江西亚洲柑橘木虱的种群遗传结构研究
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摘要
亚洲柑橘木虱Diaphorina citri Kuwayama是芸香科Rutaceae植物新梢期的重要害虫,也是黄龙病Huanglongbing的主要传播媒介,严重威胁江西柑橘产业的发展。本文利用线粒体基因标记,研究江西10个地点亚洲柑橘木虱的种群遗传结构和多态性。扩增了2个线粒体COⅠ基因片段,长度为1 431 bp。其中多态性位点2个(均为非同义突变,A/G转换),单倍型3个(Hap-1,Hap-2和Hap-3)。最常见的单倍型是Hap-1(占总样本数的91.7%),发生在所有采样点;其次为Hap-3(6.3%),分布在大余和崇义;Hap-2(2.0%)仅在兴国出现。单一直线型的单倍型网络结构模式、小而不显著的HR(Harpending’s raggedness)指数值,以及单峰型错配分布曲线均表明:江西地区的亚洲柑橘木虱是近期入侵并经历了快速的种群动态扩张。
The Asian citrus psyllid Diaphorina citri Kuwayama(Hemiptera: Liviidae), an important pest of the young developing leaves of Rutaceae, is the main vector of the bacterium that causes citrus greening disease(huanglongbing). The population genetic structure and polymorphism of D.citri from 10 sites in Jiangxi were studied by using mitochondrial markers. Two fragments of mitochondrial CO Ⅰ sequences were amplified with a total length of 1 431 bp. We found 2 polymorphic sites(nonsynonymous, A/G transition) and 3 haplotypes(Hap-1,Hap-2 and Hap-3). Among these, the most common haplotype was Hap-1(91.7% of the total) occurring at all sampling sites. Hap-3(6.3%) was distributed in Dayu and Chongyi. Hap-2(2%) was observed only in Xingguo. The single-line haplotype network model, the small and insignificant Harpending's raggedness(HR) index and the unimodal mismatch distribution curve indicated that D.citri populations in Jiangxi had the recent invasion and rapid dynamic expansion.
The Asian citrus psyllid Diaphorina citri Kuwayama(Hemiptera: Liviidae), an important pest of the young developing leaves of Rutaceae, is the main vector of the bacterium that causes citrus greening disease(huanglongbing). The population genetic structure and polymorphism of D.citri from 10 sites in Jiangxi were studied by using mitochondrial markers. Two fragments of mitochondrial CO Ⅰ sequences were amplified with a total length of 1 431 bp. We found 2 polymorphic sites(nonsynonymous, A/G transition) and 3 haplotypes(Hap-1,Hap-2 and Hap-3). Among these, the most common haplotype was Hap-1(91.7% of the total) occurring at all sampling sites. Hap-3(6.3%) was distributed in Dayu and Chongyi. Hap-2(2%) was observed only in Xingguo. The single-line haplotype network model, the small and insignificant Harpending's raggedness(HR) index and the unimodal mismatch distribution curve indicated that D.citri populations in Jiangxi had the recent invasion and rapid dynamic expansion.
引文
[1]YANG Y,HUANG M,BEATTIE G A C,et al.Distribution,biology,ecology and control of the psyllid Diaphorina citri Kuwayama,a major pest of citrus:A status report for China[J].International Journal of Pest Management,2006,52:343-352.
[2]邓明学.以控制木虱为重点的柑桔黄龙病综合防治理论的形成过程、依据和技术要点[J].中国农学通报,2009,25(23):358-363.
[3]DA GRAA J V.Biology,history,and world status of Huanglongbing[C]∥Taller International sobre Huanglongbing de los cítricos(Candidatus Liberibacter spp.)yel psílido asiático de los cítricos(Diaphorina citri),7-9May 2008,Hermosillo,Sonora,Mexico.
[4]HALBERT S E,MANJUNATH K L.Asian citrus psyllids(Sternorrhyncha:Psyllidae)and greening disease of citrus:a literature review and assessment of risk in Florida[J].Florida Entomologist,2004,87:330-353.
