灵芝发酵粉中三萜化合物提取与测定
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摘要
试验采用超声波辅助提取灵芝发酵粉中三萜类化合物,运用分光光度法测定其得率。通过单因素和BoxBenhnken设计研究乙醇浓度、浸泡时间、料液比、超声时间、超声功率、超声温度对灵芝三萜类化合物得率的影响。研究结果表明,灵芝三萜化合物提取的最佳工艺参数为:料液比为1∶42(g/m L),95%乙醇溶液中浸泡100 min后,超声功率为210 W下43℃超声提取17 min。在最优工艺条件下进行3次验证试验,测得三萜化合物平均得率为0.612 8 mg/g。研究结果为灵芝发酵类产品的在线监测提供理论依据。
Triterpene compounds in Ganoderma lucidum powder were extracted by ultrasonic wave and the yield was determined by spectrophotometry. The effects of ethanol concentration,soaking time,solid-liquid ratio,ultrasonic time,ultrasonic power and ultrasonic temperature on the yield of Ganoderma lucidum triterpenoids were studied respectively. Based on the single-factor experiments,and ultrasonic power as significant factors,according to the design principle of Box-benhnken,the response surface of the experiment optimization design. The optimal process parameters were as follows:the ratio of solid to liquid was 1 ∶ 42(g/m L),immersed in 95 % ethanol for 100 min,the temperature of ultrasound was 43 ℃,and the ultrasonic power was 210 W for17 min. validation experiments were carried out at these optimum conditions,the average yield of triterpenoids measured was 0.612 8 mg/g.
Triterpene compounds in Ganoderma lucidum powder were extracted by ultrasonic wave and the yield was determined by spectrophotometry. The effects of ethanol concentration,soaking time,solid-liquid ratio,ultrasonic time,ultrasonic power and ultrasonic temperature on the yield of Ganoderma lucidum triterpenoids were studied respectively. Based on the single-factor experiments,and ultrasonic power as significant factors,according to the design principle of Box-benhnken,the response surface of the experiment optimization design. The optimal process parameters were as follows:the ratio of solid to liquid was 1 ∶ 42(g/m L),immersed in 95 % ethanol for 100 min,the temperature of ultrasound was 43 ℃,and the ultrasonic power was 210 W for17 min. validation experiments were carried out at these optimum conditions,the average yield of triterpenoids measured was 0.612 8 mg/g.
引文
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[15]姚松君,黄生权,陈壮耀,等.超声辅助提取灵芝三萜的工艺研究[J].现代食品科技,2009,25(10):1220-1223
[16]宋师花,贾晓斌,陈彦,等.超临界CO2萃取灵芝子实体中的三萜类成分[J].中国中药杂志,2008,33(17):2104-2107
[17]陈飞,马闽卉,梁运祥,等.不同品种灵芝固态发酵菌丝体多糖和三萜含量的比较[J].食品科技,2011,36(9):19-22
[18]贾瑞博,赵慧,刘凯丽,等.树舌灵芝总三萜提取工艺优化及其抗氧化作用研究[J].应用化工,2016,45(6):1030-1035
[2]王明蛟,高航,刚婉娇.灵芝化学成分分析方法的研究[J].长春中医药大学学报,2014,30(5):817-819
[3]Liu RM,Zhong JJ.Ganoderic acid Mf and S induce mitochondria mediated apoptosis in human cervical carcinoma He La cells[J].Phytomedicine,2011,18(5):349-355
[4]王晓玲,刘高强,周国英.紫芝发酵菌体中三萜类化合物的抑菌活性研究[J].时珍国医国药,2008,19(11):2636-2637
[5]Shwu Bin Lin,Chyi Hann Li,Shiuh Sheng Lee,et al.Triterpene-enriched extracts from Ganoderma lucidum inhibit growth of hepatoma cells via suppressing protein kinase C,activating mitogen-activated protein kinases and G2-phase cell cycle arrest[J].Life Sciences,2003,72(21):2381-2390
[6]刘峰,李鹏,黄秀旺,等.灵芝三萜组分GLE体内外抗肿瘤作用[J].中国新药杂志,2012(23):2790-2793
[7]Yue QX,Xie FB,Guan SH,et al.Interaction of Ganoderma triterpenes with doxorubicin and proteomic characterization of the possible molecular targets of Ganoderma triterpenes[J].Cancer Science,2010,99(7):1461-1470
[8]刘莉莹,王洪庆,刘超,等.树灵芝中三萜类成分及其保肝作用研究[J].天然产物研究与开发,2017(4):584-589
[9]刘晓珍,聂少平,李文娟,等.黑灵芝中性提取物三萜类含量测定及抗氧化作用研究[J].南昌大学学报,2011,33(4):332-337
[10]彭珍华,韩本,赵声兰,等.三萜类化合物抗HIV研究进展[J].天然产物研究与开发,2009,21(5):258-262
[11]Hong Huey Ko,Chi Feng Hung,Jih Pyang wang,et al.Antiinflammatory triterpenoids and steroids from Ganoderma Lucidum and G.tsugae[J].Phytochemistry,2008,69(1):234-239
[12]陈慧,王晓玲,刘高强.灵芝子实体中三萜酸的提取及其稳定性[J].食用菌学报,2015,22(4):58-64
[13]蒉霄云,何晋浙,王静,等.微波提取灵芝中三萜类化合物的研究[J].中国食品学报,2010,10(2):89-96
[14]洪文龙,童斌,赵格.响应面法优化灵芝子实体三萜提取工艺的研究[J].食品研究与开发,2014(11):42-46
[15]姚松君,黄生权,陈壮耀,等.超声辅助提取灵芝三萜的工艺研究[J].现代食品科技,2009,25(10):1220-1223
[16]宋师花,贾晓斌,陈彦,等.超临界CO2萃取灵芝子实体中的三萜类成分[J].中国中药杂志,2008,33(17):2104-2107
[17]陈飞,马闽卉,梁运祥,等.不同品种灵芝固态发酵菌丝体多糖和三萜含量的比较[J].食品科技,2011,36(9):19-22
[18]贾瑞博,赵慧,刘凯丽,等.树舌灵芝总三萜提取工艺优化及其抗氧化作用研究[J].应用化工,2016,45(6):1030-1035