丝素蛋白及其复合材料的研究进展
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摘要
丝素蛋白是天然高分子材料,具有很好的生物相容性、生物降解性等优良的性能,在医药、食品和美容等领域具有很好的发展前景。本文阐述了丝素蛋白及其分别与天然生物蛋白、无机物、合成聚合物、碳纳米管和氧化石墨烯进行复合的制备技术、材料结构、性能以及研究发展趋势,介绍了丝素蛋白及其复合材料的不同制备机制,分析了制备方法与结构和材料性能之间的关系及不同材料间的相互作用机理,总结了其在组织工程、药物释放和抗凝血性等方面的应用,并提出丝素蛋白复合材料实现规模化生产、发展智能材料以及从基因层面对丝素蛋白进行重组改性方面的未来发展趋势。
Silk fibroin is a natural polymer with biocompatibility,biodegradability and other excellent properties,which is a promising material in the fields of medicine,food and cosmetics,etc.In this work,the preparation techniques,material structure,properties and development trend for silk fibroin and its composites with natural biological protein,inorganic material,synthetic polymer,carbon nanotube and graphene oxide were introduced,respectively.The preparation principles of materials were explained,with an emphasis on the relationship between preparation methods and properties.The interaction mechanism between individual composites was analyzed,and the application on tissue engineering,drug application,anticoagulant materials and other aspects were also summarized.According to the key factors which restrict the research and development of silk fibroin and its composites,the promising future development ways were proposed,for example,realizing the scale production,developing the intelligent materials and restructuring modification of silk fibroin from the gene aspect.
Silk fibroin is a natural polymer with biocompatibility,biodegradability and other excellent properties,which is a promising material in the fields of medicine,food and cosmetics,etc.In this work,the preparation techniques,material structure,properties and development trend for silk fibroin and its composites with natural biological protein,inorganic material,synthetic polymer,carbon nanotube and graphene oxide were introduced,respectively.The preparation principles of materials were explained,with an emphasis on the relationship between preparation methods and properties.The interaction mechanism between individual composites was analyzed,and the application on tissue engineering,drug application,anticoagulant materials and other aspects were also summarized.According to the key factors which restrict the research and development of silk fibroin and its composites,the promising future development ways were proposed,for example,realizing the scale production,developing the intelligent materials and restructuring modification of silk fibroin from the gene aspect.
引文
[1]张扬,李明忠.改善再生丝素蛋白材料力学性能的研究进展[J].现代丝绸科学与技术,2014,29(6):236-240.ZHANG Y,LI M Z.Research progress in improving mechanical properties of regenerated silk fibroin material[J].Modern Silk Science&Technology,2014,29(6):236-240.
[2]黄泉,杨吉成,缪竞诚.丝素蛋白生物相容性评价进展[J].国际生物医学工程杂志,2007,30(3):163-166.HUANG Q,YANG J C,LIAO J C.Progress in biocompatibility evaluation of silk fibroin[J].International Journal of Biomedical Engineering,2007,30(3):163-166.
[3]KAPLAN D L,MC G K.Protein based materials[M].Boston:Birkhauser,1998:103-131.
[4]刘琼,陈忠敏,陈枭,等.纳米蚕丝素蛋白/PEG复合材料的抗菌性与药物缓释效果[J].材料导报,2014,28(2):55-59.LIU Q,CHEN Z M,CHEN X,et al.Antibacterial properties and delayed release effect of nano-silk fibroin peptide polyethylene glycol graft polymer[J].Materials Review,2014,28(2):55-59.
[5]许云辉,李继丰,王云霞,等.丝素酰胺改性棉织物的结构及性能[J].纺织学报,2017,38(2):134-140.XU Y H,LI J F,WANG Y X,et al.Structure and properties of cotton fabric by amide modification of silk fibroin[J].Journal of Textile Research,2017,38(2):134-140.
[6]DHYANI V,SINGH N.Controlling the cell adhesion property of silk films by graft polymerization[J].Appl Mater Interfaces,2014,6(7):5005-5011.
[7]杨华,聂涛,蔡辉,等.聚乙二醇缩水甘油醚改性丝素蛋白研究[J].化工科技,2013,21(5):4-8.YANG H,NIE T,CAI H,et al.Modification of silk fibroin with polyethylene glycol diglycidyl[J].Science&Technology in Chemical Industry,2013,21(5):4-8.
[8]KASOJU N,BORA U.Silk fibroin in tissue engineering[J].Advances Healthcare Materials,2012,1:393-412.
[9]肖阳,杨蓉,李庆荣,等.丝素蛋白在生物医药工程中的应用[J].广东蚕业,2016,50(2):24-29.XIAO Y,YANG R,LI Q R,et al.Application of silk fibroin in biomedical engineering[J].Guangdong Silk,2016,50(2):24-29.
[10]潘岳林,杨明英,邓连霞,等.丝素在组织工程领域中的研究热点[J].蚕桑通报,2016,47(2):15-17.PAN Y L,YANG M Y,DENG L X,et al.Silk fibroin for tissue engineering[J].Bulletin of Sericulture,2016,47(2):15-17.
[11]孙越宜,俞纪维,支金丝,等.丝素蛋白对重金属离子吸附性能的研究[J].蚕桑通报,2015,46(1):37-40.SUN Y Y,YU J W,ZHI J S,et al.Study on the adsorption of heavy metal ions by silk fibroin[J].Bulletin of Sericulture,2015,46(1):37-40.
[12]张凯,谢孔良.水不溶性丝素杂化膜的制备及其金属离子吸附性能研究[J].印染助剂,2016,33(4):18-23.ZHANG K,XIE K L.Preparation and metal ion adsorption properties of water-insoluble silk fibroin hybrid membrane[J].Textile Auxiliaries,2016,33(4):18-23.
[13]LUO K Y,SHAO Z Z.A novel regenerated silk fibroin-based hydrogels with magnetic and catalytic activities[J].Chinese Journal of Polymer Science,2017,35(4):515-523.
[14]HAYASHI C Y,LEWIS R V.Evidence from flagelliform silk cDNA for the structural basis of elastic city and modular nature of spider silk[J].Journal of Molecular Biology,1998,275:773-784.
[15]CRISTIAN C R,CLIFFORF B,HA S W,et al.Conformational changes induced in bombyxmori silk fibroin bycyclodextrin inclusion complexation[J].Macromolecules,2005,38(13):5640-5646.
