灯盏花农艺性状与经济性状相关性分析
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摘要
为深入了解灯盏花主要农艺性状和经济性状间的相关性,高效地选育新品种,本试验在田间选择了100株灯盏花植株,测定了主要农艺性状与活性成分含量,并进行了相关性分析。研究发现灯盏花单株间叶片数、分支数、干重和活性成分含量均差异巨大,叶片数、分支数、单株干重、活性成分含量均相差10倍以上,灯盏乙素最高含量为3.38%,最低仅有0.11%。相关分析表明,灯盏花单株干重与叶片数和分支数显著正相关,两类成分(灯盏乙素和咖啡酰奎宁酸酯类)含量也为正相关。同时,尽管叶片数、分支数和干重与活性成分含量负相关,但是与经济产量成正相关。通过研究发现,灯盏花单株产量主要由叶片数和分支数决定,灯盏乙素含量与咖啡酰奎宁酸酯类的含量正相关。因此,育种中可以选择多分支、多叶片和高灯盏乙素含量的单株建立群体,培育出高产、高经济产量的专用型新品种。
In order to understand the correlation between the main agronomic traits and economic characters of Erigeron breviscapus and make new variety breeding efficiently, 100 plants of Erigeron breviscapus in the field were selected to measure the main agronomic traits and active component content, and then the correlation analysis was carried out. The results showed that there was a significant difference in the number of leaves,branches, dry weight and active component content of per plant. The differences of leaf number, branch number,dry weight of per plant and active component content were more than 10 times. The highest content of scutellarin was 3.38%, while the lowest was 0.11%. The correlation analysis showed that the dry weight of per Erigeron breviscapus was positively correlated with leaf number and branch number, and the contents of two components(scutellarin and caffeic acid quinic acid esters) were also positively correlated. At the same time, although the number of leaves, the number of branches and the dry weight were negatively correlated with the content of active components, they were positively related to the economic yield. The study found that yield per plant of Erigeron breviscapus was mainly determined by the number of leaves and branches, and the content of scutellarin and caffeoylquinic acid esters were positively correlated. Therefore, in breeding, multi branch, multi leaf plant with high content of scutellarin could be selected to establish groups and breed new special varieties for high yield and economy yield.
In order to understand the correlation between the main agronomic traits and economic characters of Erigeron breviscapus and make new variety breeding efficiently, 100 plants of Erigeron breviscapus in the field were selected to measure the main agronomic traits and active component content, and then the correlation analysis was carried out. The results showed that there was a significant difference in the number of leaves,branches, dry weight and active component content of per plant. The differences of leaf number, branch number,dry weight of per plant and active component content were more than 10 times. The highest content of scutellarin was 3.38%, while the lowest was 0.11%. The correlation analysis showed that the dry weight of per Erigeron breviscapus was positively correlated with leaf number and branch number, and the contents of two components(scutellarin and caffeic acid quinic acid esters) were also positively correlated. At the same time, although the number of leaves, the number of branches and the dry weight were negatively correlated with the content of active components, they were positively related to the economic yield. The study found that yield per plant of Erigeron breviscapus was mainly determined by the number of leaves and branches, and the content of scutellarin and caffeoylquinic acid esters were positively correlated. Therefore, in breeding, multi branch, multi leaf plant with high content of scutellarin could be selected to establish groups and breed new special varieties for high yield and economy yield.
