锌胁迫对黑小麦种子萌发、幼苗生长和酶活性的影响
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摘要
【目的】为黑小麦在锌胁迫下的耐锌生理研究及其耐锌育种提供参考依据。【方法】以4个黑小麦品种(系)为材料,利用锌胁迫的方法,研究0、50、100、200、400、800 mg/L Zn SO4溶液对黑小麦种子萌发、幼苗生长和酶活性的影响。【结果】4个黑小麦品种(系)的苗高、鲜重、发芽率、根冠比、SOD和CAT活性均随着锌浓度的增加,呈现先增加后减少的趋势;根长则呈逐渐减小的趋势;POD活性和须根数随锌浓度升高而升高。【结论】锌浓度在50~100 mg/L可以更好的促进黑小麦生长。
【Objective】To provide basic reference for triticale( black wheat) physiological research on zinc tolerance and breeding under zinc stress.【Method】The seeds were treated with 50,100,200,400 or800 mg Zn / L and then incubated. Seven days after germination the height,root length,weight,and enzyme activities of the seedlings were measured. 【Result】The results indicated that such data as seeding height,fresh weight,germination rate,root- shoot ratio and CAT,SOD firstly rose and then dropped with the increasing of zinc concentration under the zinc stress. The root length dropped and POD,fibrous root number rose with the increasing of zinc concentration under the zinc stress. 【Conclusion】In conclusion,50 to 100 mg Zn / L can promote the growth of triticale( black wheat).
【Objective】To provide basic reference for triticale( black wheat) physiological research on zinc tolerance and breeding under zinc stress.【Method】The seeds were treated with 50,100,200,400 or800 mg Zn / L and then incubated. Seven days after germination the height,root length,weight,and enzyme activities of the seedlings were measured. 【Result】The results indicated that such data as seeding height,fresh weight,germination rate,root- shoot ratio and CAT,SOD firstly rose and then dropped with the increasing of zinc concentration under the zinc stress. The root length dropped and POD,fibrous root number rose with the increasing of zinc concentration under the zinc stress. 【Conclusion】In conclusion,50 to 100 mg Zn / L can promote the growth of triticale( black wheat).
引文
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[14]齐笑笑,肖家欣,徐春丽,等.锌胁迫对3种柑橘砧木的生理特性和锌分配的影响[J].中国农学通报,2009,25(24):313QI Xiao-xiao,XIAO Jia-xin,XU Chun-li,et al.(2009).Effects of zinc stress on physiological characteristics and zinc distribution of three species of Citrus[J].Chinese Agricultural Bulletin,25(24):313-317.(in Chinese)
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[17]宗宪春,宗灿华,王书臻,等.锌胁迫对虞美人种子萌发的影响[J].安徽农业科学,2011,39(33):20 371-20 372.ZONG Xian-chun,ZONG Can-hua,WANG Shu-zhen,et al.(2011).Effects of zinc stress on the germination of poppy seed[J].Anhui Agricultural Sciences,39(33):20,371-20,372.(in Chinese)
[18]常学秀,文传浩,沈其荣,等.锌厂Pb污染农田小麦根际与非根际土壤酶活性特征研究[J].生态学杂志,2001,20(4):5-8.CHANG Xue-xiu,WEN Chuan-hao,SHEN Qi-rong,et al.(2001).Characteristics of soil enzyme activity in the rhizosphere and non-rhizosphere soil of Pb polluted by zinc plant[J].Chinese Journal of Ecology,20(4):5-8.(in Chinese)