[16]VALLUZZI R,GIDO S,ZHANG W,et al.Trigonal crystal structure of bombyx mori silk incorporating threefold helical chain conformation found at the air-water interface[J].Macromolecules,1996,29(95):8606-8614.
[17]FURUHATA K I,OKADA A,CHEN Y,et al.Dissolution of silk fibroin in lithium halide/organic amide solvent systems[J].Polymer,1994,63(4):315-322.
[18]于海洋,王昉,刘其春,等.新型丝素蛋白膜的结构和热分解动力学机理[J].物理化学学报,2017,33(2):344-355.YU H Y,WANG F,LIU Q C,et al.Structure and kinetics of thermal decomposition mechanism of novel silk fibroin films[J].Acta Physico-Chimica Sinica,2017,33(2):344-355.
[19]SIONKOWSKA A,LEWANDOWSKA K,MICHALSKA M,et al.Characterization of silk fibroin 3Dcomposites modified by collagen[J].Journal of Molecular Liquids,2016,215:323-327.
[20]LV X G,LI Z,CHEN S Y,et al.Structural and functional evaluation of oxygenating keratin/silk fibroin scaffold and initial assessment of their potential for urethral tissue engineering[J].Biomaterials,2016,84(4):99-110.
[21]NUMATA K,YAMAZAKI S,KATASHIMA T,et al.Silk-petin hydrogel with superior mechanical properties,biodegrad-ability,and biocompatibility[J].Macromolecular Bioscience,2014,14(6):799-806.
[22]LEE H,JANG C H,KIM G H.A polycaprolactone/silk-fibroin nanfibrous composite combined with human umbilical cord serum for subacute tympanic mambrane perforation;an in vitro and vivo study[J].Journal of Materials Chemistry B,2014,2(18):2703-2713.
[23]ASAKURA T,OKUSHITA K,MIKE P.Analysis of the structure of bombyx mori silk fibroin by NMR[J].Macromolecules,2015,48(8):2345-2357.
[24]余劭婷,秦金桥,关国平,等.丝素纤维表面改性提高细胞粘附性能[J].生物医学工程学进展,2016,37(3):144-149.YU S T,QIN J Q,GUAN G P,et al.Surface modification of silk fibroin fiber for improving cell adhesion[J].Progress in Biomedical Engineering,2016,37(3):144-149.
[25]SAGNELLA A,PISTONE A,BONETTI S,et al.Effect of different fabrication methods on the chemo-physical properties of silk fibroin films and on their interaction with neural cells[J].RSC Advances,2016,6(11):9304-9314.
[26]王鹏,左保齐.不同盐/甲酸溶解体系下丝素膜的制备及性能表征[J].丝绸,2016,57(7):18-22.WANG P,ZUO B Q.Preparation of silk fibroin films in different salt/formic acid dissolution system and its property characterization[J].Silk,2016,57(7):18-22.
[27]JIAN W Y,ER Q C,DAVID P,et al.Enhancing the toughness of regenerated silk fibroin film through uniaxial extension[J].Biomacromolecules,2010,11:2890-2895.
[28]TAKAYUKI A,FREDDI G,INNOCENTI R,et al.Biodegradation of bombyxmori silk fibroin fibers and films[J].Journal of Applied Polymer Science,2004,91(4):2383-2390.
[29]朱正华,陆旋,周晓红.钙离子含量对再生丝素蛋白纤维生物降解性的影响[J].蚕业科学,2014,40(1):107-112.ZHU Z H,LU X,ZHOU X H.Impact of calcium ion content on biodegradablity of the regenerated silk fibroin fiber[J].Science of Sericulture,2014,40(1):107-112.
[30]马晓晔,施丽君,周涓,等.聚丙烯酸钠和丝素蛋白自膨胀水凝胶的制备及其释药性能[J].有机化学,2013,33(5):1080-1087.MA X Y,SHI L J,ZHOU J,et al.Preparation of the self-inflating hydrogels based on poly(sodium acrylate)and silk fibroin and their drug releasing properties[J].Chinese Journal of Organic Chemistry,2013,33(5):1080-1087.
[31]ROMERO I S,SCHURR M L,LALLY J V,et al.Enhancing the interface in silk-polypyrrole composites through chemical modification of silk fibroin[J].ACS Appl Mater&Interfaces,2013,5(3):553-564.
[32]YUE X X,ZHANG F,WU H Y,et al.A novel route to prepare dry-spun silk fibers from CaCl2-formic acid solution[J].Materials Letters,2014,128(8):175-178.
[33]WANG J L,YANG Q,MAO C B,et al.Osteogenic differentiation of bone marrow mesenchymal stem cells collagen/silk fibroin bi-template-induced biomimetic bone substitutes[J].Journal of Biomedical Materials Research Part A,2012,100(11):2929.
[34]ZHU H L,WU B W,FENG X X,et al.Preparation and characterization of bioactive mesoporous calcium silicate-silk fibroin composites films[J].Journal of Biomedical Materials Research Part B Applied Biomaterials,2011,98(2):330.
[35]张睿晗,蔡志江.静电纺制备丝素蛋白纳米纤维及其复合纳米纤维的研究进展[J].高分子通报,2013(7):33-39.ZHANG R H,CAI Z J.Advances on fabrication of electrospun silk fibroin nanofibres[J].Chinese Polymer Bulletin,2013(7):33-39.
[36]崔新爱,刘欣,孔德领,等.静电纺胶原/丝素复合微纳米纤维的制备及细胞相容性研究[J].中国生物医学工程学报,2012,31(2):291-299.CUI X A,LIU X,KONG D L,et al.Preparation of collagen/silk fibroin bicomponent micro-nanofibers and its cytocompatibility study[J].Chinese Journal of Biomedical Engineering,2012,31(2):291-299.
[37]LI S,WU H,HU X,et al.Preparation of electrospun PLGA-silk fibroin nanofibers-based nerve conduits and evaluation in vivo[J].Artifcells Blood Substitues Immobilization Biotechol,2012,40(1/2):171-178.
[38]XU H,LIU C,SILBERSCHMIT V V,et al.Behavior of aluminum oxide,intermetallics and voids in Cu-Al wire bonds[J].Acta Materialia,2011,41(5):5611.
[39]NAZAROV R,JIN H J,KAPLAN D L.Porous 3-D scaffolds from regenerated silk fibroin[J].Biomacromolecules,2004,5(3):718-726.