[5]HOLLIS D.A new citrus-feeding psyllid from the Comoro Islands,with a review of the Diaphorina amoena species group(Homoptera)[J].Systematic Entomology,1987,12:47-61.
[6]WATERHOUSE D F.Biological control of insect pests:Southeast Asian prospects[M].ACIAR Monograph Series No.51.Canberra:Australian Centre for International Agricultural Research.1998.
[7]HALBERT S E,NU'EZ C A.Distribution of the Asian citrus psyllid Diaphorina citri Kuwayama(Rhynchota:Psyllidae)in the Caribbean basin[J].Florida Entomologist,2004,87:401-402.
[8]LUO Y F,AGNARSSON I.Global mtDNA genetic structure and hypothesized invasion history of a major pest of citrus,Diaphorina citri(Hemiptera:Liviidae)[J].Ecology and Evolution,2018,8:257-265.
[9]ZHAO X.Occurrence of citrus yellow shoot(greening)disease in Guangxi and southern Sichuan:a brief review[C]∥The FAO-UNDP Project Coordinator in Fuzhou.Regional workshop on citrus greening huanglongbing disease,6-12December 1987,Fuzhou,China.
[10]YANG Y.Effects on light,temperature and humidity on the development,reproduction and survival of citrus psylla[J].Acta Ecologica Sinica,1989,9:348-354.
[11]XU C,XIA Y,KE C.Study on the biology and control of citrus psylla[J].Acta Phytophylacica Sinica,1994,21:53-56.
[12]WANG Z.Distribution and transmission of citrus psylla in Guizhou Province[J].Tillage and Cultivation,2002,22(4):62-63.
[13]CARMICHAEL L E,KRIZAN J,NAGY J A,et al.Historical and ecological determinants of genetic structure in arctic canids[J].Molecular Ecology,2007,16:3466-3483.
[14]PINHO C,HARRIS D J,FERRAND N.Comparing patterns of nuclear and mitochondrial divergence in a cryptic species complex:the case of Iberian and North African wall lizards(Podarcis,Lacertidae)[J].Botanical Journal of the Linnean Society,2007,91:121-133.
[15]JOURDIE V,ALVAREZ N,MOLINA-OCHOA J,et al.Population genetic structure of two primary parasitoids of Spodoptera frugiperda(Lepidoptera),Chelonus insularis and Campoletis sonorensis(Hymenoptera):to what extent is the host plant important?[J].Molecular Ecology,2010,19:2168-2179.
[16]REMAIS J V,XIAO N,AKULLIAN A.Genetic assignment methods for gaining insight into the management of infectious disease by understanding pathogen,vector,and host movement[J/OL].PLoS Pathogens,2011,7:4.
[17]BOYKIN L M,DE BARRO P,HALL D G,et al.Overview of worldwide diversity of Diaphorina citri Kuwayama mitochondrial cytochrome oxidase I haplotypes:two Old World lineages and a New World invasion[J].Bulletin of Entomological Research,2012,102:573-582.
[18]GUIDOLIN A S,FRESIA P,CNSOLI F L.The genetic structure of an invasive pest,the Asian citrus psyllid Diaphorina citri(Hemiptera:Liviidae)[J/OL].PLoS ONE,2014,9:e115749.
[19]LASHKARI M,MANZARI S,SAHRAGARD A,et al.Global genetic variation in the Asian citrus psyllid,Diaphorina citri(Hemiptera:Liviidae)and the endosymbiont Wolbachia:Links between Iran and the USA detected[J].Pest Management Science,2014,70:1033-1040.
[20]DE LEON J H,SETAMOU M,GASTAMINZA G A,et al.Two separate introductions of Asian citrus psyllid populations found in the American continents[J].Annals of the Entomological Society of America,2011,104:1392-1398.
