
[14]Tabien R E,Li Z,Paterson A H,Marchetti M A,Stansel J W,Pinson S R M,Park W D.Mapping of four major rice blast resistance genes from'Lemont'and'Teqing'and evaluation of their combinatorial effect for field resistance.Theor etical and Applied Genetics,2000,101:1215
[15]李文强,王源超,郑小波.宁夏稻瘟病菌的交配型与育性.中国水稻科学,2007(6):650-656Li W Q,Wang Y C,Zheng X B.Mating type and fertility of Magnaporthe grisea populations from rice in Ningxia Hui Autonomous Region,China.Chinese Journal of Rice Science,2007(6):650-656
[16]Jia Y,Wang Z,Sing P.Development of dominant rice blast Pita resistance gene markers.Crop Science,2002,4(6):2145-2149
[17]刘洋,徐培洲,张红宇,徐建第,吴发强,吴先军.水稻抗稻瘟病Pib基因的分子标记辅助选择与应用.中国农业科学,2008,41(1):9-14Liu Y,Xu P Z,Zhang H Y,Xu J D,Wu F Q,Wu X J.Markerassisted selection and application of blast resistant gene Pib in rice.Scientia Agricultura Sinica,2008,41(1):9-14
[18]王军,杨杰,朱金燕,范方军,李文奇,王芳权,黄转运,仲维功.稻瘟病抗病基因Pikh功能标记的开发及江苏粳稻品种中Pikh的变异.中国水稻科学,2014,28(2):141-147Wang J,Yang J,Zhu J Y,Fan F J,Li W Q,Wang F Q,Huang Z Y,Zhong W G.Development of a functional marker for rice blast resistance gene Pi-kh and natural variation at Pi-kh locus in japonica rice in Jiangsu Province,China.Chinese Journal of Rice Science,2014,28(2):141-147
[19]张羽,冯志峰,崔明珠,张晓娟.稻瘟病抗性基因Pikm在陕西省稻种资源中的分布状况.分子植物育种,2013,11(3):311-316Zhang Y,Feng Z F,Cui M Z,Zhang X J.Distribution of blast resistant gene Pi-km in rice germplasms in shanxi province of northwest China.Molecular Plant Breeding,2013,11(3):311-316
[20]高利军,高汉亮,颜群,周萌,周维永,张晋,邓国富.4个抗稻瘟病基因分子标记的建立及在水稻亲本中的分布.杂交水稻,2010,25(S1):294-298Gao L J,Gao H L,Yan Q,Zhou M,Zhou W Y,Zhang J,Deng G F.Establishment of markers for four blast genes and marker distribution in rice parents.Hybrid Rice,2010,25(S1):294-298
[21]张羽,冯志峰,张晗,莫丹.陕西省水稻种质资源中Pi9基因的分布状况.四川农业大学学报,2013,31(2):115-121Zhang Y,Feng Z F,Zhang H,Mo D.The distribution of Pi9gene in rice germplasm resources in Shaanxi province.Journal of Sichuan Agricultural Uniersity,2013,31(2):115-121
[22]陈德西,陈学伟,雷财林,马炳田,王玉平,李仕贵.转Pi-d2基因水稻对稻瘟病的抗性分析.中国水稻科学,2010,24(1):31-35Chen D X,Chen X W,Lei C L,Ma B T,Wang Y P,Li SG.Rice blast resistance of transgenic rice plants with Pi-d2 gene.Chinese Journal Rice Science,2010,24(1):31-35
[23]林琳.稻瘟病抗性基因的多基因聚合研究.成都:四川农业大学,2010Lin L.The research on multi-gene pyramiding of rice blast resistance.Chengdu:Sichuan Agricultural University,2010
[24]王忠华,贾育林,Robert G.Fjellstrom.水稻抗稻瘟病基因Pi-ta与Pi-ta-2之间的关系.浙江万里学院学报,2004(2):94-97Wang Z H,Jia Y L,Robert G.Fjellstrom.Relationship between rice blast resistance gene Pi-ta and Pi-ta-2.Journal of Zhejiang Wanli University,2004(2):94-97