[40]孙凯,李瑞欣,范猛,等.3D打印丝素蛋白/胶原蛋白支架的制备及性能[J].中国组织工程研究,2017,21(2):280-285.SUN K,LI R X,FAN M,et al.Three-dimensional printed silk fibroin/collagen scaffold and its performance[J].Chinese Journal of Tissue Engineering Research,2017,21(2):280-285.
[41]陈慧.羊毛角蛋白丝素膜的制备及性能表征[J].毛纺科技,2016,44(6):46-49.CHEN H.Preparation and properties characterization of wool keratin-silk fibroin membrane[J].Wool Textile Journal,2016,44(6):46-49.
[42]冯超,李喆,吕向国,等.自产氧型人发角蛋白/丝素蛋白复合材料体外制备及生化评估[J].中国组织工程研究,2014,18(52):8480-8486.FENG C,LI Z,LV X G,et al.In vitro preparation and biochemical evaluation of oxygen generative keratin/silk fibroin compound biomaterial[J].Chinese Journal of Tissue Engineering Research,2014,18(52):8480-8486.
[43]马育栋,卢志华.丝素蛋白/壳聚糖复合凝胶材料的制备[J].济宁学院学报,2013,34(6):41-43.MA Y D,LU Z H.Preparation of gel material with silk fibroin and chitosan[J].Journal of Jining University,2013,34(6):41-43.
[44]柳磊,曾曙光,仁力,等.丝素蛋白/壳聚糖三维多孔支架的构建及结构表征[J].中国组织工程研究,2012,16(12):2197-2202.LIU L,ZENG S G,REN L,et al.Preparation and characterization of porous silk fibroin/chitosan scaffolds[J].Chinese Journal of Tissue Engineering Research,2012,16(12):2197-2202.
[45]郑丽,朱平,张林,等.纤维素/丝素蛋白共混膜的制备及性能研究[J].印染助剂,2012,29(6):27-29.ZHENG L,ZHU P,ZHANG L,et al.Study on the preparation and performance of cellulose/silk fibroin composite film[J].Textile Auxiliaries,2012,29(6):27-29.
[46]李娟,何建新.纤维素/丝素复合膜的制备与性能[J].纤维素科学与技,2011,19(3):62-67.LI J,HE J X.Preparation and properties of cellulose-silk compound membrane[J].Journal of Cellulose Science and Technology,2011,19(3):62-67.
[47]白莲村,柯勤飞,黄晨.丝素/胶原纤维针刺支架材料的制备和性能测试[J].产业用纺织品,2016(6):17-20.BAI L C,KE Q F,HUANG C.Preparation and performance test of tissue engineering scaffold materials of silk fibers and collagen fibers with needle-punch technique[J].Technical Textiles,2016(6):17-20.
[48]李曦,刘连利,王莉莉.纳米二氧化硅的研究现状与进展[J].渤海大学学报,2006,27(4):304-308.LI X,LIU L L,WANG L L.Status quo and progress in research nano-SiO2[J].Journal of Bohai University Natural Science Edition,2006,27(4):304-308.
[49]徐水,张胡静,李雯静,等.丝素/纳米SiO2凝胶共混膜的制备及性能测试[J].蚕业科学,2011,37(1):82-87.XU S,ZHANG H J,LI W J,et al.Preparation and property determination of fibroin/nano-SiO2gel blend membrane[J].Science of Sericulture,2011,37(1):82-87.
[50]MIN K,UMAR M,HAEKYO S,et al.Optically transparent,patterned,and flexible electrodes and radio frequency antennas prepared from silk protein and silver nanowire networks[J].RSC Advances,2017,7(1):574-580.
[51]范纯泉,叶晓健.丝素蛋白纳米羟基磷灰石复合材料的生物安全性[J].中国组织工程研究,2016,20(38):5650-5656.FAN C Q,YE X J.Biological safety of silk fibroin/nanohydroxyapatite composites[J].Chinese Journal of Tissue Engineering Research,2016,20(38):5650-5656.
[52]刘浩,褚亚伟,丁涛,等.丝素蛋白/羟基磷灰石支架对成骨诱导脂肪干细胞活性及成骨性能的影响[J].中国组织工程研究,2015,19(45):7224-7229.LIU H,ZHU Y W,DING T,et al.Effect of silk fibroin/hydroxyapatite scaffold on the viability and osteogenic properties of adipose-derived stem cells under osteogenic induction[J].Chinese Journal of Tissue Engineering Research,2015,19(45):7224-7229.
[53]宁旭,庄勇,刘淼,等.丝素蛋白纳米羟基磷灰石复合材料制备及与脊柱的融合[J].中国组织工程研究,2017,21(2):221-226.NING X,ZHUANG Y,LIU M,et al.Preparation of silk fibroin/nano-hydroxypatite composite scaffold and its treatment outcomes in spinal fusion[J].Chinese Journal of Tissue Engineering Research,2017,21(2):221-226.
[54]MI R X,LIU Y X,CHEN X,et al.Structure and properties of various hybrids fabricated by silk nanofibrils and nanohydroxyapatite[J].Nanoscale,2016,8:20096-20102.
[55]李静静,朱海霖,雷彩虹,等.介孔生物玻璃/丝素蛋白复合多孔海绵的结构及止血性能研究[J].功能材料,2017,48(2):2097-2101.LI J J,ZHU H L,LEI C H,et al.Study on structure and hemostatic property of mesoporous bioglass/silk fibroin composite sponge[J].Journal of Functional Materials,2017,48(2):2097-2101.
[56]张静,肖长发,王健,等.丝素蛋白/聚丙烯腈共混膜的制备及性能研究[J].天津工业大学学报,2011,30(6):5-8.ZHANG J,XIAO C F,WANG J,et al.Study on preparation and properties of SF/PAN blend membranes[J].Journal of Tianjin Polytechnic University,2011,30(6):5-8.
[57]钱巧芬,张珊珊,侯静,等.乙二醇/丝素蛋白共混膜的研究[J].丝绸,2013,50(9):1-6.QIAN Q F,ZHANG S S,HOU J,et al.Study on ethylene glycol/silk fibroin blend membrane[J].Silk Monthly,2013,50(9):1-6.
[58]SUZUKI S,REBBACA A,TRAIAN V,et al.Treatment of silk fibroin with poly(ethylene glycol)for the enhancement of corneal epithelial cell growth[J].Journal of Functional Biomaterials,2015,6(2):345-366.
