甘肃省定西市马铃薯疮痂病新病原Streptomyces galilaeus的分离、鉴定及生物学特性研究
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摘要
对甘肃省定西市安定区的马铃薯疮痂病病原进行了分离、鉴定和生物学特性研究。结果表明,菌株5T-1具有较强致病性,菌落表面呈灰色,有金属光泽,平均直径为4.68mm,可产生黄褐色素,孢子圆形或圆柱形,孢子丝松散,革兰氏染色呈阳性。5T-1的16SrDNA序列与加利利链霉菌Streptomyces galilaeus菌株的相似度为99%,结合形态特征将菌株5T-1鉴定为Streptomyces galilaeus,为甘肃省新报道病原菌。菌株5T-1生长最适温度为30℃,最适光照条件为全黑暗,最适pH为8.5,最佳碳源和氮源分别为肌醇和天冬氨酸。该研究结果为甘肃省马铃薯疮痂病诊断和综合防治提供了依据。
The pathogen of potato scab from Anding District of Dingxi City in Gansu Province was isolated and identified,and its biological characteristics were also determined.The results showed that the strain 5 T-1 was highly pathogenic.The colony was gray with a metallic luster and the average diameter of 4.68 mm,which could produce yellow brown pigment.The spores were round or cylindrical and spores silk was loose.Gram staining is positive.The similarity of 16 S rDNA sequence of 5 T-1 to that of Streptomyces galilaeus was 99%.Combining with the morphological characteristics,the strain5 T-1 was identified as S.galilaeus.It is a newly reported pathogen in Gansu Province.The optimal temperature for strain growth was 30℃.The optimal illumination conditions were darkness and the optimal pH value was 8.5.The best carbon source and nitrogen source were inositol and aspartic acid,respectively.The results provide a basis for the diagnosis and integrated control of potato scab in Gansu Province.
The pathogen of potato scab from Anding District of Dingxi City in Gansu Province was isolated and identified,and its biological characteristics were also determined.The results showed that the strain 5 T-1 was highly pathogenic.The colony was gray with a metallic luster and the average diameter of 4.68 mm,which could produce yellow brown pigment.The spores were round or cylindrical and spores silk was loose.Gram staining is positive.The similarity of 16 S rDNA sequence of 5 T-1 to that of Streptomyces galilaeus was 99%.Combining with the morphological characteristics,the strain5 T-1 was identified as S.galilaeus.It is a newly reported pathogen in Gansu Province.The optimal temperature for strain growth was 30℃.The optimal illumination conditions were darkness and the optimal pH value was 8.5.The best carbon source and nitrogen source were inositol and aspartic acid,respectively.The results provide a basis for the diagnosis and integrated control of potato scab in Gansu Province.
引文
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[9]邢莹莹,吕典秋,魏琪,等.黑龙江省部分地区马铃薯疮痂病菌种类及致病性鉴定[J].植物保护,2016,42(1):26-32.
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[12]MIYAJIMA K,TANAKA F,TAKEUCHI T.Streptomyces turgidiscabies sp.nov.[J].International Journal of Systematic Bacteriology,1998,2(2):495-502.
[13]杨梦平,王瑞仙,杜魏甫,等.云南省马铃薯疮痂病致病链霉菌种类组成研究[J].植物病理学报,2018,48(4):445-454.
[14]李拴曹,李存玲.马铃薯疮痂病的发生与防治[J].陕西农业科学,2016,62(1):76-77.
[15]张萌,刘伯,于秀梅,等.中国马铃薯疮痂病菌生物学特性分析[J].中国农业科学,2010,43(12):2603-2610.
[2]LORIA R,BUKHALID R A,FRY B A.Plant pathogenicity in the genus Streptomyces[J].Plant Disease,1997,81:836-846.
[3]PARK D H,YU Y M,KIM J S,et al.Characterization of Streptomycetes causing potato common scab in Korea[J].Plant Disease,2003,87:1290-1296.
[4]WANNER L A.A survey of genetic variation in Streptomyces isolates causing potato common scab in the United States[J].Phytopathology,2006,96:1363-1371.
[5]KREUZE J F,SUOMALAINEN S,PAULIN L,et al.Phylogenetic analysis of 16SrRNA genes and PCR analysis of the necl gene fromStreptomyces spp.causing common scab,pitted scab,and netted scab in Finland[J].Phytopathology,1999,89:462-469.
[6]赵伟全,杨文香,李亚宁,等.中国马铃薯疮痂病菌的鉴定[J].中国农业科学,2006,39(2):313-318.
[7]张萌,赵伟全,于秀梅,等.中国马铃薯疮痂病病原菌16SrDNA的遗传多样性分析[J].中国农业科学,2009,42(2):499-504.
[8]康蓉,王生荣.甘肃马铃薯疮痂病病原初步鉴定[J].植物保护,2013,39(3):78-82.
[9]邢莹莹,吕典秋,魏琪,等.黑龙江省部分地区马铃薯疮痂病菌种类及致病性鉴定[J].植物保护,2016,42(1):26-32.
[10]GOYER C,BEAULIEU C.Host range of streptomyces strains causing common scab[J].Plant Disease,1997,81(8):901-904.
[11]LAMBERT D H,LORIA R.Streptomyces acidiscabies sp.nov.[J].International Journal of Systematic Bacteriology,1989,39(4):393-396.
[12]MIYAJIMA K,TANAKA F,TAKEUCHI T.Streptomyces turgidiscabies sp.nov.[J].International Journal of Systematic Bacteriology,1998,2(2):495-502.
[13]杨梦平,王瑞仙,杜魏甫,等.云南省马铃薯疮痂病致病链霉菌种类组成研究[J].植物病理学报,2018,48(4):445-454.
[14]李拴曹,李存玲.马铃薯疮痂病的发生与防治[J].陕西农业科学,2016,62(1):76-77.
[15]张萌,刘伯,于秀梅,等.中国马铃薯疮痂病菌生物学特性分析[J].中国农业科学,2010,43(12):2603-2610.