引文
Dong Y.,Zhang Y.H.,Liu Y.Y.,Li Z.Q.,and Zhang W.J.,2010,Simultaneous determination of four effective phenolic acids in herba erigerontis by HPLC,Zhongguo Yiyao Gongye Zazhi(Chinese Journal of Pharmaceuticals),41(6):447-449(董媛,张赟华,刘莹莹,李忠琼,张雯洁,2010,灯盏细辛中4种酚酸类有效成分的HPLC法测定,中国医药工业杂志,41(6):447-449)
Guo C.,Zhu Y.,Weng Y.,Wang S.,Guan Y.,Wei G.,Yin Y.,Xi M.,and Wen A.,2014,Therapeutic time window and underlying therapeutic mechanism of breviscapine injection against cerebral ischemia/reperfusion injury in rats,J.Ethnopharmacol.,151(1):660-666
Han Y.Y.,Ma Y.Q.,Li D.Z.,and Xu Z.Q.,2015,The influences of Arabidopsis Pri-miR156a on flowering time of Nicotiana tabacum,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),34(2):350-356(韩瑶瑶,马燕勤,李典珍,徐子勤,2015,拟南芥Pri-miR156a基因对烟草开花时间的影响,基因组学与应用生物学,34(2):350-356)
Huang Z.J.,He S.A.,and Lei B.,2014,Clinical analysis of acute cerebral infarction by dengzhanhua injection and xiongqin injection combined with xuesaitong treatment,Zhongyaocai(Journal of Chinese Medicinal Materials),37(6):1093-1095(黄振坚,何世安,雷斌,2014,灯盏花,芎嗪注射液联合血塞通治疗急性脑梗死的临床分析,中药材,37(6):1093-1095)
Jiang L.,Hu Y.,He X.,Lv Q.,Wang T.H.,and Xia Q.J.,2017a,Breviscapine reduces neuronal injury caused by traumatic brain injury insult:partly associated with suppression of interleukin-6 expression,Neural Regen.Res.,12(1):90-95
Jiang L.,Xia Q.J.,Dong X.J.,Hu Y.,Chen Z.W.,Chen K.,Wang K.H.,Liu J.,and Wang T.H.,2017b,Neuroprotective effect of breviscapine on traumatic brain injury in rats associated with the inhibition of GSK3βsignaling pathway,Brain Res.,1660:1-9
Jiang N.H.,Zhang G.H.,Zhang J.J.,Shu L.P.,Zhang W.,Long G.Q.,Liu T.,Meng Z.G.,Chen J.W.,and Yang S.C.,2014,Analysis of the transcriptome of Erigeron breviscapus uncovers putative scutellarin and chlorogenic acids biosynthetic genes and genetic markers,PLoS One,9(6):e100357
Kalve S.,Vos D.D.,and Beemster G.T.S.,2014,Leaf development:a cellular perspective,Front.Plant Sci.,5(362):362
Li X.B.,Wang R.B.,Shen Y.,Meng Z.G.,Chen J.W.,Yang J.W.,and Yang S.C.,2013,Simultaneous determination of chlorogenic acid,scutellarin,3,5-dicaffeoylquinic acid,4,5-dicaffeoylquinic acid in different parts of Erigeron breviscapus by high-performance liquid chromatography,Zhongguo Zhongyao Zazhi(Chinese Journal of Chinese Materia Medica),38(14):2237-2240(李晓波,汪瑞波,沈勇,孟珍贵,陈军文,杨建文,杨生超,2013,HPLC测定灯盏花不同部位绿原酸,灯盏乙素,3,5-二咖啡酰奎宁酸和4,5-二咖啡酰奎宁酸含量,中国中药杂志,38(14):2237-2240)
Liu X.D.,Yao L.,Sun D.,Zhu X.W.,Liu Q.,Xu T.H.,and Wang L.N.,2016,Effect of breviscapine injection on clinical parameters in diabetic nephropathy:a meta-analysis of randomized controlled trials,Exp.Ther.Med.,12(3):1383-1397
McSteen P.,Laudencia-Chingcuanco D.,and Colasanti J.,2000,A floret by any other name:control of meristem identity in maize,Trends Plant Sci.,5(2):61-66
Sun H.D.,and Zhao Q.S.,2009,A drug for treating cardio-cerebrovascular diseases-phenolic compds of Erigeron breviscapus,Huaxue Jinzhan(Progress in Chemistry),21(1):77-83(孙汉董,赵勤实,2009,防治心脑血管疾病药物---灯盏细辛酚的研究与开发,化学进展,21(1):77-83)
Yang S.C.,Xu S.Z.,Li L.,Wen G.S.,Liu Y.G.,and Xiao F.H.,2008,Phenotypic diversity of populations in germplasm resources of erigeron breviscapus,Xibei Zhiwu Xuebao(Acta Botanica Boreali-Occidentalia Sinica),28(8):1573-1579(杨生超,徐绍忠,李黎,文国松,刘有贵,萧凤回,2008,灯盏花种质资源群体表型多样性研究,西北植物学报,28(8):1573-1579)
