ICP-OES法定性定量分析野生鸡枞菌中的矿质元素组成
|
摘要
建立一种微波消解-全谱直读电感耦合等离子体发射光谱(ICP-OES)定性定量分析方法,检测野生鸡枞菌子实体中的矿质元素组成。应用浓硝酸-过氧化氢湿法微波消解样品,通过ICP-OES测定得到的发射光谱图、信号强度、背景强度、RSD值等数据,对69种元素进行定性分析。结果显示,鸡枞菌中含17种矿质元素。对17种元素配制合适浓度的系列标准溶液,利用ICP-OES测定相应发射光谱强度,绘制信号强度与浓度标准工作曲线,外标法定量。定量结果显示, ICP-OES定量分析线性范围在2个数量级时,工作曲线线性相关系数在0.999以上;样品测定RSD值为0.51%～9.87%,大部分在5%以内;回收率为91.2%～109.5%;鸡枞菌中含量较高、大于1 000 mg/kg的矿质元素有P、S、K、Si、Al、Mg、Fe,含量在200～500 mg/kg的元素有Ca和Na,含量在10～50 mg/kg的元素有Mn、Ti、Zn、Ba,含量甚微、小于10 mg/kg的元素有B、Cd、Sr、Cu。分析结果可为相关应用研究提供参考数据。
A qualitative and quantitative analysis method was constructed by microwave digestion directreading inductively coupled plasma atomic emission spectrometry(ICP-OES), and the mineral elements in wild Termitornyces albuminosus(Berk) Heim were then determined. The samples were digested by wet microwave digestion with solvents of concentrated nitric acid and hydrogen peroxide. The 69 elements were analyzed qualitatively by ICP-OES emission spectrum, signal intensity, background intensity and RSD value of these data. The qualitative results showed that about 17 kinds of mineral elements were contained in Termitomyces albuminosus. A series of standard solutions of suitable concentrations of the 17 elements were prepared. The corresponding emission spectrum intensity was also measured by ICP-OES and the external standard method was used for quantitative analysis. The standard working curve of concentration was plotted against signal intensity. The quantitative results showed that the ICP-OES quantitative analysis linear range was among 2 order magnitude and the correlation coefficient of the standard curve was more than 0.999. The RSD value was between 0.51% and 9.87%, most of which was within 5%. The recovery rate was between 91.2% and 109.5%. The content of mineral elements of P, S, K, Si Al, Mg and Fe was more than 1 000 mg/kg; That of Ca and Na was between 200 and 500 mg/kg; That of Mn, Ti, Zn and Ba was between 10 and 50 mg/kg; That of B, Cd, Sr and Cu was less than 10 mg/kg. The analysis results provide reference data for relevant application research.
A qualitative and quantitative analysis method was constructed by microwave digestion directreading inductively coupled plasma atomic emission spectrometry(ICP-OES), and the mineral elements in wild Termitornyces albuminosus(Berk) Heim were then determined. The samples were digested by wet microwave digestion with solvents of concentrated nitric acid and hydrogen peroxide. The 69 elements were analyzed qualitatively by ICP-OES emission spectrum, signal intensity, background intensity and RSD value of these data. The qualitative results showed that about 17 kinds of mineral elements were contained in Termitomyces albuminosus. A series of standard solutions of suitable concentrations of the 17 elements were prepared. The corresponding emission spectrum intensity was also measured by ICP-OES and the external standard method was used for quantitative analysis. The standard working curve of concentration was plotted against signal intensity. The quantitative results showed that the ICP-OES quantitative analysis linear range was among 2 order magnitude and the correlation coefficient of the standard curve was more than 0.999. The RSD value was between 0.51% and 9.87%, most of which was within 5%. The recovery rate was between 91.2% and 109.5%. The content of mineral elements of P, S, K, Si Al, Mg and Fe was more than 1 000 mg/kg; That of Ca and Na was between 200 and 500 mg/kg; That of Mn, Ti, Zn and Ba was between 10 and 50 mg/kg; That of B, Cd, Sr and Cu was less than 10 mg/kg. The analysis results provide reference data for relevant application research.
引文
[1]靳敏,夏玉宇.食品检验技术[M].北京:化学工业出版社,2003:360.