[59]王利君,熊杰,骆菁菁,等.聚乳酸-聚己内酯/丝素蛋白三元复合纳米纤维膜支架的结构与性能[J].纺织学报,2017,38(5):8-13.WANG L J,XIONG J,LUO J J,et al.Structure and properties of polylactic acid-polycaprolactone/silk fibroin composite nanofibrous scaffolds[J].Journal of Textile Research,2014,38(5):8-13.
[60]肖红伟,熊杰,李妮,等.聚乳酸-乙醇酸共聚物/丝素共混纳米纤维多孔膜的制备及性能[J].高分子材料科学与工程,2011,27(12):142-145.XIAO H W,XIONG J,LI N,et al.Preparation and properties of poly(lactide-co-glycolide)/silk blend nanofibrous porous membrane[J].Polymer Materials Science&Engineering,2011,27(12):142-145.
[61]PAN H,ZHANG Y P,HANG Y C,et al.Significantly reinforced composite fibers electrospun from silk finroin/carbon nanotube aqueous solutions[J].Biomacromolacules,2012,13:2859-2867.
[62]潘彩霞.丝素/碳纳米管及丝素/EDC复合膜的制备及性能研究[D].杭州:浙江大学,2016.PAN C X.Preparation and properties of SF/MWCNT and SF/EDC composites films[D].Hangzhou:Zhejiang University,2016.
[63]PAN H,ZHANG Y,HANG Y,et al.Significantly reinforced composite fibers electrospun from silk fibroin/carbon nanotube aqueous solutions[J].Biomacromolecules,2012,13(9):2859-2876.
[64]杨珊珊,沙金,赵海利,等.氧化石墨烯掺杂丝素蛋白复合材料的结晶和力学特性[J].高分子材料科学与工程,2016,32(1):46-51.YANG S S,SHA J,ZHAO H L,et al.Crystallinity and mechanical property of silk fibroin/graphene oxide composites[J].Polymer Materials Science&Engineering,2016,32(1):46-51.
[65]方洁羽,李延报,李怀栋.丝素-氧化石墨烯-甘油复合膜的制备及性能[J].功能高分子学报,2015,28(1):73-78.FANG J Y,LI Y B,LI H D.Preparation and properties of silk fibroin-graphene oxide-glycerin composite films[J].Journal of Functional Polymers,2015,28(1):73-78.
[66]ZHANG C,ZHANG Y P,LUO J,et al.Microstructural evolution of regenerated silk fibroin/graphene oxide hybrid fibers under tensile deformation[J].RSC Advances,2017,7(6):3108-3116.
[67]郭文君,吴建兵.丝素蛋白材料在药物控释中的应用[J].轻工科技,2017(5):23-24.GUO W J,WU J B.Application of silk fibroin material in controlled release of drugs[J].Light Industry Science and Technology,2017(5):23-24.
[68]YANG L,ZHENG Z Z,QIAN C,et al.Curcumin-functionalized silk biomaterials for anti-aging utility[J].Journal of Colloid and Interface Science,2017,496:66-77.
[69]张桂媛.丝素蛋白止血材料的制备及其性能研究[D].苏州:苏州大学,2015.ZHANG G Y.Preparation and properties of silk fibroin hemostatic material[D].Suzhou:Soochow University,2015.
[70]姚康德,尹玉姬.组织工程相关生物材料[M].北京:化学工业出版社,2003:100-120.YAO K D,YIN Y J.Biomaterials for tissue engineering[M].Beijing:Chemical Industry Press,2003:100-120.
[71]郭志豪,田林强,刘槃,等.丝素蛋白/介孔生物玻璃陶瓷复合材料修复颅骨缺损[J].中国组织工程研究,2017,21(2):192-196.GUO Z H,TIAN L Q,LIU P,et al.Repair of skull defects with silk fibroin/mesoporous glass ceramic composites[J].Chinese Journal of Tissue Engineering Research,2017,21(2):192-196.
[72]李明,莫茂松.基于石墨烯/丝素蛋白的骨组织工程三维多孔支架制备方法:CN201610097176.2[P].2016-06-15.LI M,MO M S.Preparation of three dimensional porous scaffold for bone tissue engineering based on graphene/fibroin:CN201610097176.2[P].2016-06-15.
[73]胡沐黎.丝素蛋白-磷酸钙类复合材料在椎体强化以及骨软骨修复中的应用研究[D].苏州:苏州大学,2016.HU M L.Research on the application of silk fibroin calcium phosphate composite in vertebral augmentation and osteochondral repair[D].Suzhou:Soochow University,2016.
[74]ZHANG Y Q.Natural silk fibroin as a support for enzymeimmobilization[J].Biotechnology Advances,1998,16(5):961-971.
[75]XU J,TANG T,ZHANG K,et al.Electroenzymatic catalyzed oxidation of bisphenol-a using HRP immobilized on magnetic silk fibroin nanoparticles[J].Process Biochemistry,2011,46(5):1160-1165.
[76]DELEZUK J,PAVINATTO A,MORAES M,et al.Silk fibroin organization induced by chitosan in layer-by-layer films:application as a matrix in a biosensor[J].Carbohydrate Polymers,2017,155:146-151.
[77]孙越宜,唐科梦,杨明英,等.丝素蛋白吸附金属离子的性能及其机理的研究[J].蚕桑通报,2016,47(2):26-31.SUN Y Y,TANG K M,YANG M Y,et al.Study of the adsorption and the mechanism of metal ions by fibroin[J].Bulletin of Sericulture,2016,47(2):26-31.
[78]李继丰,杨震,彭凤霞,等.羧基化棉织物的丝素蛋白接枝改性[J].印染,2015(7):1-6.LI J F,YANG Z,PENG F X,et al.Modification of carboxylated cotton fabric with silk fibroin[J].Shtexcloud,2015(7):1-6.
[79]李陈梅,熊佳庆,张德锁,等.丝素纳米银的制备及其对棉织物的改性实验研究[J].纺织导报,2014(1):102-105.LI C M,XIONG J Q,ZHANG D S,et al.Preparation of silk fibroin stabilized silver nanoparticles and their application for modification of cotton fabric[J].China Textile Leader,2014(1):102-105.
[80]赵林,林小贞,赖宏波,等.丝素蛋白/TiO2复合催化剂的制备及其可见光催化性能[J].分子催化,2014,28(3):275-281.ZHAO L,LIN X Z,LAI H B,et al.Preparation and visible light photocatalytic activity of silk fibroin/TiO2 nanocomposite[J].Journal of Molecular Catalysis,2014,28(3):275-281.