[19]陈建勋.植物生理学实验指导[M].广州:华南理工大学出版社,2002.CHEN Jian-xun.(2002).Plant physiology experiment[M].Guangzhou:South China University of Technology Press.(in Chinese)
[20]国家种子检验协会(ISTA).1996国际种子检验规程[M].北京:中国农业出版社,1999National Institute of Seed Testing(1999).1996 International Seed Testing Procedures[M].Beijing:China Agriculture Press.(in Chinese)
[21]王学奎.植物生理生化实验原理和技术[M].北京:高等教育出版社,2006:134-136,280-288.WANG Xue-kui.(2006).Experimental principle and technology of plant physiology and Biochemistry[M].Beijing:Higher Education Press:134-136,280-288.(in Chinese)
[22]马博英.铅、锌诱导的高羊茅叶片过氧化物酶活性变化[J].浙江教育学院学报,2008,(4):77-81.MA Bo-ying.(2008).The changes of peroxidase activities in tall fescue leaves induced by lead and zinc[J].Journal of Zhejiang College of Education,(4):77-81.(in Chinese)
[23]韩金玲,马春英,杨晴,等.施锌对冬小麦品质性状的影响[J].麦类作物学报,2007,27(1):112-115.HAN Jin-ling,MA Chun-ying,YANG Qing,et al.(2007).Effect of zinc on quality traits of Winter Wheat[J].Journal of Triticeae Crops,27(1):112-115.(in Chinese)
[24]张春荣,李红,夏立江,等.镉、锌对紫花苜蓿种子萌发及幼苗的影响[J].华北农学报,2005,20(1):96-99.ZHANG Chun-rong,LI Hong,XIA Li-jiang,et al.(2005).Effects of zinc cadmium germination of medicago sativa seeds and growth of seedling[J].Acta Agriculturae Boreali-Sinica,20(1):96-99.(in Chinese)
[25]王友保,刘登义.Cu、As及其复合污染对小麦生理生态指标的影响[J].应用生态学报,2001,12(5):773-776.WANG You-bao,LIU Deng-yi.(2001).Effect of Cu,As and combined pollution on eco physiological index of wheat[J].Journal of Applied Ecology,12(5):773-776.(in Chinese)
[26]朱红霞,杨小勇,葛才林,等.重金属对水稻过氧化物酶同工酶的影响[J].核农学报,2004,18(3):233-236.ZHU Hong-xia,YANG Xiao-yong,GE Cai-lin,et al.(2004).Effects of heavy metals on the peroxidase isozyme of rice[J].Journal of Nuclear Agriculture,18(3):233-236.(in Chinese)
[27]周红卫,施国新,陈景耀,等.6-BA对水花生抗氧化酶系Hg2+毒害的缓解作用[J].生态学报,2003,23(2):387-392.ZHOU hong-wei,SHI guo-xin,CHEN jing-yao,et al.To ease the effects of 6-BA on antioxidant enzymes of Hg2+toxicity in alternanthera philoxeroides[J].Ecological Journal,2003,23(2):387-392.(in Chinese)
[2]王夔.生命科学中的微量元素(上卷)[M].北京:中国计量出版社,1991:140-141.WANG Kui.(1991).Life Science Trace Elements(volume)[M].Beijing:China Metrology Press:140-141.(in Chinese)
[3]龚红梅,李卫国.锌对植物的毒害及机理研究进展[J].安徽农业科学,2009,37(29):14 009-14 015.GONG Hong-mei,LI Wei-guo.(2009).Research Progress on toxicity and mechanism of zinc on plants[J].Anhui Agricultural Sciences,37(29):14,009-14,015.(in Chinese)
[4]何中虎,夏先春,罗晶,等.国际小麦育种研究趋势分析[J].麦类作物学报,2006,26(2):154-156.HE Zhong-hu,XIA Xian-chun,LUO Jing,et al.(2006).Analysis of international wheat breeding trend[J].Journal of Triticeae Crops,26(2):154-156.(in Chinese)
[5]王荣栋,孔军,陈荣毅,等.新疆小麦品质生态区划[J].新疆农业科学,2005,42(5):309-314.WANG Rong-dong,KONG Jun,CHEN Rong-yi,et al.(2005).Ecological regionalization of wheat quality in Xinjiang[J].Xinjiang Agricultural Sciences,42(5):309-314.(in Chinese)