[2]中国营养学会.中国居民膳食营养素参考摄入量[M].北京:中国轻工业出版社, 2010:179.
[3]张玉金,李荣春.鸡菌与白蚁的共生专一性初步研究[J].食用菌学报, 2017, 24(3):79-86.
[4]付子艳,李荣春.鸡枞菌属与华鸡枞菌属分类研究现状[J].广西植物, 2009, 29(1):32-38.
[5]龙正海,曾大兴.鸡枞菌培养特性的初步研究[J].食品与生物技术学报, 2007, 26(3):90-94.
[6]施渺筱,李祝,杨坤耀,等.野生鸡枞菌子实体不同部位营养成分分析[J].食品研究与开发, 2012, 33(2):182-183.
[7]邹立扣,潘欣,岳爱玲,等.四川省鸡枞菌氨基酸组成及硒元素含量分析[J].食品科学, 2011, 32(14):245-248.
[8]吴素蕊,严明,张鑫,等.响应面法优化水提黑皮鸡枞菌多糖工艺[J].食品工业, 2016, 37(5):169-172.
[9]马雅鸽,马亚宁,张晓娟.鸡枞菌总多酚提取工艺的优化[J].食品工业, 2015, 36(6):209-211.
[10]赵君威,梅坛,鄢国强,等.电感耦合等离子体原子发射光谱分析中的光谱干扰及其校正的研究进展[J].理化检验(化学分册), 2013, 49(3):364-369.
[11]郑国经.电感耦合等离子体原子发射光谱分析仪器与方法的新进展[J].冶金分析, 2014, 34(11):1-10.
[12]张玉金,张静潮,李荣春,等. 2种鸡菌营养成分分析[J].食品科学, 2014, 35(14):159-164.
[13]肖洋,李祝,施渺筱.粗柄鸡枞菌子实体不同部位营养成分及重金属测定[J].中国酿造, 2014 33(4):142-144.
[14] KULIKOV E.二乙烯三胺五乙酸(DTPA)提取方法结合Agilent 5110 SVDV ICP-OES测定土壤中的营养元素[J].环境化学, 2016, 35(5):1090-1092.
[2]中国营养学会.中国居民膳食营养素参考摄入量[M].北京:中国轻工业出版社, 2010:179.
[3]张玉金,李荣春.鸡菌与白蚁的共生专一性初步研究[J].食用菌学报, 2017, 24(3):79-86.
[4]付子艳,李荣春.鸡枞菌属与华鸡枞菌属分类研究现状[J].广西植物, 2009, 29(1):32-38.
[5]龙正海,曾大兴.鸡枞菌培养特性的初步研究[J].食品与生物技术学报, 2007, 26(3):90-94.
[6]施渺筱,李祝,杨坤耀,等.野生鸡枞菌子实体不同部位营养成分分析[J].食品研究与开发, 2012, 33(2):182-183.
[7]邹立扣,潘欣,岳爱玲,等.四川省鸡枞菌氨基酸组成及硒元素含量分析[J].食品科学, 2011, 32(14):245-248.
[8]吴素蕊,严明,张鑫,等.响应面法优化水提黑皮鸡枞菌多糖工艺[J].食品工业, 2016, 37(5):169-172.
[9]马雅鸽,马亚宁,张晓娟.鸡枞菌总多酚提取工艺的优化[J].食品工业, 2015, 36(6):209-211.
[10]赵君威,梅坛,鄢国强,等.电感耦合等离子体原子发射光谱分析中的光谱干扰及其校正的研究进展[J].理化检验(化学分册), 2013, 49(3):364-369.
[11]郑国经.电感耦合等离子体原子发射光谱分析仪器与方法的新进展[J].冶金分析, 2014, 34(11):1-10.
[12]张玉金,张静潮,李荣春,等. 2种鸡菌营养成分分析[J].食品科学, 2014, 35(14):159-164.
[13]肖洋,李祝,施渺筱.粗柄鸡枞菌子实体不同部位营养成分及重金属测定[J].中国酿造, 2014 33(4):142-144.
[14] KULIKOV E.二乙烯三胺五乙酸(DTPA)提取方法结合Agilent 5110 SVDV ICP-OES测定土壤中的营养元素[J].环境化学, 2016, 35(5):1090-1092.