丹凤牡丹花遗传毒理学研究
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摘要
目的通过3项致突变实验,了解丹凤牡丹花的遗传毒性,并对其安全性做出评价。方法骨髓细胞微核试验:通过2次经口灌胃小鼠后观察计数含微核PCE数。Ames试验:利用试验菌株的鼠伤寒沙门氏菌,氨酸缺陷型4株进行试验,最终计数各皿回变菌落数。小鼠精子畸形试验:给予试验样品每日灌胃小鼠,连续5d,末次灌胃后30d计数畸形精子数。结果骨髓细胞微核试验、Ames试验、小鼠精子畸形试验结果均为阴性。结论丹凤牡丹花遗传毒性无毒,安全性较高。
objective To understand the genotoxicity of Danfeng peony flower by 3 mutagenic experiments and evaluate its safety. Methods Micronucleus test of bone marrow cells: The number of micronucleated PCEs was counted by two times of oral gavage mice. Ames test: The test strains of Salmonella typhimurium, four strains of acid-deficient type were used for the test, and the number of colonies of each dish was counted. Mouse sperm abnormality test: Mice were intragastrically administered daily for 5 consecutive days, and the number of abnormal sperm was counted 30 days after the last gavage. Results The results of micronucleus test of bone marrow cells,Ames test and mouse sperm abnormality test in mice were all negative. Conclusion Danfeng peony flower has no genetic toxicity, and has high safety.
objective To understand the genotoxicity of Danfeng peony flower by 3 mutagenic experiments and evaluate its safety. Methods Micronucleus test of bone marrow cells: The number of micronucleated PCEs was counted by two times of oral gavage mice. Ames test: The test strains of Salmonella typhimurium, four strains of acid-deficient type were used for the test, and the number of colonies of each dish was counted. Mouse sperm abnormality test: Mice were intragastrically administered daily for 5 consecutive days, and the number of abnormal sperm was counted 30 days after the last gavage. Results The results of micronucleus test of bone marrow cells,Ames test and mouse sperm abnormality test in mice were all negative. Conclusion Danfeng peony flower has no genetic toxicity, and has high safety.
引文
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[3]菏泽地方史志办.菏泽牡丹志[M].香港:银河出版社,2004.Heze Local History Office.Heze peony chronicles[M].Hongkong:Galaxy Publishing House,2004.
[4]洛阳市志.牡丹志[M].郑州:中州古籍出版社,1998.Luoyang Chronicles.Peony[M].Zhengzhou:Zhongzhou Ancient Book Press,1998.
[5]游玉明,杨帆,熊运海.牡丹花的综合利用与开发前景[J].北方园艺,2011(1):67-69.You YM,Yang F,Xiong YH.Comprehensive utilization and development prospect of peony flower[J].Northern Hortic,2011,(1):67-69.
[6]王新娣,石晓峰,王斌利,等.牡丹化学成分的研究进展[J].中成药,2018,(1):171-176.Wang XD,Shi XF,Wang BL,et al.Progress in the study of the chemical constituents of peony[J].Chin Pat Med,2018,(1):171-176.
[7]詹建国.一种牡丹花茶及其制备方法:中国,200610086920.5[P].2006-11-15.Zhan JG.A kind of peony flower tea and its preparation method:China,200610086920.5[P].2006-11-15.
[8]谢韬,范文今,杨鸿武.牡丹花的亚慢性毒性研究[J].中国卫生检验杂志,2014,24(13):1868-1870.Xie T,Fan WJ,Yang HW.Research on subchronic toxicity of peony flowers[J].Chin J Food Hyg,2014,24(13):1868-1870.
[9]GB 15193.4-2014食品安全性毒理学评价程序和方法[S].GB 15193.4-2014 Procedures and methods for toxicological assessment of food safety[S].
[10]GB 15193.5-2014食品安全性毒理学评价程序和方法[S].GB 15193.5-2014 Procedures and methods for toxicological assessment of food safety[S].
[11]GB 15193.7-2014食品安全性毒理学评价程序和方法[S].GB 15193.7-2014 Procedures and methods for toxicological assessment of food safety[S].
[12]陈炳卿.营养与食品卫生学[M].北京:人民卫生出版社,1995.Cheng BQ.Nutrition and food hygiene[M].Beijing:People's Health Press,1995.
[13]保健食品检验与评价技术规范[M].北京:中华人民共和国卫生部,2014.Health food inspection and evaluation technical specification[M].Beijing:Ministry of health of People's Republic of China,2014.
[14]张鸭关,赵红艳,吴丽芳,等.一朵云不同部位营养成分分析及安全性评价[J].食品研究与开发,2017,38(15):12-16.Zhang YG,Zhao HY,Wu LF,et al.Nutritional components analysis and safety evaluation of different tissues of Botrychium ternatum(Thunb.)Sw.[J].Food Res Dev,2017,38(15):12-16.
[15]王斌利,王新娣,董颖,等.牡丹的食药价值研究进展[J].甘肃医药,2017,36(2):96-98.Wamg BL,Wang XD,Dong Y,et al.Research progress on the value of food and medicine of peony[J].Gansu Med,2017,36(2):96-98.