[81]MARELLI B,BRENCKLE M A,KAPLAN D L,et al.Silk fibroin as edible coating for perishable food preservation[J].Scientific Reports,2016,6:25263.
[82]屠洁,刘冠卉,燕薇.4种常用稳定剂和丝素蛋白对搅拌型酸奶黏度和保水性的影响[J].食品科学,2012,33(21):136-140.TU J,LIU G H,YAN W.Effects of four common stabilizers combined with silk fibroin on viscosity and water-holding capacity of stirred yogurt[J].Food Science,2012,33(21):136-140.
[83]赵应征,鲁翠涛,肖健,等.一种肌肤护理用丝素蛋白水凝胶及其制备方法:CN106420371A[P].2017-02-22.ZHAO Y Z,LU C T,XIAO J,et al.Preparation of skin care silk fibroin hydrogel:CN106420371A[P].2017-02-22.
[84]曹丽楠,徐保来.医用丝素蛋白皮肤再生膜的生物相容性评价[J].中国组织工程研究,2016,20(25):3653-3658.CAO L N,XU B L.Biocompatibility evaluation for skin reproducing membrane of medical fibroin[J].Chinese Journal of Tissue Engineering Research,2016,20(25):3653-3658.
[2]黄泉,杨吉成,缪竞诚.丝素蛋白生物相容性评价进展[J].国际生物医学工程杂志,2007,30(3):163-166.HUANG Q,YANG J C,LIAO J C.Progress in biocompatibility evaluation of silk fibroin[J].International Journal of Biomedical Engineering,2007,30(3):163-166.
[3]KAPLAN D L,MC G K.Protein based materials[M].Boston:Birkhauser,1998:103-131.
[4]刘琼,陈忠敏,陈枭,等.纳米蚕丝素蛋白/PEG复合材料的抗菌性与药物缓释效果[J].材料导报,2014,28(2):55-59.LIU Q,CHEN Z M,CHEN X,et al.Antibacterial properties and delayed release effect of nano-silk fibroin peptide polyethylene glycol graft polymer[J].Materials Review,2014,28(2):55-59.
[5]许云辉,李继丰,王云霞,等.丝素酰胺改性棉织物的结构及性能[J].纺织学报,2017,38(2):134-140.XU Y H,LI J F,WANG Y X,et al.Structure and properties of cotton fabric by amide modification of silk fibroin[J].Journal of Textile Research,2017,38(2):134-140.
[6]DHYANI V,SINGH N.Controlling the cell adhesion property of silk films by graft polymerization[J].Appl Mater Interfaces,2014,6(7):5005-5011.
[7]杨华,聂涛,蔡辉,等.聚乙二醇缩水甘油醚改性丝素蛋白研究[J].化工科技,2013,21(5):4-8.YANG H,NIE T,CAI H,et al.Modification of silk fibroin with polyethylene glycol diglycidyl[J].Science&Technology in Chemical Industry,2013,21(5):4-8.
[8]KASOJU N,BORA U.Silk fibroin in tissue engineering[J].Advances Healthcare Materials,2012,1:393-412.
[9]肖阳,杨蓉,李庆荣,等.丝素蛋白在生物医药工程中的应用[J].广东蚕业,2016,50(2):24-29.XIAO Y,YANG R,LI Q R,et al.Application of silk fibroin in biomedical engineering[J].Guangdong Silk,2016,50(2):24-29.
[10]潘岳林,杨明英,邓连霞,等.丝素在组织工程领域中的研究热点[J].蚕桑通报,2016,47(2):15-17.PAN Y L,YANG M Y,DENG L X,et al.Silk fibroin for tissue engineering[J].Bulletin of Sericulture,2016,47(2):15-17.
[11]孙越宜,俞纪维,支金丝,等.丝素蛋白对重金属离子吸附性能的研究[J].蚕桑通报,2015,46(1):37-40.SUN Y Y,YU J W,ZHI J S,et al.Study on the adsorption of heavy metal ions by silk fibroin[J].Bulletin of Sericulture,2015,46(1):37-40.
[12]张凯,谢孔良.水不溶性丝素杂化膜的制备及其金属离子吸附性能研究[J].印染助剂,2016,33(4):18-23.ZHANG K,XIE K L.Preparation and metal ion adsorption properties of water-insoluble silk fibroin hybrid membrane[J].Textile Auxiliaries,2016,33(4):18-23.
[13]LUO K Y,SHAO Z Z.A novel regenerated silk fibroin-based hydrogels with magnetic and catalytic activities[J].Chinese Journal of Polymer Science,2017,35(4):515-523.
[14]HAYASHI C Y,LEWIS R V.Evidence from flagelliform silk cDNA for the structural basis of elastic city and modular nature of spider silk[J].Journal of Molecular Biology,1998,275:773-784.
[15]CRISTIAN C R,CLIFFORF B,HA S W,et al.Conformational changes induced in bombyxmori silk fibroin bycyclodextrin inclusion complexation[J].Macromolecules,2005,38(13):5640-5646.
[16]VALLUZZI R,GIDO S,ZHANG W,et al.Trigonal crystal structure of bombyx mori silk incorporating threefold helical chain conformation found at the air-water interface[J].Macromolecules,1996,29(95):8606-8614.
[17]FURUHATA K I,OKADA A,CHEN Y,et al.Dissolution of silk fibroin in lithium halide/organic amide solvent systems[J].Polymer,1994,63(4):315-322.
[18]于海洋,王昉,刘其春,等.新型丝素蛋白膜的结构和热分解动力学机理[J].物理化学学报,2017,33(2):344-355.YU H Y,WANG F,LIU Q C,et al.Structure and kinetics of thermal decomposition mechanism of novel silk fibroin films[J].Acta Physico-Chimica Sinica,2017,33(2):344-355.
[19]SIONKOWSKA A,LEWANDOWSKA K,MICHALSKA M,et al.Characterization of silk fibroin 3Dcomposites modified by collagen[J].Journal of Molecular Liquids,2016,215:323-327.
[20]LV X G,LI Z,CHEN S Y,et al.Structural and functional evaluation of oxygenating keratin/silk fibroin scaffold and initial assessment of their potential for urethral tissue engineering[J].Biomaterials,2016,84(4):99-110.