[21]MIYA M,TAKESHIMA H,ENDO H,et al.Major patterns of higher teleostean phylogenies:a new perspective based on100complete mitochondrial DNA sequences[J].Molecular Phylogenetics and Evolution,2003,26:121-138.
[22]MERANER A,BRANDSTTTER A,THALER R,et al.Molecular phylogeny and population structure of the codling moth(Cydia pomonella)in Central Europe:I.Ancient clade splitting revealed by mitochondrial haplotype markers[J].Molecular Phylogenetics and Evolution,2008,48:825-837.
[23]HALL T A.BioEdit:a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT[J].Nucleic Acids Symposium Series,1999,41:95-98.
[24]KATOH K,Kuma K,Toh H,et al.MAFFT version 5:improvement in accuracy of multiple sequence alignment[J].Nucleic Acids Research,2005,33:511-518.
[25]LIBRADO P,ROZAS J.DnaSP v5:a software for comprehensive analysis of DNA polymorphism data[J].Bioinformatics,2009,25:1451-1452.
[26]ROGERS A R,HARPENDING H.Population growth makes waves in the distribution of pairwise genetic differences[J].Molecular Biology and Evolution,1992,9:552-569.
[27]RAY N,Currat M,Excoffier L.Intra-deme molecular diversity in spatially expanding populations[J].Molecular Biology and Evolution,2003,20:76-86.
[28]EXCOFFIER L.Patterns of DNA sequence diversity and genetic structure after a range expansion:lessons from the infinite island model[J].Molecular Ecology,2004,13:853-864.
[29]柯冲,陈辉,林先沾.柑橘黄龙病研究工作小结[J].福建果树,1980(3):1-4.
[2]邓明学.以控制木虱为重点的柑桔黄龙病综合防治理论的形成过程、依据和技术要点[J].中国农学通报,2009,25(23):358-363.
[3]DA GRAA J V.Biology,history,and world status of Huanglongbing[C]∥Taller International sobre Huanglongbing de los cítricos(Candidatus Liberibacter spp.)yel psílido asiático de los cítricos(Diaphorina citri),7-9May 2008,Hermosillo,Sonora,Mexico.
[4]HALBERT S E,MANJUNATH K L.Asian citrus psyllids(Sternorrhyncha:Psyllidae)and greening disease of citrus:a literature review and assessment of risk in Florida[J].Florida Entomologist,2004,87:330-353.
[5]HOLLIS D.A new citrus-feeding psyllid from the Comoro Islands,with a review of the Diaphorina amoena species group(Homoptera)[J].Systematic Entomology,1987,12:47-61.
[6]WATERHOUSE D F.Biological control of insect pests:Southeast Asian prospects[M].ACIAR Monograph Series No.51.Canberra:Australian Centre for International Agricultural Research.1998.
[7]HALBERT S E,NU'EZ C A.Distribution of the Asian citrus psyllid Diaphorina citri Kuwayama(Rhynchota:Psyllidae)in the Caribbean basin[J].Florida Entomologist,2004,87:401-402.
[8]LUO Y F,AGNARSSON I.Global mtDNA genetic structure and hypothesized invasion history of a major pest of citrus,Diaphorina citri(Hemiptera:Liviidae)[J].Ecology and Evolution,2018,8:257-265.
[9]ZHAO X.Occurrence of citrus yellow shoot(greening)disease in Guangxi and southern Sichuan:a brief review[C]∥The FAO-UNDP Project Coordinator in Fuzhou.Regional workshop on citrus greening huanglongbing disease,6-12December 1987,Fuzhou,China.
[10]YANG Y.Effects on light,temperature and humidity on the development,reproduction and survival of citrus psylla[J].Acta Ecologica Sinica,1989,9:348-354.
[11]XU C,XIA Y,KE C.Study on the biology and control of citrus psylla[J].Acta Phytophylacica Sinica,1994,21:53-56.