[6]林存亮,李鸿文,石培春,等.黑小麦品种(系)加工品质性状和蒸煮食品加工品质特性研究[J].新疆农业科学,2012,49(4):610-616.LIN Cun-liang,LI Hong-wen,SHI Pei-chun,et al.(2012).Studies on processing quality traits and cooking food processing quality characteristics of black wheat varieties(lines)[J].Xinjiang Agricultural Sciences,49(4):610-616.(in Chinese)
[7]袁敏,铁柏清,唐美珍,等.4种草对铅锌尾矿污染土壤重金属的抗性与吸收特性[J].生态环境,2005,14(1):43-47.YUAN Min,TIE Bai-qing,TANG Mei-zhen,et al.(2005).Resistance and uptake of four grass soil heavy metal pollution in the lead-zinc tailings[J].Ecological Environment,14(1):43-47.(in Chinese)
[8]李文一,徐卫红,胡小风,等.Zn胁迫对黑麦草幼苗生长、生理生化及Zn吸收的影响[J].农业工程学报,2007,23(5):190-194.LI Wen-yi,XU Wei-hong,HU Xiao-feng,et al.(2007).Zn stress,physiological and biochemical effects of growth and Zn uptake of Ryegrass Seedlings[J].Transactions of the Chinese Society of Agricultural Engineering,23(5):190-194.(in Chinese)
[9]齐国辉,李保国,郭军,等.锌胁迫与缺锌对西府海棠叶片保护酶活性及膜质过氧化的影响[J].河北林果研究,2006,21(4):409-411.QI Guo-hui,LI Bao-guo,GUO Jun,et al.(2006).Effects of zinc stress and zinc deficiency on protective enzyme activity and membrane lipid peroxidation in leaves of Malus spectabilis[J].Hebei Fruit Research,21(4):409-411.(in Chinese)
[10]邱昌恩,毕永红,胡征宇.Zn2+胁迫对绿球藻生长、生理特性及细胞结构的影响[J].水生生物学报,2007,31(4):503-508.QIU Chang-en,BI Yong-hong,HU Zheng-yu.(2007).Zn2+stress effects on growth,physiological characteristics and cell structure of the green algae[J].Journal of Aquatic Biology,31(4):503-508.(in Chinese)
[11]杨科璧.中国农田土壤重金属污染与其植物修复研究[J].世界农业,2007,(8):58-61.YANG Ke-bi.(2007).Heavy metal pollution of soil in China and its phytoremediation[J].World Agriculture,(8):58-61.(in Chinese)
[12]丁海东,齐乃敏,朱为民,等.镉、锌胁迫对番茄幼苗生长及其脯氨酸与谷胱甘肽含量的影响[J].中国生态农业学报,2006,14(2):53-55.DING Hai-dong,QI Nai-min,ZHU Wei-min,et al.(2006).Cadmium and zinc stress on Tomato Seedling Growth and proline content and glutathione[J].Chinese Journal of Ecological Agriculture,14(2):53-55.(in Chinese)
[13]张远兵,刘爱荣,董建国,等.9个高羊茅品种种子萌发和幼苗生长对锌胁迫的响应[J].中国生态农业学报,2010,18(2):371-376.ZHANG Yuan-bing,LIU Ai-rong,DONG Jian-guo,et al.(2010).Nine varieties of tall fescue seed germination and seedling growth response to zinc stress[J].Chinese Journal of Ecological Agriculture,18(2):371-376.(in Chinese)
[14]齐笑笑,肖家欣,徐春丽,等.锌胁迫对3种柑橘砧木的生理特性和锌分配的影响[J].中国农学通报,2009,25(24):313QI Xiao-xiao,XIAO Jia-xin,XU Chun-li,et al.(2009).Effects of zinc stress on physiological characteristics and zinc distribution of three species of Citrus[J].Chinese Agricultural Bulletin,25(24):313-317.(in Chinese)
[15]张莹,魏安智,杨途熙,等.锌胁迫对灰杨幼苗生长和光合特性的影响[J].东北林业大学学报,2011,39(3);19-21.ZHANG Ying,WEI An-zhi,YANG Tu-xi,et al.(2011).Effects of zinc stress on growth and photosynthetic characteristics of Populus seedlings[J].Journal of Northeast Forestry University,39(3):19-21.(in Chinese)