[21]NUMATA K,YAMAZAKI S,KATASHIMA T,et al.Silk-petin hydrogel with superior mechanical properties,biodegrad-ability,and biocompatibility[J].Macromolecular Bioscience,2014,14(6):799-806.
[22]LEE H,JANG C H,KIM G H.A polycaprolactone/silk-fibroin nanfibrous composite combined with human umbilical cord serum for subacute tympanic mambrane perforation;an in vitro and vivo study[J].Journal of Materials Chemistry B,2014,2(18):2703-2713.
[23]ASAKURA T,OKUSHITA K,MIKE P.Analysis of the structure of bombyx mori silk fibroin by NMR[J].Macromolecules,2015,48(8):2345-2357.
[24]余劭婷,秦金桥,关国平,等.丝素纤维表面改性提高细胞粘附性能[J].生物医学工程学进展,2016,37(3):144-149.YU S T,QIN J Q,GUAN G P,et al.Surface modification of silk fibroin fiber for improving cell adhesion[J].Progress in Biomedical Engineering,2016,37(3):144-149.
[25]SAGNELLA A,PISTONE A,BONETTI S,et al.Effect of different fabrication methods on the chemo-physical properties of silk fibroin films and on their interaction with neural cells[J].RSC Advances,2016,6(11):9304-9314.
[26]王鹏,左保齐.不同盐/甲酸溶解体系下丝素膜的制备及性能表征[J].丝绸,2016,57(7):18-22.WANG P,ZUO B Q.Preparation of silk fibroin films in different salt/formic acid dissolution system and its property characterization[J].Silk,2016,57(7):18-22.
[27]JIAN W Y,ER Q C,DAVID P,et al.Enhancing the toughness of regenerated silk fibroin film through uniaxial extension[J].Biomacromolecules,2010,11:2890-2895.
[28]TAKAYUKI A,FREDDI G,INNOCENTI R,et al.Biodegradation of bombyxmori silk fibroin fibers and films[J].Journal of Applied Polymer Science,2004,91(4):2383-2390.
[29]朱正华,陆旋,周晓红.钙离子含量对再生丝素蛋白纤维生物降解性的影响[J].蚕业科学,2014,40(1):107-112.ZHU Z H,LU X,ZHOU X H.Impact of calcium ion content on biodegradablity of the regenerated silk fibroin fiber[J].Science of Sericulture,2014,40(1):107-112.
[30]马晓晔,施丽君,周涓,等.聚丙烯酸钠和丝素蛋白自膨胀水凝胶的制备及其释药性能[J].有机化学,2013,33(5):1080-1087.MA X Y,SHI L J,ZHOU J,et al.Preparation of the self-inflating hydrogels based on poly(sodium acrylate)and silk fibroin and their drug releasing properties[J].Chinese Journal of Organic Chemistry,2013,33(5):1080-1087.
[31]ROMERO I S,SCHURR M L,LALLY J V,et al.Enhancing the interface in silk-polypyrrole composites through chemical modification of silk fibroin[J].ACS Appl Mater&Interfaces,2013,5(3):553-564.
[32]YUE X X,ZHANG F,WU H Y,et al.A novel route to prepare dry-spun silk fibers from CaCl2-formic acid solution[J].Materials Letters,2014,128(8):175-178.
[33]WANG J L,YANG Q,MAO C B,et al.Osteogenic differentiation of bone marrow mesenchymal stem cells collagen/silk fibroin bi-template-induced biomimetic bone substitutes[J].Journal of Biomedical Materials Research Part A,2012,100(11):2929.
[34]ZHU H L,WU B W,FENG X X,et al.Preparation and characterization of bioactive mesoporous calcium silicate-silk fibroin composites films[J].Journal of Biomedical Materials Research Part B Applied Biomaterials,2011,98(2):330.
[35]张睿晗,蔡志江.静电纺制备丝素蛋白纳米纤维及其复合纳米纤维的研究进展[J].高分子通报,2013(7):33-39.ZHANG R H,CAI Z J.Advances on fabrication of electrospun silk fibroin nanofibres[J].Chinese Polymer Bulletin,2013(7):33-39.
[36]崔新爱,刘欣,孔德领,等.静电纺胶原/丝素复合微纳米纤维的制备及细胞相容性研究[J].中国生物医学工程学报,2012,31(2):291-299.CUI X A,LIU X,KONG D L,et al.Preparation of collagen/silk fibroin bicomponent micro-nanofibers and its cytocompatibility study[J].Chinese Journal of Biomedical Engineering,2012,31(2):291-299.
[37]LI S,WU H,HU X,et al.Preparation of electrospun PLGA-silk fibroin nanofibers-based nerve conduits and evaluation in vivo[J].Artifcells Blood Substitues Immobilization Biotechol,2012,40(1/2):171-178.
[38]XU H,LIU C,SILBERSCHMIT V V,et al.Behavior of aluminum oxide,intermetallics and voids in Cu-Al wire bonds[J].Acta Materialia,2011,41(5):5611.
[39]NAZAROV R,JIN H J,KAPLAN D L.Porous 3-D scaffolds from regenerated silk fibroin[J].Biomacromolecules,2004,5(3):718-726.
[40]孙凯,李瑞欣,范猛,等.3D打印丝素蛋白/胶原蛋白支架的制备及性能[J].中国组织工程研究,2017,21(2):280-285.SUN K,LI R X,FAN M,et al.Three-dimensional printed silk fibroin/collagen scaffold and its performance[J].Chinese Journal of Tissue Engineering Research,2017,21(2):280-285.
[41]陈慧.羊毛角蛋白丝素膜的制备及性能表征[J].毛纺科技,2016,44(6):46-49.CHEN H.Preparation and properties characterization of wool keratin-silk fibroin membrane[J].Wool Textile Journal,2016,44(6):46-49.
[42]冯超,李喆,吕向国,等.自产氧型人发角蛋白/丝素蛋白复合材料体外制备及生化评估[J].中国组织工程研究,2014,18(52):8480-8486.FENG C,LI Z,LV X G,et al.In vitro preparation and biochemical evaluation of oxygen generative keratin/silk fibroin compound biomaterial[J].Chinese Journal of Tissue Engineering Research,2014,18(52):8480-8486.
[43]马育栋,卢志华.丝素蛋白/壳聚糖复合凝胶材料的制备[J].济宁学院学报,2013,34(6):41-43.MA Y D,LU Z H.Preparation of gel material with silk fibroin and chitosan[J].Journal of Jining University,2013,34(6):41-43.