Yang S.C.,Yang J.W.,Pan Y.H.,Li X.G.,Liu B.H.,Zhang Q.,Wen G.S.,and Wang P.L.,2010,Comparison on agronomy and quality characters and breeding of new strains of Erigeron breviscapus,Zhongguo Zhongyao Zazhi(Chinese Journal of Chinese Materia Medica),35(5):554-557(杨生超,杨建文,潘应花,李兴国,刘炳华,张琼,文国松,王平理,2010,灯盏花新品系选育及农艺与品质性状比较,中国中药杂志,35(5):554-557)
Yang X.F.,He W.,Lu W.H.,and Zeng F.D.,2003,Effects of scutellarin on liver function after brain ischemia/reperfusion in rats,Acta Pharmacol.Sin.,24(11):1118-1124
Yu M.D.,Zhang S.X.,Zhao Y.,Song W.L.,Zhang G.J.,Liu G.Z.,Yang S.C.,and Zhang G.H.,2016,Cloning and expression analysis of key enzyme gene in Scutellarin Biosy-nthesis,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),14(22):1133-1139(喻孟冬,张石先,赵艳,宋婉玲,张高菊,刘冠泽,杨生超,张广辉,2016,灯盏乙素生物合成关键酶基因的克隆与表达分析,分子植物育种,14(22):1133-1139)
Yue F.W.,Li M.H.,Ya N.L.,Xi P.P.,and Gui X.P.,2010,Arapid method for qualitative and quantitative analysis of major constituents in Dengzhanxixin injection by LC-DAD-ES-I-MSn,Chromatographia,71(9):845-853
Zhang M.Y.,Fan S.J.,Li L.P.,Wu B.Y.,and Wang Y.,2011,The anti-injury effect of breviscapine injection on the hypoxic ischemic brain damage of neonatal rats and the expression of Bcl-2 and Bax,Zhongguo Yingyong Shenglixue Zazhi(Chinese Journal of Applied Physiology),27(2):196-200(张明艳,范淑娟,李利平,武变瑛,王宇,2011,灯盏花注射液抗新生鼠缺氧缺血性脑损伤的作用及对Bcl-2,Bax蛋白表达的影响,中国应用生理学杂志,27(2):196-200)
Zhang P.Y.,Guo T.,He H.Y.,Yang L.Q.,and Deng Y.H.,2017,Breviscapine confers a neuroprotective efficacy against transient focal cerebral ischemia by attenuating neuronal and astrocytic autophagy in the penumbra,Biomed.Pharmacother.,90:69-76
Zhang W.,Wei X.,Meng H.L.,Ma C.H.,Jiang N.H.,Zhang G.H.,and Yang S.C.,2015,Transcriptomic comparison of the self-pollinated and cross-pollinated flowers of Erigeron breviscapus to analyze candidate self-incompatibility-associated genes,BMC Plant Biol.,15:248
Guo C.,Zhu Y.,Weng Y.,Wang S.,Guan Y.,Wei G.,Yin Y.,Xi M.,and Wen A.,2014,Therapeutic time window and underlying therapeutic mechanism of breviscapine injection against cerebral ischemia/reperfusion injury in rats,J.Ethnopharmacol.,151(1):660-666
Han Y.Y.,Ma Y.Q.,Li D.Z.,and Xu Z.Q.,2015,The influences of Arabidopsis Pri-miR156a on flowering time of Nicotiana tabacum,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),34(2):350-356(韩瑶瑶,马燕勤,李典珍,徐子勤,2015,拟南芥Pri-miR156a基因对烟草开花时间的影响,基因组学与应用生物学,34(2):350-356)
Huang Z.J.,He S.A.,and Lei B.,2014,Clinical analysis of acute cerebral infarction by dengzhanhua injection and xiongqin injection combined with xuesaitong treatment,Zhongyaocai(Journal of Chinese Medicinal Materials),37(6):1093-1095(黄振坚,何世安,雷斌,2014,灯盏花,芎嗪注射液联合血塞通治疗急性脑梗死的临床分析,中药材,37(6):1093-1095)
Jiang L.,Hu Y.,He X.,Lv Q.,Wang T.H.,and Xia Q.J.,2017a,Breviscapine reduces neuronal injury caused by traumatic brain injury insult:partly associated with suppression of interleukin-6 expression,Neural Regen.Res.,12(1):90-95
Jiang L.,Xia Q.J.,Dong X.J.,Hu Y.,Chen Z.W.,Chen K.,Wang K.H.,Liu J.,and Wang T.H.,2017b,Neuroprotective effect of breviscapine on traumatic brain injury in rats associated with the inhibition of GSK3βsignaling pathway,Brain Res.,1660:1-9
Jiang N.H.,Zhang G.H.,Zhang J.J.,Shu L.P.,Zhang W.,Long G.Q.,Liu T.,Meng Z.G.,Chen J.W.,and Yang S.C.,2014,Analysis of the transcriptome of Erigeron breviscapus uncovers putative scutellarin and chlorogenic acids biosynthetic genes and genetic markers,PLoS One,9(6):e100357