[16]黄永杰,杨红飞,张栋,等.锌胁迫对水花生生长和土壤酶活性的影响[J].上海交通大学学报,2009,27(4):403-408.HUANG yong-jie,YANG hong-fei,ZHANG dong,et al.(2009).Effects of zinc stress on growth and activity of soil enzyme of Alternanthera philoxeroides[J].Journal of Shanghai Jiaotong University,27(4):403-408.(in Chinese)
[17]宗宪春,宗灿华,王书臻,等.锌胁迫对虞美人种子萌发的影响[J].安徽农业科学,2011,39(33):20 371-20 372.ZONG Xian-chun,ZONG Can-hua,WANG Shu-zhen,et al.(2011).Effects of zinc stress on the germination of poppy seed[J].Anhui Agricultural Sciences,39(33):20,371-20,372.(in Chinese)
[18]常学秀,文传浩,沈其荣,等.锌厂Pb污染农田小麦根际与非根际土壤酶活性特征研究[J].生态学杂志,2001,20(4):5-8.CHANG Xue-xiu,WEN Chuan-hao,SHEN Qi-rong,et al.(2001).Characteristics of soil enzyme activity in the rhizosphere and non-rhizosphere soil of Pb polluted by zinc plant[J].Chinese Journal of Ecology,20(4):5-8.(in Chinese)
[19]陈建勋.植物生理学实验指导[M].广州:华南理工大学出版社,2002.CHEN Jian-xun.(2002).Plant physiology experiment[M].Guangzhou:South China University of Technology Press.(in Chinese)
[20]国家种子检验协会(ISTA).1996国际种子检验规程[M].北京:中国农业出版社,1999National Institute of Seed Testing(1999).1996 International Seed Testing Procedures[M].Beijing:China Agriculture Press.(in Chinese)
[21]王学奎.植物生理生化实验原理和技术[M].北京:高等教育出版社,2006:134-136,280-288.WANG Xue-kui.(2006).Experimental principle and technology of plant physiology and Biochemistry[M].Beijing:Higher Education Press:134-136,280-288.(in Chinese)
[22]马博英.铅、锌诱导的高羊茅叶片过氧化物酶活性变化[J].浙江教育学院学报,2008,(4):77-81.MA Bo-ying.(2008).The changes of peroxidase activities in tall fescue leaves induced by lead and zinc[J].Journal of Zhejiang College of Education,(4):77-81.(in Chinese)
[23]韩金玲,马春英,杨晴,等.施锌对冬小麦品质性状的影响[J].麦类作物学报,2007,27(1):112-115.HAN Jin-ling,MA Chun-ying,YANG Qing,et al.(2007).Effect of zinc on quality traits of Winter Wheat[J].Journal of Triticeae Crops,27(1):112-115.(in Chinese)
[24]张春荣,李红,夏立江,等.镉、锌对紫花苜蓿种子萌发及幼苗的影响[J].华北农学报,2005,20(1):96-99.ZHANG Chun-rong,LI Hong,XIA Li-jiang,et al.(2005).Effects of zinc cadmium germination of medicago sativa seeds and growth of seedling[J].Acta Agriculturae Boreali-Sinica,20(1):96-99.(in Chinese)
[25]王友保,刘登义.Cu、As及其复合污染对小麦生理生态指标的影响[J].应用生态学报,2001,12(5):773-776.WANG You-bao,LIU Deng-yi.(2001).Effect of Cu,As and combined pollution on eco physiological index of wheat[J].Journal of Applied Ecology,12(5):773-776.(in Chinese)
[26]朱红霞,杨小勇,葛才林,等.重金属对水稻过氧化物酶同工酶的影响[J].核农学报,2004,18(3):233-236.ZHU Hong-xia,YANG Xiao-yong,GE Cai-lin,et al.(2004).Effects of heavy metals on the peroxidase isozyme of rice[J].Journal of Nuclear Agriculture,18(3):233-236.(in Chinese)
[27]周红卫,施国新,陈景耀,等.6-BA对水花生抗氧化酶系Hg2+毒害的缓解作用[J].生态学报,2003,23(2):387-392.ZHOU hong-wei,SHI guo-xin,CHEN jing-yao,et al.To ease the effects of 6-BA on antioxidant enzymes of Hg2+toxicity in alternanthera philoxeroides[J].Ecological Journal,2003,23(2):387-392.(in Chinese)