[44]柳磊,曾曙光,仁力,等.丝素蛋白/壳聚糖三维多孔支架的构建及结构表征[J].中国组织工程研究,2012,16(12):2197-2202.LIU L,ZENG S G,REN L,et al.Preparation and characterization of porous silk fibroin/chitosan scaffolds[J].Chinese Journal of Tissue Engineering Research,2012,16(12):2197-2202.
[45]郑丽,朱平,张林,等.纤维素/丝素蛋白共混膜的制备及性能研究[J].印染助剂,2012,29(6):27-29.ZHENG L,ZHU P,ZHANG L,et al.Study on the preparation and performance of cellulose/silk fibroin composite film[J].Textile Auxiliaries,2012,29(6):27-29.
[46]李娟,何建新.纤维素/丝素复合膜的制备与性能[J].纤维素科学与技,2011,19(3):62-67.LI J,HE J X.Preparation and properties of cellulose-silk compound membrane[J].Journal of Cellulose Science and Technology,2011,19(3):62-67.
[47]白莲村,柯勤飞,黄晨.丝素/胶原纤维针刺支架材料的制备和性能测试[J].产业用纺织品,2016(6):17-20.BAI L C,KE Q F,HUANG C.Preparation and performance test of tissue engineering scaffold materials of silk fibers and collagen fibers with needle-punch technique[J].Technical Textiles,2016(6):17-20.
[48]李曦,刘连利,王莉莉.纳米二氧化硅的研究现状与进展[J].渤海大学学报,2006,27(4):304-308.LI X,LIU L L,WANG L L.Status quo and progress in research nano-SiO2[J].Journal of Bohai University Natural Science Edition,2006,27(4):304-308.
[49]徐水,张胡静,李雯静,等.丝素/纳米SiO2凝胶共混膜的制备及性能测试[J].蚕业科学,2011,37(1):82-87.XU S,ZHANG H J,LI W J,et al.Preparation and property determination of fibroin/nano-SiO2gel blend membrane[J].Science of Sericulture,2011,37(1):82-87.
[50]MIN K,UMAR M,HAEKYO S,et al.Optically transparent,patterned,and flexible electrodes and radio frequency antennas prepared from silk protein and silver nanowire networks[J].RSC Advances,2017,7(1):574-580.
[51]范纯泉,叶晓健.丝素蛋白纳米羟基磷灰石复合材料的生物安全性[J].中国组织工程研究,2016,20(38):5650-5656.FAN C Q,YE X J.Biological safety of silk fibroin/nanohydroxyapatite composites[J].Chinese Journal of Tissue Engineering Research,2016,20(38):5650-5656.
[52]刘浩,褚亚伟,丁涛,等.丝素蛋白/羟基磷灰石支架对成骨诱导脂肪干细胞活性及成骨性能的影响[J].中国组织工程研究,2015,19(45):7224-7229.LIU H,ZHU Y W,DING T,et al.Effect of silk fibroin/hydroxyapatite scaffold on the viability and osteogenic properties of adipose-derived stem cells under osteogenic induction[J].Chinese Journal of Tissue Engineering Research,2015,19(45):7224-7229.
[53]宁旭,庄勇,刘淼,等.丝素蛋白纳米羟基磷灰石复合材料制备及与脊柱的融合[J].中国组织工程研究,2017,21(2):221-226.NING X,ZHUANG Y,LIU M,et al.Preparation of silk fibroin/nano-hydroxypatite composite scaffold and its treatment outcomes in spinal fusion[J].Chinese Journal of Tissue Engineering Research,2017,21(2):221-226.
[54]MI R X,LIU Y X,CHEN X,et al.Structure and properties of various hybrids fabricated by silk nanofibrils and nanohydroxyapatite[J].Nanoscale,2016,8:20096-20102.
[55]李静静,朱海霖,雷彩虹,等.介孔生物玻璃/丝素蛋白复合多孔海绵的结构及止血性能研究[J].功能材料,2017,48(2):2097-2101.LI J J,ZHU H L,LEI C H,et al.Study on structure and hemostatic property of mesoporous bioglass/silk fibroin composite sponge[J].Journal of Functional Materials,2017,48(2):2097-2101.
[56]张静,肖长发,王健,等.丝素蛋白/聚丙烯腈共混膜的制备及性能研究[J].天津工业大学学报,2011,30(6):5-8.ZHANG J,XIAO C F,WANG J,et al.Study on preparation and properties of SF/PAN blend membranes[J].Journal of Tianjin Polytechnic University,2011,30(6):5-8.
[57]钱巧芬,张珊珊,侯静,等.乙二醇/丝素蛋白共混膜的研究[J].丝绸,2013,50(9):1-6.QIAN Q F,ZHANG S S,HOU J,et al.Study on ethylene glycol/silk fibroin blend membrane[J].Silk Monthly,2013,50(9):1-6.
[58]SUZUKI S,REBBACA A,TRAIAN V,et al.Treatment of silk fibroin with poly(ethylene glycol)for the enhancement of corneal epithelial cell growth[J].Journal of Functional Biomaterials,2015,6(2):345-366.
[59]王利君,熊杰,骆菁菁,等.聚乳酸-聚己内酯/丝素蛋白三元复合纳米纤维膜支架的结构与性能[J].纺织学报,2017,38(5):8-13.WANG L J,XIONG J,LUO J J,et al.Structure and properties of polylactic acid-polycaprolactone/silk fibroin composite nanofibrous scaffolds[J].Journal of Textile Research,2014,38(5):8-13.
[60]肖红伟,熊杰,李妮,等.聚乳酸-乙醇酸共聚物/丝素共混纳米纤维多孔膜的制备及性能[J].高分子材料科学与工程,2011,27(12):142-145.XIAO H W,XIONG J,LI N,et al.Preparation and properties of poly(lactide-co-glycolide)/silk blend nanofibrous porous membrane[J].Polymer Materials Science&Engineering,2011,27(12):142-145.
[61]PAN H,ZHANG Y P,HANG Y C,et al.Significantly reinforced composite fibers electrospun from silk finroin/carbon nanotube aqueous solutions[J].Biomacromolacules,2012,13:2859-2867.
[62]潘彩霞.丝素/碳纳米管及丝素/EDC复合膜的制备及性能研究[D].杭州:浙江大学,2016.PAN C X.Preparation and properties of SF/MWCNT and SF/EDC composites films[D].Hangzhou:Zhejiang University,2016.