[12]WANG Z.Distribution and transmission of citrus psylla in Guizhou Province[J].Tillage and Cultivation,2002,22(4):62-63.
[13]CARMICHAEL L E,KRIZAN J,NAGY J A,et al.Historical and ecological determinants of genetic structure in arctic canids[J].Molecular Ecology,2007,16:3466-3483.
[14]PINHO C,HARRIS D J,FERRAND N.Comparing patterns of nuclear and mitochondrial divergence in a cryptic species complex:the case of Iberian and North African wall lizards(Podarcis,Lacertidae)[J].Botanical Journal of the Linnean Society,2007,91:121-133.
[15]JOURDIE V,ALVAREZ N,MOLINA-OCHOA J,et al.Population genetic structure of two primary parasitoids of Spodoptera frugiperda(Lepidoptera),Chelonus insularis and Campoletis sonorensis(Hymenoptera):to what extent is the host plant important?[J].Molecular Ecology,2010,19:2168-2179.
[16]REMAIS J V,XIAO N,AKULLIAN A.Genetic assignment methods for gaining insight into the management of infectious disease by understanding pathogen,vector,and host movement[J/OL].PLoS Pathogens,2011,7:4.
[17]BOYKIN L M,DE BARRO P,HALL D G,et al.Overview of worldwide diversity of Diaphorina citri Kuwayama mitochondrial cytochrome oxidase I haplotypes:two Old World lineages and a New World invasion[J].Bulletin of Entomological Research,2012,102:573-582.
[18]GUIDOLIN A S,FRESIA P,CNSOLI F L.The genetic structure of an invasive pest,the Asian citrus psyllid Diaphorina citri(Hemiptera:Liviidae)[J/OL].PLoS ONE,2014,9:e115749.
[19]LASHKARI M,MANZARI S,SAHRAGARD A,et al.Global genetic variation in the Asian citrus psyllid,Diaphorina citri(Hemiptera:Liviidae)and the endosymbiont Wolbachia:Links between Iran and the USA detected[J].Pest Management Science,2014,70:1033-1040.
[20]DE LEON J H,SETAMOU M,GASTAMINZA G A,et al.Two separate introductions of Asian citrus psyllid populations found in the American continents[J].Annals of the Entomological Society of America,2011,104:1392-1398.
[21]MIYA M,TAKESHIMA H,ENDO H,et al.Major patterns of higher teleostean phylogenies:a new perspective based on100complete mitochondrial DNA sequences[J].Molecular Phylogenetics and Evolution,2003,26:121-138.
[22]MERANER A,BRANDSTTTER A,THALER R,et al.Molecular phylogeny and population structure of the codling moth(Cydia pomonella)in Central Europe:I.Ancient clade splitting revealed by mitochondrial haplotype markers[J].Molecular Phylogenetics and Evolution,2008,48:825-837.
[23]HALL T A.BioEdit:a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT[J].Nucleic Acids Symposium Series,1999,41:95-98.
[24]KATOH K,Kuma K,Toh H,et al.MAFFT version 5:improvement in accuracy of multiple sequence alignment[J].Nucleic Acids Research,2005,33:511-518.
[25]LIBRADO P,ROZAS J.DnaSP v5:a software for comprehensive analysis of DNA polymorphism data[J].Bioinformatics,2009,25:1451-1452.
[26]ROGERS A R,HARPENDING H.Population growth makes waves in the distribution of pairwise genetic differences[J].Molecular Biology and Evolution,1992,9:552-569.
[27]RAY N,Currat M,Excoffier L.Intra-deme molecular diversity in spatially expanding populations[J].Molecular Biology and Evolution,2003,20:76-86.
[28]EXCOFFIER L.Patterns of DNA sequence diversity and genetic structure after a range expansion:lessons from the infinite island model[J].Molecular Ecology,2004,13:853-864.
[29]柯冲,陈辉,林先沾.柑橘黄龙病研究工作小结[J].福建果树,1980(3):1-4.