Kalve S.,Vos D.D.,and Beemster G.T.S.,2014,Leaf development:a cellular perspective,Front.Plant Sci.,5(362):362
Li X.B.,Wang R.B.,Shen Y.,Meng Z.G.,Chen J.W.,Yang J.W.,and Yang S.C.,2013,Simultaneous determination of chlorogenic acid,scutellarin,3,5-dicaffeoylquinic acid,4,5-dicaffeoylquinic acid in different parts of Erigeron breviscapus by high-performance liquid chromatography,Zhongguo Zhongyao Zazhi(Chinese Journal of Chinese Materia Medica),38(14):2237-2240(李晓波,汪瑞波,沈勇,孟珍贵,陈军文,杨建文,杨生超,2013,HPLC测定灯盏花不同部位绿原酸,灯盏乙素,3,5-二咖啡酰奎宁酸和4,5-二咖啡酰奎宁酸含量,中国中药杂志,38(14):2237-2240)
Liu X.D.,Yao L.,Sun D.,Zhu X.W.,Liu Q.,Xu T.H.,and Wang L.N.,2016,Effect of breviscapine injection on clinical parameters in diabetic nephropathy:a meta-analysis of randomized controlled trials,Exp.Ther.Med.,12(3):1383-1397
McSteen P.,Laudencia-Chingcuanco D.,and Colasanti J.,2000,A floret by any other name:control of meristem identity in maize,Trends Plant Sci.,5(2):61-66
Sun H.D.,and Zhao Q.S.,2009,A drug for treating cardio-cerebrovascular diseases-phenolic compds of Erigeron breviscapus,Huaxue Jinzhan(Progress in Chemistry),21(1):77-83(孙汉董,赵勤实,2009,防治心脑血管疾病药物---灯盏细辛酚的研究与开发,化学进展,21(1):77-83)
Yang S.C.,Xu S.Z.,Li L.,Wen G.S.,Liu Y.G.,and Xiao F.H.,2008,Phenotypic diversity of populations in germplasm resources of erigeron breviscapus,Xibei Zhiwu Xuebao(Acta Botanica Boreali-Occidentalia Sinica),28(8):1573-1579(杨生超,徐绍忠,李黎,文国松,刘有贵,萧凤回,2008,灯盏花种质资源群体表型多样性研究,西北植物学报,28(8):1573-1579)
Yang S.C.,Yang J.W.,Pan Y.H.,Li X.G.,Liu B.H.,Zhang Q.,Wen G.S.,and Wang P.L.,2010,Comparison on agronomy and quality characters and breeding of new strains of Erigeron breviscapus,Zhongguo Zhongyao Zazhi(Chinese Journal of Chinese Materia Medica),35(5):554-557(杨生超,杨建文,潘应花,李兴国,刘炳华,张琼,文国松,王平理,2010,灯盏花新品系选育及农艺与品质性状比较,中国中药杂志,35(5):554-557)
Yang X.F.,He W.,Lu W.H.,and Zeng F.D.,2003,Effects of scutellarin on liver function after brain ischemia/reperfusion in rats,Acta Pharmacol.Sin.,24(11):1118-1124
Yu M.D.,Zhang S.X.,Zhao Y.,Song W.L.,Zhang G.J.,Liu G.Z.,Yang S.C.,and Zhang G.H.,2016,Cloning and expression analysis of key enzyme gene in Scutellarin Biosy-nthesis,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),14(22):1133-1139(喻孟冬,张石先,赵艳,宋婉玲,张高菊,刘冠泽,杨生超,张广辉,2016,灯盏乙素生物合成关键酶基因的克隆与表达分析,分子植物育种,14(22):1133-1139)
Yue F.W.,Li M.H.,Ya N.L.,Xi P.P.,and Gui X.P.,2010,Arapid method for qualitative and quantitative analysis of major constituents in Dengzhanxixin injection by LC-DAD-ES-I-MSn,Chromatographia,71(9):845-853
Zhang M.Y.,Fan S.J.,Li L.P.,Wu B.Y.,and Wang Y.,2011,The anti-injury effect of breviscapine injection on the hypoxic ischemic brain damage of neonatal rats and the expression of Bcl-2 and Bax,Zhongguo Yingyong Shenglixue Zazhi(Chinese Journal of Applied Physiology),27(2):196-200(张明艳,范淑娟,李利平,武变瑛,王宇,2011,灯盏花注射液抗新生鼠缺氧缺血性脑损伤的作用及对Bcl-2,Bax蛋白表达的影响,中国应用生理学杂志,27(2):196-200)
Zhang P.Y.,Guo T.,He H.Y.,Yang L.Q.,and Deng Y.H.,2017,Breviscapine confers a neuroprotective efficacy against transient focal cerebral ischemia by attenuating neuronal and astrocytic autophagy in the penumbra,Biomed.Pharmacother.,90:69-76
Zhang W.,Wei X.,Meng H.L.,Ma C.H.,Jiang N.H.,Zhang G.H.,and Yang S.C.,2015,Transcriptomic comparison of the self-pollinated and cross-pollinated flowers of Erigeron breviscapus to analyze candidate self-incompatibility-associated genes,BMC Plant Biol.,15:248