[63]PAN H,ZHANG Y,HANG Y,et al.Significantly reinforced composite fibers electrospun from silk fibroin/carbon nanotube aqueous solutions[J].Biomacromolecules,2012,13(9):2859-2876.
[64]杨珊珊,沙金,赵海利,等.氧化石墨烯掺杂丝素蛋白复合材料的结晶和力学特性[J].高分子材料科学与工程,2016,32(1):46-51.YANG S S,SHA J,ZHAO H L,et al.Crystallinity and mechanical property of silk fibroin/graphene oxide composites[J].Polymer Materials Science&Engineering,2016,32(1):46-51.
[65]方洁羽,李延报,李怀栋.丝素-氧化石墨烯-甘油复合膜的制备及性能[J].功能高分子学报,2015,28(1):73-78.FANG J Y,LI Y B,LI H D.Preparation and properties of silk fibroin-graphene oxide-glycerin composite films[J].Journal of Functional Polymers,2015,28(1):73-78.
[66]ZHANG C,ZHANG Y P,LUO J,et al.Microstructural evolution of regenerated silk fibroin/graphene oxide hybrid fibers under tensile deformation[J].RSC Advances,2017,7(6):3108-3116.
[67]郭文君,吴建兵.丝素蛋白材料在药物控释中的应用[J].轻工科技,2017(5):23-24.GUO W J,WU J B.Application of silk fibroin material in controlled release of drugs[J].Light Industry Science and Technology,2017(5):23-24.
[68]YANG L,ZHENG Z Z,QIAN C,et al.Curcumin-functionalized silk biomaterials for anti-aging utility[J].Journal of Colloid and Interface Science,2017,496:66-77.
[69]张桂媛.丝素蛋白止血材料的制备及其性能研究[D].苏州:苏州大学,2015.ZHANG G Y.Preparation and properties of silk fibroin hemostatic material[D].Suzhou:Soochow University,2015.
[70]姚康德,尹玉姬.组织工程相关生物材料[M].北京:化学工业出版社,2003:100-120.YAO K D,YIN Y J.Biomaterials for tissue engineering[M].Beijing:Chemical Industry Press,2003:100-120.
[71]郭志豪,田林强,刘槃,等.丝素蛋白/介孔生物玻璃陶瓷复合材料修复颅骨缺损[J].中国组织工程研究,2017,21(2):192-196.GUO Z H,TIAN L Q,LIU P,et al.Repair of skull defects with silk fibroin/mesoporous glass ceramic composites[J].Chinese Journal of Tissue Engineering Research,2017,21(2):192-196.
[72]李明,莫茂松.基于石墨烯/丝素蛋白的骨组织工程三维多孔支架制备方法:CN201610097176.2[P].2016-06-15.LI M,MO M S.Preparation of three dimensional porous scaffold for bone tissue engineering based on graphene/fibroin:CN201610097176.2[P].2016-06-15.
[73]胡沐黎.丝素蛋白-磷酸钙类复合材料在椎体强化以及骨软骨修复中的应用研究[D].苏州:苏州大学,2016.HU M L.Research on the application of silk fibroin calcium phosphate composite in vertebral augmentation and osteochondral repair[D].Suzhou:Soochow University,2016.
[74]ZHANG Y Q.Natural silk fibroin as a support for enzymeimmobilization[J].Biotechnology Advances,1998,16(5):961-971.
[75]XU J,TANG T,ZHANG K,et al.Electroenzymatic catalyzed oxidation of bisphenol-a using HRP immobilized on magnetic silk fibroin nanoparticles[J].Process Biochemistry,2011,46(5):1160-1165.
[76]DELEZUK J,PAVINATTO A,MORAES M,et al.Silk fibroin organization induced by chitosan in layer-by-layer films:application as a matrix in a biosensor[J].Carbohydrate Polymers,2017,155:146-151.
[77]孙越宜,唐科梦,杨明英,等.丝素蛋白吸附金属离子的性能及其机理的研究[J].蚕桑通报,2016,47(2):26-31.SUN Y Y,TANG K M,YANG M Y,et al.Study of the adsorption and the mechanism of metal ions by fibroin[J].Bulletin of Sericulture,2016,47(2):26-31.
[78]李继丰,杨震,彭凤霞,等.羧基化棉织物的丝素蛋白接枝改性[J].印染,2015(7):1-6.LI J F,YANG Z,PENG F X,et al.Modification of carboxylated cotton fabric with silk fibroin[J].Shtexcloud,2015(7):1-6.
[79]李陈梅,熊佳庆,张德锁,等.丝素纳米银的制备及其对棉织物的改性实验研究[J].纺织导报,2014(1):102-105.LI C M,XIONG J Q,ZHANG D S,et al.Preparation of silk fibroin stabilized silver nanoparticles and their application for modification of cotton fabric[J].China Textile Leader,2014(1):102-105.
[80]赵林,林小贞,赖宏波,等.丝素蛋白/TiO2复合催化剂的制备及其可见光催化性能[J].分子催化,2014,28(3):275-281.ZHAO L,LIN X Z,LAI H B,et al.Preparation and visible light photocatalytic activity of silk fibroin/TiO2 nanocomposite[J].Journal of Molecular Catalysis,2014,28(3):275-281.
[81]MARELLI B,BRENCKLE M A,KAPLAN D L,et al.Silk fibroin as edible coating for perishable food preservation[J].Scientific Reports,2016,6:25263.
[82]屠洁,刘冠卉,燕薇.4种常用稳定剂和丝素蛋白对搅拌型酸奶黏度和保水性的影响[J].食品科学,2012,33(21):136-140.TU J,LIU G H,YAN W.Effects of four common stabilizers combined with silk fibroin on viscosity and water-holding capacity of stirred yogurt[J].Food Science,2012,33(21):136-140.
[83]赵应征,鲁翠涛,肖健,等.一种肌肤护理用丝素蛋白水凝胶及其制备方法:CN106420371A[P].2017-02-22.ZHAO Y Z,LU C T,XIAO J,et al.Preparation of skin care silk fibroin hydrogel:CN106420371A[P].2017-02-22.
[84]曹丽楠,徐保来.医用丝素蛋白皮肤再生膜的生物相容性评价[J].中国组织工程研究,2016,20(25):3653-3658.CAO L N,XU B L.Biocompatibility evaluation for skin reproducing membrane of medical fibroin[J].Chinese Journal of Tissue Engineering Research,2016,20(25):3653-3658.